scholarly journals Comparative study of rapid urease test and histopathological examination for detection of H. pylori infection

2017 ◽  
Vol 4 (12) ◽  
pp. 4071
Author(s):  
Virendra S. Athavale ◽  
Vinit Singh ◽  
Shivmurti N. Khandalkar ◽  
Dakshayani S. Nirhale ◽  
Aditya Lad ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Good Alternative ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Study Group ◽  
Medical College ◽  
Urease Test ◽  
Resource Poor ◽  
H Pylori

Background: Aims and objectives of the study was to study the incidence of H. pylori infection in our Hospital. To find out the sensitivity and specificity of rapid urease test (RUT) and histopathological examination for the detection of H. pylori on gastric biopsy. To study the effect of anti H. pylori drugs by performing follow-up endoscopy in terms of positive or negative Rapid Urease Test and Histopathological Examination report.Methods: The study was conducted at Dr. D. Y. Patil Medical College and Hospital, DPU University, for a period of 2 years (from July 2012-September 2014) and is a prospective and comparative randomized type of study using 100 patients. The study was approved by the Institute’s Ethics Committee.Results: Data analysis showed that: In our study 84 patients (84%) were detected positive by RUT method. In our study 83 patients (83 %) were detected positive by HPE method. There was an association between RUT and HPE finding in study group with the sensitivity being 96.38 % and specificity being 74.47 %, PPV was 95.24% and NPV was 81.25%. At the time of follow up after taking anti H pylori treatment, 79 patients were RUT negative and 8 were RUT positive who were defaulters (not taken complete dose) and again started on treatment with the accuracy of the drug being 98.85 %. At the time of follow up after taking anti H pylori treatment 78 patients were HPE negative and 9 were HPE positive who were defaulters (not taken complete dose) and again started on treatment. There was an association between RUT and HPE finding at follow up with the sensitivity being 88.89 % and specificity being 100%, PPV was 100% and NPV was 98.73%.Conclusions: Our study reveals that RUT is accurate for the diagnosis of H. pylori infection and its use will serve as a good alternative to histology in management of patients with dyspepsia in resource poor environments, except in patients who need histology for reasons other than H. pylori diagnosis.

scholarly journals Urine-Based ELISA for the Detection of Helicobacter pylori IgG Antibody and Comparison with Other Invasive Methods.

1970 ◽  
Vol 4 (1) ◽  
pp. 14-17 ◽  
Author(s):  
Md Din-ul Islam ◽  
Sufi HZ Rahman ◽  
SM Shamsuzzaman ◽  
Naima Muazzaman ◽  
Nasim Ahmed ◽  
...  
Keyword(s):  
Endoscopic Biopsy ◽  
Rapid Urease Test ◽  
Igg Antibody ◽  
Medical College ◽  
Urease Test ◽  
Study Population ◽  
H Pylori ◽  
Sensitivity Specificity ◽  
Culture Positive ◽  
Upper Gastrointestinal

The present study was conducted in the department of Microbiology, Dhaka Medical College, Dhaka during the period of January, 2007 to December, 2007. Urine samples were collected from 86 dyspeptic patients undergoing upper Gastrointestinal Tract (GIT) endoscopy to determine anti-H. pylori IgG antibody by an ELISA method. Gastric biopsy tissues were tested for culture, rapid urease test and H&E/Giemsa stain. Out of 86 endoscopic biopsy specimens, 45 (52.33%) were culture positive, 63 (73.26%) were rapid urease test positive and 64 (74.42%) were H&E/Giemsa stained positive for H. pylori. According to operational standard definition, among the 86 study population, 66 (76.74%) were H. pylori infected, 16 (18.60%) were uninfected and 4 (4.65%) were indeterminate. Among 66 H. pylori infected cases, 63 (95.45%) were urine ELISA positive and among 16 uninfected cases 3 (18.75%) were urine ELISA positive. Out of 86 study population, 66 (76.74%) were urine ELISA positive. The sensitivity, specificity, PPV, NPP and accuracy of urine ELISA were 95.45%, 81.25%, 95.45%, 81.25% and 92.68% respectively. The result of the study shows that H. pylori infection can be rapidly and reliably diagnosed by detecting anti-H. pylori IgG from urine. Key words: H. pylori; ELISA; GIT; IgG. DOI: http://dx.doi.org/10.3329/bjmm.v4i1.8463 BJMM 2011; 4(1): 14-17

scholarly journals Prevalence of Helicobacter pylori in patients with peptic ulcer disease

2018 ◽  
Vol 5 (4) ◽  
pp. 1315
Author(s):  
Mrutyunjay I. Uppin ◽  
Kapildev K. Hannurkar
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Peptic Ulcer Disease ◽  
Histopathological Examination ◽  
Rapid Urease Test ◽  
Duodenal Ulcers ◽  
Ulcer Disease ◽  
Urease Test ◽  
H Pylori ◽  
Upper Gastrointestinal

Background: Prevalence of Helicobacter pylori (H. pylori) emerges throughout the world and instigates peptic ulcer disease (PUD). The study was conducted with the aim to determine the prevalence of H. pylori in patients with PUD undergoing upper gastrointestinal endoscopy.Methods: This prospective study was conducted on 150 cases of PUD from August 2009 to February 2011. Endoscopy was done in all cases. Biopsy was done and sent for histopathological examination and rapid urease test for confirmation of presence of H. pylori.Results: Out of 150 patients with mean age of 45.76 years, 109 patients were diagnosed to have been infected with Helicobacter pylori (72.66%). Out of 89 patients with gastric ulcer, 61 patients were infected with Helicobacter pylori (68.53%). Forty two out of 51 patients (82.35%) with duodenal ulcers and 06 of 10 patients (60%) with carcinoma of stomach were positive for H. pylori. The remaining patients were found to be negative for the H. pylori infection.Conclusions: The findings of the study conclude that H. pylori was consistently associated with PUD.

scholarly journals Genotyping and Association of the Presence of Helicobacter Pylori in Dental Plaque and Gastric Biopsy Specimens in Dyspeptic Patients by PCR-RFLP Technique in Southwest of Iran

2021 ◽  
Author(s):  
Mojtaba Moosavian ◽  
Elyas Kushki ◽  
Tahereh Navidifar ◽  
Eskandar Hajiani ◽  
Mahdi Mandegari
Keyword(s):  
Oral Cavity ◽  
Dental Plaque ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Biopsy Specimens ◽  
Urease Test ◽  
Pcr Rflp ◽  
Dental Plaques ◽  
H Pylori ◽  
Southwest Of Iran

Abstract The oral cavity can act as an extra gastric reservoir for H pylori, and also the presence of the bacteria in the oral cavity is associated with a higher risk of dental caries development. The aim of this study was to determine the genotype and evaluate the association of the presence of H. pylori in dental plaque and gastric biopsy specimens in dyspeptic patients in Ahvaz, Southwest of Iran. In this study, 106 patients with recruited dyspeptic complaints were selected and from each patient, two gastric antral biopsy specimens and two dental plagues were examined. The presence of H. pylori was identified by the Rapid Urease Test (RUT) and the amplification of ureAB and 16S rRNA genes. Also, to verify a hypothetical mouth-to-stomach infection route, the enzymatic digestions of three genes of cagA, vacA, and ureAB in H. pylori strains isolated from dental plaques and stomach samples were compared for each same case. H. pylori was found in the stomach of 52.8% (56 /106) and the dental plaques of 17.9% (19/106) of the studied cases. On the other hand, H. pylori was recognized in the stomach of all 19 cases with oral colonization. Following a combination of restriction fragment length polymorphism (RFLP) patterns of these three known genes on stomach and dental plague samples, 14 and 11 unique patterns were seen, respectively. However, for all H. pylori-positive cases (19), the comparison of RLFP patterns of these genes in the dental plaque and gastric biopsy specimens was different for the same case. This study showed, no significant association was observed between the presence of H. pylori in dental plaque and the stomach of the same case.

scholarly journals Genotyping of Helicobacter pylori Virulence Genes cagA and vacA: Regional and National Study

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Rania M. Kishk ◽  
Nashaat M. Soliman ◽  
Maha M. Anani ◽  
Nader Nemr ◽  
Ayman Salem ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Virulence Genes ◽  
Histopathological Examination ◽  
Virulence Gene ◽  
National Study ◽  
Rapid Urease Test ◽  
Urease Test ◽  
Cytotoxin Associated Gene A ◽  
H Pylori

Helicobacter pylori (H. pylori) plays a crucial role in the pathogenesis of gastritis, peptic ulcer, and gastric cancer. The presence of pathogenicity islands (PAI) genes contributes to the pathogenesis of many gastrointestinal disorders. Cytotoxin-associated gene A (cagA) and vacuolating cytotoxin gene (vacA) are the most known virulence genes in H. pylori. So, our aim was to study H. pylori virulence genes’ role in gastric disorders pathogenesis. Our study included 150 adult patients who suffered dyspeptic symptoms and were referred to the GIT endoscopy unit. Gastric biopsies were attained for rapid urease test (RUT) and histopathological examination, and multiplex PCR technique for detection of virulence genes was performed. It was found that 100 specimens were (RUT) positive, of which sixty samples (60%) were PCR positive for H. pylori ureC gene. The vacA and cagA genes were identified in 61.6% and 53% of H. pylori strains, respectively. Only 5 cases were vacA-positive and cagA-negative. The most virulent vacA s1 allele existed in 56.6% of cases. Out of the 60 H. pylori strains, 66% had at least one virulence gene and 34% did not show any virulence gene. H. pylori infection showed significant increase with age. H. pylori are prevalent amid dyspeptic patients in our region. The main genotype combinations were vacA+/cagA+ of s1m1 genotype and they were frequently associated with peptic ulcer diseases, gastritis, and gastroesophageal reflux disease.

scholarly journals Association of Helicobacter Pylori Infection and Stomach Cancer

2015 ◽  
Vol 12 (2) ◽  
pp. 36-41 ◽  
Author(s):  
Narayan Thapa ◽  
Kunda Bikram Shah ◽  
Bharat Bahadur Bhandari ◽  
Bhairab Kumar Hamal ◽  
Amar Shrestha ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Stomach Cancer ◽  
Histopathological Examination ◽  
Helicobacter Pylori Infection ◽  
Rapid Urease Test ◽  
Diffuse Type ◽  
Surgical Department ◽  
Urease Test ◽  
H Pylori ◽  
Carcinoma Stomach

Introduction: Helicobacter pylori (H. Pylori) as a primary etiological factor in carcinoma stomach. Associationof H. Pylori in gastric cancer has been documented to be in more than 50% of cases. In underdevelopedcountries, this association is shown to be much higher according to different studies.Methods: A prospective observational study of 40 consecutive cases of carcinoma stomach was under takenin surgical department of Shree Birendra hospital and Bir hospital, for a period of two years 2009 to 2011.Location and pathological types of the lesion were noted and all specimens were investigated to see presenceof helicobacter pylori by rapid urease test (RUT) and histological examination.Results: Out of 40 patients, helicobacter pylori positivity was seen in 27 (67.5%) cases by both rapid ureasetest and Histopathological examination (HPE). Regarding the pathological types, out of 26 intestinal type of castomach, 20 (76.92%) cases were positive for H. pylori infection, whereas out of 14 diffuse type of ca stomach,7(50%) cases were positive for H. pylori. In 29 cases of distal ca stomach, H. pylori positivity was seen in 22(75.86%) cases, whereas in rest of the 11 cases the lesion involved other part of the stomach, H. pylori positivitywas seen in 5(45.45%) cases.Conclusion: Helicobacter pylori infection is higher in prevalence in cases of stomach cancer. Its associationwith intestinal histological type of stomach cancer is more common than diffuse type. There is higher prevalenceof Helicobacter pylori infection in distal carcinoma. doi: http://dx.doi.org/10.3126/mjsbh.v12i2.12927

scholarly journals A Study of Portal Hypertensive Polyps

2017 ◽  
Vol 4 (1) ◽  
pp. 88
Author(s):  
Aravind C. ◽  
Arul Murugan S. ◽  
Sai Ravi Kiran B.
Keyword(s):  
Portal Hypertension ◽  
Clinical Laboratory ◽  
Histopathological Examination ◽  
Neuroendocrine Tumour ◽  
Gastric Varices ◽  
Rapid Urease Test ◽  
Urease Test ◽  
Vascular Proliferation ◽  
H Pylori ◽  
Hepatic Portal

Background: Portal hypertension induces mucosal changes like portal hypertensive gastropathy, duodenopathy and colonic mucosal abnormalities. These polyps are commonly found in the stomach than other parts of bowel. Portal hypertensive polyp usually occur in cirrhotic portal hypertension and are also in extra hepatic portal vein occlusion.  Diagnostic criteria for portal hypertensive polyp is not yet clearly described. Hence it becomes essential to evaluate the prevalence of polyps in portal hypertensive patients. The aim was to estimate the prevalence of portal hypertensive polyps in patients with portal hypertension due to any etiology.Methods: This is a prospective observational study of patients with portal hypertensive polyps. All patients in age group of 18 to 80 years who had portal hypertension diagnosed by clinical, laboratory and imaging criteria who went upper gastro intestinal endoscopy from the period of May 2012 to April 2013 were included in the study. Diagnosis of portal hypertensive polyps was confirmed histologically. Patients with proton pump inhibitor use, gastric varices,          h. pylori gastritis, history of inherited polyposis syndromes, patients who refused consent were excluded.Results: A total of 3,621upper GI endoscopies were done of which 428 patients had portal hypertension. Polyps were noted in 9 patients (2.1%). Out of 428 patients 366 had chronic liver disease and 62 had extrahepatic portal hypertension and out of them 1 was excluded due to Rapid urease test positivity. Histopathological examination showed four had extensive vascular proliferation and glandular hyperplasia (portal hypertensive polyp) two had hyperplastic polyp and other two had neuroendocrine tumour. Endoscopic appearances of polyps varied considerably, with sizes ranging from 5mm to 15 mm. Polyps were sessile or pedunculated, singular or multiple, found in the antrum, body of the stomach .There is no specific endoscopic features to identify portal hypertensive polyps.Conclusions: Portal hypertensive polyps are rare. There is no specific endoscopic features. Biopsy is necessary for diagnosis. Longterm studies are needed to characterise their significance.

scholarly journals A Comparative Study Among Different Invasive Methods For The Diagnosis Of Helicobacter Pylori

1970 ◽  
Vol 5 (1) ◽  
pp. 21-24
Author(s):  
MDU Islam ◽  
SHZ Rahman ◽  
SM Shamsuzzaman ◽  
N Muazzam ◽  
SG Kibria ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Rapid Urease Test ◽  
World Population ◽  
College Hospital ◽  
Cross Sectional ◽  
Female Ratio ◽  
Medical College ◽  
Diagnostic Endoscopy ◽  
Urease Test ◽  
H Pylori

H. pylori infection occurs worldwide. Approximately 50% of the world population is infected with this organism. A cross-sectional study was conducted on 81 dyspeptic patients attending at Dhaka Medical College Hospital, for diagnostic endoscopy. H. pylori infection was measured by three invasive methods: culture, rapid urease test & histopathology. Among study patients maximum (34.57%) H. pylori infected were in 21-30 years age group, bearing male female ratio 1: 92.86. Forty four (54.32%) out of 81 were culture positive, 61 (75.31%) were rapid urease test positive and 62 (76.54%) were histopathology positive. By using 'gold standard' definition, 64 (79.02%) were H. pylori infected, 17 were un-infected. Finally comparing among three invasive methods, all are highly sensitive and specific to diagnose H. pylori infection. DOI: 10.3329/fmcj.v5i1.6809Faridpur Med. Coll. J. 2010;5(1):21-24

scholarly journals Early Development of Refl ux Esophagitis after Successful Helicobacter Pylori Eradication in Superfi cial Gastritis

2011 ◽  
Vol 51 (184) ◽  
Author(s):  
H H Jeon ◽  
HH Jeon
Keyword(s):  
Helicobacter Pylori ◽  
Los Angeles ◽  
De Novo ◽  
Eradication Therapy ◽  
Rapid Urease Test ◽  
Stomach Body ◽  
Short Period ◽  
Urease Test ◽  
H Pylori

The relationship between gastroesophageal refl ux disease (GERD) and Helicobacter pylori (H. pylori) eradication is still debated. Recently, we had a patient of GERD who had developed it shortly after H. pylori eradication therapy. A 72-year-old man was diagnosed by endoscopy as suffering from severe superfi cial gastritis in the stomach body. A rapid urease test showed H. pylori infection. He was then started on proton pump inhibitor (PPI) based therapy for two weeks eradicating H.pylori. After completion of H. pylori eradication, he complained of a heart-burn sensation. Follow-up endoscopy showed refl ux esophagitis, of grade B according to the Los Angeles classifi cation. Since the patient had developed GERD after completion of the triple therapy, their suggests that H. pylori eradication must have triggered the development of de novo GERD after a short period of time. Keywords: GERD, Helicobacter pylori, PPI.

scholarly journals Helicobacter pylori among the cases of gastritis: a one year study at a tertiary care hospital of South India

2019 ◽  
Vol 6 (12) ◽  
pp. 4294
Author(s):  
Ayathu V. S. Sai Mahesh ◽  
Shyam Sundar Tandri
Keyword(s):  
Helicobacter Pylori ◽  
Tertiary Care Hospital ◽  
Histopathological Examination ◽  
Demographic Data ◽  
Tertiary Care ◽  
Rapid Urease Test ◽  
Care Hospital ◽  
Urease Test ◽  
H Pylori ◽  
One Year

Background: Gastritis is one of the commonest clinical conditions encountered by a physician. The most common cause is said to be infection with Helicobacter pylori. The present study was aimed to diagnose the prevalence of H. pylori infection among the cases of gastritis and its correlation with histopathological findings and associated rapid urease test.Methods: A one year prospective study at a tertiary care hospital was conducted and all cases of gastritis were included and socio demographic data, clinical complaints and duration were noted. Endoscopy was performed on all cases. Biopsy was performed histopathological examination with staining and graded by Houston-updated Sydney system. Rapid urease test was performed and findings noted.Results: 325 cases with 215 male and 110 female cases were included. 26.15% were in age group of more than 60 years and number of cases increased with age. Mean age of study participants was 39.12±2.8 years and heart burn was the common complaint in the study cases. 81.54% of the cases revealed endoscopic gastritis on endoscopy and hyperaemia was commonest. 83.69% were positive by rapid urease test. Rapid urease test is more sensitive than histological staining in confirmation of H. pylori infection.Conclusions: To conclude on the present study, the prevalence of H. pylori infection is on a global rise and appropriate measures to reduce the prevalence is quite an urgent necessity. Histopathological interpretation of gastric biopsies is a reliable indicator of H. pylori infection as well as gastritis grading according to the Sydney grading system.

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