scholarly journals Evaluation of the cytotoxicity of products obtained from Calotropis procera (Apocynaceae)

2020 ◽  
Vol 9 (12) ◽  
pp. e4391210723
Author(s):  
Ivana Maria Fechine ◽  
Karolayne da Silva Barbosa Alves ◽  
Renam Fellipe da Silveira Muniz ◽  
Caroline Leal Rodrigues Soares ◽  
Harley da Silva Alves ◽  
...  
Keyword(s):  
Thermal Analysis ◽  
Chemical Composition ◽  
Ethanolic Extract ◽  
Leukemic Cells ◽  
Calotropis Procera ◽  
Spectroscopic Techniques ◽  
Cytotoxicity Test ◽  
Good Activity ◽  
Anti Cancer ◽  
Pharmacological Interest

Calotropis procera belongs to the Apocynaceae family and is found in the Northeast of Brazil. Several activities are attributed to this species, such as anti-inflammatory, antibacterial and anti-cancer activities. This research aimed to conduct a bioguided study of C. procera in order to isolate and identify triterpenes of the species and evaluate the cytotoxic activity of products of the plant species. The crude ethanolic extract (CEE) was obtained and partitioned. The hexanic phase was chromatographed giving the compound Cp-1, which was identified using spectroscopic techniques and thermal analysis. It was possible to identify Cp-1 as a triterpene called calotropenyl acetate, already reported in the literature. The cytotoxicity test was performed using the MTT technique and it was observed a good activity of CEE and hexanic phase against HL-60 and KS62 lines with an IC50 ranging between 26.8 ± 0.4 µg mL-1 and 47.0 ± 0.5 µg mL-1, besides no toxicity in normal lines, while Cp-1 showed no relevant activity against the tumor cells tested. The results indicated that the species contains in its chemical composition products with pharmacological interest, in addition to cytotoxic for leukemic cells, which can be further explored in complementary studies.

scholarly journals Phytochemical Evaluation and Anti-Inflammatory Activity of Ethanolic Extract of Calotropis procera Leaves

2020 ◽  
Vol 11 (1) ◽  
pp. 1-6
Author(s):  
Naveed Aslam Dogar ◽  
Hamza Shahid ◽  
Hafiz Usama Shaukat ◽  
M. Abubakar Khan ◽  
Farooq Saleem
Keyword(s):  
Inflammatory Diseases ◽  
Biological Activities ◽  
Diclofenac Sodium ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Calotropis Procera ◽  
Anti Cancer ◽  
Ethanolic Extraction ◽  
Anti Inflammatory

Background: Medicinal plants have been used since centuries to cure various diseases. There is a huge potential to investigate the medicinal impacts of different parts of plants. Roots, stem, leaves and fruits of Calotropis procera are known for their biological activities. Calotropis procera plant shows multiple pharmacological activities like anti-cancer, anti-microbial, antioxidant, anti-malarial, hepatoprotective and anti-diabetic activities. Objectives: The objective of the current research was ethanolic extraction of Calotropis procera leaves and to study the phytochemistry and anti-inflammatory activity. Methodology: In this effort, we used the extract of Calotropis procera leaves for detection of phytochemicals and anti-inflammatory activity in vitro by hypotonicity induced hemolysis on 2% HRBC suspension, using UV-Vis spectrophotometer. Results: Phytochemicals like alkaloids, terpenoids, and flavonoids were present in large amount while tannins, saponins, steroids and cardiac glycosides were in small amount, whereas phlobatannins and anthraquinone were not detected. The potential of the ethanolic extract of Calotropis procera leaves was compared with Diclofenac sodium (100μl/ml, 200μl/ml). The leaves extract of Calotropis procera (100, 200, 300, 400, 500μl/ml each) showed significant anti-inflammatory activity by hypotonicity induced hemolysis on 2% HRBC suspension. Conclusion: The Calotropis procera leaves have potential to cure inflammatory diseases and can be used as anti-inflammatory medicine and analgesic.

Calotropis procera (Aiton) Dryand (Apocynaceae) as an anti-cancer agent against canine mammary tumor and osteosarcoma cells

2021 ◽  
Author(s):  
Ana CarolinaSilveira Rabelo ◽  
Jéssica Borghesi ◽  
Ana Claudia O. Carreira ◽  
Rafael Gonçalves Hayashi ◽  
Fernanda Bessa ◽  
...  
Keyword(s):  
Mammary Tumor ◽  
Calotropis Procera ◽  
Canine Mammary Tumor ◽  
Osteosarcoma Cells ◽  
Anti Cancer ◽  
Cancer Agent

Chemical composition and in vitro activity of Calotropis procera (Ait.) latex on Haemonchus contortus

2016 ◽  
Vol 226 ◽  
pp. 22-25 ◽  
Author(s):  
Géssica S. Cavalcante ◽  
Selene M. de Morais ◽  
Weibson P.P. Andre ◽  
Wesley L.C. Ribeiro ◽  
Ana L.M. Rodrigues ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Haemonchus Contortus ◽  
Vitro Activity ◽  
Calotropis Procera ◽  
In Vitro Activity

scholarly journals Pharmacological Potential and Chemical Composition of Crocus sativus Leaf Extracts

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 10
Author(s):  
Olha Mykhailenko ◽  
Vilma Petrikaite ◽  
Michal Korinek ◽  
Fang-Rong Chang ◽  
Mohamed El-Shazly ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Ethanolic Extract ◽  
Dry Weight ◽  
Crocus Sativus ◽  
Leaf Extracts ◽  
Hplc Fingerprint ◽  
Health Related ◽  
Pharmacological Potential

Crocus sativus L. (saffron) has been traditionally used as a food coloring or flavoring agent, but recent research has shown its potent pharmacological activity to tackle several health-related conditions. Crocus sp. leaves, and petals are the by-products of saffron production and are not usually used in the medicine or food industries. The present study was designed to determine the chemical composition of the water and ethanolic extracts of C. sativus leaves and test their cytotoxic activity against melanoma (IGR39) and triple-negative breast cancer (MDA-MB-231) cell lines by MTT assay. We also determined their anti-allergic, anti-inflammatory, and anti-viral activities. HPLC fingerprint analysis showed the presence of 16 compounds, including hydroxycinnamic acids, xanthones, flavonoids, and isoflavonoids, which could contribute to the extracts’ biological activities. For the first time, compounds such as tectoridin, iristectorigenin B, nigricin, and irigenin were identified in Crocus leaf extracts. The results showed that mangiferin (up to 2 mg/g dry weight) and isoorientin (8.5 mg/g dry weight) were the major active ingredients in the leaf extracts. The ethanolic extract reduced the viability of IGR39 and MDA-MB-231 cancer cells with EC50 = 410 ± 100 and 330 ± 40 µg/mL, respectively. It was more active than the aqueous extract. Kaempferol and quercetin were identified as the most active compounds. Our results showed that Crocus leaves contain secondary metabolites with potent cytotoxic and antioxidant activities.

scholarly journals Study on antibacterial activity of Calotropis procera

2014 ◽  
Author(s):  
Muhammad Muzammal
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Salmonella Typhi ◽  
Calotropis Procera ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Crude Extracts ◽  
Mic Value

Comparative study of plant extracts crude and aqueous, methanolic and ethanolic with antibiotics, provide evidence that calotropis procera extracts has the similar antibacterial activity as these antibiotics against test pathogens i.e. Salmonella typhi and E.coli. The analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and flower of Calotropis procera was carried out in disc method and also determined MIC value at 600nm through optical density using spectrophotometer. The zone of inhibition produced by extracts was examined and compares it with zone produced by antibiotics. The effect exhibited by ethanolic extract of leaves and flower was significantly greater than the aqueous and methanolic extract of leaves and flower. Crude extracts i.e. latex, leaves, fruit and flower crude extracts. Among them, flower crude extracts shows similar zone of inhibition to test anitibiotics.while in MIC value, we made different concentration of extracts and antibiotics.i.e for crude we made 25%, 50%.75% and 100% concentration of crude juice and for the aqueous, methanolic and ethanolic we made 0.5mg/mL,1mg/mL,2mg/mL,3mg/mL,4mg/mL, 5mg/mL and 6mg/mL in Dimethyl salfoxide, and same antibiotics concentration. Aqueous leaves extracts show MIC at 0.5mg/ml against E.coli while against Salmonella it shows MIC at 1mg/ml... We also determine phytochemical analysis for presences of different compounds in crude extracts. The obtained results provide a support for the use of Calotropis procera, in traditional medicine and suggest its further advance investigation.

scholarly journals A New 3-Prenyl-2-flavene and Other Extractives from Baphia massaiensis and Their Antimicrobial Activities

2018 ◽  
Vol 13 (4) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Ngonye Keroletswe ◽  
Runner R. T. Majinda ◽  
Ishmael B. Masesane
Keyword(s):  
Escherichia Coli ◽  
2D Nmr ◽  
Antimicrobial Activities ◽  
Spectroscopic Techniques ◽  
Good Activity ◽  
Gram Negative ◽  
E Coli ◽  
Chromatographic Techniques ◽  
Inhibition Zones ◽  
First Time

One new 3-prenyl-2-flavene, named baphiflavene A, 1, and eleven known compounds, 2-12, were isolated and reported for the first time from Baphia massaiensis using several chromatographic techniques. Their structures were elucidated using different spectroscopic techniques; 1D and 2D-NMR, HRMS, GC-MS, UV/Vis, FTIR and by comparison with literature data. The isolates were tested against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli and Candida albicans to establish their preliminary antimicrobial activities. The results revealed that compound 1 had moderate activities against both Gram positive ( B. subtilis and S. aureus) and Gram negative ( E. coli and P. aeruginosa) bacteria, and good activity against C. albicans with inhibition zones of 10–23 mm (compared to 19 mm for chloramphenicol and miconazole standards). To the best of our knowledge, this is the first phytochemical work reported on Baphia massaiensis.

scholarly journals Anti-cancer capacity of plasma-treated PBS: effect of chemical composition on cancer cell cytotoxicity

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Wilma Van Boxem ◽  
Jonas Van der Paal ◽  
Yury Gorbanev ◽  
Steven Vanuytsel ◽  
Evelien Smits ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Cancer Cell ◽  
Cell Cytotoxicity ◽  
Anti Cancer
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