scholarly journals Isolation of Arsenic Oxidizing-reducing Bacteria and Reclamation of As (III) in in vitro Condition

2016 ◽  
Vol 19 (2) ◽  
pp. 99-101
Author(s):  
UA Naher ◽  
F Rahman ◽  
SMM Islam ◽  
MIU Sarkar ◽  
JC Biswas
Keyword(s):  
Contaminated Soil ◽  
Water Samples ◽  
Broth Culture ◽  
Plate Count ◽  
Bacterial Strains ◽  
Oxidation Activity ◽  
Plate Count Method ◽  
Reducing Bacteria ◽  
Soil And Water

CORRECTION: Due to a number of formatting and layout issues, the PDF of this paper was replaced on 10th October 2016. The page numbers of this article have changed from 94-96 to 99-101.The study aimed to isolate arsenic (As) oxidizing-reducing bacteria from As contaminated soil and water and to determine their ability to remove As from broth culture. Soil and water samples were collected from As contaminated area of BRRI farm, Bhanga, Faridpur. Arsenic oxidizing and reducing bacteria were isolated from the As contaminated soil (13 mg kg-1) and water (410 ?g/L) using spread plate count method in minimal salt (MS) medium after seven days of incubation. The oxidation activity of the bacteria as well as reclamation of As [III] was determined from NaAsO2 supplemented broth using atomic absorption spectrophotometer (AAS). The As-oxidizing bacteria population was present only in one soil sample, while in all water samples both oxidizing and reducing bacteria were present. A total of 36 As oxidizing-reducing bacteria were isolated. As-oxidizing bacteria removed 71-99% of applied As [III] in broth culture. Two out of 10 As-oxidizing bacterial strains appeared more efficient than others to remove As [III] under broth culture conditions.Bangladesh Rice j. 2015, 19(2): 99-100

scholarly journals Multiregional Evaluation of the SimPlate Heterotrophic Plate Count Method Compared to the Standard Plate Count Agar Pour Plate Method in Water

2000 ◽  
Vol 66 (1) ◽  
pp. 453-454 ◽  
Author(s):  
R. Wayne Jackson ◽  
Karen Osborne ◽  
Gary Barnes ◽  
Carol Jolliff ◽  
Dianna Zamani ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Water Samples ◽  
Plate Count ◽  
Heterotrophic Plate Count ◽  
Plate Method ◽  
Standard Plate Count ◽  
Plate Count Agar ◽  
Count Method ◽  
Standard Plate ◽  
Plate Count Method

ABSTRACT A new SimPlate heterotrophic plate count (HPC) method (IDEXX Laboratories, Westbrook, Maine) was compared with the pour plate method at 35°C for 48 h. Six laboratories tested a total of 632 water samples. The SimPlate HPC method was found to be equivalent to the pour plate method by regression analysis (r = 0.95;y = 0.99X + 0.06).

scholarly journals Black Currant (Ribes nigrumL.) and Bilberry (Vaccinium myrtillusL.) Fruit Juices Inhibit Adhesion ofAsaiaspp.

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Hubert Antolak ◽  
Agata Czyzowska ◽  
Dorota Kregiel
Keyword(s):  
Polyethylene Terephthalate ◽  
Culture Media ◽  
Carbon Sources ◽  
Fruit Juices ◽  
Plate Count ◽  
Bacterial Strains ◽  
Black Currant ◽  
Medium Carbon ◽  
Abiotic Surfaces ◽  
Plate Count Method

The aim of the study was to evaluate the activity of high-polyphenolic black currant (Ribes nigrumL.) and bilberry (Vaccinium myrtillusL.) juices against bacterial strainsAsaia lannensisandAsaia bogorensisisolated as spoilage of commercial soft drinks. The composition of fruit juices was evaluated using chromatographic techniques HPLC and LC-MS. The adhesion to glass, polystyrene, and polyethylene terephthalate in two different culture media was evaluated by luminometry and the plate count method. The major anthocyanins in theV. myrtilluswere petunidin-3-glucoside, malvidin-3-glucoside, cyanidin-3-glucoside, and delphinidin-3-glucoside, while inR. nigrumdelphinidin-3-rutinoside and cyanidin-3-rutinoside were detected. The LC-MS analysis showed presence of anthocyanins (delphinidin, cyanidin, petunidin, and malvidin derivatives), phenolic acids (chlorogenic and neochlorogenic acids), flavonols (quercetin-3-glucoside, quercetin-3-rutinoside), and flavanols (procyanidin B2 and procyanidin type A2). Additionally, in the bilberry juice A type procyanidin trimer was detected. The adhesion ofAsaiaspp. cells depended on the type of medium, carbon sources, and the type of abiotic surfaces. We noted that the adhesion was significantly stronger in minimal medium containing sucrose. The addition of bilberry and black currant juices notably reduced bacterial growth as well as cell adhesion to polyethylene terephthalate surfaces.

scholarly journals Screening and optimization of arsenic degrading bacteria and their potential role in heavy metal bioremediation

2019 ◽  
Vol 35 (4) ◽  
Author(s):  
Mehwish Iqtedar ◽  
Farah Aftab ◽  
Rabab Asim ◽  
Roheena Abdullah ◽  
Afshan Kaleem ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Arsenic Concentration ◽  
Cost Effective ◽  
Maximum Growth ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
High Concentration ◽  
Arsenic Tolerance ◽  
Degrading Bacteria ◽  
Soil And Water

Industrialization has added extremely toxic metalloid arsenic into the environment which at high concentration severely threatens the biota. Naturally, some microbes possess the ability to bio-accumulate metals and also to transform arsenite (As III) a toxic form to a non-toxic arsenate As V. The present study aimed to isolate arsenic resistant bacterias from the arsenic contaminated soil and water. Among eleven bacterial isolates, three FAs 1, 4 and 9 exhibited tolerance against sodium arsenite at 100mM concentration by achieving growth of 7.48×109,1.57×109 and 2.23×109 C.F.U./ml, respectively. Optimization at different conditions such as temperature, pH and arsenic concentration revealed high arsenic tolerance from isolate FAs 4 (5.33×108) at 37°C and FAs 1 (4.43×108 C.F.U./ml) at pH 7. Arsenic resistance at optimum conditions for the bacterial strains FAs 1, FAs 4 and FAs 9 showed maximum growth at 80mM concentration of arsenite. These bacterial isolates did not show redox ability to oxidize arsenite As III to arsenate As V. However bacterial isolates FAs 1, FAs 4 and FAs 9 were able to accumulate arsenic 39.16, 148 and 125 µg/L on the 4th, 3rd and 5th day of incubation, respectively. The isolates FAs 1, FAs 4 and FAs 9 were identified as Gram negative non endospore forming rods. In future, these novel isolates possess a great potential in biotechnology field, as bioremediation of arsenic contaminated soil and water can be done by employing arsenic accumulating bacteria which is an eco-friendly and cost effective method.

Identification of Lyoprotectants Released from Gradient-freezing Pretreated Entocyte in Lactococcus Lactis Subsp. Lactis IL1403

2017 ◽  
Vol 17 (2) ◽  
pp. 122-134
Author(s):  
Lin Songyang ◽  
Kang Qiaozhen ◽  
Pan Dan ◽  
Liu Xin ◽  
Lu Laizheng ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Starter Culture ◽  
Pentose Phosphate ◽  
Plate Count ◽  
Phosphotransferase System ◽  
Lactococcus Lactis Subsp ◽  
Plate Count Method ◽  
Lactis Il1403 ◽  
Related Proteins

Performance of Lactococcus lactis as a starter culture in food production largely depends on the use of lyoprotectants during lyophilization. Gradient-freezing of bacterial cultures was conducted at 4°C, −20°C, and −80°C by storing the samples at each temperature for 2 h, successively. The entocytes extracted from the frozen cells were used as a lyoprotectant in the follow-up freeze-drying process of Lactococcus lactis subsp. lactis IL1403. The cell survival rate of gradient-freezing group increased 6.4-fold by bacterial plate count method. Furthermore, a proteomics and bioinformatics method was applied to elucidate the protein changes of Lactococcus lactis subsp. lactis IL1403 in response to gradient-freezing by gel-free proteomics using tandem mass tags (TMTs). The results showed that 121 stress-related proteins were significantly influenced by gradient-freezing. These proteins were involved in several metabolism pathways including ribosome metabolism, amino acid metabolism, quorum sensing, phosphotransferase system (PTS), pentose phosphate pathway, microbial metabolism in diverse environments, and nitrogen metabolism, etc. Some of these proteins especially the up-regulated proteins are potential lyoprotectants in vitro and they still need to be further investigated.

scholarly journals MPN Drop Agar Method for Determination of Heterotrophic Microorganisms in Soil and Water Samples Using Tissue Plate as a Carrier

2020 ◽  
Vol 12 (19) ◽  
pp. 8252
Author(s):  
Jana Chumchalová ◽  
Martin Kubal
Keyword(s):  
Water Samples ◽  
Environmental Microbiology ◽  
Most Probable Number ◽  
Bacterial Strains ◽  
Laboratory Equipment ◽  
Probable Number ◽  
Heterotrophic Microorganisms ◽  
Wide Range ◽  
Glass Tubes ◽  
Soil And Water

The most probable number (MPN) method is a culture-based method commonly used in the field of environmental microbiology to examine microbial populations in liquid substrates. The MPN tests require a wide range of special laboratory equipment, a lot of laboratory space, and skilled staff, which together limit their applicability. This paper presents a modified MPN method, which reduces the experimental requirements by applying tissue plate as a carrier. The modified MPN method introduces a fast-filled tissue plate with 5 × 5 squares as agar carrier, instead of the commonly used set of glass tubes. Further, self-refilling automatic syringe Socorex® was implemented to apply samples to the plate. The response of the modified MPN method was tested on eight selected bacterial strains as well as on soil and water samples. Simultaneously, all the strains and samples were tested by standard spread plate method. High linear correlation between the two methods was found, which makes a new modified MPN method a useful alternative within the field of environmental microbiology.

Reduction of hexavalent chromium using fungi and bacteria isolated from contaminated soil and water samples

2013 ◽  
Vol 29 (4) ◽  
pp. 320-328 ◽  
Author(s):  
Reuel M. Bennett ◽  
Paul Rodrigo F. Cordero ◽  
Gershon S. Bautista ◽  
Gina R. Dedeles
Keyword(s):  
Hexavalent Chromium ◽  
Contaminated Soil ◽  
Water Samples ◽  
Soil And Water

COUNTING TOTAL BACTERIA IN LAND ORGANIC WASTE HOUSEHOLD AND LAND INORGANIC WITH TOTAL PLATE COUNT METHOD (TPC)

2017 ◽  
Vol 4 (2) ◽  
pp. 87-91
Author(s):  
Ekamaida Ekamaida
Keyword(s):  
Soil Fertility ◽  
Bacterial Population ◽  
Organic Soil ◽  
Biological Properties ◽  
Plate Count ◽  
Bacterial Populations ◽  
Fertility Data ◽  
Plate Count Method ◽  
The Mean ◽  
Total Bacteria

The soil fertility aspect is characterized by the good biological properties of the soil. One important element of the soil biological properties is the bacterial population present in it. This research was conducted in the laboratory of Microbiology University of Malikussaleh in the May until June 2016. This study aims to determine the number of bacterial populations in soil organic and inorganic so that can be used as an indicator to know the level of soil fertility. Data analysis was done by T-Test that is by comparing the mean of observation parameter to each soil sample. The sampling method used is a composite method, which combines 9 of soil samples taken from 9 sample points on the same plot diagonally both on organic soil and inorganic soil. The results showed the highest bacterial population was found in total organic soil cfu 180500000 and total inorganic soil cfu 62.500.000

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