scholarly journals Advanced DNA Detection via Multispectral Plasmonic Metasurfaces

2021 ◽  
Vol 9 ◽  
Author(s):  
Valentina Di Meo ◽  
Massimo Moccia ◽  
Gennaro Sanità ◽  
Alessio Crescitelli ◽  
Annalisa Lamberti ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Infrared Range ◽  
Electromagnetic Spectrum ◽  
Dna Fragments ◽  
Label Free ◽  
Large Area ◽  
Sensing Platform ◽  
Low Concentrations ◽  
Surface Enhanced ◽  
Fingerprint Region

We propose and demonstrate a sensing platform based on plasmonic metasurfaces for the detection of very low concentrations of deoxyribo-nucleic acid (DNA) fragments. The platform relies on surface-enhanced infrared absorption spectroscopy, implemented via a multispectral metasurface. Specifically, different regions (“pixels”) are engineered so as to separately cover the medium-infrared range of the electromagnetic spectrum extending from the functional-groups to the fingerprint region of a single analyte. In conjunction with a suitable bio-functionalization, this enables univocal and label-free recognition of specific molecules. For experimental validation, we fabricate a large-area gold metasurface on a silicon chip, and functionalize it with a recognition layer of peptide nucleic acid (PNA). Our experimental results indicate the possibility to detect complementary DNA fragments in concentrations as low as 50 fM, i.e., well below the value attained by standard methods, with additional advantages in terms of processing time, versatility and ease of implementation/operation.

A sensitive SERS detection of miRNA using a label-free multifunctional probe

2015 ◽  
Vol 51 (94) ◽  
pp. 16836-16839 ◽  
Author(s):  
Hao Zhang ◽  
Yu Liu ◽  
Jian Gao ◽  
Junhui Zhen
Keyword(s):  
Nucleic Acid ◽  
Raman Scattering ◽  
Detection Method ◽  
Surface Enhanced Raman Scattering ◽  
Label Free ◽  
Strand Displacement ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Enhanced Raman Scattering

A novel surface enhanced Raman scattering (SERS) detection method is fabricated for miRNA based on a smart multifunctional probe for dual cyclical nucleic acid strand-displacement polymerization (CNDP).

Nanostructure-based surface-enhanced Raman scattering biosensors for nucleic acids and proteins

2016 ◽  
Vol 4 (10) ◽  
pp. 1757-1769 ◽  
Author(s):  
Jie Chao ◽  
Wenfang Cao ◽  
Shao Su ◽  
Lixing Weng ◽  
Shiping Song ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Raman Scattering ◽  
Nucleic Acids ◽  
Protein Detection ◽  
Surface Enhanced Raman Scattering ◽  
Label Free ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Nanostructure-based SERS platforms have been developed for nucleic acid and protein detection ranging from label-free, labeled and multiplex analyses.

scholarly journals Flexible SERS Sensors Based on Carbon Nanomaterials-Supported Au Nanostructures

2021 ◽  
Vol 6 (1) ◽  
pp. 7
Author(s):  
Rong Yang ◽  
Weichen Fang ◽  
Xiao Zuo ◽  
Igor M. De Rosa ◽  
Wenbo Xin
Keyword(s):  
Single Molecule ◽  
High Performance ◽  
Carbon Nanomaterials ◽  
Label Free ◽  
Single Molecule Level ◽  
Low Concentrations ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Au Nanowires

Surface-enhanced Raman scattering (SERS) is a powerful technique to detect analytes in a label-free and non-destructive way at extremely low concentrations, even down to the single-molecule level. In the present study, a series of anisotropic Au nanostructures are integrated onto the platforms of carbon nanomaterials, mainly including carbon nanotubes (CNTs) and graphene, in order to fabricate high-performance flexible SERS sensors. Sizes, dimensions, and shapes of Au nanostructures can be well controlled through this strategy, based on which Au nanowires, nanoribbons, nanoplates, nanobelts, and nanoframes are successfully deposited onto CNT films and graphene templates, respectively. Significantly enhanced plasmonic activity originates from these Au nanocrystals, which provide increased SERS signals of the analytes by many orders of magnitude, while CNT films or graphene substrates offer superior flexibility and accessibility. For instance, A flexible SERS sensor made of graphene supported Au nanoframes can detect the analyte R6G at the concentration as low as 10−9 M. The mechanism for the sensitivity enhancement could be attributed to the homogenous distribution of Au nanoframes on the graphene support as well as the strong molecule adsorption to the graphene nanoporous network.

scholarly journals SERS Biosensor Based on Engineered 2D-Aperiodic Nanostructure for In-Situ Detection of Viable Brucella Bacterium in Complex Matrix

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 886
Author(s):  
Massimo Rippa ◽  
Riccardo Castagna ◽  
Domenico Sagnelli ◽  
Ambra Vestri ◽  
Giorgia Borriello ◽  
...  
Keyword(s):  
High Performance ◽  
Foodborne Pathogen ◽  
Surface Enhanced Raman Spectroscopy ◽  
High Performing ◽  
Sensing Platform ◽  
Food Detection ◽  
Raman Enhancement ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
In Situ Detection

Brucella is a foodborne pathogen globally affecting both the economy and healthcare. Surface Enhanced Raman Spectroscopy (SERS) nano-biosensing can be a promising strategy for its detection. We combined high-performance quasi-crystal patterned nanocavities for Raman enhancement with the use of covalently immobilized Tbilisi bacteriophages as high-performing bio-receptors. We coupled our efficient SERS nano-biosensor to a Raman system to develop an on-field phage-based bio-sensing platform capable of monitoring the target bacteria. The developed biosensor allowed us to identify Brucella abortus in milk by our portable SERS device. Upon bacterial capture from samples (104 cells), a signal related to the pathogen recognition was observed, proving the concrete applicability of our system for on-site and in-food detection.

scholarly journals Modifications of the PAMONO-Sensor Help to Size and Quantify Low Number of Individual Biological and Non-Biological Nano-Particles

2021 ◽  
Vol 6 (1) ◽  
pp. 26
Author(s):  
Rahat Morad Talukder ◽  
Al Shahriar Hossain Rakib ◽  
Julija Skolnik ◽  
Zohair Usfoor ◽  
Katharina Kaufmann ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Nano Particles ◽  
Label Free ◽  
Specific Detection ◽  
Virus Like Particles ◽  
Low Concentrations ◽  
Challenging Tasks ◽  
Image Area

In a series of recently published works, we demonstrated that the plasmon-assisted microscopy of nano-objects (PAMONO) technique can be successfully employed for the sizing and quantification of single viruses, virus-like particles, microvesicles and charged non-biological particles. This approach enables label-free, but specific detection of biological nano-vesicles. Hence, the sensor, which was built up utilizing plasmon-assisted microscopy, possesses relative versatility and it can be used as a platform for cell-based assays. However, one of the challenging tasks for such a sensor was the ability to reach a homogeneous illumination of the whole surface of the gold sensor slide. Moreover, in order to enable the detection of even relatively low concentrations of nano-particles, the focused image area had to be expanded. Both tasks were solved via modifications of previously described PAMONO-sensor set ups. Taken together, our latest findings can help to develop a research and diagnostic platform based on the principles of the surface plasmon resonance (SPR)-assisted microscopy of nano-objects.

scholarly journals Label-Free Detection of Human Serum Using Surface-Enhanced Raman Spectroscopy Based on Highly Branched Gold Nanoparticle Substrates for Discrimination of Non-Small Cell Lung Cancer

2018 ◽  
Vol 2018 ◽  
pp. 1-13
Author(s):  
Xiaowei Cao ◽  
Zhenyu Wang ◽  
Liyan Bi ◽  
Jie Zheng
Keyword(s):  
Lung Cancer ◽  
Raman Spectroscopy ◽  
Cell Lung Cancer ◽  
Surface Enhanced Raman Spectroscopy ◽  
Small Cell ◽  
Label Free ◽  
Small Cell Lung ◽  
Surface Enhanced ◽  
Different Types ◽  
Surface Enhanced Raman

Surface-enhanced Raman spectroscopy (SERS) is a good candidate for the development of fast and easy-to-use diagnostic tools, possibly used on serum in screening tests. In this study, a potential label-free serum test based on SERS spectroscopy was developed to analyze human serum for the diagnosis of the non-small cell lung cancer (NSCLC). We firstly synthesized novel highly branched gold nanoparticles (HGNPs) at high yield through a one-step reduction of HAuCl4 with dopamine hydrochloride at 60°C. Then, HGNP substrates with good reproducibility, uniformity, and high SERS effect were fabricated by the electrostatically assisted (3-aminopropyl) triethoxysilane-(APTES-) functionalized silicon wafer surface-sedimentary self-assembly method. Using as-prepared HGNP substrates as a high-performance sensing platform, SERS spectral data of serum obtained from healthy subjects, lung adenocarcinoma patients, lung squamous carcinoma patients, and large cell lung cancer patients were collected. The difference spectra among different types of NSCLC were compared, and analysis result revealed their intrinsic difference in types and contents of nucleic acids, proteins, carbohydrates, amino acids, and lipids. SERS spectra were analyzed by principal component analysis (PCA), which was able to distinguish different types of NSCLC. Considering its time efficiency, being label-free, and sensitivity, SERS based on HGNP substrates is very promising for mass screening NSCLC and plays an important role in the detection and prevention of other diseases.

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