The effects of nitrogen (N) fertiliser (0–200 kg/ha) on mass, botanical composition, and N concentration (%) in herbage were examined in nine 2- or 3-year rate × time of application experiments, 14 single-year annual rate of application experiments and 15 short-term spring rate of application experiments, at 27 sites in the Mt Lofty Ranges, South Australia, in 7 years between 1970 and 1979, inclusive. Effects on in vitro digestibility and concentrations of other nutrients in herbage were examined in selected experiments.Annual applications of 200 kg N/ha increased herbage mass by an average of 2.8 t/ha (57% increase), over the average yield of unfertilised pasture of 4.6 t/ha. Subterranean clover was eliminated from the sward with this rate of N application, although this may have been exacerbated by the experimental methods used. N fertiliser application increased herbage mass throughout the growing season, except in autumn 1972 when low rainfall restricted growth and about half of the experiments were not harvested. In 5 of the 126 individual harvests, herbage mass did not respond positively to N fertiliser applications, even though clover composition of herbage declined.A single application of 50 kg N/ha in autumn increased herbage mass, 6–8 weeks later, by an average 11�kg�DM/kg N, but this N effect only persisted to a subsequent harvest in about half of the experiments, with an average residual effect of 25%. Commonly, a response to N fertiliser in the first and/or second harvests was followed by a non-responsive period and then a depression in herbage mass, where no further N fertiliser was applied. With repeated N fertiliser applications, the average responses to 50� kg� N/ha were 11 kg DM/kg N in late winter and also in early spring, similar to the autumn response, and 18�kg�DM/kg N in late spring. In a later study, a single application of 50 kg N/ha in spring, for silage or hay conservation, increased herbage mass by an average of 1.3 t/ha in late spring while the average response to 100 kg N/ha was 2.0 t/ha. Clover composition declined but was rarely eliminated from the sward by these N rates when applied only in spring.From early winter to early spring, N concentration in herbage from unfertilised pasture ranged from 3 to 4% N and then progressively declined. Relationships between herbage N concentrations and increasing N rates were either linear or curvilinear in early and late winter, whereas in spring, many of these responses to N fertiliser were sigmoidal, with a decline in herbage N concentrations being observed at low N rates. Nitrogen fertiliser applied throughout the growing season had little effect on in vitro digestibility for a wide range of pasture compositions. However, in vitro digestibility of a pure grass pasture was increased early in the growing season by applications up to 50 kg N/ha, but was depressed by the same N rates applied in late spring. Consistently, an increase in N had the following effect on the concentration of other herbage nutrients: K�increased; Ca decreased becoming more pronounced as the growing season progressed; P decreased in late spring; and Cu fell in autumn. The content of these nutrients in harvested herbage usually increased with increasing N rate, particularly when associated with large herbage mass responses to N fertiliser. The K : (Ca + Mg) ratio in herbage, a criterion for grass tetany, increased detrimentally with increasing N rate. Strategies are proposed for using N fertiliser on rain-fed pasture in the Mt Lofty Ranges.
Interploid Hybridization of Cucumis melo and C. metuliferus