scholarly journals Evaluation the Effect of Vernonia amygdalina. Del Leaves Ethanol Extract on Blood Sugar and its Synergism with Glibenclamide in Rats

2020 ◽  
Vol 13 (1) ◽  
pp. 26-34
Author(s):  
Igwe K ◽  
Ikpeazu O ◽  
Otuokere I
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Vernonia Amygdalina ◽  
Treatment Groups ◽  
Glucose Levels

Antidiabetic activity of Vernonia amygdalina and its possible synergism with glibenclamide was checked. Forty eight rats were used for the research, for hypoglycermic study of V. amygdalina alone, they were grouped into five of six rats each. Group 1 was the negative control and was administered distilled water orally. Groups 2, 3, and 4 were the treatment groups which received 100, 200 and 300 mg/kg body weight of the V. amygdalina extract respectively orally by intubation. Group 5 was the positive control group which received a known antidiabetic drug, glibenclamide. Diabetes was induced with alloxan. For the synergism study, another 18 rats grouped into 3 of six rats each was used. Both groups of glibenclamide only and glibenclamide plus V. amygdalina extract were dosed for 14 days orally by intubation, thereafter were sacrificed and blood collected from heart for analysis. There were 5 replicates grouped by weight throughout the study and both single and synergistic studies had the same controls. Effect of V. amygdalina extract was checked on blood glucose and its possible synergism with glibenclamide. All results in treatment groups were compared with the normal control at statistical confidence of p<0.05. Result shows that V. amygdalina extract reduced blood glucose level in the test groups as dose of extract increased. Combination of V. amygdalina with glibenclamide demonstrated further deduction in blood glucose levels in the treatment rats groups. Therefore addition of V. amygdalina into glibenclamide increased efficacy in the diabetic rats. The interaction between V. amygdalina and glibenclamide in this work was additive and therefore synergistic.

scholarly journals NANOEMULSION OF OKRA FRUIT EXTRACT AS ANTIDIABETIC TREATMENT

2020 ◽  
pp. 138-142
Author(s):  
RATNA DJAMIL ◽  
SARAH ZAIDAN ◽  
DENI RAHMAT ◽  
DIAH KARTIKA PRATAMI ◽  
FELIX HAKIM
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Glucose Levels ◽  
Antidiabetic Treatment ◽  
Blood Glucose Levels ◽  
Average Blood Glucose

Objective: Okra (Abelmoschus esculentus (L.) Moench) has potential antidiabetic activity. This study created a nanoemulsion of okra extract (NOE) and examined its activity on alloxan-induced diabetes mellitus in mice. Methods: Okra was macerated with 70% ethanol and dried in a rotary evaporator into the crude extract. The extract was encapsulated in a solution of glyceryl caprylate, propylene glycol, and glycerine to form a nanoemulsion. To determine the antihyperglycaemic effect of okra extract, 35 male mice (Mus musculus L.) were divided into seven groups: a non-diabetic normal control group and six diabetic mice groups (untreated negative control, glibenclamide-treated positive control, and four treatments with okra ethanol extract (OEE) at 200 and 400 mg/kg BW and NOE at 200 and 400 mg/kg BW). Results: The group treated with NOE at 400 mg/kg BW (NOE400) had the lowest average blood glucose level of 93.4 mg/dL among hyperglycaemic mice. The decrease in blood glucose levels in NOE400 (52.05%) was significantly different from those in the positive control (42.63%) and OEE treatments (39.32%). The nanoemulsion used in this study fulfills quality requirements, with a mean particle size of 134.7 nm, a polydispersity index of 0.512, and a zeta value of −26.72 mV. Conclusion: NOE reduced blood glucose levels in alloxan-induced hyperglycaemic mice better than OEE. Nanoemulsion can improve the antidiabetic activity of okra extract by increasing penetration of active compounds into interstitial space so that their delivery and bioavailability are higher.

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

scholarly journals Antidiabetic activity of musa acuminata colla fruit peel (MACFP) ethanol extract in glucose-induced diabetic rats

2018 ◽  
Vol 197 ◽  
pp. 07007
Author(s):  
Erza Genatrika ◽  
Visda Nila Happy Laksari ◽  
Tjiptasurasa Tjiptasurasa
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diabetic Rats ◽  
Musa Acuminata ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Fruit Peel

Diabetes mellitus is a metabolic disorder disease characterized by increased blood glucose levels due to impaired insulin secretion or increased insulin resistance. The study was performed to investigate the blood glucose lowering effect of Musa acuminata Colla fruit peel (MACFP) ethanol extract in experimentally induced diabetic rats. 25 rats were divided into five groups, i.e negative control group (0.5% NaCMC), positive control group (glibenclamide), and treatment group of MACFP ethanol extract with dose 250, 375, and 500 mg/kg body weight (mg/kg BW). The blood sample was taken from the lateral vein of the tail, and then blood glucose level was observed on 0, 30, 60, 90, and 120 minutes. The results provide information that MACFP ethanol extract with dose 250, 375, and 500 mg/kg BW have the same effect with a positive control (glibenclamide) in lowering blood glucose level on diabetic rats (p>0,05). MACFP ethanol extract with dose 500 mg/kg BW had the highest percentage of decrease in blood glucose level (42,62%), followed by doses 375 mg/kg BW (37,26%) and 250 mg/kg BW (24,12%).

scholarly journals UJI AKTIVITAS ANTIDIABETES EKSTRAK ETANOL BUAH CABAI MERAH ( Capsicum annuum L. ) TERHADAP MENCIT PUTIH JANTAN

2019 ◽  
Vol 9 (1) ◽  
pp. 86 ◽  
Author(s):  
Zulkarni Zulkarni
Keyword(s):  
Blood Glucose ◽  
Capsicum Annuum ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Capsicum Annuum L ◽  
Blood Glucose Levels ◽  
Significant Difference

This Research was conducted to determine the effect of ethanol extract from red chilli (Capsicum annuum L)in lowering blood glucose levels of hyperglycemic male white mices. This study used 30 malewhitemices and divided into 6 groups: negative control group, the positive control group, the treatmentgroup withdosage of 200 mg / kgweight, 400 mg / kgwieght, 600 mg / kg weight and a comparison group with glibenclamide with dosage of 5 mg / kgweightadministered orally for 21 days. The level of fasting blood glucose was checked 6 days after dexamethasone induced, and after the ethanol extract of red chilies on day 7th, 14th, and 21st. The data was analyzed statistically with one –way and two-way Anova by usingSPSS16 program and proceed with the test Duncan to look the significant difference between treatments. The results showed that the ethanol extract from red chilies with a dosage of 200mg / kgweight, 400mg / kgweight, 600 mg / kgweight showed the effect in lowering blood sugar levels in male white mices hyperglycemia significantly (p <0.05). The duration ethanol extract of red chili affected blood glucose levels. The most effective duration in lowering blood glucose levels is the administration of a preparation within 21 days.

The Effect of Ethanol Extract of Guava Leaf on Hypercholesterolemia-Diabetes of White Rats Male Induced by High Fat Feed and Streptozotocin

2021 ◽  
Vol 7 (2) ◽  
pp. 177-187
Author(s):  
Joni Tandi ◽  
Franciska Diana Pratiwi ◽  
Surisna Surisna ◽  
Niluh Puspita Dewi ◽  
Magfirah Magfirah
Keyword(s):  
Blood Glucose ◽  
Effective Dose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Group I ◽  
Guava Leaf

This study aims to determine the effect of guava leaf extract (Psidiium guajava L) on hypercholesterolemia-diabetes and the effective dose for reducing cholesterol and blood glucose levels. This study is a laboratory experimental study using 30 rats divided into 6 test animals. the treatment group, each group consisted of 5 rats. Group I was normal control, group II negative control was given Na-CMC suspension, group III positive control was given simvastatin group IV dose 150 mg / kg BW, group V dose 250 mg / kg BW and group VI dose 350 mg / kg BW. The results showed that the ethanol extract of guava leaves had an effect on reducing blood glucose levels of male white rats with an effective dose of 250mg / kg BW with an average reduction of 119 mg/dL and effectively reduce cholesterol levels at a dose of 150 mg /kg BW with an average reduction of 28,33 mg/dL.

scholarly journals ANTIDIABETIC AND ANTIHYPERLIPIDAEMIC EFFECTS OF THE ETHANOL EXTRACT OF THE LEAVES AND STEM OF CISSUS GRACILLIS (GULL ET PERR) (VITACEAE)

2021 ◽  
pp. 54-56
Author(s):  
OLUSAYO A SHORINWA ◽  
GORDON EI EMENU
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Phytochemical Screening ◽  
Glucose Levels ◽  
Diabetic Control Group ◽  
Alloxan Monohydrate

Objectives: This study investigated the antidiabetic and antihyperlipidemic potential of the ethanol extract of the leaves and stem of Cissus gracillis on alloxan monohydrate-induced diabetic albino rats. Methods: Preliminary phytochemical screening and acute toxicity were carried out. Animals were assigned into seven groups of five rats each. Groups A and B were administered 10 mg/kg each of glibenclamide and atorvastatin respectively, C, D, and E were given 125, 250 and 500 mg/kg of ethanol extract of C. gracillis, respectively, daily for 21 days through oral gavage, group F was diabetic but untreated (diabetic control group), while group G was non-diabetic and untreated which served as the control group. Results: Phytochemical screening revealed the presence of steroids/triterpenoids and carbohydrates. LD50 was above 5000 mg/kg. The extract at 500 mg/kg showed a statistically significant (p<0.05) decrease in blood glucose level when compared with the glibenclamide group on day 21. However, gradual non- significant reduction in blood glucose levels were observed in the extract treated groups on the 7th, 14th, and 21st days of treatment. The administration of ethanol extract of C. gracillis to alloxan-induced diabetic rats produced a decrease in total cholesterol, triglycerides, and low-density lipoproteins comparable to glibenclamide and atorvastatin. Conclusion: The ethanol extract of the leaves and stem of C. gracillis possess a mildly significant antidiabetic and antihyperlipidemic activity.

scholarly journals Effects of goat milk kefir fortified with vitamin D3 on Interleukin-18 levels in diabetic rats

Food Research ◽  
2020 ◽  
Vol 4 (S3) ◽  
pp. 197-201
Author(s):  
K.D. Anggraeni ◽  
G. Anjani ◽  
M. Ardiaria ◽  
C. Nissa ◽  
S.Y. Huang ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Vitamin D3 ◽  
Fasting Blood Glucose ◽  
Goat Milk ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Control Group Design

Hyperglycemia causes increased oxidative stress through an imbalance of reactive oxygen species and antioxidative mechanisms. It stimulates the production of inflammatory mediators and cytokines such as TNF-α, interleukin (IL)-1, and IL-18. Goat milk kefir and vitamin D3 have potential as antioxidants and anti-inflammatory agents that can repair damage to pancreatic β cells. This study analyzed the effects of goat milk kefir fortified with vitamin D3 on the IL-18 level in diabetic rats. An experimental randomized pre-post test with control group design was conducted on 20 male Wistar rats divided into four groups, namely negative control (K-), positive control (K+), treatment with unfortified kefir (P1), and treatment with kefir fortified with vitamin D3. The intervention lasted 34 days. Fasting blood glucose and IL-18 levels were measured before and after intervention. Blood glucose and IL-18 levels were analyzed using the glucose oxidase p-aminophenol method and enzyme-linked immunosorbent assay, respectively. No significant increase in the IL-18 level was found in the P1 group with a median of 56.5 (10–252.7) pg/mL to 148.2 (106.8–428.3) pg/mL (p = 0.465) or P2 group with a median of 117.3 (91.8–146.8) pg/mL to 246.7 (168.8–311) pg/mL (p = 0.068), and no significant increase was observed in blood glucose levels in the P1 group (366.9±134.8 mg/dL to 462.1±156.9 mg/dL, p = 0.357) or P2 group (415.0±203.8 mg/dL to 258±129 mg/dL, p = 0.463). Goat milk kefir fortified with vitamin D3 could maintain blood glucose and IL-18 levels.

UJI POTENSI EKSTRAK DAUN BENALU BATU (BEGONIA SP) TERHADAP KADAR GLUKOSA DALAM DARAH DAN GAMBARAN HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (RATTUS NORVEGICUS)

2020 ◽  
Vol 6 (2) ◽  
pp. 286
Author(s):  
Joni Tandi ◽  
Dela Erni Paerunan ◽  
Nurifa Nurifa ◽  
Yunlis Silintowe Kenta ◽  
Sri Mulyani
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Glucose Levels ◽  
White Rats ◽  
Group I ◽  
Ethanol Extracts

This study aims to determine the potential for glucose levels in the blood of ethanol extracts of the begonias (Begonia Sp.), the effect of the extract on the pancreatic histopathology of white rats (Rattus norvegicus) which was induced by streptozotocin, and the effective dose begonias’ extract leaves on the histopathological descriptions of pancreatic male white rats. This study was an experimental laboratory where the research group consisted of group I (the control group) as a normal control was given a standard feed, group II (negative control) was given a 0.5% Na CMC suspension, group III (positive control) was orally given glibenclamide and experimental groups IV, V, and VI (doses 50, 100, and 200 mg/kg BW). Were given ethanol extract of begonias leaves. The results showed that the ethanol extract of the begonias (Begonia Sp.) contained secondary metabolite compounds, namely flavonoids, alkaloids, saponins, polyphenols, and tannins. Ethanol extract of begonias leaves at adose of 50 mg/kg BW was an affective dose in reducing blood glucoselevels by an avarage of 101,8 mg/dL) and a dose of 50 mg/kg BW can affect the histopathological picture of the pancreas in STZ induuced rats with an average damage value of 2 from a maximum of 4.

scholarly journals Ekstrak Etanol Kulit Buah Jengkol Menurunkan Kadar Glukosa dan Meningkatkan Hormon Insulin Tikus Diabetes Yang Diinduksi Streptozotocin

2019 ◽  
Vol 20 (1) ◽  
pp. 65
Author(s):  
Desak Made Malini ◽  
Madihah Madihah ◽  
Dita Aprila Khoirunnisa ◽  
Indriani Sasmita ◽  
Nining Ratningsih ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Blood Glucose ◽  
Reference Group ◽  
Ethanol Extract ◽  
Insulin Level ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Female Rats ◽  
Fruit Peel

Diabetes Mellitus (DM) is a disease with metabolism disorder signed by increasing of glucose blood (hyperglicemia), caused by disorder of insulin secretion and or increasing of insulin resistance. Therapy of jengkol’s fruit peel is an alternative treatment for DM. This study aims to determine the effect of ethanol extract of jengkol’s fruit peel to decrease glucose and increase insulin level of Wistar female rats which induced by streptozotocin (STZ). This research used the experimental method of Completely Randomized Design (RAL). Rat were first induced by single intravenous injection of STZ except Negative Control. Furthermore, rats were divided into six groups, namely Negative Control (NC), Positive Control (PC), Reference Group (RG) glibenclamide 10 mg/kg BW, and ethanol extract of jengkol’s fruit peel (EEOJFP) treatment at dose of 385 (P1), 770 (P2), and 1540 (P3) mg/kg BW with four replications each. Treatment was administered orally for 14 days. Data were analyzed by analysis of variance (ANAVA) with 95% confidence level and Duncan Multiple Range Test. The result showed that treatment of EEOJFP with dose of 385, 770 and 1540 mg/ kgBW decreased blood glucose 28.6%;65.4% and 59.1% successively, increased insulin as 54%; 230% and 206%. There was a protective effect of EEOJEF to level of blood glucose and insulin secretion of rat-STZ induced. The conclusion from this research is Ethanol extract of Jengkol’s fruit peel at dose 770 mg/kg BW is an effective dose in decreasing the level of glucose and increasing the level of insulin hormone in STZinduced diabetic rats. 

scholarly journals Phytochemical Screening and Antidiabetic Activity Test of Extracts and Fractions of Lactuca Indica (L.) In Streptozotocin-Induced Diabetic Mice

1970 ◽  
Vol 8 (3) ◽  
pp. 62-65
Author(s):  
Tamba Rosanto ◽  
Nainggolan Marline ◽  
Rosidah Noersal
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Antidiabetic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Group I ◽  
Reduce Blood Glucose ◽  
Lactuca Indica

Objectives: The purpose of this study was to determine the antidiabetic effect of ethanol extract and Lactuca indica leaf fraction to determine the effective dose of mice induced with streptozotocin compared with metformin. Design: The design of this study was experimental in which the extraction and fraction of Lactuca indica leaves were tested for the value of decreasing blood glucose levels of mice after induction of streptozotocin. Antidiabetic activity tests were divided into 12 groups. Group I (baseline) group II (negative control) were given CMC 0.5%, Group III (positive control) were given metformin 65 mg/kg BW, while Groups IV to XII were given Lactuca indica leaf extract and fractions at their respective doses -each 100, 150 and 200 mg/kg BW Interventions: The variable that was intervened in this study was the concentration of extract used Main outcome measure: The main measurement results in this study were to determine extracts and fractions that we're able to reduce blood glucose levels in mice. Results: The antidiabetic effect of Lactuca indica leaves shows that EELL, EAFLL and NHFLL have antidiabetic effects, this is supported by the chemical content contained in the extracts, namely flavonoids, tannins, saponins, glycosides and triterpenoids/steroids. The most effective activity to reduce blood glucose levels in streptozotocin-induced mice is EAFLL 100 mg/kg BW Conclusion: ethyl acetate fraction of Lactuca indica leaves has an effective antidiabetic activity at a dose of 200 mg/kg BW has given antidiabetic activity in mice induced by STZ, on the 9th day BGL mice have dropped below 120 mg/dl.  

Sign in / Sign up

Export Citation Format

Share Document