scholarly journals A Comparative Study of Rotaviral Antigen Detection by ELISA and ICT in Children below Five Years with Acute Diarrhoea in A Tertiary Care Hospital

2021 ◽  
Vol 49 (1) ◽  
pp. 14-18
Author(s):  
Farjana Binte Habib ◽  
Mohammed Mirazur Rahman ◽  
Md Moynul Haque ◽  
Shib Prasad Sinha ◽  
Suborna Dey ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Tertiary Care ◽  
Significant Proportion ◽  
Antigen Detection ◽  
Watery Diarrhoea ◽  
Enzyme Linked Immunosorbent Assay ◽  
Care Hospital ◽  
Rotavirus Infection ◽  
Under Five ◽  
Stool Samples

Rotavirus is responsible for acute severe watery diarrhoea in young children. Early and rapid detection of rotavirus infection can help to reduce inappropriate administration of antibiotics and has future positive impact on prevention of drug resistance. This cross-sectional study was designed to determine the role of rotaviral antigen detection by ICT from stool sample of acute diarrhoeal children below five years admitted in Sylhet MAG Osmani Medical College Hospital, Sylhet and was carried out in the department of microbiology in collaboration with the department of paediatrics during the period from 1st January  to 31st December, 2018. Total 184 children of under five years of age with acute watery diarrhoea were enrolled in this study. Rotaviral antigen was detected by ELISA (Enzyme Linked Immunosorbent Assay) and ICT (Immunochromatographic test) from stool samples. Out of 184 stool samples, rotaviral antigen was found positive in 84 and 86 cases by ICT and ELISA methods, respectively. ICT showed sensitivity of 90.70% and specificity of 93.88% when compared with ELISA. The rotavirus infection was found highest in male children (61.90%) and in age group of 7 to 12 months (51.89%). Considering the importance of rotaviral diarrhoea, rapid detection of rotavirus infection by ICT is essentially needed and should be practiced routinely as it is relatively reliable, easy to perform and cost-effective. It is particularly important in Bangladesh, where diarrhoea is still contributing a significant proportion of mortality and morbidity in under five children. Bangladesh Med J. 2020 Jan; 49 (1): 14-18

scholarly journals Prevalence of rotavirus infection and its association with socio-demographic characteristics among under-five children admitted with acute diarrhoea in a tertiary care hospital of Bangladesh

2021 ◽  
Vol 11 (3) ◽  
pp. 186-190
Author(s):  
Farjana Binte Habib ◽  
Mohammed Mirazur Rahman ◽  
Rafaat Choudhury ◽  
Tarun Kanti Paul ◽  
Md Moynul Haque
Keyword(s):  
Tertiary Care ◽  
Acute Diarrhoea ◽  
Watery Diarrhoea ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Morbidity ◽  
Care Hospital ◽  
Cross Sectional ◽  
Rotavirus Infection ◽  
Under Five ◽  
Under Five Children

Background: Rotavirus has been recognized as the most common cause of acute watery diarrhoea among under-five children worldwide. It is considered as a major cause of childhood morbidity and mortality particularly in developing countries like Bangladesh. Considering the high morbidity and significant mortality, this study was designed to evaluate the prevalence of rotaviral diarrhoea among children less than five years in Sylhet region of Bangladesh. Methods: This cross-sectional study was conducted in the Department of Microbiology, Sylhet MAG Osmani Medical College from 1st January to 31st December, 2018. Total 184 under-five children admitted in paediatrics department of the same institute with acute watery diarrhoea were enrolled in this study by convenient sampling. Stool samples were obtained and assayed for rotavirus antigen by sandwich type enzyme linked immunosorbent assay (ELISA). Results: Rotaviral antigen was found positive in 86 cases and disease prevalence was 46.74%. The rotavirus infection was found highest in age group of 7 to 12 months (50.56%) and in male (59.30%) children. Prevalence of infection was associated with age (p= 0.004), socio-economic condition (p=0.001) and exclusive breastfeeding of children (p=0.01). Conclusion: Rotavirus was a significant cause of acute watery diarrhoea and mainly affected children below 12 months age. BIRDEM Med J 2021; 11(3): 186-190

scholarly journals Rotavirus in diarrhoeic children under-five attending a tertiary care hospital in Mumbai, India

2018 ◽  
Vol 5 (5) ◽  
pp. 1863
Author(s):  
Nikhil Khude ◽  
Lona Dash ◽  
Sushma Save ◽  
Badhuli Samal ◽  
Jayanthi Shastri
Keyword(s):  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Acute Diarrhoea ◽  
Enzyme Linked Immunosorbent Assay ◽  
Care Hospital ◽  
Etiological Diagnosis ◽  
Under Five ◽  
Under Five Children ◽  
Stool Samples ◽  
Parasitic Pathogens

Background: Diarrhoeal diseases are a leading cause of morbidity and mortality among children requiring hospitalization in developing countries. Rotavirus is the most common cause of acute diarrhoea among under-five children. Etiological diagnosis of diarrhoea would enable appropriate management of patients while limiting the spread of drug resistant pathogens. This study was undertaken to determine presence of rotavirus and other diarrhoeal pathogens in under-five diarrhoeic children attending a tertiary care hospital, and the related clinical presentations.Methods: 120 stool samples of under-five children with acute diarrhoea, attending the OPD and indoor services of a tertiary care hospital, were studied over one year. Rotavirus antigen was detected using enzyme linked immunosorbent assay. Bacterial and parasitic pathogens were detected using standard microbiological techniques.Results: Out of 120 stool samples tested, 36 were positive for Rota virus antigen. Bacterial isolates included E. coli (25), Vibrio cholerae (12) and Aeromonas species (1). Parasites were observed in nine samples and multiple pathogens in nine.Conclusions: Rotavirus continues to be a major cause of childhood diarrhoea. As antibiotics have no role in the management of viral and parasitic diarrhoeas, etiological diagnosis is imperative for proper management of diarrhoea and prevention of indiscriminate use of antibiotics.

Clinical Profile and Characterization of Rotavirus Strains in Under-Five Children with Diarrhea: A Hospital-Based Study from Eastern Odisha, India

2022 ◽  
Author(s):  
J Bikrant Kumar Prusty ◽  
Jasashree Choudhury ◽  
Goolla Akhila ◽  
Mrutunjay Dash ◽  
Mamata Devi Mohanty ◽  
...  
Keyword(s):  
Tertiary Care ◽  
Clinical Profile ◽  
Categorical Variables ◽  
Care Hospital ◽  
Isolation And Identification ◽  
Surveillance Network ◽  
Rotavirus Infection ◽  
Under Five ◽  
Under Five Children ◽  
Prevalent Strain

Abstract Objective Diarrheal diseases are one of the most common causes of hospitalization in children under five. Rotavirus is the most common cause of acute diarrhea in younger children, and the prevalence decreases rapidly with increasing age. The objective of the study was to estimate the burden of rotavirus infection in acute gastroenteritis among under-five children admitted to a tertiary care hospital in eastern Odisha, for the clinical profile and identity of the prevalent strains. Methods This was a prospective observational study linked to the National Rotavirus Surveillance Network (NRSN), where 720 under-five children with diarrhea were enrolled. In total, 675 stool samples of eligible candidates were sent for rotavirus isolation, and identification of strains was done by identifying VP7 (G-type) and VP4 (P-type) genes by reverse transcription polymerase chain reaction. Results Categorical variables were presented as frequency and percentage, and continuous variables were expressed as mean ± standard deviation. Rotavirus was detected in 256 (37.92%) samples. Males outnumbered females. The most common affected age group was 7 to 12 months, followed by 13 to 18 months. G3P[8] was the most prevalent strain in this study. Conclusion Children between the age of 7 and 18 months were most vulnerable to rotavirus infection. The most prevalent strain varies from one region to another and continuous surveillance is needed.

scholarly journals STUDY OF THREE MALARIA RAPID DETECTION TESTS AND ITS CORRELATION WITH PARASITIC INDEX FOR P.FALCIPARUM AND P.VIVAX

2020 ◽  
pp. 26-29
Author(s):  
Dipti Gaikwad ◽  
Chaya A. Kumar ◽  
Sujata Baveja
Keyword(s):  
Health Care ◽  
Diagnostic Tool ◽  
Rapid Detection ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Antigen Detection ◽  
Care Hospital ◽  
Peripheral Smear ◽  
Malaria Rdts ◽  
Expert Microscopy

Objective: To determine the sensitivity and specificity of three Malaria rapid antigen detection tests(RDTs) .To study the sensitivity of the RDTs in relation to parasitic index . Materials and Methods: The study was conducted at a tertiary care hospital. Peripheral smear were prepared and stained.Parasite index was calculated. Three rapid antigen detection tests ; Optimal – IT, Paramax-3 and QDx malaria PAN/PF were tested. Results: Sensitivity for Optimal – IT was highest (98.47%). QDx malaria PAN/Pf gave highest specificity (97%). All the three RDTs gave sensitivity of 100% at a parasitic index of more than 100 parasites per µl. Sensitivity of Optimal – IT and QDx malaria PAN/Pf for P falciparum and P vivax was 100% and 88.88% respectively at a parasitic index of 51-100 parasites/ µl. Conclusion: Malaria RDTs are a good diagnostic tool in health care set ups where quick results are desired and expert microscopy is not available.

Rapid detection of Rotavirus antigen in stool sample of acute diarrheic children

2012 ◽  
Vol 6 (1) ◽  
pp. 11-13
Author(s):  
Sushmita Roy ◽  
S.M. Shamsuzzaman ◽  
K.Z. Mamun
Keyword(s):  
Stool Sample ◽  
Rapid Detection ◽  
Cross Sectional Study ◽  
Rapid Diagnosis ◽  
Age Group ◽  
College Hospital ◽  
Cross Sectional ◽  
Rotavirus Infection ◽  
Medical College ◽  
Stool Samples

Rotavirus is one of the leading causes of pediatric diarrhea globally. Accurate and rapid diagnosis of Rotavirus diarrhea should reduce unnecessary use of antibiotics and ultimately reduce drug resistance. Study was designed for rapid diagnosis of Rotavirus antigen in stool sample by ICT (Immunochromatographic test) as well as to observe the seasonal variation of rotavirus infection. This cross sectional study was carried out in the department of Microbiology, Dhaka Medical College from January 2011 to December 2011. Eighty stool samples were collected from Dhaka Shishu Hospital and Dhaka Medical College Hospital. All samples were tested for rotavirus antigen by ICT. Among 80 patients, 42 (52.5%) samples were positive for rotavirus antigen. Among these 42 positive samples, 30 (71.43%) were from 0-12 months of age group, 10 (23.81%) from 13 to 24 months of age group and rest 2 (4.76%) from 25 to 36 months of age group. Rotavirus Ag was detected in stool samples from January to April and another peak episode from October to December. Considering the importance of Rotavirus associated diarrhea, rapid detection of Rotavirus infection in human is substantially needed and should be routinely practiced.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19354 Bangladesh J Med Microbiol 2012; 06(01): 11-13

Molecular Detection of Group A Rotavirus in under Five Years Old Children with Acute Diarrhoea Admitted to Yangon Children’s Hospital

2019 ◽  
Vol 31 (1) ◽  
pp. 87-92
Keyword(s):  
Acute Diarrhoea ◽  
Enzyme Linked Immunosorbent Assay ◽  
Viral Gastroenteritis ◽  
Cross Sectional ◽  
Under Five ◽  
Group A Rotavirus ◽  
Rotavirus Vaccination ◽  
Group A ◽  
Stool Samples ◽  
Considerable Morbidity

Rotaviruses are regarded as the most common cause of viral gastroenteritis and are responsible for considerable morbidity and mortality among children especially under five years of age worldwide. In developing countries like Myanmar, where diarrhoea is in the priority childhood disease, rotavirus surveillance and detection of rotavirus genotypes are utmost important. A hospital-based, cross-sectional descriptive study was conducted at Yangon Children‟s Hospital among under five children admitted for acute diarrhoea from January to October 2016. This study includes detection of Group A rotavirus antigen by commercial enzyme-linked immunosorbent assay (ELISA) and genotyping by multiplex RT-PCR. From a total of 488 collected samples, rotavirus antigen was detected in 219 samples (45%). Rotavirus diarrhoea was most common among the age of 6-11 months (38.8%) followed by 12-23 months (37.9%). The results showed that boys were more commonly affected than girls. Detection of rotavirus positivity was peak in February (57.6 %). Out of 219 stool samples with positive ELISA result, 40 stool samples with high optical density value were proceeded for further determination of G and P genotypes. Regarding distribution of G genotypes, the most common G genotype was G9 which comprised 45%, and that of P genotype was P[8] which comprised 92.5%. Regarding combination of G and P genotypes, the most frequent combination is G9P[8], and it constituted 42.5%. Untypable genotypes were seen in 30% of G and 2.5% of P typing. As rotavirus infection can be prevented by vaccine, WHO recommended that rotavirus vaccination should be included in national immunization program especially in countries where prevalence of rotavirus is high. The distribution of G and P genotypes is important in consideration of appropriate vaccine in pre-vaccination and evaluation of effectiveness of vaccine in post-vaccination period. Therefore, the information on currently circulating genotypes of rotavirus in this study will serve as valuable data for vaccination programme.

scholarly journals Prevalence and clinical correlation of hepatitis E virus antibody in the patients’ serum samples from a tertiary care hospital in Thailand during 2015–2018

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Atiporn Boonyai ◽  
Anchalee Thongput ◽  
Thidarat Sisaeng ◽  
Parisut Phumchan ◽  
Navin Horthongkham ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Tertiary Care ◽  
Laboratory Data ◽  
Organ Transplant ◽  
Hospital Setting ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Care Hospital ◽  
Serum Samples ◽  
Igm Antibodies

Abstract Background Prevalence and incidence of hepatitis caused by HEV infection are usually higher in developing countries. This study demonstrated the HEV seroprevalence and incidence of HEV infection in patients with clinical hepatitis in a tertiary hospital in Thailand. Methods A laboratory-based cross-sectional study was conducted using 1106 serum samples from patients suspected of HEV infection sent to the Serology laboratory, Siriraj Hospital, for detecting HEV antibodies during 2015–2018. Prevalence of anti-HEV IgG and IgM antibodies in general patients, including organ transplant recipients and pregnant women in a hospital setting, were determined using indirect enzyme-linked immunosorbent assay (ELISA) kits. Comparison of laboratory data between groups with different HEV serological statuses was performed. Results HEV IgG antibodies were detected in 40.82% of 904 serum samples, while HEV IgM antibodies were detected in 11.75% of 1081 serum samples. Similar IgG and IgM antibody detection rates were found in pregnant women. Interestingly, anti-HEV IgM antibodies were detected in 38.5% of patients who underwent organ transplantation. Patients who tested positive for anti-HEV IgM antibodies had higher alanine aminotransferase levels than those who had not. In contrast, patients who tested positive for anti-HEV IgG had more elevated levels of total bilirubin than those who tested negative. Conclusions HEV seroprevalence and incidence in patients with clinical hepatitis were relatively high in the Thai population, including the pregnancy and organ transplant subgroups. The results potentially benefit the clinicians in decision-making to investigate HEV antibodies and facilitating proper management for patients.

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