scholarly journals Allelopathic Studies on Milk Thistle (Silybum marianum)

2013 ◽  
Vol 2 (1) ◽  
pp. 62-67 ◽  
Author(s):  
Shamima Sultana ◽  
Md Asaduzzaman
Keyword(s):  
Fresh Water ◽  
Seedling Growth ◽  
Water Extract ◽  
Hot Water ◽  
Plant Sample ◽  
Milk Thistle ◽  
Distilled Water ◽  
Silybum Marianum ◽  
Weed Species ◽  
Water Extracts

Declining crop yield due to weeds and their resistance to herbicides are major constraint for successful crop productions. Milk thistle (Silybum marianum) is common weed species in Australian cropping rotation. Allelopathic potentiality of milk thistle on different crops has been documented sporadically, but there is no literature on about ryegrass and canola. Therefore, a laboratory based allelopathic extracts bioassay was conducted. The hot water extracts was prepared from milk thistle plant parts added into water with ration of 1: 10 (plant sample: distilled water) where mixture was heated 10 minutes. After heat treatment samples was immediately sieved and centrifuged and the resulted solution was treated as 100% concentration. Separately, to get the fresh water extract plant sample was added into water (1:10) and kept 24 hours in room temperature. After 24 hours, the sample was sieved and centrifuged and collected samples result was treated 100% concentrations. To obtain 50% concentration, both hot and fresh samples were diluted with distilled water. Therefore the experiment was conducted with five different treatment concentrations (0, 50% hot water extracts, 50% fresh water extracts, 100% hot water extracts and 100% fresh water extracts). The experiment was comprised with RCBD design with three replications under control conditions. During experimental period the allelopathic effects of donor species on germination and seedling growth of ryegrass and canola was observed. Results shows, germination and seedling growth of both receiver species are inhibited by milk thistle extracts. Extracts from fresh water at 100% was more toxic to receiver species followed by 50% concentration of fresh and 100% from hot water extracts. This concentration reduced the root, shoot growth of ryegrass and canola 84.971%, 84.269% and 89.898%, 87.394%, respectively. The result also revealed that allelopathic pattern of hot water extracts was same however; it is less toxic to both receiver species. DOI: http://dx.doi.org/10.3329/ijarit.v2i1.14007 Int. J. Agril. Res. Innov. & Tech. 2 (1): 62-67, June, 2012

Pressurized water versus ethanol as a Silybum marianum extraction solvent for inhibition of low-density lipoprotein oxidation mediated by copper and J774 macrophage cellsThis article is one of a selection of papers published in this special issue (part 1 of 2) on the Safety and Efficacy of Natural Health Products.

2007 ◽  
Vol 85 (9) ◽  
pp. 894-902 ◽  
Author(s):  
Sunny N. Wallace ◽  
Jennifer Raible ◽  
Danielle Julie Carrier ◽  
Katherine L. Vaughn ◽  
Carl L. Griffis ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Low Density Lipoprotein ◽  
Water Extract ◽  
Density Lipoprotein ◽  
Hot Water ◽  
Milk Thistle ◽  
Low Density ◽  
Silybum Marianum ◽  
Pressurized Water ◽  
J774 Macrophage

Silybum marianum contains flavonolignans, termed silymarin (SM), that are therapeutic agents for many inflammation-based diseases including atherosclerosis. Oxidation of human low-density lipoprotein was induced by CuSO4 or J774 macrophage cells and measured by the formation of thiobarbituric acid reactive substances (TBARS). SM was extracted by pressurized hot water (PHWE) or ethanol, and the effects of these extracts on TBARS formation were evaluated in comparison with those of SM preparations made from blending masses of individual flavonolignan standards in ratios identical to those of the water and ethanol extracts. Ethanol-extracted SM and its blended counterpart inhibited the generation of TBARS by 82% and 43%, respectively, at 150 μmol/L doses. TBARS levels in the presence of 150 μmol/L of the PHWE and its blended SM counterpart were reduced by 84% and 38%, respectively. Extracts from milk thistle fruit displayed higher protective effects than blended SM solutions of the same concentration with an identical compositional makeup. The appearance of degradation peaks in the water extract did not create any cytotoxic effects. Results of this study confirm that PHWE can be used to extract flavonolignans from milk thistle and that these extracts may possess therapeutic potential different from or beyond that of traditional organic solvent preparations.

scholarly journals Antioxidant properties of hot water extracts from carpophore and spores of mushroom Ganoderma lucidum

2011 ◽  
pp. 279-288 ◽  
Author(s):  
Anita Klaus ◽  
Maja Kozarski ◽  
Miomir Niksic
Keyword(s):  
Antioxidant Activity ◽  
Ganoderma Lucidum ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Reducing Power ◽  
Hot Water ◽  
Ferrous Ions ◽  
Medicinal Mushrooms ◽  
Water Extracts ◽  
Hot Water Extract

Ganoderma lucidum (Leyss.:Fr.) Karst is one of the medicinal mushrooms, which possesses enviable antioxidant properties. Objective of this investigation was to evaluate antioxidant activity, reducing power, scavenging abilities on 1.1-diphenyl-2-picrylhydrazyl (DPPH) radicals and chelating effects on ferrous ions of hot water extracts obtained from carpophore and spores of this mushroom. Hot water extract from carpophore (Gl-I) showed high antioxidant activity of 85.7 ? 0.7%, at 10 mg/ml, while antioxidant activity of hot water extract from spores (Gl-Is) was 9.2 ? 0.3% at 10 mg/ml. Reducing power of Gl-I reached a plateau of 3.4 ? 0.1 at 20 mg/ml, and 0.3 ? 0.0 at 20 mg/ml for Gl-Is. At 10 mg/ml, scavenging ability on DPPH radicals of Gl-I increased to 96.8 ? 2.5%, whereas Gl-Is scavenged DPPH radicals by 69.6 ? 2.5% at 10 mg/ml. Gl-I chelated 81.6 ? 3.6 % of ferrous ions at 20 mg/ml, while the chelating effect of Gl-Is was 73.8 ? 1.7%. The antioxidative activities of hot water extracts from carpophore and spores of the mushroom G. lucidum were concentration dependent and increased with an increase in the concentration.

scholarly journals GERMINATION AND SEEDLING GROWTH OF SILYBUM MARIANUM AS A MEDICINAL PLANT UNDER SALINITY STRESS

2019 ◽  
pp. 25-38
Author(s):  
M. A. Dorri ◽  
B. Kamkar ◽  
M. Aghdasi ◽  
A. R. Safahani
Keyword(s):  
Medicinal Plant ◽  
Seedling Growth ◽  
Block Design ◽  
Germination Percentage ◽  
Milk Thistle ◽  
Tolerance Index ◽  
Root Weight ◽  
Silybum Marianum ◽  
Nacl Concentration ◽  
Salinity Levels

Milk thistle (Silybum marianum) is used as a medicinal plant in the treatment of liver diseases. In order to investigate germination and seedling growth in S. marianum subjected to NaCl, a three replicated experiment was carried out in a randomized complete block design in the laboratory of Gorgan University of Agricultural Sciences and Natural Resources. Treatments included seven salinity levels (50, 75, 100, 125, 150, 175 and 200 mM) and a control (distilled water). Germination percentage (GP), mean germination time (MGT), seed-ling vigour index (SVI), shoot, root and seedling weight were measured. The effect of salinity levels was significant on GP, SVI, MGT and seedling weight and length (p ≤ 0.01). However, the NaCl concentration effect was not significant on shoot : root length ratio and or shoot : root weight ratios. Results showed that germination decreased when salinity increased, while MGT increased. MGT was 1.75 times higher than in the control at the highest salt concentration. MGT difference was not significant between 75 mM NaCl and con-trol, while it dramatically increased by increasing the NaCl concentration from 150 to 200 mM NaCl. Reduc-tion slope and salt tolerance index (STI) were estimated for germination (0.54 and 231.9) and seedling stage (0.24 and 237.4). According to the results, milk thistle could be considered as a valuable medicinal plant in fairly salinized areas.

scholarly journals Comparative study of the antioxidant properties of silymarin and milk thistle extracts

2021 ◽  
Vol 19 (1) ◽  
pp. 1-6
Author(s):  
V. Ivanov ◽  
M. Pavlova
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Water Extract ◽  
Total Phenolic ◽  
Milk Thistle ◽  
Flavonoid Content ◽  
Silybum Marianum ◽  
Dpph Method ◽  
Phenolic And Flavonoid

Purpose: Milk thistle (Silybum marianum (L.) Gaertn) has been used for centuries as a natural remedy for liver and gall bladder / biliary tract diseases. Silymarin is the purified and standardized extract from milk thistle seeds. The purpose of the present study is to present a comparative analysis of the total phenolic substances content and the antioxidant effect of silymarin and three different types of Silybum marianum extracts (i.e. ethanol extract, water extract and lyophilized extract). Methods: Total phenolic and flavonoid content of extracts was analysed and the antioxidant activity was assessed by determining their free radical scavenging ability by DPPH method. Results: Total phenolic content is the highest in silymarin– 134.12±3.22 mg GAE/g, followed by lyophilized water extract – 51.74±2.10 mg GAE/g. Silymarin has also been found to have the highest antioxidant activity among all extract samples (338.03 VCEAC µmol /g), followed by S.marianum lyophilised extract (123.05 VCEAC µmol/g). Conclusions: A strong correlation between total phenolic and flavonoid content in the extracts and their antioxidant activity has been found.

Antioxidant Properties of Glossogyne tenuifolia

2006 ◽  
Vol 34 (04) ◽  
pp. 707-720 ◽  
Author(s):  
Joan-Hwa Yang ◽  
Shu-Yao Tsai ◽  
Ching-Mei Han ◽  
Chun-Chien Shih ◽  
Jeng-Leun Mau
Keyword(s):  
Cold Water ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Hot Water ◽  
Butylated Hydroxyanisole ◽  
Ferrous Ions ◽  
Water Extracts ◽  
Hot Water Extract ◽  
Ethanolic Extracts ◽  
Glossogyne Tenuifolia

Glossogyne tenuifolia (Labill) Cass. (Compositae) is a special medicinal plant in the Pescadores Islands. Ethanolic, cold and hot water extracts were prepared from the dried herb and their antioxidant properties and components were studied. Ascorbic acid, α-tocopherol, butylated hydroxyanisole, citric and ethylenediaminetetraacetic acids were used in assays for comparison. With regard to EC50 values in antioxidant activity, ethanolic and hot water extracts (0.08 and 0.09 mg/ml) were much more effective than the cold water extract (0.76 mg/ml). At 1.0 mg/ml, reducing capacities were 1.57, 0.31 and 1.04 for ethanolic, cold water and hot water extracts, respectively. Scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl radicals were in descending order: ethanolic > cold water > hot water extracts. At 20 mg/ml, the hot water extract chelated all hydroxyl ions (100%) whereas the scavenging ability of the cold water extract was 68.86%. Chelating abilities on ferrous ions were in descending order: cold water > hot water > ethanolic extracts. Phenols were found to be the major antioxidant components. All EC50 values were below 20 mg/ml, and some even below 0.1 mg/ml, indicating that all three extracts from G. tenuifolia were rich in antioxidant properties.

scholarly journals Subcritical Water Extracts from Agaricus blazei Murrill's Mycelium Inhibits the Expression of Immune Checkpoint Molecules and Axl receptor

2021 ◽  
Author(s):  
Taro Yasuma ◽  
Masaaki Toda ◽  
Hajime Kobori ◽  
Naoto Tada ◽  
Corina D'Alessandro-Gabazza ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Fruiting Body ◽  
Immune Checkpoint ◽  
Subcritical Water ◽  
Water Extract ◽  
Hot Water ◽  
Mouse Bone Marrow ◽  
Agaricus Blazei ◽  
Water Extracts ◽  
Immune Checkpoint Molecules

Agaricus blazei Murrill or Himematsutake is an edible and medicinal mushroom. Agaricus blazei Murrill's fruiting body extracts have anticancer properties, although the mechanism is unknown. Basic or organic solvents, which are hazardous for human health, are generally used to prepare Agaricus blazei Murrill's extracts. Inhibition of immune checkpoint molecules and Axl receptor is an effective therapy in cancer. This study assessed whether subcritical water extracts of the Agaricus blazei Murrill's fruiting body or mycelium affect the expression of Axl and immune checkpoint molecules in lung cancer cells. We used A549 cells and mouse bone marrow-derived dendritic cells in the experiments. We prepared subcritical water extracts from the Agaricus blazei Murrill's fruiting body or mycelium. The subcritical water extracts from the Agaricus blazei Murrill's fruiting body or mycelium significantly inhibited the expression of immune checkpoint molecules and Axl compared to saline-treated cells. Also, the hot water extract, subcritical water extract, and the hot water extraction residue subcritical water extract from the Agaricus blazei Murrill's mycelium significantly enhanced the expression of maturation markers in dendritic cells. These observations suggest that the subcritical water extract from Agaricus blazei Murrill's mycelium is a promising therapeutic tool for stimulating the immune response in cancer.

scholarly journals Investigation of Antioxidant and Antimicrobial Activities of Different Extracts of Auricularia and Termitomyces Species of Mushrooms

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Gebreselema Gebreyohannes ◽  
Andrew Nyerere ◽  
Christine Bii ◽  
Desta B. Sbhatu
Keyword(s):  
Antioxidant Activities ◽  
Water Extract ◽  
Antimicrobial Activities ◽  
Hot Water ◽  
Resistant Bacteria ◽  
Scavenging Activity ◽  
Stable Free Radical ◽  
Water Extracts ◽  
Hot Water Extract ◽  
Antioxidant And Antimicrobial Activities

Mushrooms produce a variety of bioactive compounds that are known to have a potential source of antioxidant and antimicrobial properties. Natural antioxidants can protect against free radicals without any side effects. The purpose of this study was to evaluate the antioxidant and antimicrobial activities of Auricularia and Termitomyces extracts. Specimens of Auricularia and Termitomyces spp. were collected from Kakamega National Reserve Forest in Kenya. Specimens were identified, extracted, and screened for their antioxidant and antimicrobial activities using stable free radical DPPH and colorimetric bioassay methods, respectively. The antimicrobial activity of the extracts was tested against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, MRSA, Candida albicans, and Candida parapsilosis. The maximum scavenging activity of hot water extract of Auricularia spp. was observed at 70.4% with the IC50 value of 40 μg/mL. Of the three extracts of Termitomyces spp., 70% ethanol extract has shown the highest scavenging activity (63%) with the IC50 value of 50 μg/mL. Chloroform and hot water extracts of Auricularia have shown statistically significantly different antifungal activities against C. parapsilosis (df = 2, F = 22.49, p ≤ 0.05). Of all the organisms, S. aureus was highly susceptible to 70% ethanol and hot water extracts of Termitomyces spp. with minimum inhibitory concentration values of 0.67±0.29 mg/mL. S. aureus and E. coli were the most susceptible and resistant bacteria to the hot water extract, respectively. In conclusion, the extracts of Auricularia spp. and Termitomyces spp. have shown promising antimicrobial and antioxidant activities.

Antioxidant Properties of Various Extracts from Laoying Tea (Litsea coreana L.)

2011 ◽  
Vol 183-185 ◽  
pp. 45-49
Author(s):  
Hong Fang Ji ◽  
Ling Wen Zhang ◽  
Hai Yan Zhang ◽  
Ming Duo Yang ◽  
Jian Li
Keyword(s):  
Cold Water ◽  
Southern China ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Reducing Power ◽  
Hot Water ◽  
Ferrous Ions ◽  
Water Extracts ◽  
Naturally Occurring ◽  
Chelating Ability

Laoying Tea, leaves of Litsea coreana L., is conventionally consumed as healthy tea beverage in southern China for hundreds of years. The ethanolic, cold water and hot water extracts were prepared and their antioxidant properties studied. At 160 µg/mL, the cold water extract showed a high reducing power of 1.056. With regard to the scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radicals, extracts were effective in the order: ethanolic > cold water > hot water extracts. EC50values for chelating ability on ferrous ions for ethanolic, cold water and hot water extracts were 10.23, 0.85 and 1.12 mg/mL, respectively. Total phenols were the major naturally occurring antioxidant components found and were in the order: cold water > hot water > ethanolic extracts. Based on EC50values, the various extracts from laoying tea were effective antioxidants.

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