scholarly journals Fish oil as effective supplement preventing inflammatory and histopathological alterations in adjuvant-induced arthritis in rats

2021 ◽  
Vol 10 (4) ◽  
pp. e22610414046
Author(s):  
Camila Fernanda Estevão-Silva ◽  
Alessandra Mileni Versuti Ritter ◽  
Franciele Queiroz Ames ◽  
Bruno Ambrósio da Rocha ◽  
Jurandir Fernando Comar ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Fish Oil ◽  
Morphological Changes ◽  
Oral Treatment ◽  
Histological Changes ◽  
Adjuvant Induced Arthritis ◽  
Hematoxylin Staining ◽  
Effect Of Treatment ◽  
Hematoxylin Eosin ◽  
Sirius Red

In this study was investigated the effect of treatment with a fish oil preparation (FOP) on inflammatory and histopathological parameters in a rat model of adjuvant-induced arthritis (AIA). Paw edema, the severity of secondary lesions, the number of synovial leukocytes, and serum nitric oxide and cytokines concentrations were evaluated. Histological changes in cartilage and bone of the femorotibial articulation in the paws were analyzed by hematoxylin/eosin and sirius red-hematoxylin staining. Oral treatment with the FOP (75, 150, and 300 mg/kg) that was initiated on the day of arthritis induction (day zero) and maintained for 21 days inhibited inflammatory signals and prevented morphological changes in cartilage and bone of the femorotibial articulation of the paws. These effects could be at least partially attributable to inhibitory and modulatory actions of the FOP on the production and release of nitric oxide and cytokines that participate in the pathophysiology of AIA.

Postmortem Specificity of Troponin for Acute Miocard Infarction Diagnosis throug Qualitative Dosing from Pericardial Fluid

2018 ◽  
Vol 69 (9) ◽  
pp. 2482-2486
Author(s):  
Iuliana Hunea ◽  
Simona Irina Damian ◽  
Carmen Corina Radu ◽  
Sorin Moldoveanu ◽  
Tatiana Iov
Keyword(s):  
Sudden Cardiac Death ◽  
Cardiac Disease ◽  
Cardiac Death ◽  
Troponin I ◽  
Troponin T ◽  
Morphological Changes ◽  
Pericardial Fluid ◽  
Histological Changes ◽  
Lethal Arrhythmia ◽  
Myocardial Lesions

Cardiac disease is the leading cause of death, and sudden cardiac death occupies the first place in sudden deaths of natural causes. Sudden cardiac death due to lethal arrhythmia may be the first manifestation of a cardiac disease, such cases becoming suspect dead, thus forensic cases. The autopsy performed in such cases may reveal important cardiovascular disease but not obvious macroscopic or histological changes of acute myocardial infarction (IMA), except for cases of survival for several hours after the onset of the symptomatology. Biochemical markers were used to test for myocardial lesions in the absence of morphological changes. Methods for determining myoglobin, CK-MB, troponin T (cTn T), troponin I (cTn I) were introduced to the clinic to diagnose the condition of patients with chest pain as early as the 1990s. The lack of pathognomonic elements in corps investigations, where part of the analysis cannot be carried out, requires verification of the value of the investigations that can be carried out, with reference to the biochemical in the present case, in establishing the diagnosis with certainty.

scholarly journals Anti-Inflammatory Effect of Simonsinol on Lipopolysaccharide Stimulated RAW264.7 Cells through Inactivation of NF-κB Signaling Pathway

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3573
Author(s):  
Lian-Chun Li ◽  
Zheng-Hong Pan ◽  
De-Sheng Ning ◽  
Yu-Xia Fu
Keyword(s):  
Nitric Oxide ◽  
Signaling Pathway ◽  
Morphological Changes ◽  
Raw264.7 Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Factor Α ◽  
Oxide Synthase ◽  
Necrosis Factor

Simonsinol is a natural sesqui-neolignan firstly isolated from the bark of Illicium simonsii. In this study, the anti-inflammatory activity of simonsinol was investigated with a lipopolysaccharide (LPS)-stimulated murine macrophages RAW264.7 cells model. The results demonstrated that simonsinol could antagonize the effect of LPS on morphological changes of RAW264.7 cells, and decrease the production of nitric oxide (NO), tumor necrosis factor α (TNF-α), and interleukin 6 (IL-6) in LPS-stimulated RAW264.7 cells, as determined by Griess assay and enzyme-linked immunosorbent assay (ELISA). Furthermore, simonsinol could downregulate transcription of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 as measured by reverse transcription polymerase chain reaction (RT-PCR), and inhibit phosphorylation of the alpha inhibitor of NF-κB (IκBα) as assayed by Western blot. In conclusion, these data demonstrate that simonsinol could inhibit inflammation response in LPS-stimulated RAW264.7 cells through the inactivation of the nuclear transcription factor kappa-B (NF-κB) signaling pathway.

scholarly journals Electrocution Stigmas in Organ Damage: The Pathological Marks

Diagnostics ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 682
Author(s):  
Gelsomina Mansueto ◽  
Mario Di Napoli ◽  
Pasquale Mascolo ◽  
Anna Carfora ◽  
Pierluca Zangani ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Organ Damage ◽  
Pathological Findings ◽  
Internal Organs ◽  
Histological Changes ◽  
Morphological Findings ◽  
Starting Point ◽  
Autopsy Series ◽  
Golden Standard ◽  
Wide Variability

Background: Diagnostic criteria for electrocution related death are still a challenge in forensic pathology and it seems that the electrical mark is the only reliable evidence. Methods: A comparison of histological and morphological findings of skin and internal organs from an autopsy series of electrocution deaths with those mostly reported in literature as representative for electrocution. Results: The morphological changes of heart, brain and other main internal organs are still unspecific. Organ’s damage observed in electrocution deaths shows a wide variability, not reliable for a certain diagnosis of electrocution. The electrical mark is still the golden standard for diagnosis of electrocution. Conclusions: In electrocution related deaths, pathological findings of the main internal organs are not enough evidence to support with certainty a post-mortem diagnosis that a victim suffered an electrical damage. Although the organ histological changes are undoubtedly the starting point for a better understanding of the fatal even, the diagnosis of death from electrical damage is still a dark and unsolved chapter. The electrical mark still represents a fundamental indicator above all in the medical-legal field, but the identification of pathognomonic elements and signs not limited to the skin alone could be a valid help in the future, especially in unclear cases.

Mercury Kushta Induced Histopathological Changes in Liver of Wister Rats

2021 ◽  
Vol 15 (8) ◽  
pp. 2076-2079
Author(s):  
Sakina Jamil ◽  
Raees Lail ◽  
Zunaira Qayyum ◽  
Zahra Riaz ◽  
Arooj Sattar ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Morphological Changes ◽  
Portal Tract ◽  
Central Vein ◽  
Asian Countries ◽  
Histological Changes ◽  
Liver Histopathology ◽  
Animal Experimental Study ◽  
Hepatocyte Necrosis ◽  
Architecture Development

Background: Heavy metals are the natural constituents of the earth's crust but the indiscriminate human activities have drastically effected their biochemical balance and geochemical cycles. Heavy metals and their compounds have pharmacological importance. These are being used in south Asian countries as component of different medicines. These medicines may have serious side effects on liver. Objectives: To see the histological changes of Kushta which contains mercury, on liver of wister rats. Material and Methods: It was an animal experimental study in which a total of 42 Wistar rats were included and divided into five exposed and one control groups. Morphological changes were observed in liver of rats by using indigenous as well as patent mercury preparations. Results: Morphological changes in liver of exposed rats included hepatocyte swelling, hepatocyte necrosis, hepatocyte apoptosis, disarray of hepatic architecture, development of portal tract inflammation, central vein congestion, sinusoidal congestion and dilatation, development of fatty change and damage to hepatic vascular and liver capsule were seen at the end of 8 weeks. Conclusions: Indigenous herbo-mineral preparation (Kushta) of mercury produces deleterious morphological effects on liver of wister rats. Keywords: Mercury Kushta, Liver, Histopathology

scholarly journals Soybean or Fish Oil Increases Adiponectin and Nitric Oxide Levels and Decreases Blood Pressure in Metabolic Syndrome

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Isaias Dichi ◽  
Andréa Name Colado Simão ◽  
Marcell AB Lovozoy ◽  
Helena Kaminami Morimoto ◽  
Tathiana Name Colado Simão ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Metabolic Syndrome ◽  
Fish Oil

scholarly journals GN1, Glucosamine Analog, attenuate the neuronal hyperactivity of c-fos gene in Adjuvant-Induced Arthritis in Rats

2021 ◽  
Author(s):  
Huma Jawed ◽  
Syed Uzair Ali Shah ◽  
Shahid I. Awan ◽  
Shazia Anjum ◽  
Shabana U. Simjee
Keyword(s):  
Nitric Oxide ◽  
Inflammatory Lesion ◽  
Suppressive Effect ◽  
Histopathological Analysis ◽  
Serum Samples ◽  
Adjuvant Induced Arthritis ◽  
Nitric Oxide Formation ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines

ABSTRACTThe anti-inflammatory and anti-arthritic activity of GN1 (6-hydroxymethyl-3-(1-methylene-allylamino)-tetrahydro-pyran-2,4,5-triol), an analog of glucoseamine was investigated in the adjuvant induced arthritis (AIA) in rats. It was observed that the highly increased hind paw swelling was significantly reduced (p <0.001) with no noticeable retardation of body weight in the GN1 treated arthritic rats as compared to arthritic control rats. The histopathological analysis of isolated rat joints by scaling system classification of different stages appear in arthritic inflammatory lesion development, disclosed the suppressive effect in the inflammation processing in the GN1 treated animals. Different predictive markers such as TNF-α, nitric oxide IL-1β, peroxide and glutathione were also determined in the serum samples of the treated and non-treated arthritic rats in order to measure the intensity of inflammation. Our results show that GN-1 treatment in arthritic groups demonstrated marked reduction in c-fos expression. Results also revealed that GN1 significantly reduces the peroxide production (p < 0.002) and both the pro-inflammatory cytokines TNF-α (p < 0.015) and IL-1β (p < 0.001) in the arthritic rats receiving this treatment. Although, nitric oxide formation was found to be less than the arthritic control, but this reduction was not significant. The level of glutathione was significantly increased in GN1 treated arthritic rats (p < 0.037). These observations suggest that GN1 may help in reducing the inflammation and have anti-arthritic properties.

scholarly journals Amelioration of C - Reactive Protein and Lectin Like Oxidised Low Density Lipoprotein Receptor Complex Induced Endothelial Dysfunction by Oligomeric Proanthocyanidins.

2021 ◽  
Author(s):  
Sankar Jamuna ◽  
Rathinavel Ashokkumar ◽  
Niranjali Devaraj Sivasithamparam
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Endothelial Dysfunction ◽  
Capillary Tube ◽  
Morphological Changes ◽  
Protein Docking ◽  
Pcr Analysis ◽  
Inhibitory Mechanism ◽  
C Reactive Protein ◽  
Reactive Protein

Abstract C-reactive protein (CRP) is a well established biochemical marker for atherosclerosis. Inflammation induced by CRP promotes endothelial dysfunction. Modification of LDL inside the artery wall favors the elevation of this acute phase protein. The mechanism of OxLDL+CRP complex is unrevealed so far. Hence, this mechanism was considered as the important factor to trigger the monocyte to macrophages differentiation which in leads to foam cells formation. Hence this key event should be targeted and focused on how this complex (OxLDL+CRP) proceeds to endothelial dysfunction. OPC is a well known cardioprotective flavon-3-ols. The present study is challenged between the protective roles of OPC against the deleterious effect of this complex (OxLDL+CRP) on endothelial cells. Monolayer of Endothelial cells were incubated with THP-1 monocytes for 48 h supplemented with OxLDL (10mg/ml) + CRP (10 mg/ml) complex and treated with OPC (100mg/ml). Morphological changes, cell migration assay and capillary tube forming assay was carried out. Myeleoperoxidase levels were estimated to determine the adhesion of monocytes onto EC monolayer. RT-PCR analysis of L-Selectin was done. The quantification of NO levels and analysis of mRNA expressions of eNOS is to determine the nitric oxide demand caused due to OxLDL+CRP complex. LOX-1, scavenger receptor levels were analysed by mRNA expression. Proinflammatory markers such as IL-6, MCP-1 and IL-1b were studied. Accumulation of ROS levels were measured fluorimetrically using DCF-DA. Spectrophotometric analysis of Sirius red dye binding collagen levels was observed. Mitochondrial membrane potential was determined by JC-1 dye and cell cycle analysis was done by FACS analysis. Protein –Protein docking was carried out between CRP and LOX-1. This docked protein complex were again docked with OPC and atrovastatin to show the inhibitory mechanism of CRP binding with LOX-1. OPC showed a promising inhibitory mechanism against OxLDL+CRP complex. To emphasis the results OPC treated group showed decreased levels of proinflammatory markers, LOX-1 and L-Selectin levels. Endothelial nitric oxide levels were increased upon OPC treatment and reduction in the ROS levels. Endothelial cells apoptosis was prevented by OPC. Docking studies showed that in the absence of ligands (OPC) binding of CRP and LOX-1 was greater and vice versa in the presence of ligands. To conclude, OxLDL + CRP complex inhibitory effects of OPC could maintain the normal homeostasis.

Upregulation of endothelial nitric oxide synthase in rat aorta after ingestion of fish oil-rich diet

2004 ◽  
Vol 287 (2) ◽  
pp. H567-H572 ◽  
Author(s):  
Diego López ◽  
Xavier Orta ◽  
Kelly Casós ◽  
M. Puy Sáiz ◽  
Pere Puig-Parellada ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Fish Oil ◽  
Endothelial Nitric Oxide Synthase ◽  
Rat Aorta ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

About morphological changes in the kidney in children with infections and some other diseases

1930 ◽  
Vol 26 (2) ◽  
pp. 121-132
Author(s):  
Yu. V. Makarov
Keyword(s):  
Scarlet Fever ◽  
Morphological Changes ◽  
The Other ◽  
Histological Changes ◽  
Other Hand ◽  
Renal Changes ◽  
The One

The issue of pathological and histological changes in the kidneys in children with various infections and other diseases cannot be considered sufficiently researched and worked out. Only in certain infections (scarlet fever) has much attention been paid to the study of the kidneys. Most of the works on the issue of interest to us date back to the time when, on the one hand, insufficient importance was attached to the early dissection of corpses and the freshness of the material, which, as is now known, is of particular importance for the histology of the kidney, on the other hand, such interpretation of the detected changes, which do not correspond to the views and concepts of modern nephropathology; Finally, those changes in views on some diseases that have occurred to date, for example, in the issue of disorders of digestion and nutrition in infants, dictate the need for a different approach to the study of renal changes in these diseases.

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