Antimicrobial activity of berries and leaves essential oils of Macedonian Juniperus foetidissima Willd. (Cupressaceae)

Chemical composition and antimicrobial activity of leaves and berries essential oils from Juniperus foetidissima Willd. (Cupressaceae) grown in R. Macedonia (RM) was investigated. GC/FID/MS analysis was carried out and 93 components were identified, representing 89.7-96.5% of the oils. The major components of the berries essential oil were α-pinene (19.2%), limonene (24.9%) and cedrol (23.1%), followed by smaller amounts of b-funebrene, trans-caryophyllene, germacrene D and d-cadinene. The composition of the leaves essential oil was variable depending on the region of collection. Accordingly, samples originated from southeastern RM contained essential oil with α-pinene (67.6%) and limonene (10.0%), from central part of RM with limonene (17.9-27.1%) and cedrol (28.8-33.9%), while samples from southwestern RM contained oil with terpinen-4-ol (19.1%), cis-thujone (8.3%), germacrene D (11.0%) and d-cadinene (6.3%) as predominant components in the oil. Antimicrobial screening of the essential oils was made by disc diffusion and broth dilution method against 16 bacterial strains of Gram-positive and Gram-negative bacteria and one strain of Candida albicans. The leaves essential oil showed stronger antimicrobial activity against Staphylococcus aureus, Streptococcus pyogenes and Haemophilus influenzae (MIC = 125 ml/ml) and moderate activity against Campylobacter jejuni (MIC > 500 ml/ml). Other investigated bacterial strains and Candida albicans were completely resistant to the antimicrobial activity of J. foetidissima essential oils.

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Antimicrobial Activity and Chemical Analysis of the Essential Oil of Algerian Juniperus phoenicea

Natural Product Communications ◽

10.1177/1934578x1601100426 ◽

2016 ◽

Vol 11 (4) ◽

pp. 1934578X1601100 ◽

Cited By ~ 3

Author(s):

Ahmed Bouyahyaoui ◽

Fouad Bahri ◽

Abderrahmane Romane ◽

Martina Höferl ◽

Juergen Wanner ◽

...

Keyword(s):

Candida Albicans ◽

Essential Oil ◽

Essential Oils ◽

Bacterial Strains ◽

Highly Active ◽

Juniperus Phoenicea ◽

Germacrene D ◽

Antifungal Substances ◽

Total Composition ◽

Hospital Acquired

The essential oils of Juniperus phoenicea L. from Algeria were obtained by hydrodistillation and analyzed by GC-FID and GC-MS. Concerning their chemical composition, 74, 61 and 72 volatile compounds were identified from fresh leaves, dried leaves and berries, representing 88.8%, 91.3% and 94.7% of the total composition, respectively. The main monoterpene in the oils of fresh leaves, dried leaves and berries was α-pinene (29.6% / 55.9% / 56.6%), accompanied by lesser amounts of the sesquiterpenes β-caryophyllene (2.6% / 1.6% /1.2%) and germacrene D (2.01% / 1.7% / 1.5%), respectively. Antibacterial activity of J. phoenicea essential oils was tested against one Gram-negative and four Gram-positive bacterial strains and the yeast Candida albicans, responsible for nosocomial infections. As references, 14 antibiotics and 5 antifungal agents were evaluated. The berry essential oil was ineffective against all but two of the strains tested, whereas the essential oil of dried leaves significantly inhibited all strains but Pseudomonas aeruginosa, which turned out to be the most resistant strain overall. However, Escherichia coli was the most susceptible to the essential oils tested. The essential oil of dry leaves was highly active against Candida albicans, outclassing even the standard antifungal substances. These promising results could substantiate the use of essential oils in the treatment of hospital-acquired infections.

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Chemical composition and antimicrobial activity of berry essential oil of Juniperus oxycedrus L. (Cupressaceae) grown wild in Republic of Macedonia

Macedonian Pharmaceutical Bulletin ◽

10.33320/maced.pharm.bull.2013.59.005 ◽

2013 ◽

Vol 59 ◽

pp. 41-48 ◽

Cited By ~ 1

Author(s):

Floresha Sela ◽

Marija Karapandzova ◽

Gjose Stefkov ◽

Ivana Cvetkovikj ◽

Elena Trajkovska-Dokikj ◽

...

Keyword(s):

Antimicrobial Activity ◽

Candida Albicans ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Salmonella Enteritidis ◽

Dilution Method ◽

Klebsiella Pneumonia ◽

Juniperus Oxycedrus ◽

Republic Of Macedonia

Chemical composition and antimicrobial activity of essential oil isolated from berries from 2 different samples of Juniperus oxycedrus L. (Cupressaceae), growing wild in Republic of Macedonia was investigated. Performing GC/FID/MS analysis, one hundred components were identified, representing 96.0-98.95% of the oil. The major components were α-pinene (22.54- 27.12%), myrcene (11.26- 15.13%) and limonene (2.78-18.06%). Antimicrobial screening of the J. oxycedrus essential oils was made by disc diffusion and broth dilution method against 16 bacterial isolates of Gram positive and Gram negative bacteria and one strain of Candida albicans. The most sensitive bacteria was Haemophilus influenzae (MIC = 125 ml/ml). The essential oils showed moderate antimicrobial activity against Streptococcus pneumoniae, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pyogenes, Corynebacterium spp., Escherichia coli and Campilobacter jejuni (MIC > 500 ml/ml) and no activity against Candida albicans, Staphylococcus epidermidis, Acinetobacter spp., Salmonella enteritidis, Shigella flexnery, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterococcus and Proteus mirabilis.

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Chemical Composition, Antioxidant and Antibacterial Activity of Bunium persicum, Eucalyptus globulus, and Rose Water on Multidrug-Resistant Listeria Species

Journal of Evidence-Based Integrative Medicine ◽

10.1177/2515690x17751314 ◽

2018 ◽

Vol 23 ◽

pp. 2515690X1775131 ◽

Cited By ~ 7

Author(s):

Farhad Sharafati Chaleshtori ◽

Mohamad Saholi ◽

Reza Sharafati Chaleshtori

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Eucalyptus Globulus ◽

Multidrug Resistant ◽

Bacterial Strains ◽

Antioxidant Power ◽

Listeria Species ◽

Bunium Persicum

This research was aimed at investigating the antioxidant and antibacterial activity of Bunium persicum, Eucalyptus globulus, and rose water on multidrug-resistant Listeria species. The antibiotic resistance of Listeria spp obtained from seafood samples were determined by the Kirby-Bauer method. The antioxidant and antibacterial activity of the essential oils and extracts were evaluated using ferric reducing antioxidant power and microdilution methods, respectively. A total 2 samples (1.88%) were positive for Listeria spp. L monocytogenes was found to be resistant to ampicillin, amoxicillin/clavulanic acid, penicillin, vancomycin, and kanamycin. B persicum essential oil showed the greatest antioxidant activity (248.56 ± 1.09 µM Fe2+/g). The E globulus essential oil showed consistently strong antimicrobial activity against L monocytogenes and L grayi, while rose water showed no antimicrobial activity against any of the tested bacterial strains. The results showed that after adding the B persicum and E globulus essential oils to bacteria, the cell components’ release increased significantly.

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GC/MS Analysis and Antimicrobial Activity of the Essential Oil of Fresh Leaves of Eucalytus Globulus, and Leaves and Stems of Smyrnium Olusatrum from Constantine (Algeria)

Natural Product Communications ◽

10.1177/1934578x1000501031 ◽

2010 ◽

Vol 5 (10) ◽

pp. 1934578X1000501 ◽

Cited By ~ 5

Author(s):

Habiba Daroui-Mokaddem ◽

Ahmed Kabouche ◽

Mabrouka Bouacha ◽

Boudjemaa Soumati ◽

Aida El-Azzouny ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Eucalyptus Globulus ◽

Bacterial Strains ◽

Ms Analysis ◽

Wide Range ◽

Main Components ◽

Type Apparatus

The essential oils of fresh leaves of Eucalyptus globulus L. (Myrtaceae), and leaves and stems of Smyrnium olusatrum L. (Apiaceae), obtained by hydrodistillation in a Clevenger-type apparatus, were analyzed by GC/MS. Twenty compounds were characterized, representing 98.3% of the essential oil of E. globulus, with 1,8-cineole (48.6%), globulol (10.9%), trans-pinocarveol (10.7%), and α-terpineol (6.6%) as main components, while thirty-three compounds were characterized, representing 94.3% of the essential oil of S. olusatrum, with sabinene (27.1%), curzerene (13.7%), methyl-1-benzyl-2-oxocyclooctane carboxylate (12.3%), α-pinene (7.2%), cryptone (7.1%) and β-pinene (5.7%) as the major components. The essential oils of E. globulus and S. olusatrum were tested against a wide range of fungal and bacterial strains. Both oils showed significant antimicrobial activity.

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Synergistic Effects of Essential Oils of Ammoides verticillata and Satureja candidissima Against Many Pathogenic Microorganisms

Anti-Infective Agents ◽

10.2174/2211352517666190227161811 ◽

2020 ◽

Vol 18 (1) ◽

pp. 72-78 ◽

Cited By ~ 3

Author(s):

Fatima Benyoucef ◽

Mohammed El Amine Dib ◽

Boufeldja Tabti ◽

Arrar Zoheir ◽

Jean Costa ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Synergistic Effects ◽

Lactobacillus Rhamnosus ◽

Salmonella Typhi ◽

Antimicrobial Activities ◽

Bacterial Strains ◽

Gram Negative ◽

Ammoides Verticillata

Background: Antibiotic resistance is today one of the most serious threats to global health, food security and development. Due to the growing number of infections, treatment becomes more difficult, if not impossible, because of the loss of antibiotic efficacy. Objective: In the present investigation, the chemical composition of essential oils of Ammoides verticillata and Satureja candidissima and their synergistic effects on antimicrobial activities were investigated. Methods: The chemical composition of the essential oil was analyzed by Gas Chromatography (GC) and Gas Chromatography-Mass Spectroscopy (GC/MS). The antimicrobial activity of the essential oils was investigated using dilution-agar method against nine bacterial strains three Gram-negative Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Salmonella typhi (ATCC 14028), and six Gram-positive: Staphylococcus aureus (ATCC 43300,) Clostridium sporogenes (ATCC 19404), Bacillus subtilis (ATCC 6633), Enterococcus faecalis (ATCC 7314), Lactobacillus rhamnosus (ATCC 53103) and Bacillus cereus (ATCC 14579). Results: The essential oil of A. verticillata was characterized principally by carvacrol (44,3%), Limonene (19,3%) and p-cymene (19,2%). The constituents identified of S. candidissima essential oil were principally oxygenated monoterpenes represented by pulegone (70,4%). The essential oil of A. verticillata had a good antimicrobial activity against four bacterial strains (Escherichia coli, Salmonella typhi, Lactobacillus rhamnosus and Bacillus cereus) with MIC and MBC values between 0.2-0.4 µl/ml and 0.2-6.2 µl/ml, respectively. While, S. candidissima essential oil had moderate antimicrobial activities against all strains with MIC and MBC values between 1.5-6.2 µl/ml and 6.2-12.5 µl/ml, respectively. The results of antimicrobial activity of essential oils blend presented higher antimicrobial activity against all tested bacteria with MIC and MBC values between 0.3-1.5 µl/ml and 0.4-6.2 µl/ml, respectively. Conclusion: The essential oils blend presented high antimicrobial activity compared to virgin oils. This activity can be due to the association of active compounds such as carvacrol and pulegone. These findings provide a new source of drugs that may help in therapy to lead to the development of a new treatment based on a combination of these essential oils against gram-negative and gram-positive bacteria that continue to pose a threat to public health.

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Constituents and antimicrobial activity of the essential oils of six Himalayan Nepeta species

Journal of the Serbian Chemical Society ◽

10.2298/jsc091106052b ◽

2010 ◽

Vol 75 (6) ◽

pp. 739-747 ◽

Cited By ~ 27

Author(s):

Dinesh Bisht ◽

Rajendra Padalia ◽

Lalit Singh ◽

Veena Pande ◽

Priyanka Lal ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Antagonistic Activity ◽

Significant Activity ◽

Variable Degree ◽

Bacterial Strains ◽

Fungal Strains ◽

Microbial Strains

The essential oils from six Himalayan Nepeta species, viz. Nepeta leucophylla Benth., Nepeta discolor Royle ex Benth., Nepeta govaniana Benth., Nepeta clarkei Hook f., Nepeta elliptica Royle ex Benth. and Nepeta erecta Benth., were tested for their in vitro antimicrobial activity against six pathogenic bacterial and two fungal strains. The results showed that Pseudomonas aeruginosa was the most sensitive strain tested to the essential oils of Nepeta species. The essential oils of N. elliptica and N. erecta exhibited the highest activity against P. aeruginosa, followed by the essential oils of N. leucophylla and N. clarkei. The essential oils from N. elliptica and N. erecta were also found to be very effective against Serratia marcescens; while the essential oil from N. leucophylla displayed significant activity against Proteus vulgaris and Staphylococcus aureus. Other bacterial strains displayed variable degree of susceptibility against one or more of the tested essential oils. The essential oil from N. leucophylla also showed the highest antifungal activity against both tested fungal strains, viz. Candida albicans and Trichophyton rubrum, followed by the essential oils from N. clarkei, N. govaniana and N. erecta. Iridodial derivatives, viz. iridodial ?-monoenol acetate (25.4 %), dihydroiridodial diacetate (18.2 %) and iridodial dienol diacetate (7.8 %) were identified as the major constituents of N. leucophylla, while the essential oils from N. elliptica and N. erecta were dominated by (7R)-trans, trans nepetalactone (83.4 %) and isoiridomyrmecin (66.7 %), respectively. The essential oil of N. discolor was characterized by 1,8-cineole (25.5 %) and ?-caryophyllene (18.6 %), while N. clarkei was dominated by ?-sesquiphellandrene (22.0 %) and germacrene D (13.0 %). Isoiridomyrmecin (35.2 %) and pregeijerene (20.7 %) were identified as the major constituents of N. govaniana. In general the Nepeta species containing constituents with an iridoid or lactone skeleton were found to have the greater antagonistic activity against most of the microbial strains as compared to those containing regular terpene constituents.

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Antimicrobial activity of essential oils of Juniperus phoenicea from North Western Algeria

Journal of Medicinal Botany ◽

10.25081/jmb.2017.v1.41 ◽

2017 ◽

Vol 1 ◽

pp. 01

Author(s):

G. Bachir Raho ◽

M. Otsmane ◽

F. Sebaa

Keyword(s):

Antimicrobial Activity ◽

Candida Albicans ◽

Essential Oils ◽

Antimicrobial Activities ◽

Dilution Method ◽

Klebsiella Pneumonia ◽

Variable Degree ◽

Juniperus Phoenicea ◽

Test Organisms ◽

Western Algeria

Juniperus phoenicea (Family: Cupressaceae) is an evergreen tree widely distributed in North Africa including Algeria. The aim of this investigation was to analyse the antimicrobial potential of essential oils from J. phoenicea on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Streptococcus sp, Bacillus sp and Candida albicans using wells and discs diffusion methods. Broth dilution method was utilized to study the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). The results showed a variable degree of antimicrobial activity. The diameters of inhibition zones for all test organisms were in the ranges of 7–21 mm, while MIC was from 62.5 to >500µl/ml and MBC from 250 to >500µl/ml. The highest antimicrobial activities were observed against Gram positive bacteria followed by Gram negative ones then Candida albicans. The findings provide the evidence that J. phoenicea as a good medicinal plant for further investigations.

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Chemical Composition and Antimicrobial Activity of Teucrium Capitatum L. Subsp. Lusitanicum (Schreb.) T. Navarro & Rosua Essential Oil

Journal of Chromatographic Science ◽

10.1093/chromsci/bmaa086 ◽

2020 ◽

Author(s):

Haïfa Debbabi ◽

Ridha El Mokni ◽

Ibrahim Jlassi ◽

Rajesh K Joshi ◽

Saoussen Hammami

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Antimicrobial Properties ◽

Significant Activity ◽

Bacterial Strains ◽

Gram Positive Bacteria ◽

Sesquiterpene Hydrocarbons ◽

Germacrene D

Abstract Chemical composition and antimicrobial activity of Teucrium capitatum L. subsp. lusitanicum essential oil was investigated for the first time in the present study. Qualitative and quantitative analyses of the chemical composition by gas chromatography and mass spectrometry (GC–FID and GC–MS) revealed the presence of 60 compounds representing 97.6% of the whole constituents. The main compounds were germacrene D (47.1%), spathulenol (5.8%), α-selinene (5.3%), germacrene A (2.9%), δ-cadinene (2.8%) and cubenol (2.7%). In vitro, the antimicrobial activity was investigated against five bacterial strains along with the yeast Candida albicans using broth microdilution assay. T. capitatum subsp. lusitanicum essential oil showed significant activity against the gram-positive bacteria Staphylococcus aureus (MIC = MBC = 78 μg mL−1), Bacillus subtilis (MIC = MBC = 156 μg mL−1) and the yeast C. albicans (MIC = MFC = 156 μg mL−1). The great potential of antimicrobial effects is most likely due to the very high percentage of sesquiterpene hydrocarbons particularly to germacrene D, for which the antimicrobial properties have been previously reported.

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Chemical Composition of Nardostachys grandiflora Rhizome Oil from Nepal – A Contribution to the Chemotaxonomy and Bioactivity of Nardostachys

Natural Product Communications ◽

10.1177/1934578x1501000668 ◽

2015 ◽

Vol 10 (6) ◽

pp. 1934578X1501000 ◽

Cited By ~ 3

Author(s):

Prabodh Satyal ◽

Bhuwan K. Chhetri ◽

Noura S. Dosoky ◽

Ambika Poudel ◽

William N. Setzer

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Cytotoxic Activity ◽

Biological Activities ◽

Mcf 7

The essential oil from the dried rhizome of Nardostachys grandiflora, collected from Jaljale, Nepal, was obtained in 1.4% yield, and a total of 72 compounds were identified constituting 93.8% of the essential oil. The rhizome essential oil of N. grandiflora was mostly composed of calarene (9.4%), valerena-4,7(11)-diene (7.1%), nardol A (6.0%), 1(10)-aristolen-9-ol (11.6%), jatamansone (7.9%), valeranal (5.6%), and cis-valerinic acid (5.7%). The chemical composition of N. grandiflora rhizome oil from Nepal is qualitatively very different than those from Indian, Chinese, and Pakistani Nardostachys essential oils. In this study we have evaluated the chemical composition and biological activities of N. grandiflora from Nepal. Additionally, 1(10)-aristolen-9-ol was isolated and the structure determined by NMR, and represents the first report of this compound from N. grandiflora. N. grandiflora rhizome oil showed in-vitro antimicrobial activity against Bacillus cereus, Escherichia coli, and Candida albicans (MIC = 156 μg/mL), as well as in-vitro cytotoxic activity on MCF-7 cells.

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Chemical Composition and Antibacterial Activity of the Essential Oils of Blainvillea Rhomboidea (Asteraceae)

Natural Product Communications ◽

10.1177/1934578x0600100510 ◽

2006 ◽

Vol 1 (5) ◽

pp. 1934578X0600100

Author(s):

Andreza Maria L. Pires ◽

Maria Rose Jane R. Albuquerque ◽

Edson P. Nunes ◽

Vânia M. M. Melo ◽

Edilberto R. Silveira ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Caryophyllene Oxide ◽

Bacterial Strains ◽

Aerial Parts ◽

Leaf Oil

The essential oils of Blainvellea rhomboidea (Asteraceae) were obtained by hydrodistillation and analyzed by GC/MS and GC/FID. Initially, the essential oil from the aerial parts was investigated. From the 18 identified components, 5-indanol (14.5%) followed by p-cymen-8-ol (10.1%), β-caryophyllene (9.6%), caryophyllene oxide (9.6%), limonene (8.6%), terpinolene (7.8%), and spathulenol (7.7%) were the major constituents. The oil was tested against seven bacterial strains and the results showed significant antimicrobial activity. As a consequence, the essential oils from leaves and from flowers were analyzed separately. The major constituents of the leaf oil were terpinolene (21.2%), β-caryophyllene (19.2%), spathulenol (9.1%), caryophyllene oxide (7.4%), and bicyclogermacrene (7.1%), while the oil of the flowers contained terpinolene (28.1%), 5-indanol (16.3%), p-cymen-8-ol (15.3%) and limonene (14.7%) as prevalent compounds. The oils were tested against the same bacterial strains and the flower oil was the more active. These results indicated that the components of the essential oil from flowers seem to be responsible for the activity.

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