scholarly journals Antagonistic potential of rhizosphere fungi against leaf spot and fruit rot pathogens of brinjal

2015 ◽  
Vol 43 (2) ◽  
pp. 213-217 ◽  
Author(s):  
MT Aktar ◽  
KS Hossain ◽  
MA Bashar
Keyword(s):  
Trichoderma Harzianum ◽  
Fusarium Moniliforme ◽  
Soil Fungi ◽  
Pathogenic Fungi ◽  
Fruit Rot ◽  
Curvularia Lunata ◽  
Volatile Metabolites ◽  
Rhizosphere Fungi ◽  
Leaf Spots ◽  
Antagonistic Potential

Antagonistic potentials of seven rhizoshere soil fungi viz., Aspergillus flavus Link., A. fumigatus Fresen., A. niger Tiegh., A. terreus Thom., Penicillium sp., Trichoderma harzianum Refat. and T. viride Pers. were tested in opposition to six pathogenic fungi viz., Colletotrichum sp., Curvularia lunata, Fusarium moniliforme, F. oxysporum, F. semitectum and Phomopsis sp. isolated from different leaf spots and fruit rots of brinjal. Out of seven soil fungi, Trichoderma harzianum was found most effective to control the growth of all the test fungi in the study of colony interactions and effects of volatile and non-volatile metabolites. This fungus may be exploited commercially to biocontrol the diseases. DOI: http://dx.doi.org/10.3329/bjb.v43i2.21675 Bangladesh J. Bot. 43(2): 213-217, 2014 (September)

scholarly journals Antagonistic potential of rhizosphere mycoflora against Fusarial wilt of brinjal

2015 ◽  
Vol 24 (2) ◽  
pp. 137-145
Author(s):  
Romana Akter ◽  
KS Hossain ◽  
MA Bashar
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Trichoderma Harzianum ◽  
Radial Growth ◽  
Soil Fungi ◽  
Volatile Metabolites ◽  
Penicillium Sp ◽  
Maximum Inhibition ◽  
Antagonistic Potential

Antagonistic potentials of seven rhizoshere soil fungi viz., Aspergillus flavus Link., A. fumigatus Fresen., A. niger Tiegh., A. terreus Thom., Penicillium sp., Trichoderma harzianum Refat. and T. viride Pers. were tested against the Fusarium oxysporum Schlecht. and F. solani (Mart.) Sacc. – the two pathogens, isolated from wilted roots of brinjal plants. Out of seven soil fungi Trichoderma harzianum was found most effective to control the growth of both the test pathogens. More than 50% inhibition of the test pathogens were observed with T. viride, Aspergillus niger and A. fumigatus in colony interaction. The maximum inhibition of radial growth of the test pathogens was observed with volatile metabolites and cell free culture filtrates of Trichoderma harzianum. This fungus may be exploited commercially to control the disease. Dhaka Univ. J. Biol. Sci. 24(2): 137-145, 2015 (July)

scholarly journals Antagonistic Potentials of Selected Soil Fungi Against Three Pathogenic Fungi of Tagetes Erecta L. and T. Patula L

2020 ◽  
Vol 29 (2) ◽  
pp. 147-153
Author(s):  
Mahfuza Aktar ◽  
Shamim Shamsi
Keyword(s):  
Radial Growth ◽  
Soil Fungi ◽  
Trichoderma Viride ◽  
Pathogenic Fungi ◽  
Complete Inhibition ◽  
Curvularia Lunata ◽  
Dual Culture ◽  
Volatile Metabolites ◽  
Culture Filtrates ◽  
Maximum Inhibition

Three antagonistic fungi were isolated from the field soil of blight infected Tagetes spp. by serial dilution method. The fungi were identified as Aspergillus flavus Link, A. niger van Tiegh. and Trichoderma viride Pers. Antagonistic potentiality of aforesaid fungi were evaluated against the pathogenic fungi of Tageteserecta L. and T. patula L. following "dual culture colony interaction" and volatile and nonvolatile metabolites. The pathogenic fungi were A. alternata, Aspergillus fumigatus and Curvularia lunata. In dual culture colony interaction, out of three soil fungi, T. viride showed the highest growth inhibition on A. alternata (71.03%), A. fumigatus (38.49%) and C. lunata (60.71%). The maximum inhibition of radial growth of A. alternata (74.55%) was observed with the culture filtrates of T. viride owing to volatile metabolites. The maximum inhibition of radial growth of A. fumigatus (37.43%) was observed with the culture filtrates of A. flavus owing to volatile metabolites. The complete inhibition of radial growth of C. lunata was observed with the culture filtrates of A. niger owing to volatile metabolites. The complete inhibition of radial growth of A. alternata was observed with nonvolatile metabolites of A. nigera and T. viride at all concentrations. The complete inhibition of radial growth of A. fumigatus was also observed with nonvolatile metabolites of A. niger at all concentrations. Aspergillus niger and T. viride may be exploited commercially as a biocontrol agent against blight pathogens of T. erecta and T. patula. Dhaka Univ. J. Biol. Sci. 29(2): 147-153, 2020 (July)

scholarly journals In Vitro Antagonism of Trichoderma Verede and Aspergillus Spp. against a Pathogenic Seed Borne Fungus of Sesame

2019 ◽  
Vol 43 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Md Delwar Hosen ◽  
Shamim Shamsi
Keyword(s):  
Aspergillus Flavus ◽  
Soil Fungi ◽  
Trichoderma Viride ◽  
Dual Culture ◽  
Volatile Metabolites ◽  
Inhibiting Effect ◽  
Antagonistic Potential ◽  
Academy Of Sciences ◽  
Growth Inhibiting

Four soil fungi were isolated from the soil by serial dilution and were identified as Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tieghem and Trichoderma viride Pers. The soil fungi were selected to evaluate their antagonistic potential against seed borne fungus Fusarium merismoides isolated from sesame. In dual culture colony interaction Trichoderma viride showed the highest (45.88%) growth inhibiting effect on F. merismoides followed by A. niger (40.00%), A. flavus (36.37) and A. fumigatus (30.77%). Volatile metabolites from T. viride showed the highest growth inhibiting effect on F. merismoides (67.69%) and non-volatile metabolites from T. viride showed the highest growth inhibiting effect on F. merismoides (75.00%). Journal of Bangladesh Academy of Sciences, Vol. 43, No. 1, 17-23, 2019

scholarly journals In vitro evaluation of fungicides and plant extracts against pathogenic fungi of two rice varieties

2018 ◽  
Vol 44 (2) ◽  
pp. 251-259
Author(s):  
Pranami Chowdhury ◽  
MA Bashar ◽  
Shamim Shamsi
Keyword(s):  
Plant Extracts ◽  
Radial Growth ◽  
Fusarium Moniliforme ◽  
Mangifera Indica ◽  
Pathogenic Fungi ◽  
Curvularia Lunata ◽  
Asparagus Racemosus ◽  
Rice Varieties ◽  
Datura Metel

Five pathogenic fungi viz., Alternaria alternata (Fr.) Keissler, Curvularia lunata (Wakker) Boedijn, Drechslera oryzae Breda de Haan (Subramanian and Jain), Fusarium moniliforme Sheldon and Pestalotiopsis guepinii (Desm.) Stay. were isolated from two rice varieties viz., BRRI 29 (Boro) and Pajam (Aman) by Blotter and Tissue planting methods. Ten fungicides i.e. Bavistin 50 WP, Salcox 50 WP, Dithane M-45, Indofil M-45, Tall 25 EC, Ridomil MZ Gold, MC Sulphur 80, Greengel, Hayvit 80 WP and Capvit 50 WP at 100, 200, 300, 400 and 500 ppm were evaluated against the above mentioned five pathogenic fungi. Tall 25 EC completely inhibited the radial growth of the test fungi at all the concentrations except Fusarium moniliforme. Antifungal properties of ethanol extract of Artocarpus heterophyllus Lamk., Tagetes erecta L., Datura metel L., Senna alata (L.) Roxb., Azadirachta indica A. Juss., Citrus medica L., Mangifera indica L., Asparagus racemosus Willd., Nerium indicum Mill. and Allium sativum L. at 5, 10 and 20% concentrations were evaluated against the five test pathogens. All the plant extracts completely inhibited the radial growth of the test fungi at 20% concentration except Asparagus racemosus.

scholarly journals In vitro screening of fungicides and plant extracts against six pathogenic fungi isolated from cotton (Gossypium arboreum L.) seed

2020 ◽  
Vol 49 (2) ◽  
pp. 197-204
Author(s):  
Mst Nazin Nahar ◽  
Shamim Shamsi
Keyword(s):  
Growth Inhibition ◽  
Rhizoctonia Solani ◽  
Fusarium Moniliforme ◽  
Pathogenic Fungi ◽  
Psidium Guajava ◽  
Curvularia Lunata ◽  
Type I ◽  
Fusarium Sporotrichioides ◽  
Datura Metel

Six pathogenic fungi, namely Aspergillus flavus Link., A. niger van Tieghem (Type-I)., Curvularia lunata (Wakker) Boedijn, Fusarium moniliforme var. subglutinans Wr. & Reink., Fusarium sporotrichioides Sherb., Mem. and Rhizoctonia solani J.G. Kuhn were isolated from cotton seeds. Five fungicides viz., Acrobat MZ, Autostin 50 WDG, Capvit 50 WP, Nativo 75 WP and Thiovit 80 WG were selected to evaluate in vitro efficacy at 100, 200, 300, 400 and 500 ppm concentrations against pathogenic fungi following poisoned food technique. Out of 5 fungicides Nativo 75 WP showed the complete growth inhibition of above mentioned six pathogenic fungi at all the used concentrations. Autostin 50 WDG showed complete growth inhibition of all tested pathogenic fungi except Curvularia lunata. Leaf extract of five angiospermic plants viz., Adhatoda vasica, Aegle mermelos, Azadirachta indica, Datura metel and Psidium guajava were selected to evaluate in vitro fungitoxicity at 5, 10, 15 and 20% concentrations against the test pathogens. At 20% concentration, out of the 5 plants extracts A. indica was found to be most active to inhibit the growth of Aspergillus niger (Type-I) (65.56%) and Fusarium moniliforme var. subglutinans (75.00%), Psidium guajava were most active against A. flavus (81.29%) and Curvularia lunata (72.23%), and Datura metel was most active against Fusarium sporotrichioides (64.77%) and Rhizoctonia solani (42.44%).

scholarly journals Antagonistic potentials of some soil fungi against three post‐harvest pathogenic fungi of Carica papaya L.

2019 ◽  
Vol 28 (1) ◽  
pp. 1-7
Author(s):  
Rezuana Binte Helal ◽  
Shamim Shamsi
Keyword(s):  
Growth Inhibition ◽  
Radial Growth ◽  
Soil Fungi ◽  
Carica Papaya ◽  
Trichoderma Viride ◽  
Pathogenic Fungi ◽  
Dual Culture ◽  
Volatile Metabolites ◽  
Maximum Inhibition ◽  
Fusarium Sp

Four antagonistic fungi were isolated from the papaya field soil by serial dilution and were identified as Aspergillus flavus Link., A. fumigatus Fresenius, A. niger van Tiegh. and Trichoderma viride Pers. The soil fungi were selected to evaluate their antagonistic potentiality against the three postharvest pathogenic fungi of Carica papaya L. following "dual culture colony interaction” and volatile and non-volatile metabolites. In dual culture colony interaction, out of four soil fungi, T. viride showed the highest growth inhibition on C. gloeosporioides (84.28%), F. nivale (95.23%) and Fusarium sp. (87.15%). On the other hand A. niger showed the highest growth inhibition on C. gloeosporioides (77.39%), F. nivale (98.63%) and Fusarium sp. (35.05%). The maximum inhibition of radial growth of C. gloeosporioides (77.64%), F. nivale (58.76%) and Fusarium sp. (79.37%). were observed in case of T. viride owing to the volatile metabolites. Whereas the maximum inhibition of radial growth of C. gloeosporioides (58.23%), F. nivale (37.43%) and Fusarium sp. (82.31%) were observed in case of A. niger owing to the volatile metabolites. The maximum inhibition of radial growth of C. gloeosporioides (90.90%), F. nivale (89.13%) and Fusarium sp. (76.84%) were observed in case of T. viride owing to the effect of non-volatile metabolites. The maximum inhibition of radial growth of C. gloeosporioides (92.42%), F. nivale (73.01%) and Fusarium sp. (68.67%) were observed in case of A. niger owing to the effect of non-volatile metabolites. Trichoderma viride and A. niger may be exploited commercially as a biocontrol agent against anthracnose and fruit rot pathogens of papaya. Dhaka Univ. J. Biol. Sci. 28(1): 1-7, 2019 (January)

scholarly journals In vitro interactions between Trichoderma harzianum and pathogenic fungi damaging horse-chestnut (Aesculus hippocastanum) leaves and fruits

2019 ◽  
Vol 54 (1) ◽  
pp. 21-35
Author(s):  
Katarína Pastirčáková
Keyword(s):  
Trichoderma Harzianum ◽  
Fungal Pathogens ◽  
Mutual Influence ◽  
Pathogenic Fungi ◽  
Antagonistic Effect ◽  
Aesculus Hippocastanum ◽  
Horse Chestnut ◽  
Mycelium Growth ◽  
Antagonistic Potential

Abstract Interactions between 3 pathogenic fungi damaging horse-chestnut (Aesculus hippocastanum) leaves and fruits – Phyllosticta sphaeropsoidea, Phomopsis carposchiza, and Diaporthe padi – and the antagonistic fungus Trichoderma harzianum were studied to determine their mutual influence in vitro. Antibiosis of colonies developing on 5 nutrient media was tested. The 3 studied T. harzianum isolates differed in their antagonistic potential. although T. harzianum isolates significantly inhibited the growth of Phomopsis carposchiza, the mycelium growth of some of the re-isolates on fresh medium indicates an inadequate antagonistic effect of T. harzianum on this species. The tested Trichoderma isolates showed stronger antagonism towards the other pathogens, reflected in overgrowing of Phyllosticta sphaeropso-idea and Diaporthe padi and reducing their growth. Granulation of the cytoplasm and lysis of hyphae of the fungal pathogens were the most frequently observed effects of the interaction.

scholarly journals Diversity of fungal pathogens associated with loquat and development of novel virulence scales

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0257951
Author(s):  
Muhammad Fahim Abbas ◽  
Sana Batool ◽  
Sobia Khaliq ◽  
Sidra Mubeen ◽  
Azziz-ud-Din ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Fruit Rot ◽  
Eriobotrya Japonica ◽  
Curvularia Lunata ◽  
Biotic And Abiotic Stresses ◽  
Management Options ◽  
Leaf Spots ◽  
Bt Gene ◽  
First Time ◽  
Comprehensive Study

Loquat [Eriobotrya japonica (Thunb.) Lindl.] is an important fruit crop in Pakistan; however, a constant decline in its production is noted due biotic and abiotic stresses, particularly disease infestation. Fungal pathogens are the major disease-causing agents; therefore, their identification is necessary for devising management options. This study explored Taxila, Wah-Cantt, Tret, Chatar, Murree, Kalar-Kahar, Choa-Saidan-Shah and Khan-Pur districts in the Punjab and Khyber Paktoon Khawa (KPK) provinces of Pakistan to explore the diversity of fungal pathogens associated with loquat. The samples were collected from these districts and their microscopic characterizations were accomplished for reliable identification. Alternaria alternata, Curvularia lunata, Lasiodiplodia theobromae, Aspergilus flavis, Botrytis cinerea, Chaetomium globosum, Pestalotiopsis mangiferae and Phomopsis sp. were the fungal pathogens infesting loquat in the study area. The isolates of A. alternata and C. lunata were isolated from leaf spots and fruit rot, while the isolates of L. theobromae were associated with twig dieback. The remaining pathogens were allied with fruit rot. The nucleotide evidence of internal transcribed spacer (ITS) regions (ITS1, 5.8S, and ITS2) were computed from all the pathogens and submitted in the database of National Center for Biotechnology Information (NCBI). For multigene analysis, beta-tubulin (BT) gene and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) regions were explored for A. alternata and C. lunata isolates, respectively. The virulence scales of leaf spots, fruit rot, and twig dieback diseases of loquat were developed for the first time through this study. It is the first comprehensive study with morpho-molecular identification, and newly developed virulence scales of the fungal pathogens associated with loquat, which improves the understanding of these destructive diseases.

scholarly journals Biocontrol potential of Streptomyces sp. CACIS-1.5CA against phytopathogenic fungi causing postharvest fruit diseases

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Zahaed Evangelista-Martínez ◽  
Erika Anahí Contreras-Leal ◽  
Luis Fernando Corona-Pedraza ◽  
Élida Gastélum-Martínez
Keyword(s):  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Fruit Rot ◽  
Main Body ◽  
Type I ◽  
Tropical Fruit ◽  
Habanero Pepper ◽  
Antagonistic Microorganisms

Abstract Background Fungi are one of the microorganisms that cause most damage to fruits worldwide, affecting their quality and consumption. Chemical controls with pesticides are used to diminish postharvest losses of fruits. However, biological control with microorganisms or natural compounds is an increasing alternative to protect fruits and vegetables. In this study, the antifungal effect of Streptomyces sp. CACIS-1.5CA on phytopathogenic fungi that cause postharvest tropical fruit rot was investigated. Main body Antagonistic activity was evaluated in vitro by the dual confrontation over fungal isolates obtained from grape, mango, tomato, habanero pepper, papaya, sweet orange, and banana. The results showed that antagonistic activity of the isolate CACIS-1.5CA was similar to the commercial strain Streptomyces lydicus WYEC 108 against the pathogenic fungi Colletotrichum sp., Alternaria sp., Aspergillus sp., Botrytis sp., Rhizoctonia sp., and Rhizopus sp. with percentages ranging from 30 to 63%. The bioactive extract obtained from CACIS-1.5 showed a strong inhibition of fungal spore germination, with percentages ranging from 92 to 100%. Morphological effects as irregular membrane border, deformation, shrinkage, and collapsed conidia were observed on the conidia. Molecularly, the biosynthetic clusters of genes for the polyketide synthase (PKS) type I, PKS type II, and NRPS were detected in the genome of Streptomyces sp. CACIS-1.5CA. Conclusions This study presented a novel Streptomyces strain as a natural alternative to the use of synthetic fungicides or other commercial products having antagonistic microorganisms that were used in the postharvest control of phytopathogenic fungi affecting fruits.

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