scholarly journals Chemical composition and antimicrobial activity of leaves essential oil of Juniperus communis (Cupressaceae) grown in Republic of Macedonia

2013 ◽  
Vol 59 ◽  
pp. 23-32 ◽  
Author(s):  
Floresha Sela ◽  
Marija Karapandzova ◽  
Gjose Stefkov ◽  
Ivana Cvetkovikj ◽  
Elena Trajkovska-Dokik ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Salmonella Enteritidis ◽  
Shigella Flexneri ◽  
Dilution Method ◽  
Klebsiella Pneumonia ◽  
Juniperus Communis ◽  
Acinetobacter Spp

Chemical composition and antimicrobial activity of essential oils isolated from leaves of three different samples of wild growing Juniperus communis L. (Cupressaceae) from R. Macedonia was investigated. Essential oil yield ranged from 7.3 to 9.0 ml/kg. Performing GC/ FID/MS analysis, ninety components were identified, representing 86.07-93.31% of the oil. The major components of the leaves essential oil (LEO) were α-pinene (21.37-28.68%) and sabinene (2.29-16.27%), followed by limonene, terpinen-4-ol, β-elemene, trans-(E)-caryophyllene, germacrene D and δ-cadinene. Antimicrobial screening of the LEO was made by disc diffusion and broth dilution method against 16 bacterial isolates of Gram positive and Gram negative bacteria and one strain of Candida albicans. Two bacteria, Staphylococcus aureus and Streptococcus pyogenes were sensitive to antimicrobial activity of LEO (MIC = 125 µl/ml). Additionally, LEO showed moderate antimicrobial activity against Streptococcus agalactiae, Haemophilus influnzae, Corynebacterium spp. and Campylobacter jejuni (MIC > 500 µl/ml). Candida albicans, Staphylococcus epidermidis, Acinetobacter spp., Salmonella enteritidis, Shigella flexneri, Klebsiella pneumonia, Pseudomonas aeruginosa and Proteus mirabilis were completely resistant to the antimicrobial effects of this.

scholarly journals Chemical composition and antimicrobial activity of berry essential oil of Juniperus oxycedrus L. (Cupressaceae) grown wild in Republic of Macedonia

2013 ◽  
Vol 59 ◽  
pp. 41-48 ◽  
Author(s):  
Floresha Sela ◽  
Marija Karapandzova ◽  
Gjose Stefkov ◽  
Ivana Cvetkovikj ◽  
Elena Trajkovska-Dokikj ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Salmonella Enteritidis ◽  
Dilution Method ◽  
Klebsiella Pneumonia ◽  
Juniperus Oxycedrus ◽  
Republic Of Macedonia

Chemical composition and antimicrobial activity of essential oil isolated from berries from 2 different samples of Juniperus oxycedrus L. (Cupressaceae), growing wild in Republic of Macedonia was investigated. Performing GC/FID/MS analysis, one hundred components were identified, representing 96.0-98.95% of the oil. The major components were α-pinene (22.54- 27.12%), myrcene (11.26- 15.13%) and limonene (2.78-18.06%). Antimicrobial screening of the J. oxycedrus essential oils was made by disc diffusion and broth dilution method against 16 bacterial isolates of Gram positive and Gram negative bacteria and one strain of Candida albicans. The most sensitive bacteria was Haemophilus influenzae (MIC = 125 ml/ml). The essential oils showed moderate antimicrobial activity against Streptococcus pneumoniae, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pyogenes, Corynebacterium spp., Escherichia coli and Campilobacter jejuni (MIC > 500 ml/ml) and no activity against Candida albicans, Staphylococcus epidermidis, Acinetobacter spp., Salmonella enteritidis, Shigella flexnery, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterococcus and Proteus mirabilis.

scholarly journals In vitro Antimicrobial Properties and Chemical Composition of Santolina chamaecyparissus Essential Oil from Algeria

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Samah Djeddi ◽  
Khadidja Djebile ◽  
Ghania Hadjbourega ◽  
Zoubida Achour ◽  
Catherine Argyropoulou ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Klebsiella Pneumonia ◽  
Aerial Parts

The chemical composition of the essential oil obtained from the aerial parts of Santolina chamaecyparissus L., growing in Algeria, was investigated by GC-MS analyses. A total of 36 compounds were identified, accounting for 91.7% of the essential oil obtained. Camphor (31.1%) and cubenol (17.0%) were the predominant compounds. The potential of the antimicrobial activity was also investigated and the tested sample proved to be very active against Klebsiella pneumonia and Candida albicans (34.1 ± 0.02 mm and 35.0 ± 0.01 mm, respectively). Transverse sections of the leaf and stem of the plant suggest that the essential oil is localized in endogenous and exogenous sites.

scholarly journals Chemical composition and antimicrobial activity of the essential oil from the leaves and flowers of Aloysia gratissima

2013 ◽  
Vol 15 (4) ◽  
pp. 583-588 ◽  
Author(s):  
F.M. Santos ◽  
J.E.B.P. Pinto ◽  
S.K.V. Bertolucci ◽  
A.A. Alvarenga ◽  
M.N. Alves ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Dilution Method ◽  
Volatile Oils ◽  
Salmonella Choleraesuis ◽  
High Concentrations ◽  
Type Apparatus

Volatile oils from leaves and flowers of Aloysia gratissima were investigated for their chemical composition and antimicrobial activity against the bacteria Bacilus subtilis, Staphylococcus aureus, Salmonella choleraesuis, Pseudomonas aeruginosa, Streptococcus pneumoniae and the Candida albicans yeast. The minimum inhibitory concentrations (MIC) of the oils were determined by the micro-dilution method, while the chemical composition was determined by GC-MS (gas chromatography mass spectrometry). The fresh leaves and inflorescence were subjected to hydrodistillation for 120 min using a Clevenger-type apparatus, and the essential oil was tested against microorganisms. High concentrations of sesquiterpenes were observed for the inflorescence, and monoterpenes were observed for the leaves. The main compounds of the inflorescence essential oil were E-caryophyllene, germacrene B, guaiol and bulnesol, while in the leaves the main compounds were trans-pinocamphone, trans-pinocarveyl acetate, and guaiol. The essential oil from the leaves showed an effect against P. aeruginosa and S. pneumonia, and the essential oil of the inflorescence showed an effect against P. aeruginosa, S. pneumonia, and Candida albicans.

scholarly journals Chemical Composition and Preliminary Antimicrobial Activity of the Hydroxylated Sesquiterpenes in the Essential Oil from Piper barbatum Kunth Leaves

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 211 ◽  
Author(s):  
Paco Noriega ◽  
José Ballesteros ◽  
Alejandra De la Cruz ◽  
Tatiana Veloz
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Forest Ecosystem ◽  
Raw Materials ◽  
The Andes ◽  
Ancestral Knowledge ◽  
Antifungal Potential

This study evaluates the antimicrobial and antifungal potential of the essential oil extracted from a species located in the Andes of Ecuador, Piper barbatum Kunth, known as “cordoncillo” or “allupa”, used by the Quichua people as an antibacterial plant for washing female genitalia in cases of infection. The most abundant molecules in the essential oil were: α- phellandrene (43.16%), limonene (7.04%); some oxygenated sesquiterpenes such as: trans-sesquisabinene hydrate (8.23%), elemol (7.21%) and others. The evaluation of antimicrobial activity showed activity in all the strains analyzed; however, those in which MIC values are considered to be very strong (less than 500 µg/mL) are: Staphylococcus aureus 264 µg/mL, Streptococcus mutans 132 µg/mL, Candida albicans 132 µg/mL and Candida tropicalis 264 µg/mL. Antimicrobial bioautography defines which molecules are responsible for the activity; thus, it was possible to establish the chromatographic regions of = 0.02 and Rf = 0.04, as those with active molecules. It was established that 4 hydroxylated sesquiterpene molecules are involved: elemol (7.21%), trans-sesquisabinene hydrate (8.23%), β–eudesmol (3.49%) and 10-epi-γ-eudesmol (1.07%); the last two being the most active. The aim of this manuscript is to analyze both the ancestral knowledge of the Quichua people of Ecuador, and the chemical-biodiversity of the Andean forest ecosystem, in order to provide new raw materials of pharmaceutical interest.

scholarly journals Antimicrobial Activity and Chemical Composition of the Essential Oils of Portuguese Foeniculum vulgare Fruits

2015 ◽  
Vol 10 (4) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Ana S. Mota ◽  
M. Rosário Martins ◽  
Sílvia Arantes ◽  
Violeta R. Lopes ◽  
Eliseu Bettencourt ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Salmonella Enteritidis ◽  
Gas Chromatography Mass Spectrometry ◽  
Dilution Method ◽  
Foeniculum Vulgare ◽  
Serovar Typhimurium ◽  
Food Spoilage Fungi

The aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from fruits of six fennel accessions collected from wild populations occurring in the centre and south of Portugal. Composition of essential oils was established by Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The obtained yields of the essential oils were found to vary greatly in the range of 1.1 to 2.9% (v/w) and the chemical composition varied with the region of collection. A total of 16 compounds were identified. The main compounds were fenchone (16.9 – 34.7%), estragole (2.5 – 66.0%) and trans-anethole (7.9 – 77.7%). The percentages of these three main compounds were used to determine the relationship between the different oil samples and to group them into four different chemotypes: anethole/fenchone; anethole; estragole and anethole/estragole. Antifungal activity of essential oils was evaluated against six food spoilage fungi: Aspergillus niger, A. japonicus, A. oryzae, Fusarium oxysporum, Rhizophus oryzae and R. stolonifer. Antibacterial activity was assessed against three Gram-positive strains: Enterococcus faecalis ATCC 29212, Staphylococcus epidermidis ATCC 12228 and S. aureus ATCC 28213; and against six Gram-negative strains: Escherichia coli ATCC 25922; Morganella morganii LFG 08; Proteus mirabilis LFG 04; Salmonella enteritidis LFG 05; S. entiritidis serovar typhimurium LFG 06 and Pseudomonas aeruginosa ATCC 27853 by the disc diffusion agar method; the minimal inhibitory concentration (MIC) was determined using the broth macro-dilution method. The MIC values varied from 62.5 ( E. coli ATCC 25922) to 2000 μg/mL ( P. aeruginosa ATCC 27853).

scholarly journals Essential Oil Composition and Antimicrobial Activity of Aerial Parts and Ripe Fruits of Echinophora spinosa (Apiaceae) from Italy

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800
Author(s):  
Daniele Fraternale ◽  
Salvatore Genovese ◽  
Donata Ricci
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Intestinal Microflora ◽  
Central Italy ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Aerial Parts ◽  
Eubacterium Limosum

The chemical composition and antimicrobial activity of the essential oils obtained from the flowering aerial parts and ripe fruits of Echinophora spinosa L. (Apiaceae) from central Italy were analyzed by GC/MS. The major constituents of the oil from the aerial parts were β-phellandrene (34.7%), myristicin (16.5%), δ3-carene (12.6%), α-pinene (6.7%) and α-phellandrene (6.2%), and of the oil from the ripe fruits p-cymene (50.2%), myristicin (15.3%), α-pinene (15.1%) and α-phellandrene (8.1%). The two oils showed good antimicrobial activity against Clostridium difficile, C perfringens, Enterococcus faecalis, Eubacterium limosum, Peptostreptococcus anaerobius and Candida albicans with MIC values respectively of 0.25, 0.25, 0.25, 0.25, 2.25, and 0.50%, v/v, and 0.13, 0.13, 0.13, 0.13, 2.25, 0.50%, v/v, for aerial parts and ripe fruits respectively. A less significant antimicrobial activity against bifidobacteria and lactobacilli, very important in the intestinal microflora, was also detected, with MIC values higher than 4.0%, v/v.

scholarly journals Antimicrobial activity of essential oils of Juniperus phoenicea from North Western Algeria

2017 ◽  
Vol 1 ◽  
pp. 01
Author(s):  
G. Bachir Raho ◽  
M. Otsmane ◽  
F. Sebaa
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Essential Oils ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Klebsiella Pneumonia ◽  
Variable Degree ◽  
Juniperus Phoenicea ◽  
Test Organisms ◽  
Western Algeria

Juniperus phoenicea (Family: Cupressaceae) is an evergreen tree widely distributed in North Africa including Algeria. The aim of this investigation was to analyse the antimicrobial potential of essential oils from J. phoenicea on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Streptococcus sp, Bacillus sp and Candida albicans using wells and discs diffusion methods. Broth dilution method was utilized to study the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). The results showed a variable degree of antimicrobial activity. The diameters of inhibition zones for all test organisms were in the ranges of 7–21 mm, while MIC was from 62.5 to >500µl/ml and MBC from 250 to >500µl/ml. The highest antimicrobial activities were observed against Gram positive bacteria followed by Gram negative ones then Candida albicans. The findings provide the evidence that J. phoenicea as a good medicinal plant for further investigations. 

scholarly journals Chemical Composition of Nardostachys grandiflora Rhizome Oil from Nepal – A Contribution to the Chemotaxonomy and Bioactivity of Nardostachys

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Prabodh Satyal ◽  
Bhuwan K. Chhetri ◽  
Noura S. Dosoky ◽  
Ambika Poudel ◽  
William N. Setzer
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Cytotoxic Activity ◽  
Biological Activities ◽  
Mcf 7

The essential oil from the dried rhizome of Nardostachys grandiflora, collected from Jaljale, Nepal, was obtained in 1.4% yield, and a total of 72 compounds were identified constituting 93.8% of the essential oil. The rhizome essential oil of N. grandiflora was mostly composed of calarene (9.4%), valerena-4,7(11)-diene (7.1%), nardol A (6.0%), 1(10)-aristolen-9-ol (11.6%), jatamansone (7.9%), valeranal (5.6%), and cis-valerinic acid (5.7%). The chemical composition of N. grandiflora rhizome oil from Nepal is qualitatively very different than those from Indian, Chinese, and Pakistani Nardostachys essential oils. In this study we have evaluated the chemical composition and biological activities of N. grandiflora from Nepal. Additionally, 1(10)-aristolen-9-ol was isolated and the structure determined by NMR, and represents the first report of this compound from N. grandiflora. N. grandiflora rhizome oil showed in-vitro antimicrobial activity against Bacillus cereus, Escherichia coli, and Candida albicans (MIC = 156 μg/mL), as well as in-vitro cytotoxic activity on MCF-7 cells.

scholarly journals Chemical Composition and Antimicrobial Activity of Essential Oil of Heracleum rigens

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Nataraj Jagannath ◽  
Hanumanthaiah Ramakrishnaiah ◽  
Venkatarangaiah Krishna ◽  
Prameela Javarayi Gowda
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Bornyl Acetate ◽  
Klebsiella Pneumonia ◽  
Oil Composition ◽  
Bacterial Strains ◽  
Octyl Acetate ◽  
Total Oil

The essential oil was extracted from the seeds of Heracleum rigens by hydrodistillation and a total of twenty compounds accounting for 98.5% of the total oil composition were identified. Physicochemical properties and chemical composition of the oil was determined by a combination GC/FID and GC/MS analysis. The major compounds identified were bornyl acetate (51.2%), α-pinene (22.6%), limonene (9.62%), octyl acetate (3.94%), p-cymene (2.85%) and γ-terpinene (1.93%). The antimicrobial activity of the oil was screened by the disc diffusion method against nine pathogenic bacterial strains. Maximum antimicrobial activity was noted against Klebsiella pneumonia and Bacillus subtillis. This investigation corroborates the traditional claim of H. rigens as an effective antimicrobial agent.

scholarly journals Chemical Composition and Antimicrobial Activity of the Essential Oils of Pinus peuce (Pinaceae) Growing Wild in R. Macedonia

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Marija Karapandzova ◽  
Gjose Stefkov ◽  
Ivana Cvetkovikj ◽  
Elena Trajkovska-Dokik ◽  
Ana Kaftandzieva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Haemophilus Influenzae ◽  
Essential Oils ◽  
Streptococcus Agalactiae ◽  
Bornyl Acetate ◽  
Acinetobacter Spp ◽  
Pinus Peuce

The chemical composition and antimicrobial activity of the essential oils isolated from twigs with needles (T+N) and from twigs without needles (T-N) from wild Pinus peuce Griseb. (Pinaceae), from three different locations in R. Macedonia, were investigated. Essential oil yields of T+N ranged from 7.5 mL/kg to 12.5 mL/kg and for T-N from 13.8 mL/kg to 17.3 mL/kg. GC/FID/MS analysis of the essential oils revealed eighty-four components, representing 93.7–95.7% and 91.2–92.0% of the T+N and T-N oils, respectively. The major components in T+N and T-N oils were monoterpenes: α-pinene (23.8–39.9%, 21.2–23.3%), camphene (2.2–5.5%, 0.7–2.0%), β-pinene (10.1–17.1%, 8.2–16.4%), myrcene (1.2–1.41%, 1.6–2.5%), limonene+β-phellandrene (6.8–14.0%, 8.8–23.6%) and bornyl acetate (2.3–6.9%, 1.1–3.4%), followed by the sesquiterpenes: trans-( E)-caryophyllene (3.6–4.3%, 3.2–7.3%), germacrene D (7.1–9.5%, 5.0–10.3%) and δ-cadinene (2.1–3.1%, 3.3–4.2%, respectively). Antimicrobial screening of the essential oils was made by disk diffusion and broth dilution methods against 13 bacterial isolates of Gram-positive and Gram-negative bacteria and one strain of Candida albicans. T-N essential oils showed antimicrobial activity toward Streptococcus pneumoniae, Staphylococcus aureus, S. epidermidis and Candida albicans as well as Streptococcus agalactiae, Acinetobacter spp. and Haemophilus influenzae. The antimicrobial activity of T+N essential oils was greater, especially against Streptococcus agalactiae, S. pyogenes, Enterococcus and Candida albicans, followed by Haemophilus influenzae, Acinetobacter spp., Escherichia coli, Salmonella enteritidis, Staphylococcus aureus and S. epidermidis. Minimal inhibitory concentrations (MICs) of all tested essential oils ranged from 15–125 μL/mL. Summarizing the obtained results, the antimicrobial activity of Pinus peuce T+N and T-N essential oils collected from different localities in R. Macedonia varied considerably. These alterations in the antimicrobial activity can be attributed to the differences in the quantitative composition and percentage amounts of the components present in the respective essential oils, although it was evident that there were no differences in the qualitative composition of the essential oils, regardless of the locality of collection, or the type of plant material (T+N or T-N).

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