Disinfectant properties of slaked lime with sodium chloride

2020 ◽  
pp. 15-17
Author(s):  
Magomedzapir S. Saypullaev ◽  
◽  
Artigat M. Batyrova ◽  
Tamila B. Mirzoeva ◽  
Ali U. Koychuev ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sodium Chloride ◽  
Rough Surfaces ◽  
Wide Spectrum ◽  
Hydrated Lime ◽  
Coli Strain ◽  
Aureus Strain ◽  
Foaming Agent ◽  
Slaked Lime ◽  
Lime Solution

The search of new disinfectants with a wide spectrum of antimicrobial activity of low toxicity and not hazardous in use, not requiring of creation of special storage conditions, convenient in use and not adversely affecting on processed items, affordable, is an urgent task of veterinary science. The article presents the results of laboratory tests of new bactericidal compositions, which include: 20% hydrated lime, 1.3 and 5% sodium chloride, as well as foaming agent PO-6K on test surfaces made of stainless steel, galvanized gland, tile, wood and concrete. As a test of microorganisms, museum cultures of E. coli (strain 1257) and Staphylococcus aureus (strain 209-P) were used. During the experiments, disinfection regimes were established (concentration, exposure, consumption of disinfectant) for disinfecting of smooth and rough test surfaces. At the same time, it was found that slaked lime solutions of 10 and 15% concentration did not have disinfecting activity even after triple whitening or irrigation of test surfaces contaminated with E. ooli (strain 1257), St. aureus (strain 209-P) at a rate of 0,5 l/m2 for smooth and 1 l/m2 - for rough surfaces. 20% solution of slaked lime, which destroys only Escherichia coli on smooth surfaces in 3 hours of exposure at the rate of 0,5 l/m2, and rough surfaces in 24 hours of exposure at the rate of 1,0 l/m2, has a disinfecting effect. At the same time, adding 1,3 and 5% sodium chloride to the slaked lime solution, the disinfecting activity of slaked lime also increases sharply; adding to 20% slaked lime 3 and 5% sodium chloride disinfects Escherichia coli and staphylococcus in 3 hours, based on smooth 0,5 l/m2, and for rough surfaces - 1 l/m2. Studies have also found that the addition of 3-5% sodium chloride, 5% foaming agent to 20% slaked lime solution reduces the consumption of the disinfectant in 2 times without reflecting the indicators of disinfection efficiency. When a foaming agent is added to the solution, the resistance (adhesion) of the foam on the vertical and ceiling surfaces increases on 14-18 minutes, and on the floor the foam is held in 1-1,5 hours, which helps to reduce the runoff of the solution.

DISINFECTION EFFECTIVENESS OF SLAKED LIME WITH NATRIUM CHLORIDE

1970 ◽  
pp. 58-61
Author(s):  
M. S. Saypullaev ◽  
A. M. Batyrova
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Sodium Chloride ◽  
Chloride Solution ◽  
Rough Surfaces ◽  
Hydrated Lime ◽  
Smooth Surfaces ◽  
Slaked Lime ◽  
Lime Solution ◽  
And Staphylococcus Aureus

Veterinary practice constantly needs highly effective, environmentally friendly, economically viable, non-specific means of protecting of animals from infectious diseases. It is known that various disinfectants are used for disinfection by irrigation, including: 5% hot solution of soda ash, 3% hot emulsion of disinfecting creolin, 1-2% formaldehyde solution, 2% sodium hydroxide solution, 5% chloramine solution, 1% glutaric solution aldehyde and others, calculation 0.5-1.0 l/m2 of the surface of the objects of veterinary surveillance. The disadvantage of these disinfectants is that many of them are toxic for humans and animals, have very irritating actirn on the respiratory tract, as well as the mucous membranes of the eyes. Also is known a disinfectant calcium hydroxide (slaked lime) of 10-20% concentration for the disinfection of objects of veterinary surveillance. This tool in comparison with the above mentioned is not particularly toxic and environmentally unsafe for the environment. The disadvantage of slaked lime is the low bactericidal activity against infectious diseases of the 1,2,3 category of resistance. It was found that 10% and 15% solutions do not disinfect Escherichia coli (pcs. 1257) and Staphylococcus aureus (pcs. 209 r), and 20% solution of hydrated lime disinfects Escherichia coli only after cross-application in 3 hours on smooth surfaces (stainless steel, galvanized iron, tile) and after 24 hours on rough surfaces (wood, concrete), at the rate of 1.0 l/m2. To increase the bactericidal and disinfection activity, 1.3 and 5% sodium chloride solution was added to a solution of 20% slaked lime. In this case, calcium chloride (CaCl2) and sodium hydroxide (NaOH) are formed, which can increase the disinfection activity by increasing the ionic strength of slaked lime solution. As experiments showed, the addition of 1% solution of sodium chloride in 20% solution of hydrated lime contributed to the disinfection of Escherichia coli and Staphylococcus aureus for 3 hours of exposure at rate of 0.5 l/m2, and rough -for 3 hours, at a rate of 1.0 l/m2. When 3% sodium chloride solution was added to 20% slaked lime solution, disinfection of smooth surfaces from Escherichia coli occurred in 1 hour, on rough surfaces in 3 hours at the rate of 0.5 and 1.0 l/m2, respectively. Staphylococcus aureus was disinfected on smooth surfaces in 3 hours at a rate of 0.5 l/m2, and on rough surfaces in 3 hours at a rate of 1.0 l/m2.

MEANS OF «CHLORTAB» – FOR DISINFECTION OF OBJECTS OF VETNADZOR

2018 ◽  
Vol 1 (2) ◽  
pp. 47-51
Author(s):  
N. I. Popov ◽  
◽  
M. S. Saipullayev ◽  
A. U. Koychuev ◽  
◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Stainless Steel ◽  
Laboratory Tests ◽  
Rough Surfaces ◽  
Coli Strain ◽  
Aureus Strain ◽  
E Coli

The results of laboratory tests carried out on test surfaces made of stainless steel, tile and metlakh tile, wood, concrete are presented. As test microorganisms, museum cultures of Escherichia coli (strain 1257), Staphylococcus aureus (strain 209R), Mycobacteria (strain B-5), B. сereus (pcs. 96) were used. In the course of the experiments, disinfection regimes (concentration, exposure, consumption of disinfectant) were established for disinfecting smooth and rough surfaces. In this case, effective disinfection of all types of test surfaces contaminated with E. coli and S. aureus was obtained by processing 0,7% solution, respectively, for 1 and 3 hours at a rate of 0,5 liters / m2. Disinfection of test surfaces contaminated with Mycobacteria (strain B-5) and B. cereus (pcs. 96) spores reached 4,0%, respectively, after a single and 5,0% concentration after double treatment at a rate of 0,5 l/m2 for 24 hours exposure.

scholarly journals Physicochemical properties of the new drug Penox-1

2021 ◽  
Author(s):  
A.M. Batyrova ◽  
M.S. Saypullaev
Keyword(s):  
Surface Tension ◽  
Sodium Chloride ◽  
Physicochemical Properties ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Foam Stability ◽  
Chloride Content ◽  
Hydrated Lime ◽  
Foam Layer ◽  
Slaked Lime

The physicochemical properties of the substances included in the composition of the new disinfectant Penox-1 have been studied. Assessment of its disinfecting properties of veterinary in-spection objects, with the study of solubility in water and sodium chloride, surface tension of solutions, stability and adhesion of foam on vertical and ceiling test surfaces in laboratory conditions. At the initial stage, the temperature dependence of the solubility of slaked lime in water was es-tablished. It was determined by the change in the activity of OH- ions as the temperature of the over-saturated solution of slaked lime increased. Distilled water was used as a solvent. The solubility of slaked lime was also studied by adding 1.0; 3.0; 5.0 and 10.0 % sodium chloride solution. The experi-ments were carried out according to the method "On the procedure for testing new disinfectants for veterinary practice (1987)". Assessment with the study of the surface tension of solutions, resistance and adhesion of foam was carried out at the Institute of Physics, DSCRAN, also according to the indi-cated method. As a result of the experiments, it was found that with an increase in water temperature, the sol-ubility of slaked lime in it decreases. Adding 1 and 3% sodium chloride solution to a 90 % solution of slaked lime, the solubility increases by 2.15 and 2.2 times, respectively. With an increase in sodium chloride content to 5%, the solubility of hydrated lime begins to decrease. The presence in the solution of 20 % slaked lime and 3 % sodium chloride solution, 5 % foaming agent (PO-6k) reduces surface ten-sion and increases foam stability. It was also found that the thickness of the foam layer depends on the type of surface. The critical thickness of the foam layer on vertical surfaces was from 1 to 4 cm, on the ceiling – from 2 to 7 cm. The stability of the foam depends on its concentration in the solution. The foam content up to 5–6 % in the solution increased the resistance on vertical surfaces up to 16 minutes, and on ceiling surfaces up to 18 minutes.

UJI ANTIBAKTERI TEPUNG UMBI PORANG (Amorphophallus konjac) TERHADAP Staphylococcus aureus dan Escherichia coli DENGAN METODE DIFUSI DISK

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Sulkarnain Sulkarnain ◽  
Laily Agustina ◽  
Jamilah Jamilah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Coli Strain ◽  
Blood Agar ◽  
Aureus Strain ◽  
Strain Atcc ◽  
E Coli ◽  
Amorphophallus Konjac

Umbi porang (Amorphophallus konjac) termasuk salah satu jenis tanaman talas-talasan dan merupakan tanaman lokal Indonesia yang banyak tumbuh di hutan. Umbi porang memiliki kandungan glukomanan yang cukup tinggi, yaitu sekitar 55% dari berat kering umbi. Umbi porang juga memiliki kandungan berupa protein yang memiliki peran penting dalam mencegah pertumbuhan mikroba. Jenis protein yang dapat berfungsi sebagai antimikroba adalah lektin. Penelitian ini bertujuan untuk mengetahui efektifitas antibakteri dari tepung umbi porang dalam menghambat Staphylococcus aureus dan Escherichia coli yang dapat menyebabkan penyakit pada unggas. Bahan yang digunakan dalam penelitian ini yaitu tepung umbi porang, isolat kuman, Staphylococcus aureus strain ATCC 25923, Escherichia coli strain ATCC 25922, media muller hinton agar (MHA), blood agar dan mac conkey agar, antibiotik disk, kertas disk kosong, alkohol 70% dan aquades. Data yang diperoleh dianalisis dengan RAL (Rancangan Acak Lengkap) yang terdiri atas empat perlakuan dan empat ulangan. Hasil penelitan menujukkan bahwa luas zona hambat berpengaruh nyata (P<0,05) baik pada bakteri S.aureus maupun E. coli.. Dari hasil tersebut dapat disimpulkan bahwa pemberian tepung umbi porang hingga level 4,5% mampu menghambat pertumbuhan S.aureus dan E. coli.. Daya hambat tertinggi terdapat pada bakteri Gram positif dan daya hambat terendah terdapat pada bakteri Gram negatif. Kata Kunci: Antibakteri, Tepung Umbi Porang, S.aureus, E. coli.

Antibacterial Activity and Time-kill Kinetics of Positive Enantiomer of α-pinene Against Strains of Staphylococcus aureus and Escherichia coli

2018 ◽  
Vol 18 (11) ◽  
pp. 917-924 ◽  
Author(s):  
Leticia de Sousa Eduardo ◽  
Ticiane Costa Farias ◽  
Siluana Benvindo Ferreira ◽  
Paula Benvindo Ferreira ◽  
Zilka Nanes Lima ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Coli Strain ◽  
Aureus Strain ◽  
Bacterial Killing ◽  
Antibiotic Resistant ◽  
Death Kinetics ◽  
Time Kill

Research on new antimicrobial agents is needed, as more and more microorganisms that cause antibiotic-resistant diseases are emerging commercially. In this group, we can find strains of Staphylococcus aureus and Escherichia coli, which are highly opportunistic species. Faced with this perspective, research using essential oils present in plants is emerging as a therapeutic alternative for the treatment of antimicrobial infections. Many of these oils have, in their composition, monoterpene α-pinene, that shows to have antibacterial activity. The purpose of this research is to evaluate the antimicrobial activity of the positive enantiomer of α-pinene against strains of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The methodologies used were: Disc-diffusion test, broth microdilution and bacterial death kinetics, all of which were standardized by CLSI. As a result, inhibition halos of 11 mm was obtained for the gram-positive strain and 12 mm for the gram-negative strain, both at the same concentration, 160 µl / ml. In addition, it was possible to observe with the death curve that the concentrations (1.25 µl/mL and 2.5 µl/mL of the (+)-α-pinene were able to eliminate the formation of bacterial colonies at one time of exposure of 2 hours for the E. coli strain. However, the death curve of the S. aureus strain was characterized by non-elimination of bacterial colonies at a 24 hours exposure time used for the experiment. Only amikacin evidenced its bacterial killing rate of all colonies within two hours of exposure. At the end, it was possible to verify the activity of the phytoconstituent against Escherichia coli strains ATCC 25922 and Staphylococcus aureus ATCC 25923, recommending the continuity of the studies with the use of different methodologies so that (+) - α-pinene in the future can be a compound used in antimicrobial therapy.

scholarly journals Regulation of Expression of the TIR-Containing Protein C Gene of the Uropathogenic Escherichia coli Strain CFT073

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 549
Author(s):  
Julia Ittensohn ◽  
Jacqueline Hemberger ◽  
Hannah Griffiths ◽  
Maren Keller ◽  
Simone Albrecht ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein C ◽  
Interleukin 1 ◽  
Coli Strain ◽  
Uropathogenic Escherichia Coli ◽  
Reporter Construct ◽  
Regulation Of Expression ◽  
E Coli ◽  
Strain Cft073 ◽  
Myeloid Differentiation Factor

The uropathogenic Escherichia coli strain CFT073 causes kidney abscesses in mice Toll/interleukin-1 receptor domain-containing protein C (TcpC) dependently and the corresponding gene is present in around 40% of E. coli isolates of pyelonephritis patients. It impairs the Toll-like receptor (TLR) signaling chain and the NACHT leucin-rich repeat PYD protein 3 inflammasome (NLRP3) by binding to TLR4 and myeloid differentiation factor 88 as well as to NLRP3 and caspase-1, respectively. Overexpression of the tcpC gene stopped replication of CFT073. Overexpression of several tcpC-truncation constructs revealed a transmembrane region, while its TIR domain induced filamentous bacteria. Based on these observations, we hypothesized that tcpC expression is presumably tightly controlled. We tested two putative promoters designated P1 and P2 located at 5′ of the gene c2397 and 5′ of the tcpC gene (c2398), respectively, which may form an operon. High pH and increasing glucose concentrations stimulated a P2 reporter construct that was considerably stronger than a P1 reporter construct, while increasing FeSO4 concentrations suppressed their activity. Human urine activated P2, demonstrating that tcpC might be induced in the urinary tract of infected patients. We conclude that P2, consisting of a 240 bp region 5′ of the tcpC gene, represents the major regulator of tcpC expression.

Sign in / Sign up

Export Citation Format

Share Document