scholarly journals Antidiabetic Activity of Lupeol and Lupeol Esters in Streptozotocin- Induced Diabetic Rats

2015 ◽  
Vol 17 (2) ◽  
pp. 138-146 ◽  
Author(s):  
Vijai Lakshmi ◽  
Abbas Ali Mahdi ◽  
Mohammad Kaleem Ahmad ◽  
Santosh Kumar Agarwal ◽  
Arvind Kumar Srivastava
Keyword(s):  
Ethanol Extract ◽  
Pharmaceutical Journal ◽  
Chemotherapeutic Agents ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Long Chain Fatty Acids ◽  
Acid Chlorides ◽  
Pharmacological Activities ◽  
One Step ◽  
Derivatives Of

Currently, natural products have been shown to present interesting biological and pharmacological activities and are also used as chemotherapeutic agents. Plants have been used in treating cancer, cardiovascular disorders, tuberculosis and many other diseases. Therefore, the current study was designed to evaluate the antidiabetic potential of lupeol and its semisynthetic derivatives to get a new and potent antidiabetic agent. The ethanol extract of Crataeva nurvala, its hexane and chloroform soluble fractions and lupeol isolated from the extractives were evaluated.. Lupeol derivatives were prepared through a one-step reaction with acid chlorides, long chain fatty acids and aromatic moieties. A series of ester derivatives of lupeol were assayed for antidiabetic activity in (STZsucrose model rats. Few derivatives of lupeol showed more potent activity as compared to the basic molecule, lupeol. The results of the present study clearly indicated that the ethanol extract, fractions and lupeol isolated from C. nurvala and synthetic lupeol analogs possess significant antidiabetic activity. The models used for studying theantidiabetic activities have already been validated. In our studies, it was found that the ester derivatives of lupeol posses better antidiabetic potential when compared to lupeol. It is thus concluded that lupeol skeleton deserves further investigation for the development of more potent and non-toxic new antidiabetic agents for therapeutic applications. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22330 Bangladesh Pharmaceutical Journal 17(2): 138-146, 2014

scholarly journals Antidiabetic and anti-obesity acylated flavonol diglucoside from Ammannia baccifera L. subsp. aegyptiaca (Willd.) Koehne Waste

2020 ◽  
Author(s):  
Noha Swilam ◽  
Mahmoud Nawwar ◽  
Rasha Radwan ◽  
Eman Mostafa
Keyword(s):  
Binding Free Energy ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Insulin Level ◽  
Diabetic Rats ◽  
High Serum ◽  
Antidiabetic Activity ◽  
Active Site Residues ◽  
Sod Activity ◽  
Aerial Parts

Abstract Chemical investigation of the aerial parts of Ammania aegyptiaca ethanol extract (AEEE) revealed significant high concentrations of polyphenols and flavonoids content with notable antioxidant activity in DPPH, ORAC, and reducing power assay. New acylated diglucoside flavonol myricetin 3-O-β-4­C1-(6"-O-galloyl glucopyranoside) 7-O-β-4C1-glucopyranoside (MGGG) was isolated from aerial parts of AEEE along with four additional known phenolics, not characterized previously from AEEE. Moreover, powerful inhibitory effects of MGGG, AEEE, and all isolates against α-amylase, pancreatic lipase and β-glucosidase, were assessed. In addition, flexible molecular docking was used to reveal the inhibition towards digestive enzymes and confirmed that the MGGG interacted strongly with the active site residues of these enzymes with the highest binding free energy against β-glucosidase (DG=-8.98 kcal/mol) compared to the commercial drug Acarbose, thus justifying its dual management of diabetes and obesity. In streptozotocin (STZ) induced diabetic rats, AEEE significantly decreased high serum glucose, α-amylase activity, liver and kidney function markers and increased insulin level. Moreover, it improved lipid profile due to diabetes with increased SOD activity and inhibited of TBARS formation. Consequently, AEEE and MGGG are found useful in controlling the secondary complications associated with type 2 diabetes mellitus. Histopathological studies proved the decrease in the pancreas damage and agreed with the biochemical findings. These results provide evidence that AEEE and MGGG have potent antidiabetic activity, which warrants additional investigations.

scholarly journals Comparative Study of Antidiabetic Activity of Hexane-Extract of Lemon Peel (Limon citrus) and Glimepiride in Alloxan-Induced Diabetic Rats

2012 ◽  
Vol 15 (2) ◽  
pp. 131-134 ◽  
Author(s):  
Mohammad Naim ◽  
Farhad Mohammad Amjad ◽  
Sania Sultana ◽  
Sheikh Nazrul Islam ◽  
Muhammad Amjad Hossain ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Metabolic Disorder ◽  
Elevated Level ◽  
Pharmaceutical Journal ◽  
Diabetic Rats ◽  
Hexane Extract ◽  
Antidiabetic Activity ◽  
Citrus Limon ◽  
Lemon Peel

Diabetes is classified as a metabolic disorder manifested by elevated level of glucose in the blood. An attempt has been made to compare the antidiabetic activity of hexane extract of lemon peel (Citrus Limon) with that of a marketed product, glimepiride, in alloxan-induced diabetic rats. The study was performed by the measurement of blood glucose level using a glucometer. It was found that the hexane extract of lemon peel showed antidiabetic activity comparable to that of glimepiride. It can thus be inferred that the extract of lemon peel possesses significant antidiabetic activity. DOI: http://dx.doi.org/10.3329/bpj.v15i2.12577 Bangladesh Pharmaceutical Journal 15(2): 131-134, 2012

scholarly journals Evaluation the Effect of Vernonia amygdalina. Del Leaves Ethanol Extract on Blood Sugar and its Synergism with Glibenclamide in Rats

2020 ◽  
Vol 13 (1) ◽  
pp. 26-34
Author(s):  
Igwe K ◽  
Ikpeazu O ◽  
Otuokere I
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Vernonia Amygdalina ◽  
Treatment Groups ◽  
Glucose Levels

Antidiabetic activity of Vernonia amygdalina and its possible synergism with glibenclamide was checked. Forty eight rats were used for the research, for hypoglycermic study of V. amygdalina alone, they were grouped into five of six rats each. Group 1 was the negative control and was administered distilled water orally. Groups 2, 3, and 4 were the treatment groups which received 100, 200 and 300 mg/kg body weight of the V. amygdalina extract respectively orally by intubation. Group 5 was the positive control group which received a known antidiabetic drug, glibenclamide. Diabetes was induced with alloxan. For the synergism study, another 18 rats grouped into 3 of six rats each was used. Both groups of glibenclamide only and glibenclamide plus V. amygdalina extract were dosed for 14 days orally by intubation, thereafter were sacrificed and blood collected from heart for analysis. There were 5 replicates grouped by weight throughout the study and both single and synergistic studies had the same controls. Effect of V. amygdalina extract was checked on blood glucose and its possible synergism with glibenclamide. All results in treatment groups were compared with the normal control at statistical confidence of p<0.05. Result shows that V. amygdalina extract reduced blood glucose level in the test groups as dose of extract increased. Combination of V. amygdalina with glibenclamide demonstrated further deduction in blood glucose levels in the treatment rats groups. Therefore addition of V. amygdalina into glibenclamide increased efficacy in the diabetic rats. The interaction between V. amygdalina and glibenclamide in this work was additive and therefore synergistic.

scholarly journals Antidiabetic activity of musa acuminata colla fruit peel (MACFP) ethanol extract in glucose-induced diabetic rats

2018 ◽  
Vol 197 ◽  
pp. 07007
Author(s):  
Erza Genatrika ◽  
Visda Nila Happy Laksari ◽  
Tjiptasurasa Tjiptasurasa
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diabetic Rats ◽  
Musa Acuminata ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Fruit Peel

Diabetes mellitus is a metabolic disorder disease characterized by increased blood glucose levels due to impaired insulin secretion or increased insulin resistance. The study was performed to investigate the blood glucose lowering effect of Musa acuminata Colla fruit peel (MACFP) ethanol extract in experimentally induced diabetic rats. 25 rats were divided into five groups, i.e negative control group (0.5% NaCMC), positive control group (glibenclamide), and treatment group of MACFP ethanol extract with dose 250, 375, and 500 mg/kg body weight (mg/kg BW). The blood sample was taken from the lateral vein of the tail, and then blood glucose level was observed on 0, 30, 60, 90, and 120 minutes. The results provide information that MACFP ethanol extract with dose 250, 375, and 500 mg/kg BW have the same effect with a positive control (glibenclamide) in lowering blood glucose level on diabetic rats (p>0,05). MACFP ethanol extract with dose 500 mg/kg BW had the highest percentage of decrease in blood glucose level (42,62%), followed by doses 375 mg/kg BW (37,26%) and 250 mg/kg BW (24,12%).

scholarly journals ANTIDIABETIC ACTIVITY OF BACOLEPIS NERVOSA (WIGHT AND ARN.) DECNE. EX MOQ. EXTRACT ON ALLOXAN INDUCED DIABETIC RATS

2016 ◽  
Vol 8 (11) ◽  
pp. 320
Author(s):  
Arockia Jenecius Alphonse A. ◽  
Mohan V. R. ◽  
Doss A.
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Serum Enzymes ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Ethanol Extracts ◽  
Wistar Albino Rats ◽  
Alloxan Monohydrate ◽  
Lipid Parameters

Objective: The aim of this study was to investigate the effect of ethanol extracts of stem and leaf of Bacolepis nervosa as antihyperglycemic, anti-hyperlipidemic and antioxidant activity in alloxan-induced diabetic rats. Methods: Diabetes was induced in wistar albino rats by administration of alloxan monohydrate (150 mg/kg). The ethanol extract of B. nervosa leaf and stem at a dose of 150 and 300 mg/kg body weight was administrated at a single dose per day to diabetes-induced rats for a period of 14 d. The effect of ethanol extract of B. nervosa leaf and stem on blood glucose, insulin, urea, creatinine, HbA1C, serum protein, albumin, globulin, serum enzymes, serum lipid profiles, lipid peroxidase (LPO) and antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) were measured in the diabetic rats.Results: The ethanol extract of B. nervosa stem and leaf elicited significant reduction in blood glucose (p<0.001), serum enzymes (SGPT, SGOT, ALP) (p<0.01), lipid parameters (TC, TG, VLDL-LDL, PL) (p<0.01) except HDL-C and significantly increased insulin (p<0.01), HDL-C (p<0.05),, GPx, GSH, SOD and CAT (p<0.05) at the dose of 300 mg/kg when compared with the diabetic-induced control.Conclusion: From the above results, it is concluded that ethanol extracts of B. nervosa leaf and stem possesses significant antihyperglycemic, antihyperlipidemic and antioxidant effect in alloxan induced diabetic rats.

Acute toxicity and antidiabetic activity of Asystacia gangetica leaf ethanol extract

2019 ◽  
Vol 50 (1) ◽  
pp. 179-196 ◽  
Author(s):  
Eunice Ngozi Ezembu ◽  
Chioke Amaefuna Okolo ◽  
James Obiegbuna ◽  
Florence Chika Ikeogu
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Acute Toxicity ◽  
Glucose Level ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Content Type ◽  
Acute Toxicity Study ◽  
Different Doses

Purpose The purpose of this study is to examine the acute toxicity and antidiabetic activity of Asystacia gangetica leaf ethanol extract. Design/methodology/approach The study was designed as completely randomized in vivo experimental model. Where acute toxicity study was carried out using 30 albino mice, randomly assigned into six groups of five mice each. Toxicity signs and mortality were observed in the rats within a period of 24 h. The acute and sub-acute antidiabetic study was carried out using 50 rats, randomly assigned into five groups of 10 rats each. The rats’ blood glucose levels were determined and used to assess the acute and sub-acute antidiabetic activity of the extract. Findings Results obtained from the acute toxicity study indicated no death in any of the study groups, even at 5,000 mg/kg body weight and showed no signs of toxicity. The acute antidiabetic study showed that treatment with 400 mg/kg of the extract significantly (p = 0.01) lowered glucose level in the diabetic rats from 430.6 to 177.4 mg/dl while 800 mg/kg brought down glucose level from 370 to 144.2 mg/dl by the end of 6 h following administration when compared with the diabetic control group. It was observed that the effect of the extract mostly at 800 mg/kg also compared favorably with that of the standard drug (glibenclamide), which lowered glucose level in diabetic rats from 374.2 to 176.4 mg/dl. Furthermore, the significant reduction was evident from 4, 2 and 2 h for 400 mg/kg extract, 800 mg/kg extract and 5 mg/kg glibenclamide, respectively. At sub-acute level the blood glucose was lowered from 155.6 to 127.2 mg/dl, 137 to 124.4 mg/dl and 151.8 to 121.8 mg/dl for diabetic rats treated with 400 mg/kg, 800 mg/kg and 5 mg/kg glibenclamide, respectively, when compared to the diabetic untreated rats, which ranged from 417.6 to 358.6 mg/dl. The biochemical profile, lipid profile and hematological examination were all positively restored near to normal with the herbal treatment at the different doses. At histopathology level, the liver of the rats treated with 400 mg/kg had moderate portal inflammation without interface or lobular hepatitis while that of 800 mg/kg showed severe portal inflammation with the interface and lobular hepatitis with extensive confluents necrosis. The pancreatic cells of the treated rat showed no significant difference in the β-cells of the islets of Langerhans with hyperplasia of the acinar cell when compared to the diabetic untreated. Research limitations/implications The record of no death and signs of toxicity implies that the extract is safe for consumption even at a high dosage of 5,000 mg/kg body weight. The significant (p = 0.01) reduction in the plasma glucose level of the treated rats as compared to the control is an indication of blood glucose-lowering potential of the extract at two different doses. The significant reduction evident of the extract at different hours and days for the two doses implies that the extract rate of lowering potentials is dose-dependent. The evidence of moderate-severe portal inflammation with the interface and lobular hepatitis at 800 mg/kg treatment is an indication that the intake of this herb at high dosage for long period is not safe for the liver tissue. Practical implications The outcome of this study suggested that the Asystacia gangetica should also be used as a vegetable in-home food preparation and food processing to use its antidiabetic effect. The herbal extract could also be incorporated into a food product and processed into herbal tea bag for convenient. The subjection of this herbal plant to heat treatment during processing could be a possible avenue to make it safe. Social implications The economic burden and complications of diabetes mellitus management will be reduced if the practical implication of this research finding is implemented in foods as vegetable and in functional food production. Originality/value This study revealed that Asystacia gangetica leaf extract may be safe and effective for use at a low dose for acute management of diabetes mellitus. This research may be of value to those living with diabetes mellitus.

scholarly journals EFFECT OF EXACUM WIGHTIANUM ARN., AN ENDEMIC MEDICINAL PLANT ON THE ANTI DIABETIC ACTIVITY

2015 ◽  
Vol 2 (2) ◽  
pp. 96-99
Author(s):  
Baluprakash T ◽  
Arumugasamy K ◽  
Shalimol A ◽  
Nanthakumar R ◽  
Abdul kaffoor H
Keyword(s):  
Ethanol Extract ◽  
Liver Weight ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Pilot Studies ◽  
Glucose Levels ◽  
Control Of Diabetes ◽  
Acute Study ◽  
Endemic Medicinal Plant

The study revealed the antidiabetic activity of the ethanol extract of Exacum wightianum Arn. (Gentianaceae) in streptozotocin induced diabetic rats. The pilot studies were carried after oral administration at doses of 100, 200, 500 and 1000 mg/kg b.wt. in sub-acute study. In diabetic induced rats fed with the extract at 100 and 200 mg/kg body b.wt. The fasting plasma glucose levels were reduced to near normal body and liver weight were found to be increased. Whereas blood glucose, protein, albumin and creatinine levels were estimated after two weeks. The extract significantly inhibited the induction of albuminuria, proteinemia and uremia. The present study clearly indicated a significant antidiabetic activity with the ethanol extract of E. wightianum supports the traditional usage of the plant by Ayurvedic physicians for the control of diabetes. The extract could be useful in preventing the incidence of long term complications of diabetes mellitus.

scholarly journals Evaluation of Anti-Hyperglycaemic activity of Ethanolic Extract of Stems of Bryophyllum pinnatum against Type 2 Diabetic rats

2021 ◽  
Vol 12 (2) ◽  
pp. 309-313
Author(s):  
Pandian P ◽  
Vijay A ◽  
Kathiresan K
Keyword(s):  
Low Dose ◽  
Density Lipoprotein ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Vehicle Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Bryophyllum Pinnatum

To evaluate antidiabetic activity of stems of Bryophyllum pinnatum in Alloxan male Wistar rats cause diabetes. Thirty albino rats were divided into distinct five groups (n=6). By Intraperitoneal injection caused the onset of diabetes of Alloxan (120mg/Kg). vehicle control, negative control, 200, and 400mg/kg ethanolic extracts were given orally in 28 days as single administrations to the vehicle control, diabetic control, normal group, and test groups.  Glucose, (fasting and post prandial for 1st and 21st day) serum cholesterol, Triglyceride, HDL (High density lipoprotein), LDL (Low density lipoprotein) Serum creatinine and urea were estimated with blood tests. The student's t test was performed to evaluate the results. In diabetic rats, a low dose of ethanol extract 200mg/kg significantly decreased all biochemical markers of diabetes. A 400mg/kg of ethanol extract was given to diabetic rats and showed substantial decreases in blood glucose and biochemical parameters. Both ethanol high and low dose extracts show antidiabetic activity in Alloxan induced diabetes.

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