scholarly journals Physicochemical Properties and Antimicrobial Activity of Evaporated Extract of Cow Dung Against Some Pathogens

2012 ◽  
Vol 5 (1) ◽  
pp. 135-141
Author(s):  
M. Waziri ◽  
J. S. Suleiman
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Analytical Techniques ◽  
Food Additive ◽  
Cow Dung ◽  
Northern Nigeria ◽  
Elemental Contents ◽  
Mic Value

The evaporated extract of cow dung is traditionally used in Northern Nigeria and Cameroun as food additive and in treatment of infectious diseases. In this study, the cow dung ash extract was prepared and tested for some elemental contents as well as the antimicrobial activity against Cyanobacteria (C.bacteria), Staphylococcus aureus (S.aureus), Bacillus subtilis (B.subtilis) and Escherichia coli (E.coli) using different analytical techniques. The extract was highly basic with pH of 11.7 and the elements vary in the following decreasing order of concentration; K>Na>Mg>Ca>Fe>Al>Zn.  S. aureus was the most sensitive bacteria with minimum inhibitory concentration (MIC) value of 0.082 mg/mL while B. subtilis was the least sensitive with MIC value of 4.3 mg/mL. The result of this study indicate that the extract can supplement the dietary Na and K requirements for the users and supports the folkloric use of the extract in treatment of infections.© 2013 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v5i1.11962        J. Sci. Res. 5 (1), 135-141 (2013)

Antimicrobial Activity of N,N'-bis(decylmethyl)- α,ω-alkanediamine Dioxides [1]

1983 ◽  
Vol 38 (1-2) ◽  
pp. 151-152 ◽  
Author(s):  
Ferdinand Devínsky ◽  
Ivan Lacko ◽  
Ludovít Krasnec ◽  
Dušan Mlynarčík
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Minimal Inhibitory Concentration ◽  
Inhibitory Concentration

Antimicrobial activity of N,N′-bis(decylmethyl)-α,ω-alkanediamine dioxides determined on Staphylococcus aureus, Escherichia coli, and Candida albicans is presented as minimal inhibitory concentration (MIC). The effect of the length of linking alkylene chain on this activity has been followed.

scholarly journals Assessment of antimicrobial activity of Cola milleni seed and pulp extracts against Staphylococcus aureus, Escherichia coli and Penicillium notatum

2020 ◽  
Vol 12 (1) ◽  
pp. 71-75
Author(s):  
A.M. Aliyu ◽  
S.J. Oluwafemi ◽  
S. Kasim
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Bioactive Components ◽  
Zone Of Inhibition ◽  
Penicillium Notatum ◽  
Standard Techniques

All over the world, hundreds of plants have been identified based on researchers and experimental evidence as good sources of medicinal agents. The bioactive components (phytochemicals) of both the seeds and pulp of Cola milleni were extracted using ethanol as solvent. The bioactive components detected were alkaloids, tanins, saponins, cardiac glycosides, carbohydrates, sterols, resins and terpenes while Flavonoids, anthraquinones, anthracyanides and phenol were not detected for both the seed and pulps. Antimicrobial activity of the ethanol extract (Seed and pulp) against Staphylococcus aureus, Escherichia coli and Penicillium notatum was carried out using standard techniques. Staphylococcus aureus had the highest zone of inhibition for pulp having a range of 9.7mm±0.58mm - 19.7mm±2.52mm while Penicllium notatum had the least with 0.00mm. S.aureus also had the highest zone of inhibition range of 14.3mm±2.08mm - 21.3mm±1.53mm for the seed extract while penicillium had the least inhibition range of 5.0mm±1.00mm - 5.7mm±0.58. E.coli showed the highest minimum inhibitory concentration with ethanol extract of the pulp (160mg/ml) while penicillium notatum was not reactive. The minimum inhibitory concentration of seed against penillium notatum was the highest (160mg/ml) while staphylococcus aureus showed the lowest of 40mg/ml. The antimicrobial activity is as a result of the presence of phytochemicals detected, which suggest the use of the plant for the treatment of diseases caused by these organisms. Key words: Cola millenii, Phytochemical, Antimicrobial activity, Bacteria, Fungi

scholarly journals Bio-Guided Fractionation of Prenylated Benzaldehyde Derivatives as Potent Antimicrobial and Antibiofilm from Ammi majus L. Fruits-Associated Aspergillus amstelodami

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4118 ◽  
Author(s):  
Noha Fathallah ◽  
Marwa M. Raafat ◽  
Marwa Y. Issa ◽  
Marwa M. Abdel-Aziz ◽  
Mokhtar Bishr ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Endophytic Fungus ◽  
Inhibitory Concentration ◽  
Antibiofilm Activity ◽  
Moderate Activity ◽  
Ammi Majus ◽  
First Time

Ammi majus L.; Family Apiaceae; is a plant indigenous to Egypt. Its fruits contain bioactive compounds such as furanocoumarins and flavonoids of important biological activities. An endophytic fungus was isolated from the fruits and identified as Aspergillus amstelodami (MK215708) by morphology, microscopical characterization, and molecular identification. To our knowledge this is the first time an endophytic fungus has been isolated from the fruits. The antimicrobial activity of the Ammi majus ethanol fruits extract (AME) and fungal ethyl acetate extract (FEA) were investigated, where the FEA showed higher antimicrobial activity, against all the tested standard strains. Phytochemical investigation of the FEA extract yielded five prenylated benzaldehyde derivative compounds isolated for the first time from this species: Dihydroauroglaucin (1), tetrahydroauroglaucin (2), 2-(3,6-dihydroxyhepta-1,4-dien-1-yl)-3,6-dihydroxy-5-(dimethylallyl)benzaldehyde (3), isotetrahydroauroglaucin )4), and flavoglaucin (5). Structure elucidation was carried out using (1H- and 13C-NMR). Fractions and the major isolated compound 1 were evaluated for their antimicrobial and antibiofilm activity. Compound 1 showed high antimicrobial activity against Escherichia coli with minimum inhibitory concentration (MIC) = 1.95 µg/mL, Streptococcus mutans (MIC = 1.95 µg/mL), and Staphylococcus aureus (MIC = 3.9 µg/mL). It exhibited high antibiofilm activity with minimum biofilm inhibitory concentration (MBIC) = 7.81 µg/mL against Staphylococcus aureus and Escherichia coli biofilms and MBIC = 15.63 µg/mL against Streptococcus mutans and Candida albicans and moderate activity (MBIC = 31.25 µg/mL) against Pseudomonas aeruginosa biofilm. This reveals that dihydroauroglaucin, a prenylated benzaldehyde derivative, has a broad spectrum antimicrobial activity. In conclusion, it was observed that the MICs of the FEA are much lower than that of the AME against all susceptible strains, confirming that the antimicrobial activity of Ammi majus may be due to the ability of its endophytic fungi to produce effective secondary metabolites.

Synthesis and Structures of Halo-Substituted Aroylhydrazones with Antimicrobial Activity

2012 ◽  
Vol 65 (4) ◽  
pp. 343 ◽  
Author(s):  
Mei Zhang ◽  
Dong-Mei Xian ◽  
Hai-Hua Li ◽  
Ji-Cai Zhang ◽  
Zhong-Lu You
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
X Ray Diffraction ◽  
X Ray ◽  
E Coli ◽  
Diphenyl Tetrazolium Bromide

A series of new halo-substituted aroylhydrazones have been prepared and structurally characterized by elemental analysis, 1H NMR, 13C NMR, and IR spectra, and single crystal X-ray diffraction. The compounds were evaluated for their antibacterial (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas fluorescence) and antifungal (Candida albicans and Aspergillus niger) activities by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method. Among the tested compounds, N′-(2-chloro-5-nitrobenzylidene)-2-fluorobenzohydrazide showed the most effective antimicrobial activity with minimum inhibitory concentration values of 0.82, 2.5, 1.7, 15.2, and 37.5 μg mL–1 against B. subtilis, S. aureus, E. coli, P. fluorescence, and C. albicans, respectively. The biological assay indicated that the presence of the electron-withdrawing groups in the aroylhydrazones improved their antimicrobial activities.

scholarly journals FRAKSI ETANOL RIMPANG JERINGAU (Acorus calamus L.) SEBAGAI ANTIBAKTERI RERHADAP Staphylococcus aureus DAN Escherichia coli

1970 ◽  
Author(s):  
Pratika Viogenta ◽  
Nopiyansyah Nopiyansyah ◽  
Fitri Fitri
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Positive Control ◽  
Negative Control ◽  
Acorus Calamus ◽  
Inhibitory Zone ◽  
Plate Method ◽  
Fractionation Process ◽  
Mic Value

Acorus calamus L. (jeringau) rhizomes are one of the medicinal plants used as traditional medicine in Indonesia. This plant were used as an antibacterial. The purpose of this research were to prove the inhibition jeringau rhizome on the selected microorganisme including two bacterial trains Staphylococcus aureus and Escherichia coli. The rhizomes of jeringau were extracted with maceration method using ethanol 70 % and fractionation process using solvent v/v (ethanol, n-hexane and chloroform). The antibacterial activity used the cup plate method with the concentration 20%, 40%, 60%, 80%, and 100%, positive control using ampicillin and negative control using aquades. The test were continved by determining the minimum inhibitor concentration (MIC). Research was obtained zone diameters of 0 mm (negative control), 35.37 mm (positive control), 23.33 mm (concentration 20%), 25.52 mm (40%), 27.11 mm (60%), 30.09 mm (80%), and 33.16 mm (100%) against Staphylococcus aureus. Inhibitory zone diameters 0 mm negative control, 36.68 mm positive control, 22.53 mm (concentration 20%), 25.85 mm (40%), 28.08 mm (60%), 29.73 mm (80%), and 32.21 mm (100%) against Escherichia coli. The minimum inhibitory concentration (MIC) value in both bacterial were 2%. The results showed that all fraction concentrations could inhibit the growth of Staphylococcus aureus and Escherichia coli. Keywords : Rhizome Jeringau, Staphylococcus aureus, Escherichia coli.

scholarly journals Potensi Antimikroba Ekstrak Etanol Kulit Luar Buah Cempedak (Artocarpus integer (Thunb.) Merr.)

2019 ◽  
Vol 5 (1) ◽  
pp. 53-62
Author(s):  
Rina Saputri ◽  
Ali Rakhman Hakim ◽  
Dahlia Syahrina ◽  
Fatthiya Lisyanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Outer Skin ◽  
Biological Source ◽  
Activity Test ◽  
Escherichia Coli Bacteria ◽  
Mic Value

The background of this research is to develop the potential of biological resources in South Kalimantan. In particular, the biological source is cempedak fruit. The use of cempedak which has been scientifically studied is the use of cempedak bark as anti-malaria. Other studies related to other parts of cempedak are still not widely used. Based on the potential possessed by cempedak bark, it is likely that other parts of cempedak also have potential as traditional medicine. This study aims to identify the content of secondary metabolites from the outer skin of cempedak fruit, analyze the potential of the outer skin of cempedak fruit as an antimicrobial and analyze the effective dose as an antimicrobial. The method used to see MIC values with liquid dilution and KBM values with solid dilution. The results of phytochemical identification showed that the ethanol extract of the outer skin of cempedak fruit contained secondary metabolites in the form of saponins, tannins, flavonoids and alkaloids which have antimicrobial potential. The results of the antimicrobial activity test showed the MIC value of the ethanol extract of cempedak fruit outer skin against Escherichia coli bacteria at a concentration of 50% and against Staphylococcus aureus at a concentration of 25%. The KBM value also shows the same value as KHM. Based on the results of the study it can be concluded that the ethanol extract of cempedak fruit outer skin has broad-spectrum antimicrobial activity.

Synthesis of 1ʹ-acetoxychavicol acetate (ACA) analogues and their inhibitory activities against methicillin-resistant Staphylococcus aureus

2020 ◽  
Vol 31 (3) ◽  
pp. 101-111
Author(s):  
Nursyazwani Anuar ◽  
◽  
Mohamad Nurul Azmi Mohamad Taib ◽  
Khayriyyah Mohd Hanafiah ◽  
Aemen Ali Kudayr Al Shammary ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Moderate Activity ◽  
Methicillin Resistant ◽  
Inhibitory Activities ◽  
Inhibitory Agents ◽  
Mic Value

A series of 1ʹ-acetoxychavicol acetate analogues were synthesised and evaluated for their antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) using broth microdilution technique. The minimum inhibitory concentration (MIC) was used to determine whether the compounds had potential as inhibitory agents against the MRSA ATCC 43300, and the compounds with antimicrobial potential (<2000 μg ml–1) were tested for minimum bactericidal concentration (MBC). Based on this assay, compound 1 exhibited potent antimicrobial activity with MIC value of 250 μg ml–1. Meanwhile, compounds 2 and 13 showed the moderate activity with MIC values of 500 μg ml–1, respectively.

CHEMICAL CONSTITUENTS AND IN-VITRO ANTIMICROBIAL ACTIVITY OF POLYALTHIA STENOPETALA ESSENTIAL OIL

2016 ◽  
Vol 78 (6-8) ◽  
Author(s):  
Noor Izzatie Munira Kamaruddin ◽  
Nor Azah Mohamad Ali ◽  
Muhd Fauzi Safian ◽  
Zaidah Zainal Ariffin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc

Polyalthia stenopetala essential oils were extracted by hydrodistillation from the leaves and were analyzed using gas chromatography-flame ionization detector (GC-FID) and gas chromatography/mass spectrometry (GC/MS) system. Thirty one compounds were identified from the analysis. The most abundant components in the leaves oil are curzerene (37.56%) followed by viridiflorol (11.59%), germacrene B (3.77%) and aromadendrene (4.01%). The antimicrobial activity of the oil essential oils was determined with disk diffusion method and minimum inhibitory concentration (MIC) assay. Four bacteria, Staphylococcus aureus (ATCC 25923), Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC 25922) Psedomonas aeruginosa (ATCC 10145) and two yeasts, Candida albicans (ATCC 10231) and Saccharomyces cerevisiae (ATCC 4098) were selected. The crude oil shows the most reactivity against B. subtilis (ATCC 6633) and C. albicans (ATCC 10231) with an inhibitory zone of 11mm. The minimum inhibitory concentration (MIC) of sample against Staphylococcus aureus (ATCC 25923), Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC 25922) Psedomonas aeruginosa (ATCC 10145) and Candida albicans (ATCC 10231) in range of 0.5 mg/ml – 1.0 mg/ml which can categorized as strong.

scholarly journals Antimicrobial Activity of the Dichloromethane Extract from in vitro Cultured Roots of Morinda royoc and Its Main Constituents

2010 ◽  
Vol 5 (5) ◽  
pp. 1934578X1000500
Author(s):  
Janetsy Borroto ◽  
Ricardo Salazar ◽  
Alejandro Pérez ◽  
Yemeys Quiros ◽  
Martha Hernandez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Bacterial Strains ◽  
Yeast Strains ◽  
Mic Value

The dichloromethane extract and seven anthraquinones isolated from in vitro cultured roots of Morinda royoc L. were tested for their antimicrobial activity against seven yeast and seven bacterial strains. The extract showed a minimum inhibitory concentration (MIC) value of 15.6 μg/m against all species of Candida tested; except C. glabrata (MIC 1.95 μg/mL), and it inhibited the growth of oxacillin-resistant Staphylococcus aureus (MIC 31.2 μg/mL). Only morindone showed activity against all yeast strains (MIC 1.9 μg/mL), and against oxacillin-resistant Staphylococcus aureus (MIC 15 μg/mL).

scholarly journals Purpurin Esters Containing a Saturated Cyclic Fragment with Antimicrobial Activity

2020 ◽  
Vol 22 (1) ◽  
pp. 43 ◽  
Author(s):  
Т.V. Kharlamova ◽  
R.B. Seidakhmetova ◽  
K.D. Praliyev
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Acid Chlorides ◽  
Bacillus Subtilis Atcc ◽  
Cyclic Fragment ◽  
Mic Value

The present study is a continuation of the scientific research works for the synthesis of anthraquinonе-containing derivatives with cyclic ring systems by the interaction of 1,2,4-trihydroxyanthraquinone (purpurin) with cyclic carboxylic acid chlorides. Series of purpurin esters containing a saturated cyclic fragment was studied for antibacterial activity about museum strains of microorganisms. The effects of these preparations in vitro about Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Pseudomonas aeruginosa АТСС 27853 and fungus Сandida albicans АТСС 10231 were studied. It has been found that the compounds with the cyclohexane fragment have possess an antimicrobial activity with strains of microorganisms Staphylococcus aureus АТСС 6538 for which the MIC value was 31.25 μg/ml.

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