scholarly journals Comparative Assessment of Trace Elements in the Blood of Gastric Cancer Patients and Healthy Subjects

2020 ◽  
Vol 11 (3) ◽  
pp. 10824-10843
Keyword(s):  
Gastric Cancer ◽  
Trace Elements ◽  
Healthy Subjects ◽  
Absorption Spectrometry ◽  
Correlation Study ◽  
Acid Mixture ◽  
Flame Atomic Absorption ◽  
Healthy Donors ◽  
Trace Elemental ◽  
Gastric Cancer Patients

Gastric cancer (GC) remains one of the most common malignancies globally, particularly in developing countries. Environmental contaminants such as trace elements are known to involve in a plethora of diseases, including cancer. The present study was carried out to find out the imbalances in trace elemental levels (Cd, Cr, Cu, Fe, Mn, Pb, and Zn) in the blood of GC patients in comparison with and healthy donors. The blood samples were digested in a nitric acid-perchloric acid mixture. The elemental levels were quantified by flame atomic absorption spectrometry. Significantly lower concentrations of zinc, iron, lead, cadmium, and chromium were found in the blood of GC patients than the controls. A correlation study revealed diverse relationships among the elements in the blood of cancerous patients and controls. Considerable variations in the elemental concentrations were observed with the gender, food habits, habitat, and smoking habits of the donors. Elemental contents revealed significant differences with the stages (I, II, III & IV) and types (adenocarcinoma & gastrointestinal stromal tumor) of GC patients. Multivariate analysis exhibited significantly divergent apportionment of the patients' elemental levels and healthy subjects, which may be linked with the altered metabolism of the elements in GC patients.

Selected Trace Elements and Heavy Metals in the Serum of Postoperative Gastric Cancer Patients and Their Relationship to CEA

2021 ◽  
Vol 53 (03) ◽  
pp. 220-226
Author(s):  
Abbas Mohsin Abbas ◽  
Keyword(s):  
Gastric Cancer ◽  
Positive Association ◽  
Absorption Spectrometry ◽  
Serum Levels ◽  
The Body ◽  
Metal Exposure ◽  
Flame Atomic Absorption ◽  
Digestion Technique ◽  
Metal Levels ◽  
Gastric Cancer Patients

Introduction: Gastric cancer (GC) is the second and fourth most prevalent cancer in men and women, respectively, and is one of the leading causes of cancer death worldwide. Many studies have shown that heavy metal exposure and trace element levels in the body are the most critical etiologies for cancer development. As a result, the goal of our research was to assess the imbalances in the serum concentrations of selected elements (Cu, Co, Se, Ni, Cd, and Pb) in post-operative GC patients against healthy participants/ controls. Methods: The metal levels were determined using a nitric acid/perchloric acid-based wet digestion technique and flame atomic absorption spectrometry, Serum levels of CEA were measured using a two-site immunoenzymometric assay, which is performed entirely in the AIA-PACK SLa test cups. Results: Pb, Cd, and Ni concentrations were found to be significantly higher in the blood of GC patients than in the blood of controls, but Cu and Co levels were significantly lower in the blood of GC patients than in the blood of controls. In the blood of post-operative GC patients, correlation analysis revealed a positive association between CEA-Cd, CEA-Pb, and CEA-Ni, while the correlation was negative for CEA-Cu. These findings were statistically significant (p < 0.05). Conclusion: According to our findings, low Co and Se levels, as well as high Pb and Cd levels, may have a role in the development of stomach cancer.

Online Preconcentration System Using 6-(2-Thienyl)-2-Pyridine Carboxaldehyde Functionalized Amberlite XAD-4 Resin for the Determination of Trace Elements in Waters by Flame Atomic Absorption Spectrometry

2013 ◽  
Vol 96 (3) ◽  
pp. 642-649 ◽  
Author(s):  
Cennet Karadaş ◽  
Derya Kara
Keyword(s):  
Trace Elements ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Flame Atomic Absorption Spectrometry ◽  
Water Samples ◽  
Certified Reference Materials ◽  
Absorption Spectrometry ◽  
Rice Flour ◽  
Flame Atomic Absorption

Abstract An online flow injection method was developed for the determination of trace elements in waters using 6-(2-thienyl)-2-pyridinecarboxaldehyde functionalized Amberlite XAD-4 resin. The metals were eluted with 1.0 M HNO3 directly to a flame atomic absorption spectrometry determination system. The sorption capacities of the resin for metal ions were 74.6, 113.7, 48.3, 131.6, and 88.6 μmol/g for Cd(II), Ni(II), Pb(II), Co(II), and Cu(II), respectively. The resin was found to be very stable and capable of being used for more than 250 retention and elution cycles without major loss of performance. Detection limits (3SD) were determined to be 0.14 μg/L for Cd, 0.39 μg/L for Cu, 0.82 μg/L for Ni, 0.88 μg/L for Co, and 2.54 μg/L for Pb using 10 mL sample loading. The accuracy of the proposed method was checked using different certified reference materials [estuarine water (SLEW-3), riverine water (SRLS-5), 19-element aqueous standard (QCS-19), wheat flour (NBS 1567 a), and rice flour unpolished high level Cd (NIES 10c)]. Results were in agreement with certified values. The proposed method was applied to the determination of Cu, Ni, Co, Cd, and Pb in natural water samples. The recovery values for spiked water samples were between 91.9 and 107.4%.

scholarly journals The concentrations of Fe, Cu and Zn in selected wines from South-East Serbia

2010 ◽  
Vol 75 (12) ◽  
pp. 1701-1709 ◽  
Author(s):  
Danijela Kostic ◽  
Snezana Mitic ◽  
Gordana Miletic ◽  
Sasa Despotovic ◽  
Aleksandra Zarubica
Keyword(s):  
Developing Countries ◽  
Trace Elements ◽  
Fruits And Vegetables ◽  
Absorption Spectrometry ◽  
Human Consumption ◽  
Flame Atomic Absorption ◽  
East Region ◽  
Essential Trace Elements ◽  
The World ◽  
Cu And Zn

Fruits and vegetables constitute the cheapest source of essential trace elements for the majority of people living in developing countries. The Cu, Fe and Zn contents in twenty selected wine samples produced in the South-East region of Serbia were determined by flame atomic absorption spectrometry. The Cu concentrations varied from 0.07 to 0.57 ppm in wines, and the Fe concentrations fluctuated from 2.93 to 36.2 ppm, while the Zn levels were in the range from 0.21 to 0.67 ppm. The established contents of Cu and Zn showed that wines from this part of the world could serve as good dietary sources of the essential trace metals, and the determined values were within the allowed metals levels in wines for human consumption.

scholarly journals Multi-Element Determination of Trace Elements in B7-diesel Oil by High-Resolution Continuum Source Flame Atomic Absorption Spectrometry

2021 ◽  
Vol 8 ◽  
Author(s):  
Heldiane S. dos Santos ◽  
Alexandre de Jesus ◽  
Diane O. Laroque ◽  
Clarisse M. S. Piatnicki ◽  
Márcia M. da Silva
Keyword(s):  
Trace Elements ◽  
High Resolution ◽  
Atomic Absorption Spectrometry ◽  
Flame Atomic Absorption Spectrometry ◽  
Absorption Spectrometry ◽  
Diesel Oil ◽  
Element Determination ◽  
Continuum Source ◽  
Flame Atomic Absorption

In this work, a method for sequential multi-element determination of Cu, Fe, Ni, Pb, Zn, Al, Cr and Sn in B7-diesel oil samples by high-resolution continuum source flame atomic absorption spectrometry (HR-CS F AAS) was proposed. The sample preparation was based on formation of microemulsion (ME), which was investigated through a three-phase diagram. The ME composition adopted was B7-diesel oil (3.3 g), aqueous phase containing nitric acid (1.4 mol L-1, 300 μL) and n-propanol (up to 10 mL). The use of surfactant Triton X-100 in the formation of ME was also evaluated. Limits of detection in the range of 0.01 – 0.4 mg kg-1 were obtained. Spike-recovery tests were accomplished and the results varied between 93 and 124%. The accuracy of the proposed method was confirmed by the analysis of certified reference material (CRM) NIST SRM 1084a (Wear-Metals in Lubricating Oil); there was no statistical difference between the obtained results and the certified values (at 95% confidence level). Twelve B7-diesel oil samples were analyzed and Cu, Fe and Cr were found in three samples. The proposed method was simple, fast and accurate. The sequential multi-element determination of trace elements presented advantages as low costs and reduction in analysis time, being appropriate for routine analysis.

SOLUBLE FORMS OF PD-1 AND PD-L1 IN BLOOD PLASMA OF GASTRIC CANCER PATIENTS AND THEIR ASSOCIATIONS WITH CLINICAL AND MORPHOLOGICAL CHARACTERISTICS OF THE DISEASE

2020 ◽  
Vol 65 (6) ◽  
pp. 347-352 ◽  
Author(s):  
E. S. Gershtein ◽  
N. A. Ognerubov ◽  
V. L. Chang ◽  
V. V. Delektorskaya ◽  
E. A. Korotkova ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Blood Plasma ◽  
Cancer Patients ◽  
Cell Cancer ◽  
Morphological Characteristics ◽  
Control Group ◽  
Tnm System ◽  
Healthy Donors ◽  
Gastric Cancer Patients ◽  
In Blood Plasma

Results of comparative ELISA investigation of pretreatment sPD-1 and sPD-L1 content in blood plasma of 100 gastric cancer patients at various disease stages aged 25 to 81 years are presented. Control group included 60 practically healthy donors aged 18 - 68 years. Plasma sPD-L1 concentrations did not differ between gastric cancer patients and control group, and sPD-1 levels were statistically significantly lower in patients than in healthy donors (p<0.0001). Positive correlation (R=0.38; p=0.003) was revealed between plasma sPD-1 and sPD-L1 levels in control group and negative (R= -0.26; p=0,009) - in gastric cancer patients. ROC curve revealed the best sPD-1 cut-off level (< 21 pg/ml) with 77% sensitivity and 63.3% specificity, which is not sufficient for its application as diagnostic marker. Statistically significant increase of plasma sPD-L1 from stage I to stage IIIC (R=0.50; p=0.000011) was found. Analysis of associations between the evaluated markers’ levels and indices of gastric cancer expansion according to TNM system revealed statistically significant positive associations of plasma sPD-L1 levels with T (tumor invasion depth) and N (number of affected lymph nodes) indices: R=0.33; p=0.00093, and R=0.27; p=0.0099 respectively. sPD-L1 level was significantly increased in patients with low differentiated adenocarcinoma and cricoid-cell cancer as compared to highly differentiated adenocarcinoma (p=0.02 and p=0.004 respectively); in patients with cricoid-cell cancer it was also higher than in those with moderately differentiated adenocarcinoma (p=0.043) and undifferentiated cancer (p=0.049). Plasma sPD-1 level did not depend on disease stage, TNM system indices and tumor histological structure. Thus, soluble ligand sPD-L1, but not its receptor sPD-1, plasma level is increased in patients with unfavorable clinical and morphological characteristics, may be regarded as potentially valuable prognostic factor for gastric cancer patients’ survival, and probably as a predictor of anti - PD-1/PD-L1 treatment efficiency.

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