scholarly journals Enhancing Bioactivity of Hydroxyapatite Scaffolds Using Fibrous Type I Collagen

2021 ◽  
Vol 9 ◽  
Author(s):  
Paola Nitti ◽  
Sanosh Kunjalukkal Padmanabhan ◽  
Serena Cortazzi ◽  
Eleonora Stanca ◽  
Luisa Siculella ◽  
...  
Keyword(s):  
Weight Loss ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Composite Structures ◽  
Type I Collagen ◽  
Mechanical Stability ◽  
Cold Water ◽  
Essential Feature ◽  
Bone Tissue Regeneration ◽  
Type I

In the field of bone tissue regeneration, the development of osteoconductive and osteoinductive scaffolds is an open challenge. The purpose of this work was the design and characterization of composite structures made of hydroxyapatite scaffold impregnated with a collagen slurry in order to mimic the bone tissue structure. The effect of magnesium and silicon ions enhancing both mechanical and biological properties of partially substituted hydroxyapatite were evaluated and compared with that of pure hydroxyapatite. The use of an innovative freeze-drying approach was developed, in which composite scaffolds were immersed in cold water, frozen and then lyophilized, thereby creating an open-pore structure, an essential feature for tissue regeneration. The mechanical stability of bone scaffolds is very important in the first weeks of slow bone regeneration process. Therefore, the biodegradation behavior of 3D scaffolds was evaluated by incubating them for different periods of time in Tris-HCl buffer. The microstructure observation, the weight loss measurements and mechanical stability up to 28 days of incubation (particularly for HA-Mg_Coll scaffolds), revealed moderate weight loss and mechanical performances reduction due to collagen dissolution. At the same time, the presence of collagen helps to protect the ceramic structure until it degrades. These results, combined with MTT tests, confirm that HA-Mg_Coll scaffolds may be the suitable candidate for bone remodeling.

scholarly journals Surface-Modified Poly(l-lactide-co-glycolide) Scaffolds for the Treatment of Osteochondral Critical Size Defects—In Vivo Studies on Rabbits

2020 ◽  
Vol 21 (20) ◽  
pp. 7541
Author(s):  
Małgorzata Krok-Borkowicz ◽  
Katarzyna Reczyńska ◽  
Łucja Rumian ◽  
Elżbieta Menaszek ◽  
Maciej Orzelski ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Photoelectron Spectroscopy ◽  
Sessile Drop ◽  
Bone Tissue Regeneration ◽  
In Vivo Studies ◽  
In Vivo Model ◽  
Type I ◽  
X Ray

Poly(l-lactide-co-glycolide) (PLGA) porous scaffolds were modified with collagen type I (PLGA/coll) or hydroxyapatite (PLGA/HAp) and implanted in rabbits osteochondral defects to check their biocompatibility and bone tissue regeneration potential. The scaffolds were fabricated using solvent casting/particulate leaching method. Their total porosity was 85% and the pore size was in the range of 250–320 µm. The physico-chemical properties of the scaffolds were evaluated using scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), sessile drop, and compression tests. Three types of the scaffolds (unmodified PLGA, PLGA/coll, and PLGA/HAp) were implanted into the defects created in New Zealand rabbit femoral trochlears; empty defect acted as control. Samples were extracted after 1, 4, 12, and 26 weeks from the implantation, evaluated using micro-computed tomography (µCT), and stained by Masson–Goldner and hematoxylin-eosin. The results showed that the proposed method is suitable for fabrication of highly porous PLGA scaffolds. Effective deposition of both coll and HAp was confirmed on all surfaces of the pores through the entire scaffold volume. In the in vivo model, PLGA and PLGA/HAp scaffolds enhanced tissue ingrowth as shown by histological and morphometric analyses. Bone formation was the highest for PLGA/HAp scaffolds as evidenced by µCT. Neo-tissue formation in the defect site was well correlated with degradation kinetics of the scaffold material. Interestingly, around PLGA/coll extensive inflammation and inhibited tissue healing were detected, presumably due to immunological response of the host towards collagen of bovine origin. To summarize, PLGA scaffolds modified with HAp are the most promising materials for bone tissue regeneration.

scholarly journals Biofabrication of SDF-1 Functionalized 3D-Printed Cell-Free Scaffolds for Bone Tissue Regeneration

2020 ◽  
Vol 21 (6) ◽  
pp. 2175 ◽  
Author(s):  
Alina Lauer ◽  
Philipp Wolf ◽  
Dorothea Mehler ◽  
Hermann Götz ◽  
Mehmet Rüzgar ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Growth ◽  
Collagen Type ◽  
Biomechanical Testing ◽  
Collagen Type I ◽  
Bone Tissue Regeneration ◽  
Control Group ◽  
Type I

Large segmental bone defects occurring after trauma, bone tumors, infections or revision surgeries are a challenge for surgeons. The aim of our study was to develop a new biomaterial utilizing simple and cheap 3D-printing techniques. A porous polylactide (PLA) cylinder was printed and functionalized with stromal-derived factor 1 (SDF-1) or bone morphogenetic protein 7 (BMP-7) immobilized in collagen type I. Biomechanical testing proved biomechanical stability and the scaffolds were implanted into a 6 mm critical size defect in rat femur. Bone growth was observed via x-ray and after 8 weeks, bone regeneration was analyzed with µCT and histological staining methods. Development of non-unions was detected in the control group with no implant. Implantation of PLA cylinder alone resulted in a slight but not significant osteoconductive effect, which was more pronounced in the group where the PLA cylinder was loaded with collagen type I. Addition of SDF-1 resulted in an osteoinductive effect, with stronger new bone formation. BMP-7 treatment showed the most distinct effect on bone regeneration. However, histological analyses revealed that newly formed bone in the BMP-7 group displayed a holey structure. Our results confirm the osteoinductive character of this 3D-biofabricated cell-free new biomaterial and raise new options for its application in bone tissue regeneration.

scholarly journals Development and Biocompatibility of Collagen-Based Composites Enriched with Nanoparticles of Strontium Containing Mesoporous Glass

Materials ◽  
2019 ◽  
Vol 12 (22) ◽  
pp. 3719 ◽  
Author(s):  
Giorgia Montalbano ◽  
Giorgia Borciani ◽  
Carlotta Pontremoli ◽  
Gabriela Ciapetti ◽  
Monica Mattioli-Belmonte ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Type I Collagen ◽  
Bone Tissue Regeneration ◽  
In Vitro Tests ◽  
Type I ◽  
Natural Polymers ◽  
Bioactive Glasses ◽  
Composition And Structure

In the last years bone tissue engineering has been increasingly indicated as a valid solution to meet the challenging requirements for a healthy bone regeneration in case of bone loss or fracture. In such a context, bioactive glasses have already proved their great potential in promoting the regeneration of new bone tissue due to their high bioactivity. In addition, their composition and structure enable us to incorporate and subsequently release therapeutic ions such as strontium, enhancing the osteogenic properties of the material. The incorporation of these inorganic systems in polymeric matrices enables the formulation of composite systems suitable for the design of bone scaffolds or delivery platforms. Among the natural polymers, type I collagen represents the main organic phase of bone and thus is a good candidate to develop biomimetic bioactive systems for bone tissue regeneration. However, alongside the specific composition and structure, the key factor in the design of new biosystems is creating a suitable interaction with cells and the host tissue. In this scenario, the presented study aimed at combining nano-sized mesoporous bioactive glasses produced by means of a sol–gel route with type I collagen in order to develop a bioactive hybrid formulation suitable for bone tissue engineering applications. The designed system has been fully characterized in terms of physico-chemical and morphological analyses and the ability to release Sr2+ ions has been studied observing a more sustained profile in presence of the collagenous matrix. With the aim to improve the mechanical and thermal stability of the resulting hybrid system, a chemical crosslinking approach using 4-star poly (ethylene glycol) ether tetrasuccinimidyl glutarate (4-StarPEG) has been explored. The biocompatibility of both non-crosslinked and 4-StarPEG crosslinked systems was evaluated by in vitro tests with human osteoblast-like MG-63 cells. Collected results confirmed the high biocompatibility of composites, showing a good viability and adhesion of cells when cultured onto the biomaterial samples.

scholarly journals Collagen Hybrid Formulations for the 3D Printing of Nanostructured Bone Scaffolds: An Optimized Genipin-Crosslinking Strategy

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1681
Author(s):  
Giorgia Montalbano ◽  
Giorgia Borciani ◽  
Giorgia Cerqueni ◽  
Caterina Licini ◽  
Federica Banche-Niclot ◽  
...  
Keyword(s):  
3D Printing ◽  
Bone Tissue ◽  
Type I Collagen ◽  
Bone Tissue Regeneration ◽  
Type I ◽  
Bioactive Glasses ◽  
Bioactive Materials ◽  
Complex Optimization ◽  
Strontium Ions ◽  
Premature Release

Bone-tissue regeneration induced by biomimetic bioactive materials is the most promising approach alternative to the clinical ones used to treat bone loss caused by trauma or diseases such as osteoporosis. The goal is to design nanostructured bioactive constructs able to reproduce the physiological environment: By mimicking the natural features of bone tissue, the cell behavior during the regeneration process may be addressed. At present, 3D-printing technologies are the only techniques able to design complex structures avoiding constraints of final shape and porosity. However, this type of biofabrication requires complex optimization of biomaterial formulations in terms of specific rheological and mechanical properties while preserving high biocompatibility. In this work, we combined nano-sized mesoporous bioactive glasses enriched with strontium ions with type I collagen, to formulate a bioactive ink for 3D-printing technologies. Moreover, to avoid the premature release of strontium ions within the crosslinking medium and to significantly increase the material mechanical and thermal stability, we applied an optimized chemical treatment using ethanol-dissolved genipin solutions. The high biocompatibility of the hybrid system was confirmed by using MG-63 and Saos-2 osteoblast-like cell lines, further highlighting the great potential of the innovative nanocomposite for the design of bone-like scaffolds.

scholarly journals Biologically Inspired Collagen/Apatite Composite Biomaterials for Potential Use in Bone Tissue Regeneration—A Review

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1748 ◽  
Author(s):  
Barbara Kołodziejska ◽  
Agnieszka Kaflak ◽  
Joanna Kolmas
Keyword(s):  
Bone Tissue ◽  
Orthopedic Surgery ◽  
Type I Collagen ◽  
Bone Tissue Regeneration ◽  
Type I ◽  
Biologically Inspired ◽  
Natural Composite ◽  
Potential Use

Type I collagen and nanocrystalline-substituted hydroxyapatite are the major components of a natural composite—bone tissue. Both of these materials also play a significant role in orthopedic surgery and implantology; however, their separate uses are limited; apatite is quite fragile, while collagen’s mechanical strength is very poor. Therefore, in biomaterial engineering, a combination of collagen and hydroxyapatite is used, which provides good mechanical properties with high biocompatibility and osteoinduction. In addition, the porous structure of the composites enables their use not only as bone defect fillers, but also as a drug release system providing controlled release of drugs directly to the bone. This feature makes biomimetic collagen–apatite composites a subject of research in many scientific centers. The review focuses on summarizing studies on biological activity, tested in vitro and in vivo.

Porous Collagen-Hydroxyapatite Scaffolds With Mesenchymal Stem Cells for Bone Regeneration

2015 ◽  
Vol 41 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Li Ning ◽  
Hans Malmström ◽  
Yan-Fang Ren
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue ◽  
Type I Collagen ◽  
Human Mesenchymal Stem Cells ◽  
Bone Tissue Regeneration ◽  
Type I ◽  
Periodontal Ligament Stem Cells ◽  
Tissue Engineering Construct ◽  
Bone Grafting Materials

Current bone grafting materials have significant limitations for repairing maxillofacial and dentoalveolar bone deficiencies. An ideal bone tissue-engineering construct is still lacking. The purpose of the present study was first to synthesize and develop a collagen-hydroxyapatite (Col-HA) composite through controlled in situ mineralization on type I collagen fibrils with nanometer-sized apatite crystals, and then evaluate their biologic properties by culturing with mouse and human mesenchymal stem cells (MSCs). We synthesized Col-HA scaffolds with different Col:HA ratios. Mouse C3H10T1/2 MSCs and human periodontal ligament stem cells (hPDSCs) were cultured with scaffolds for cell proliferation and biocompatibility assays. We found that the porous Col-HA composites have good biocompatibility and biomimetic properties. The Col-HA composites with ratios 80:20 and 50:50 composites supported the attachments and proliferations of mouse MSCs and hPDSCs. These findings indicate that Col-HA composite complexes have strong potentials for bone tissue regeneration.

scholarly journals Study on proanthocyanidins crosslinked collagen membrane for guided bone tissue regeneration

2021 ◽  
Vol 19 ◽  
pp. 228080002110053
Author(s):  
Hongfa Yang ◽  
Wai-Ching Liu ◽  
Xinrui Liu ◽  
Yunqian Li ◽  
Chingpo Lin ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Pore Size ◽  
Cell Line ◽  
Bone Tissue ◽  
Phosphatase Activity ◽  
Tissue Regeneration ◽  
Alkaline Phosphatase Activity ◽  
Bone Tissue Regeneration ◽  
Type I ◽  
Barrier Membrane

The goal of this study is to understand the ability of a newly developed barrier membrane to enhance bone tissue regeneration. Here in this study we present the in vitro characterization of the barrier membrane made from type I collagen and crosslinked by oligomeric proanthocyanidins (OPCs). The effects of the membrane (P-C film) on cell cycle, proliferation, alkaline phosphatase activity, and mineralization were evaluated using the human osteoblast cell line MG-63, while the barrier ability was examined using MG-63 cells, as well as the human skin fibroblast cell line WS-1. The pore size is one of the factors that plays a key role in tissue regeneration, therefore, we evaluated the pore size of the membrane using a capillary flow porometer. Our results showed that the mean pore size of the P-C film was approximately 7–9 µm, the size known to inhibit cell migration across the membrane. The P-C film also demonstrated excellent cell viability and good biocompatibility, since the cell number increased with time, with MG-63 cells proliferating faster on the P-C film than in the cell culture flask. Furthermore, the P-C film promoted osteoblast differentiation, resulting in higher alkaline phosphatase activity and mineralization. Therefore, our results suggest that this P-C film has a great potential to be used in guided bone regeneration during periodontal regeneration and bone tissue engineering.

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