Rapid detection of Rotavirus antigen in stool sample of acute diarrheic children

2012 ◽  
Vol 6 (1) ◽  
pp. 11-13
Author(s):  
Sushmita Roy ◽  
S.M. Shamsuzzaman ◽  
K.Z. Mamun
Keyword(s):  
Stool Sample ◽  
Rapid Detection ◽  
Cross Sectional Study ◽  
Rapid Diagnosis ◽  
Age Group ◽  
College Hospital ◽  
Cross Sectional ◽  
Rotavirus Infection ◽  
Medical College ◽  
Stool Samples

Rotavirus is one of the leading causes of pediatric diarrhea globally. Accurate and rapid diagnosis of Rotavirus diarrhea should reduce unnecessary use of antibiotics and ultimately reduce drug resistance. Study was designed for rapid diagnosis of Rotavirus antigen in stool sample by ICT (Immunochromatographic test) as well as to observe the seasonal variation of rotavirus infection. This cross sectional study was carried out in the department of Microbiology, Dhaka Medical College from January 2011 to December 2011. Eighty stool samples were collected from Dhaka Shishu Hospital and Dhaka Medical College Hospital. All samples were tested for rotavirus antigen by ICT. Among 80 patients, 42 (52.5%) samples were positive for rotavirus antigen. Among these 42 positive samples, 30 (71.43%) were from 0-12 months of age group, 10 (23.81%) from 13 to 24 months of age group and rest 2 (4.76%) from 25 to 36 months of age group. Rotavirus Ag was detected in stool samples from January to April and another peak episode from October to December. Considering the importance of Rotavirus associated diarrhea, rapid detection of Rotavirus infection in human is substantially needed and should be routinely practiced.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19354 Bangladesh J Med Microbiol 2012; 06(01): 11-13

scholarly journals Socio Demographic Status of Victims in Vitriolage Cases- A Medico Legal Study

KYAMC Journal ◽  
2017 ◽  
Vol 6 (1) ◽  
pp. 553-556
Author(s):  
Farial Naima Rahman ◽  
Mushtaq Ahmad ◽  
Md Zubaidur Rahman
Keyword(s):  
Cross Sectional Study ◽  
Age Group ◽  
Sectional Study ◽  
Rehabilitation Centre ◽  
Human Right ◽  
College Hospital ◽  
Cross Sectional ◽  
Medical College ◽  
Economic Background ◽  
One Stop

Vitriolage or acid violence is a burning human right violation problem of our country. Over the last few years, acid throwing in Bangladesh was a regular phenomenon where most of the victims were female. This descriptive cross sectional study was carried out from July 2011 to June 2012 among the victims of vitriolage who underwent treatment at One stop Crisis centre (OCC) and burn unit of Dhaka Medical College Hospital and also at the rehabilitation and treatment clinics of acid Survivors Foundation (ASF). Among the victims 92% were female and 25% belonged to 21-30 years age group. Most of the victims were from low socio economic background. Failure in marriage (22%), refusal of indecent proposal for sexual relationship (17%), failure in love affairs (13%) were the main causes behind acid violence. Fifty four percent (54%) had injuries in hand, 51 % (51) had injuries in neck and throat, 43 % (43) in back of body and 41 % (41) in face. The victims of vitriolage suffer in the form of physical, social, mental and economical ways. Rehabilitation centre for the victims, prevention of indiscriminate sale of acids, prompt trial of the accused, creation of acid crime tribunal in each district etc can improve the situation.KYAMC Journal Vol. 6, No.-1, Jul 2015, Page 553-556

scholarly journals Usage of Silver-Stained Polyacrylamide Gels Electrophoresis (PAGE) for Detection of Rotavirus Infection by Direct Identification of Viral Nucleic Acid

2016 ◽  
Vol 15 (2) ◽  
pp. 154-159
Author(s):  
Md Rashedul Kabir ◽  
Rafika Afrose ◽  
ASM Shahidullah ◽  
Md Akram Hossain ◽  
Shyamal Kumar Paul ◽  
...  
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Medical Science ◽  
Cross Sectional Study ◽  
Stool Culture ◽  
Cross Sectional ◽  
Rotavirus Infection ◽  
Medical College ◽  
Stool Samples ◽  
Almost All ◽  
Modified Polyacrylamide

Background: Rotavirus infects almost all children by the age of five. More than 180,000 annual deaths due to rotavirus, occurs in Bangladesh. Aims: This study aimed to determine the incidence of rotavirus infection in children by a modified polyacrylamide gel electrophoresis (PAGE) in stool samples. Materials and Methods: In this descriptive type cross sectional study, a total of 400 stool samples were examined for the presence of rotavirus by a modified PAGE analysis of viral genome. Stool culture was done for common enteric pathogens. The study was carried out from November 2012 to July 2013 in the department of Microbiology, Mymensingh Medical College, Mymensingh. Results: PAGE results were found in 365 of 400 (91%) specimens. Maximum incidence of rotavirus infection was seen in age group of 6 months - 24 months (67.25%). All 151 rotavirus positive cases did not show infection with bacterial pathogens.Conclusion: The modified PAGE technique for the detection of viral RNA was found to be rapid, simple, reliable and less expensive technique.Bangladesh Journal of Medical Science Vol.15(2) 2016 p.154-159

scholarly journals PREVALENCE OF FIBROMYALGIA SYNDROME (FMS) AMONG WOMEN

2021 ◽  
Vol 5 (12) ◽  
Author(s):  
Ashok Meena ◽  
D S Meena ◽  
Narendra Saini ◽  
Poonam Meena
Keyword(s):  
Fibromyalgia Syndrome ◽  
Cross Sectional Study ◽  
Married Women ◽  
Outpatient Department ◽  
Age Group ◽  
College Hospital ◽  
Cross Sectional ◽  
Medical College ◽  
Common Diagnosis ◽  
Made In

Background: The aim of the study is to find the incidence of fibromyalgia in orthopaedic female outpatient department in a medical college hospital Methods: Cross-sectional study conducted on the female patients coming to orthopedic outpatient department. Results: The result showed that about 42.00% patients diagnosed with fibromyalgia syndrome (FMS).  The age group that is majorly affected by fibromyalgia is between 40-60 years of age.  Married women accounts to the major group of 89% Conclusion: We concluded that the most common diagnosis made in an orthopaedic female outpatient department is fibromyalgia Keywords: Fibromyalgia, Orthopaedics, Women

scholarly journals Peak expiratory flow rate in children aged 6 to 14 years

2021 ◽  
Author(s):  
A. Gunasekaran
Keyword(s):  
Primary Outcome ◽  
Reference Value ◽  
Cross Sectional Study ◽  
Outcome Variable ◽  
Age Group ◽  
College Hospital ◽  
Cross Sectional ◽  
Primary Outcome Variable ◽  
Medical College ◽  
Structured Questionnaire

Background: The objective of this study was to record the PEFR values in children aged between 6 to 14 years.Methods: A cross sectional study was conducted among 1205 children aged 6 to 14 years in department of paediatrics at a medical college hospital. In a pre-structured questionnaire, the age, sex and PEFR values were recorded. PEFR was considered as primary outcome variable. SPSS version 20 was used for data analysis.Results: Total of 1205 children, 51% were boys and 49% were girls. Ninety children of age 6 years ranged between 60 to 200 with 111.1±29.4 and 7 years ranging from 70 to 210 with 136.3±30.5. One hundred and five of age 8 years had 156.9±33.3 ranging from 90 to 230 and 126 of age 10 years with 195.7±38.8 ranging from 110 to 290. Mean PEFR for boys increased with their age and was greater than girls.Conclusions: The overall mean PEFR values for the age group 6 to 14 years was 225±90.07 l/min. Boys have higher PEFR value than girls of the same age group. Hence, PEFR values in this study can be used clinically as reference value for children aged 6 to 14 years.

scholarly journals Study of relationship between age and body mass index on knee osteoarthritis in advanced aged females in a divisional city of Bangladesh

2021 ◽  
Author(s):  
Malay K. Roy ◽  
M. Zakir Hossain ◽  
A. H. M. Tanvir Hasan Siddiquee ◽  
M. Alauddin ◽  
Mohammad K. Islam ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Knee Osteoarthritis ◽  
Body Mass ◽  
Sampling Technique ◽  
Cross Sectional Study ◽  
Advanced Age ◽  
Age Group ◽  
College Hospital ◽  
Cross Sectional ◽  
Medical College

<p class="abstract"><strong>Background:</strong> Knee osteoarthritis is a notable issue in senior citizens and is a burning problem worldwide. There are so many risk factors related to knee osteoarthritis but women's sex, age, and obesity are significant. The aim and objective of this study were to estimate the prevalence of knee osteoarthritis among women in the age group of 46 to 65 years and the effect of age and body mass index (BMI) on knee osteoarthritis.</p><p class="abstract"><strong>Methods:</strong> Cross-sectional study was conducted at outpatient department (OPD) in Mymensingh Medical College Hospital, a tertiary level hospital, among females of Mymensingh, a divisional city of Bangladesh in the age group of 46 to 65 years. Purposive sampling technique used to select study subjects. A total of 100 females participated in this study in one month (July 2019-August 2019).<strong></strong></p><p class="abstract"><strong>Results:</strong> 100 participants were enlisted in the study, in the age group of 46 to 55 years 39 (39%) and 56 to 65 years 61 (61%). Total 65 (65%) participants were affected with knee osteoarthritis. Among them, 19 (29.2%) participants were in the age group of 46-55 years and 46 (70.8%) participants were in the age group of 55-65 years, thus in the advanced age group, knee osteoarthritis is remarkably more. Participants affected with knee osteoarthritis with BMI ≥25 were significantly more affected 45 (69.2%) compared with participants with normal BMI 20 (30.2%).</p><p class="abstract"><strong>Conclusions:</strong> Knee osteoarthritis is significantly associated with advanced age and obesity. Advanced age and overweight were strongly associated with osteoarthritis in our population. Therefore, weight reduction and preventive measures can decrease the burden and will help in minimizing morbidity associated with OA.</p>

scholarly journals BUTT AND HIT - THE HEART KIDNEY SAGA: A SNAP SHOT AND LONG TERM PERSPECTIVES

2013 ◽  
Vol 03 (02) ◽  
pp. 051-057
Author(s):  
Raghava Sharma ◽  
Shashank Shetty
Keyword(s):  
Blood Pressure ◽  
Tobacco Smoking ◽  
Tertiary Care ◽  
Young Male ◽  
Cross Sectional Study ◽  
Age Group ◽  
College Hospital ◽  
Cross Sectional ◽  
High Systolic Blood Pressure ◽  
Medical College

AbstractTobacco smoking is an universal problem. There are about 120 million smokers in India in the age group of 30-69 years and have an increased cardiovascular and renal risks in addition to other risks attributable to smoking..The present study was aimed to assess the cardiovascular and renal risks especially among the young smokers in the age group of 20-40 years. After all necessary administrative and ethical clearances a cross sectional study was conducted at the tertiary care medical college hospital located at Mangalore. 75 young male smokers in the age group of 20-40 years along with equal number of age, sex matched non smokers as controls were recruited and each one were assessed for Blood pressure(BP), Electro cardiographic changes(ECG changes), and Glomerular filtration rate(GFR).In the present study Smoking resulted in high systolic blood pressure and prolonged QT interval (QTc) as compared to the non smokers, thus increasing the risk of Sudden cardiac death among smokers. Longer duration of smoking of more than 10 years resulted in Pre hypertension and reduced GFR in comparison to smokers with lesser duration of smoking of less than 10 years.This study highlights the need to tackle “Smoking” among young adults more aggressively to avoid the potential cardiovascular and renal complications, as Tobacco smoking is a major modifiable risk factor for the same.

scholarly journals "Usage of Silver-Stained Polyacrylamide Gels Electrophoresis (PAGE) for Detection of Rotavirus Infection by Direct Identification of Viral Nucleic Acid ."

KYAMC Journal ◽  
2017 ◽  
Vol 6 (1) ◽  
pp. 557-561
Author(s):  
Md Rashedul Kabir ◽  
Md Akram Hossain ◽  
Md Murshed Alam ◽  
Shyamal Kumar Paul ◽  
Zohra Begum ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Stool Culture ◽  
Direct Identification ◽  
Viral Nucleic Acid ◽  
Cross Sectional ◽  
Rotavirus Infection ◽  
Medical College ◽  
Stool Samples ◽  
Almost All ◽  
Modified Polyacrylamide

Background: Rotavirus infects almost all children by the age of five. More than 180,000 annual deaths due to rotavirus, occurs in Bangladesh.Aims: This study aimed to determine the incidence of rotavirus infection in children by a modified polyacrylamide gel elctrophoretic (PAGE) in stool samples.Materials and Methods: In this descriptive type cross sectional study, a total of 400 stool samples were examined for the presence of rotavirus by a modified PAGE analysis of viral genome. Stool culture was done for common enteric pathogens. The study was carried out from November 2012 to July 2013 in the department of Microbiology, Mymensingh Medical College, Mymensingh.Results: PAGE results were found in 365 of 400 (91%) specimens. Maximum incidence of rotavirus infection was seen in age group of 6 months - 24 months (67.25%). All 151 rotavirus positive cases did not show infection with bacterial pathogens.Conclusion: The modified PAGE technique for the detection of viral RNA was found to be rapid, simple, reliable and less expensive technique.KYAMC Journal Vol. 6, No.-1, Jul 2015, Page 557-561

scholarly journals Organophosphorus Compound Poisoning and Its Outcome: Experience from a Teaching Hospital of Bangladeh

2017 ◽  
Vol 6 (2) ◽  
pp. 74-78
Author(s):  
Md Daharul Islam ◽  
Khaleda Akter ◽  
Md Azizul Hoque ◽  
ARM Saifuddin Ekram
Keyword(s):  
Organophosphorus Compound ◽  
Time Lapse ◽  
Cross Sectional Study ◽  
Agricultural Products ◽  
Age Group ◽  
Sectional Study ◽  
College Hospital ◽  
Cross Sectional ◽  
Medical College ◽  
The World

Background: Household and agricultural products containing organophosphorus compound (OPC) pesticides are easily available allowing opportunities for easy source of poisoning throughout the world. Bangladesh is no exception. This study was aimed to find out some epidemiological and demographic profile and immediate outcome of OPC poisoning cases.Methods: This cross-sectional study was done at Rajshahi Medical College Hospital from January to December 2007 and consecutively selected 100 patients with OPC poisoning were studied.Results: Half of the patients belonged to age group 16 25 years, 60% were male, 60% were married, 36% were illiterate and 25% were housewives. Suicidal attempt (85%) was the main intention and the commonest cause was familial disharmony (42%). Gastrointestinal (91%) and pupillary (90%) features were predominant. Most (65%) cases received initial treatment at local hospitals within 30 minutes of ingestion of poison and severity was mild. Forty percent patients required less than 50 ampules of atropine and 75% required 0 3 ampules of pralidoxime. Full recovery was achieved in 82% cases, 8% developed intermediate syndrome and 10% died (6/10, 60% within 24 hours of admission). Hospital stay was <5 days in 83.3% casesConclusion: OPC poisoning severity and time lapse between OPC poisoning and hospitalization is an important factor in determining outcome of OPC poisoning.Birdem Med J 2016; 6(2): 74-78

Suicidal Death Autopsy Analysis at Dhaka Medical College

1970 ◽  
Vol 40 (1) ◽  
pp. 18-21 ◽  
Author(s):  
MN Hossain ◽  
Z Rahman ◽  
S Akhter
Keyword(s):  
Forensic Medicine ◽  
Causes Of Death ◽  
Cross Sectional Study ◽  
Age Group ◽  
Sectional Study ◽  
Cross Sectional ◽  
Medical College ◽  
Autopsy Report ◽  
Common Causes ◽  
Suicidal Death

A cross sectional study was carried out at the department of Forensic Medicine in Dhaka Medical College during the period of January 2008 to December 2009. Data were collected from 3rd copy of the post mortem reports which were preserved in the department of Forensic Medicine with the verbal consent of the doctors who performed autopsy report. During this period total 5114 autopsies were conducted. Out of this 970 cases (19%) were suicidal in nature. It was noticed that all suicidal deaths occurred from 10 years to all age group respectively, but top amongst age group of suicidal deaths occurred in between 21 to 30 years of people. Suicidal deaths are more common in female than male. Suicidal deaths due to hanging is highest, next common causes of death due to organophosphorus compund poisoning. Suicidal deaths by hanging is more in female than male but in poisoning cases male are more lvictimised than female. Objectives of our study are to see the occurrence and methods of suicidal death. DOI: http://dx.doi.org/10.3329/bmj.v40i1.9957 BMJ 2011; 40(1): 18-21

scholarly journals Identification of Hookworm Species in Stool By Harada Mori Culture

2012 ◽  
Vol 4 (2) ◽  
pp. 3-4 ◽  
Author(s):  
Sazzad Bin Shahid ◽  
A Chowdhury ◽  
S M Shamsuzzaman ◽  
K Z Mamun
Keyword(s):  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Laboratory Methods ◽  
Necator Americanus ◽  
Medical College ◽  
Stool Samples ◽  
Hookworm Infections

This cross sectional study was done from January 2009 to June 2010 in Microbiology department of Dhaka Medical College, Dhaka to identify hookworm species and to compare different laboratory methods for diagnosis of hookworm infections. Among the 375 stool samples evaluated, 26 (6.93%) samples were positive for hookworms. Ankylostoma duodenale were 3 (11.50%) and Necator americanus were 23 (88.50%). Harada Mori culture was found as the most effective method for detection of hookworms.DOI: http://dx.doi.org/10.3329/bjmm.v4i2.10821 

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