scholarly journals Development of Thermoreversible Dental Gel with Berberine

2020 ◽  
Vol 9 (4) ◽  
pp. 88-92
Author(s):  
A. G. Palvinskiy ◽  
E. O. Bakhrushina ◽  
Z. M. Kozlova ◽  
A. A. Sinitsyna ◽  
I. I. Krasnyuk
Keyword(s):  
Active Substance ◽  
Dosage Form ◽  
Ph Value ◽  
Control Function ◽  
Spectroscopic Method ◽  
Isoquinoline Alkaloid ◽  
Oral Diseases ◽  
Treatment And Prevention ◽  
Optimal Technology ◽  
Biopharmaceutical Properties

Introduction. Dental gel is one of the modern dosage forms with optimal biopharmaceutical properties for the treatment and prevention of oral diseases An isoquinoline alkaloid berberine is the promising active substance with antibacterial and anti-inflammatory effect, capable of forming stable dispersed systems with gel-forming components. It is noteworthy that, despite the pronounced antimicrobial activity of the alkaloid berberine, there is currently no dental dosage form with this component on the pharmaceutical market, and therefore research in this direction is of great interest.Aim. Selection of the optimal technology for obtaining dental gel with berberine for the treatment of oral diseases.Materials and methods. Berberine bisulfate (manufactured by CJSC «Vifitech», Obolensk, Moscow region, RF), poloxamers P407 and P338 (EP, USP/N; BASF, Germany), propylene glycol (USP; BASF, Germany), sodium chloride (Sigma-Aldrich, Germany, Cat. No. S9888), mucin type II from a pig stomach (Sigma-Aldrich, Germany, Cat. No. M2378). In the development of the composition and technology for the preparation of gels, a magnetic stirrer with a temperature control function (C-MAG HS 7 from IKA, Germany) was used. Gels were prepared by the «hot method» and «cold method».Results and discussion. The production of the gel by the «hot method» provided a uniform dosage form. The obtained pH value is within the range of optimal diapason. Measurement of the specific peel force of the model gel sample showed pronounced mucoadhesive properties, which indicates that there is no need to adjust this parameter by introducing additional components into the formulation. The spectroscopic method is applicable for the analysis of berberine gel. It has been found that a significant dilution of the reference substance sample is required to develop an identification procedure.Conclusion. The production of a sample of the dosage form by the «hot method» is optimal, which is probably due to the effect of solubilization and better distribution of the active substance in the base.

RESEARCH ON THE CREATION OF AN APPLICATION SYSTEM FOR THE TREATMENT OF WOUND PROCESSES

2021 ◽  
pp. 74-79
Author(s):  
Petrukhina D.A. ◽  
Pletneva I.V. ◽  
Pokrovskaya Y.S.
Keyword(s):  
Active Substance ◽  
Dosage Form ◽  
Pharmacological Action ◽  
Delivery Systems ◽  
Local Drug Delivery ◽  
Drug Dosing ◽  
Wide Range ◽  
The Matrix ◽  
Optimal Technology ◽  
Medicinal Substances

One of the promising areas of the pharmaceutical industry is the development of local drug delivery systems consisting of a biocompatible matrix and a drug. Such local delivery systems include medicinal films. Ensuring pharmacological action, accuracy of dosing, strong fixation to damaged tissues of the mucous membrane, prolongation of action are the undoubted advantages of medicinal films in comparison with traditional means of treating wound processes. The development of the technology of the drug film containing in its composition the drug cycvalone, which has a wide range of biological activity-antioxidant, anti-inflammatory, antibacterial and others - is an urgent task of pharmacy. As a result of the conducted research, the technology of a drug film with cycvalone in the form of an inclusion complex with β-cyclodextrin based on a matrix of a biodegradable polymer of sodium alginate was developed. It should be noted the availability, safety and pharmacological effectiveness of medicinal substances used in the technology of the dosage form. The inclusion of cyclodextrin in the composition of the film is due to the solubilization of the active substance molecule and an increase in the bioavailability of cycvalone during application therapy. Introduction of cycvalone in the form of complex inclusion also has a positive effect on the uniformity of distribution of the drug in the matrix and, consequently, determines the accuracy of drug dosing. After the release of the active substance, cyclodextrin is able to show detoxifying properties in the wound. The use of a modern biodegradable material as a film-forming agent provides a prolonged action of active substances, high bioavailability and enhanced therapeutic properties of the composition. The values of quality indicators of medicinal films confirming the rational selection of the composition and the optimal technology of the dosage form were experimentally established.

Rebalance the oral microbiota as efficacy tool in endocrine, metabolic, and immune disorders

2020 ◽  
Vol 20 ◽  
Author(s):  
Ciro Gargiulo Isacco ◽  
Andrea Ballini ◽  
Danila De Vito ◽  
Kieu Cao Diem Nguyen ◽  
Stefania Cantore ◽  
...  
Keyword(s):  
Systemic Disease ◽  
Current Treatment ◽  
Oral Bacteria ◽  
The Body ◽  
Oral Microbiota ◽  
Oral Diseases ◽  
Philosophical Approach ◽  
Treatment And Prevention ◽  
Cardio Vascular Disease ◽  
Cardio Vascular

: The current treatment and prevention of oral disorders follow a very sectoral control and procedures considering mouth and its structures as system completely independent from the rest of the body. The main therapeutic approach is carried out on just to keep the levels of oral bacteria and hygiene in an acceptable range compatible with one-way vision of oral-mouth health completely separated from a systemic microbial homeostasis (eubiosis vs dysbiosis). This can negatively impact on the diagnosis of more complex systemic disease and its progression. Dysbiosis is consequence of oral and gut microbiota unbalance with consequences, as reported in current literature, in cardio vascular disease, diabetes mellitus, rheumatoid arthritis, and Alzheimer’s disease. Likewise, there is the need to highlight and develop a novel philosophical approach in the treatments for oral diseases that will necessarily involve non-conventional approaches.

scholarly journals Polymer-Based Carriers in Dental Local Healing—Review and Future Challenges

Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 3948
Author(s):  
Dorota Kida ◽  
Aneta Zakrzewska ◽  
Jacek Zborowski ◽  
Małgorzata Szulc ◽  
Bożena Karolewicz
Keyword(s):  
Active Substance ◽  
Active Pharmaceutical Ingredient ◽  
Drug Carriers ◽  
Topical Administration ◽  
Drug Formulation ◽  
Bone Tissue Regeneration ◽  
Periodontal Pocket ◽  
Oral Diseases ◽  
Future Challenges ◽  
Affected Tissue

Polymers in drug formulation technology and the engineering of biomaterials for the treatment of oral diseases constitute a group of excipients that often possess additional properties in addition to their primary function, i.e., biological activity, sensitivity to stimuli, mucoadhesive properties, improved penetration of the active pharmaceutical ingredient (API) across biological barriers, and effects on wound healing or gingival and bone tissue regeneration. Through the use of multifunctional polymers, it has become possible to design carriers and materials tailored to the specific conditions and site of application, to deliver the active substance directly to the affected tissue, including intra-periodontal pocket delivery, and to release the active substance in a timed manner, allowing for the improvement of the form of application and further development of therapeutic strategies. The scope of this review is polymeric drug carriers and materials developed from selected multifunctional groups of natural, semi-synthetic, and synthetic polymers for topical therapeutic applications. Moreover, the characteristics of the topical application and the needs for the properties of carriers for topical administration of an active substance in the treatment of oral diseases are presented to more understand the difficulties associated with the design of optimal active substance carriers and materials for the treatment of lesions located in the oral cavity.

scholarly journals SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION AMIKACIN IN PURE AND PHARMACEUTICAL DOSAGE FORM

2018 ◽  
Vol 10 (1) ◽  
pp. 38
Author(s):  
Surya Teja G. ◽  
Gurupadayya B. M. ◽  
Venkata Sairam K.
Keyword(s):  
Dosage Form ◽  
Regression Coefficient ◽  
Spectroscopic Method ◽  
Chloranilic Acid ◽  
Pharmaceutical Dosage Form ◽  
Ich Guidelines ◽  
Highly Sensitive ◽  
Acid Reagent ◽  
Chromophoric Group ◽  
Charge Transfer Complexation

Objective: The aim of the study was to develop an easy, sensible and rapid method for the estimation of amikacin in both pure and marketed formulation using the spectrophotometric method.Methods: Due to lack of chromophoric group in the amikacin, it was derivatized with 0.1 mmol chloranillic acid reagent. For the estimation of amikacin, Shimadzu UV-1700 model spectrophotometer with UV probe software was used. The method was based simple on charge transfer complexation of the drug with a p-chloranilic acid reagent to give a purple coloured product which was measured at 524 nm against blank solution.Results: The derivatised product of amikacin was detected at a wavelength of 524 nm. Linearity was observed with the concentration range of 20-100 µg/ml with a regression coefficient of 0.9803. Results of all the parameters were within the acceptance criteria with % RSD less than 2.Conclusion: The spectroscopic method was validated as per ICH guidelines and was found to be applicable for routine quantitative analysis of amikacin in marketed formulations also. The results of linearity, precision, accuracy LOD and LOQ were within the specified limits. The method is highly sensitive, robust, reproducible and specific.

VALIDATED STABILITY INDICATING SPECTROSCOPIC METHOD FOR ESTIMATION OF DEGRADATION BEHAVIOUR OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN TABLET FORMULATION

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (11) ◽  
pp. 53-59
Author(s):  
Tukaram M. Kalyankar ◽  
◽  
Shital S. Dange ◽  
Shivraj B. Hambarde ◽  
Shailesh J. Wadher ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Dosage Form ◽  
Spectroscopic Method ◽  
Stress Conditions ◽  
Spectrometric Method ◽  
Degradation Behaviour ◽  
Stability Indicating ◽  
Photolytic Degradation ◽  
Tablet Dosage

A simple, accurate and precise UV spectrometric method has been developed for the simultaneous determination of valsartan and hydrochlorothiazide in tablet dosage form. Spectra of valsartan and hydrochlorothiazide in methanol and water (50:50 V/V) show λ max at 250.0 nm and 271.4 nm, respectively. Valsartan and hydrochlorothiazide are subjected to various stress conditions like acid, alkali, thermal and photolytic degradation. Beer’s law was obeyed in concentration range of 4- 24 µg mL-1 for valsartan and 0.5-3 µg mL-1 for hydrochlorothiazide at their respective wavelengths. The proposed method was successfully applied to tablet dosage form for determination of both drugs. The percentage recovery of valsartan and hydrochlorothiazide were found to be 100.19 % and 99.51 %, respectively. A novel accurate and precise stability indicating spectroscopic method has been developed for estimation of valsartan and hydrochlorothiazide.

Development and Validation of UV Spectroscopy Method for the Estimation of Dolutegravir in Bulk and Pharmaceutical Dosage Form

2021 ◽  
pp. 188-190
Author(s):  
Vaishnavi Dulange ◽  
G.B. Gajeli
Keyword(s):  
Dosage Form ◽  
Uv Spectroscopy ◽  
Spectroscopic Method ◽  
Water Mixture ◽  
Uv Spectrum ◽  
Pharmaceutical Dosage Form ◽  
Highly Sensitive ◽  
Development And Validation ◽  
The Given ◽  
Regression Correlation

UV spectroscopic method was developed for the estimation of Dolutegravir in bulk and Formulation.The UV spectrum of Dolutegravir in methanol and water mixture showed λ max at 254nm. Beer’s law is valid in the concentration range of 10-50µg/ml. This method was validated for linearity, accuracy, precision, LOD and LOQ. The method has demonstrated excellent linearity over the range of 10-50µg/ml with regression equation y = 0.030x + 0.008 and regression correlation coefficient r2= 0.998. Moreover, the method was found to be highly sensitive with LOD (2.056μg/ml) and LOQ (6.230μg/ml). Depending on results the given method can be successfully applied for assay of Dolutegravir in formulation.

NEW DEVELOPED AND VALIDATED SPECTROSCOPIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TERBINAFINE HYDROCHLORIDE AND FLUCONAZOLE

2020 ◽  
pp. 19-25
Author(s):  
BHUMIKA THAKUR ◽  
INDER KUMAR
Keyword(s):  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Equation ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Pharmaceutical Dosage Form ◽  
Terbinafine Hydrochloride ◽  
Precision Study ◽  
Reproducible Method ◽  
Concentration Levels

Objective: A new, simple, precise, accurate, and reproducible method or simultaneous equation method was developed and validated for the simultaneous estimation of terbinafine hydrochloride (TH) and fluconazole (FLZ) in pure form. Methods: Simultaneous estimation of terbinafine hydrochloride and fluconazole was estimated by the ultraviolet (UV) spectrophotometry method. The method was based on the measurement of absorbance at two wavelengths 222 nm and 239 nm, of terbinafine hydrochloride and fluconazole in 0.1N HCl respectively. Results: Calibration curves terbinafine hydrochloride and fluconazole were found to be linear in the concentration ranges of 0.5-3.0 μg/ml and 80-400 μg/ml, respectively, with their correlation coefficient values (R2) 0.999 and 0.998. LOD and LOQ of TH were found to be 0.067, 0.203 at 222 nm and 0.175, 0.531 at 239 nm, similarly for FLZ; 31.089, 94.210 at 222 nm and 94.380, 286.00 at 239 nm respectively. In the precision study, the % RSD value was found within limits (%). The percentage recovery at various concentration levels varied from 98.50 % to 103.96 % for TH and 97.27 % to 103.83 % for FLZ confirming that the expected method is accurate. Conclusion: It could be concluded from the results obtained in the present study that this method for simultaneous estimation of TH and FLZ in pure is simple, precise, and economical. The proposed method can be applied successfully for the simultaneous estimation of TH and FLZ in the pure and pharmaceutical dosage form.

scholarly journals UV SPECTROPHOTOMETRIC ANALYSIS AND VALIDATION OF ACYCLOVIR IN SOLID DOSAGE FORM

2020 ◽  
pp. 100-103
Author(s):  
AKSHATA LASURE ◽  
AFAQUE ANSARI ◽  
MALLINATH KALSHETTI
Keyword(s):  
Dosage Form ◽  
Quantitative Estimation ◽  
Spectroscopic Method ◽  
Pharmaceutical Formulations ◽  
Spectrophotometric Analysis ◽  
Pharmaceutical Dosage Form ◽  
Solid Dosage ◽  
Ich Guidelines ◽  
Double Beam ◽  
Routine Assay

Objective: A new, economical, sensitive, simple, rapid UV spectrophotometric method has been developed for the estimation of Acyclovir in pure form and pharmaceutical formulation. Methods: This UV method was developed using distilled water as a solvent. In the present method, the wavelength selected for analysis was 254 nm. UV-Visible double beam spectrophotometer (Systronic 2201) was used to carry out the spectral analysis. The ICH guidelines were used to validate the method. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was found in the range of 5-30µg/ml. Accuracy was performed by using a recovery study. The amount of drug recovered was found to be in the range of 100.1-100.5 %. The % RSD value was found to be less than 2. Conclusion: The proposed UV spectroscopic method was found to be accurate, precise, stable, linear, specific, and simple for quantitative estimation of acyclovir in bulk and pharmaceutical dosage form. Hence the present UV spectroscopic method is suitable for the routine assay of acyclovir in bulk and pharmaceutical formulations.

Sign in / Sign up

Export Citation Format

Share Document