scholarly journals Impacts of Mycoplasma gallisepticum Vaccine on Newcastle Disease Vaccination and Protection in Parent Stock Flocks

1970 ◽  
Vol 24 (1) ◽  
pp. 62-64
Author(s):  
M Rayhan Faruque ◽  
Jens P Christensen

The present study reports on the impact of Mycoplasma gallisepticum (MG) vaccination on vaccine respond and subsequent protection against Newcastle disease (ND) in parent stock flocks of Department of Livestock Services (DLS) using MG killed vaccine and conventional ND vaccines. Birds were grouped into four groups, each consisted of 50 birds from the same flock. Group A birds were vaccinated with ND, group B with ND and MG, group C with MG, and group D birds were kept as unvaccinated control. The parameters studied included detection of ND antibody, MG seroprevalance, mortality (%), and cause of death. The sera of groups B and C were sero-positive after administration of MG vaccine. The haemagglutination-inhibition (HI) titres of group A were higher than group B from week 4 after administration of MG vaccine to the birds of group B. The mortality was very low; one bird of group C died at week 5 due to traumatic injury and another bird of group D died at week 2 due to infectious bronchitis virus (IBV). After challenge, birds of groups A and B showed no clinical signs and normal post mortem findings were found. Birds of groups C and D showed clinical signs from day 3 and different pathological lesions were found in post mortem. The MG vaccination did not improve other parameters. Therefore, inoculation of MG INAC vaccine is not justified and is too expensive at farm levels.Keywords: Mycoplasma gallisepticum (MG) vaccine, Newcastle disease (ND) vaccination, Protection, MortalityDOI: http://dx.doi.org/10.3329/bjm.v24i1.1240   Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 62-64 

2019 ◽  
Vol 10 (3) ◽  
pp. 536-551
Author(s):  
Heidi Amezcua Hempel ◽  
María Salud Rubio Lozano ◽  
Eliseo Manuel Hernández Baumgarten ◽  
Pablo Correa Girón † ◽  
Oscar Torres Ángeles ◽  
...  

The study was to determine the presence of Classical Swine Fever virus (CSFv), in the meat of vaccinated pigs with the PAV-250 strain and then challenged using the same strain. Five treatment groups were established (each with four pigs). Group A: Pigs thatwere fed with processed hams from negative animals; Group B: Pigs that were fed with processed hams from commercial pigs inoculated with the ALD (reference strain) (titre of 104.0/ml); Group C: Pigs fed with processed hams from pigs infected with the virulent ALD strain (titre of 102.5/ml); Group D: Pigs fed with processed hams from pigs vaccinated with the PAV-250 strain and challenged with the ALD strain (titre of 101.1/ml); and Group E: Pigs fed with processed hams from pigs vaccinated with two doses of the PAV-250 strain and challenged with the ALD strain (negative). Blood samples were taken at d 1, 5, 10, 15 and 20 for biometric analysis. Groups B, C and D manifested clinical signs of CSFv: 40 °C temperature, anorexia, paralysis, vomiting, diarrhea, tremor, hirsute hair and cyanosis. Pigs were slaughtered and necropsies performed to identify lesions in tissues. Results of direct immunofluorescence testing of tissues were positive and the virus was recovered. Under these study conditions, it was found that CSFv resisted the cooking method at 68 °C for 40 min in hams from unvaccinated pigs, and that the virus was able to transmit the disease to healthy unvaccinated pigs, whereas the hams from the vaccinated animals did not transmit the virus.


2019 ◽  
Vol 61 (1) ◽  
Author(s):  
Christin Röttiger ◽  
Maren Hellige ◽  
Bernhard Ohnesorge ◽  
Astrid Bienert-Zeit

Abstract Background The use of cadavers for radiology research methodologies involving subjective image quality evaluation of anatomical criteria is well-documented. The purpose of this method comparison study was to evaluate the image quality of dental and adjacent structures in computed tomography (CT) and high-field (3 T) magnetic resonance (MR) images in cadaveric heads, based on an objective four-point rating scale. Whilst CT is a well-established technique, MR imaging (MRI) is rarely used for equine dental diagnostics. The use of a grading system in this study allowed an objective assessment of CT and MRI advantages in portraying equine cheek teeth. As imaging is commonly performed with cadaveric or frozen and thawed heads for dental research investigations, the second objective was to quantify the impact of the specimens’ conditions (in vivo, post-mortem, frozen-thawed) on the image quality in CT and MRI. Results The CT and MR images of nine horses, focused on the maxillary premolar 08s and molar 09s, were acquired post-mortem (Group A). Three observers scored the dental and adjacent tissues. Results showed that MR sequences gave an excellent depiction of endo- and periodontal structures, whereas CT produced high-quality images of the hard tooth and bony tissues. Additional CT and MRI was performed in vivo (Group B) and frozen-thawed (Group C) in three of these nine horses to specify the condition of the best specimens for further research. Assessing the impact of the specimens’ conditions on image quality, specific soft tissues of the maxillary 08s and 09s including adjacent structures (pulps, mucosa of the maxillary sinuses, periodontal ligament, soft tissue inside the infraorbital canal) were graded in group B and C and analysed for significant differences within CT and MR modalities in comparison to group A. Results showed that MRI scores in vivo were superior to the post-mortem and frozen-thawed condition. Conclusions On comparing the imaging performance of CT and MRI, both techniques show a huge potential for application in equine dentistry. Further studies are needed to assess the clinical suitability of MRI. For further research investigations it must be considered, that the best MR image quality is provided in live horses.


2019 ◽  
Vol 39 (11) ◽  
pp. 863-869
Author(s):  
Thaiza Savaris ◽  
Claudia P. Biffi ◽  
Daiane Ogliari ◽  
Nathalia Wicpolt ◽  
Franciéli Adriane Molossi ◽  
...  

ABSTRACT: Crotalaria lanceolata E. Mey. and Crotalaria pallida Aiton. are leguminous plants of family Fabaceae found in most of the Brazilian territory. They were initially used as green manure and due their easy spread they are currently considered weeds in crops. Soybean and corn contamination can occur through the mechanical harvesting of these grains along with seeds of the Crotalaria species, which end up in the formulation of feed for production animals. Crotalaria spp. genus has toxic pyrrolizidine alkaloids (PA). Most plant species belonging to this genus can cause acute or chronic liver injury. In a first stage, one-day old broilers were divided into three groups: Group A (C. pallida seeds), Group B (C. lanceolata seeds), and Group C (Control). Groups A and B were divided into five subgroups, each with eight broilers, which received the following doses of the respective seeds in feed as of the 7th day of age: daily doses of 0.4%, 0.8% and 2.5%, and single doses of 15% and 25%. Four broilers in each study group were euthanized at 28 days of age - completing 21 days of seed consumption, and the four remaining broilers were euthanized at 42 days of age - completing 35 days of seed consumption. In a second stage, experiments were conducted using seeds of both the aforementioned plants with 28-day old broilers. These were divided into three groups of four animals each: Group D (C. pallida seeds) and Group E (C. lanceolata seeds), which received the respective seeds at daily doses of 1% and 2% in feed for 20 days, and Group F (Control). These broilers were euthanized when they were 80 days old. C. lanceolata seeds showed higher toxicity to broilers than C. pallida seeds, both supplied as of the 7th day of life. Clinical signs included inappetence, ruffled feathers, and brown diarrhea. The following gross lesions were observed: subcutaneous edema, ascites, hydropericardium, yellowish liver with hypertrophy or atrophy and enhanced lobular pattern, and distended gallbladder. Histologic lesions present in all birds in varying degrees were characterized by tumefaction and vacuolar degeneration of hepatocytes. The following clinical conditions and gross lesions were observed in the broilers: hepatocyte megalocytosis and karyomegaly, slight biliary epithelial hyperplasia, eosinophilic spheroids, and nuclear invagination with loss of hepatocyte cord architecture.


Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 5827-5827
Author(s):  
Jiayi WANG ◽  
Yingyi He ◽  
Zhimin Liang ◽  
Tiezhen Ye ◽  
Hui Zhang

Abstract Background: Palliative care is becoming more and more important for systemic cancer treatment in developed countries, while this remains infamous topic in developing countries, such as in China. Contemporary treatment strategies have greatly contributed to the improved outcome in childhood cancer patients, survivorship comes at the cost of developing some treatment-related health condition, such as pain-related depression, chronic pain etc. Thus, developing a well-tolerated pain control methods is of great importance within the cancer treatment. Objective: To evaluate the impact and outcome of different pain control applications on invasive procedure in children with leukemia, and record the adverse reactions. Methods: The enrollment of childhood leukemia patients in our hospital from November 2011 to November 2016 were divided into four groups, that is successively midazolam + local anesthesia (group A), midazolam + ketamine +local anesthesia (group B), midazolam + fentanyl + local anesthesia (group C), and fentanyl + propofol + local anesthesia (group D). The efficacy and adverse reactions were systemically recorded. The inter-group diffferences were calculated using x2 test. Results: No significancewas observed in age, gender, and disease distribution in these four groups by ANOVA ONEWAY analysis. The sedation outcome is more pronunced in group D than others. Also, the quality of procedural pain control in group D was the best (P<0.01). In terms of the analgesic effect, group B and D were better than that group A and C. There was significant difference in Hallucination was more easily detected in group B and C. Systemic recovery was delayed in group B other than group A, C, and D. Basing on the survey, we did found that the family members were more willing to accept pain control treatment for their sick kids under the safety assurance. The compliance was significantly improved in group D. Conclusion: Upon adequate auxiliary breathing preparation and rigorous monitor, propofol combined with low-dose fentanyl was the best sedative/analgesic option for pain control within leukemia patients receiving invasive procedure.The outcome of propofol combined with low-dose fentanyl wasvery safe, satisfactory and compliable. Up to now, this study is the first pain control study for invasive procedure in China mainland, it deserves being paid attention. Disclosures No relevant conflicts of interest to declare.


2020 ◽  
Vol 3 ◽  
pp. 17-23
Author(s):  
Rabia Sabir

The goal of current research was production of bivalent adjuvant based inactivated I-2 strain of Newcastle Disease (ND) and Avian Influenza (AI) vaccine and to compare efficacy with commercially available bivalent inactivated vaccine. Experiment was carried out on one-day-old broiler chicks by dividing them into 3 groups A, B & C. Vaccine was injected on 7, 14, 21 and 28 day. Blood samples were collected on 7, 14, 21, 28 and 35 day. Results of Hemagglutination Inhibition (HI) test presented the geometric mean titers of group A and B were <1:4, 1:18.8, 1:115.2, 1:358.4, 1:128 & <1:4, 1:8.4, 1:36.8, 1:56, 1:22.4 respectively for AI. Similarly the geometric mean titers of group A and B were <1:4, 1:19.6, 1:89.6, 1:320, 1:115.2 & <1:4, 1:9.2, 1:25.6, 1:36.8, 1:22.4, respectively for ND. However, control group showed <1:4 geometric mean titer. The challenge was given on 28th day and birds were monitored for next 7 days for clinical signs. The chicks of group A showed no clinical signs of diseases (AI, ND). However, in group B 60% morbidity and 90% mortality was recorded on 4th and 6th day of post challenge, respectively. While 100% mortality was recorded in control group.


2018 ◽  
Vol 90 (1) ◽  
pp. 44 ◽  
Author(s):  
Rossella Mazzilli ◽  
Gloria Angeletti ◽  
Soraya Olana ◽  
Michele Delfino ◽  
Virginia Zamponi ◽  
...  

Objectives: The aim of this study was to assess the prevalence of patients with Erectile Dysfunction (ED) receiving psychotropic drugs, the impact of these drugs on hormonal profile, and the efficacy of PDE5-i in these patients. Materials and methods: We recruited 1872 patients referring for ED to our Andrology Unit. Assessment included serum testosterone, gonadotropins, TSH, prolactin, and PSA, and the IIEF-5 questionnaire for ED diagnosis. Inclusion criteria were age 21-75 years and IIEF-5 total score ≤ 21; exclusion criteria included hypogonadism, diabetes mellitus, previous prostatectomy, other medication intake, and ED diagnosis prior to psychotropic drug treatment. Efficacy was rated with the IIEF-5 (remission: total score ≥ 22). Results: The prevalence of ED patients treated with psychotropic drugs since ≥ 3 months was 9.5% (178/1872), subdivided according to the drugs used into: Group A, 16 patients treated with atypical antipsychotics (9.0%); Group B, 55 patients with benzodiazepines (30.9%); Group C, 33 patients with antidepressant drugs (18.5%); and Group D, 74 patients with multiple psychotropic drugs (41.6%). Patients in Group A were significantly younger than other groups (p < 0.05). The hormonal profile presented only higher prolactin level in patients treated with antipsychotics, alone or in combination (p < 0.05). Overall, 146 patients received PDE5-i. Remission rate, after three months of treatment, was significantly higher in Group B compared to C and D groups (p < 0.05). Conclusions: A substantial portion of patients receiving psychotropic drugs show ED. Sexual performance in these patients benefits from PDE5-i. Age, effects of psychiatric disorders, psychotropic drugs, and PDE5-i treatment modality accounted for variability of response in this sample.


2014 ◽  
Vol 24 (1-2) ◽  
pp. 79-84
Author(s):  
MS Islam ◽  
MS Parvin ◽  
J Akhter ◽  
MT Islam ◽  
MP Siddique ◽  
...  

The efficacy of hyperimmune serum for the treatment of Newcastle disease (ND) was evaluated in indigenous birds. A total of 20 indigenous birds (3 to 4 months old) were divided into three groups, namely group A (n = 9), B (n = 8) and C (n = 3). Birds of all the groups were infected orally with 0.2 mL (106.5EID50/0.1 mL) of virulent Newcastle disease virus (NDV). Birds of group A were treated with hyperimmune serum (raised in chickens against NDV) @ 2 mL/bird IM after 6 hours of infection before commencement of clinical signs. Birds of group B received two doses of hyperimmune serum, first @ 2 mL/bird IV after commencement of clinical signs and second @ 3 mL/bird IM 5 hours after first dosing. Birds of group C served as infected control. Birds of group A did not show any clinical signs of ND except one (11%). However, the only sick bird recovered after one repeat dosing of 1 mL hyperimmune serum. In group B, 87.5% birds survived with two birds having nervous signs. Therefore, it may be concluded that hyperimmune sera was effective in preventing morbidity and mortality due to ND in birds when administered before or after commencement of the clinical signs. However, it is needed to conduct a field trial to explore the efficacy of hyperimmune sera raised both in chickens and rabbits.DOI: http://dx.doi.org/10.3329/pa.v24i1-2.19103 Progress. Agric. 24(1&2): 79 - 84, 2013


2012 ◽  
Vol 29 (1) ◽  
pp. 52-57 ◽  
Author(s):  
J C Papakostas ◽  
E Douitsis ◽  
I Sarmas ◽  
S Avgos ◽  
A Kyritsis ◽  
...  

Objectives To examine and compare the effects of downwards versus upwards total stripping of great saphenous vein (GSV) on saphenous nerve (SN) injury using clinical and electrophysiological studies. Methods Fifty patients with varicosities were equally and randomly assigned to undergo total, upwards stripping (group A) or downwards stripping (group B) of GSV during saphenectomy. SN function was measured with electroneurogram (ENG) before operation, two weeks and 12 weeks after, in order to record the incidence and type of SN injury. Clinical signs of SN injury were also recorded at the same time points. The results were statistically analysed. Results There were no statistical significant differences on the occurrence of SN injury between groups A and B at two and 12 weeks, respectively, as confirmed with ENG studies and clinical evaluation. There were no differences between the two techniques with regard to the type of SN injury. SN injury was significantly ameliorated from 34% to 6% during the first three months. Conclusion SN injury was equally observed after downwards or upwards total stripping of the GSV, as confirmed by ENG and clinical evaluation, with no differences in injury type. SN injury tends to be relieved through time in most patients.


2011 ◽  
Vol 2 (1) ◽  
pp. 39-43
Author(s):  
Shobha Deshpande ◽  
Santosh M Sholapurmath ◽  
Shigli L Anand

ABSTRACT Background and objective The effect of teething on infants health has been debated for at least 5000 years and traditional beliefs on issue have still not been entirely supplemented by scientific finding. This study has tried to gather scientific information of systemic/clinical signs and symptoms in scientific manner. The objectives of this study were: (1) To describe the most frequent objective clinical manifestation during the eruption of primary teeth, e.g. fever, diarrhea, drooling, fever-drooling, fever-diarrhea, drooling-diarrhea. (2) To assess the correlation between systemic symptoms and eruption of various group of teeth, e.g. incisor, canine, molar. Methods The survey was conducted in 500 children between 5 and 30 months and registered at child development center, KLES's Hospital and Medical Research Center, Nehru Nagar, Belgaum, Karnataka. A specially structured questionnaire was applied with face-to-face interview. Information was relayed in yes or no manner of objective manifestation during eruption of primary incisor, canine and molar which included drooling, diarrhea, fever and combination of these symptoms. Data were analyzed by descriptive statistic and Chi-square analyses. Results The study showed more number of children in group A (less than 12 months) manifested drooling symptoms. As the ages advances with group B (between 12 and 18 months), group C (between 18 and 24 months) and group D (above 24 months), clinical manifestation of saliva found to be reduced. The results of study showed that 87.78%; 57.8%; 57.55%; 25.7%; of children with erupting teeth demonstrated general clinical symptoms like drooling, diarrhea and fever. The presence of fever-diarrhea in this study within group A, group B, group C and group D showed highest number of children by the manifestation than any other codes evaluated in the study. Interpretation and conclusion Present data demonstrate association between fever-diarrhea observed in all group consistently when marked numerically. As the age advances the fever-diarrhea from group C (between 18 and 24 months) and group D (above 24 months) rank second and third. This ranking in group A (less than 12 months) and group B (between 12 and 18 months) at its highest position and attributable to lower immunity of infants. Further, virology study should be conducted on subjects to demonstrate any possible occurrence of systemic disturbances.


1970 ◽  
Vol 27 (1) ◽  
pp. 1-5
Author(s):  
S Kamrunnahar ◽  
MM Alim ◽  
AHM Taslima ◽  
MA Islam ◽  
NC Paul

A comparative efficacy of four lyophilized Newcastle disease vaccine, namely, Medivac ND-LaSota®, BCRDV®, Izovac B1 Hitchner® and Cevac Vitapest-L® in regard to the production of Himagglutination Inhibition (HI)- antibody was accomplished. For this, a total of 75 chicks were equally divided into five experimental groups such as, A, B, C, D and E. The HI antibody titers of group A vaccinated on 5 and 21 days of age with Medivac NDLaSota ® fluctuated among the Mean ± SD of 89.60 ± 33.05, 102.40 ± 33.05 and 192 ± 67.46 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. As regards, group B administered with BCRDV® on the same days, the HI titers varied with the Mean ± SD of 83.20 ± 30.91, 102.40 ± 33.04 and 204.80 ± 66.09 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. When considered the HI antibody titers of group C inoculated with Izovac B1 Hitchner® on days 5 and 21 of age, HI titer exhibited the Mean ± SD of 80.00 ± 43.33, 96.00 ± 33.73 and 192.00 ± 67.46 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. Elucidation of HI antibody titers of group D birds receiving Cevac Vitapest-L® on same days were recorded to be of Mean ± SD of 96.00 ± 33.73, 115.20 ± 26.98 and 320.00 ± 173.31 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. In birds of unvaccinated control group E, HI titers were found to be ranged from 32- 64, 16-32, 8-16, 4-8 and 2-4 with Mean ± SD of 48.00 ± 16.87, 24.00 ± 8.43, 11.20 ± 4.13, 5.20 ± 1.93 and 3.40 ± 0.97 on 3, 15, 17, 19 and 31 days of age respectively. Thus, it was found that birds of group A, B, C and D vaccinated with Medivac ND-LaSota®, BCRDV®, Izovac B1 Hitchner® and Cevac Vitapest-L® induced slightly higher level of HI antibody titers than that of BCRDV. Maternally derived antibody (MDA) persisted to a minimal level until the age of chicks of day 17 and later on MDA declined. Keywords: Vaccine; Antibody Titer; Newcastle Disease VirusDOI: http://dx.doi.org/10.3329/bjm.v27i1.9159 BJM 2010; 27(1): 1-5


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