scholarly journals Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis

2021 ◽  
Vol 9 ◽  
Author(s):  
Mansheng Li ◽  
Xiao Li ◽  
Binghui Liu ◽  
Luye Lv ◽  
Wenjuan Wang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Age Groups ◽  
Skin Aging ◽  
Epidermal Stem Cell ◽  
Dermal Matrix ◽  
Time Resolved ◽  
Skin Development ◽  
Ecm Proteins ◽  
And Function

Skin aging is a physiological issue that is still relatively poorly understood. Studies have demonstrated that the dermal extracellular matrix (ECM) plays important roles in skin aging. However, the roles of the changes in ECM characteristics and the molecules that are secreted to the extracellular space and are involved in the formation of the dermal matrix from birth to old age remain unclear. To explore the way in which the ECM microenvironment supports the functions of skin development across different age groups is also poorly understood, we used a decellularization method and matrisome analysis to compare the composition, expression, and function of the dermal ECM in toddler, teenager, adult, and elderly skin. We found that the collagens, glycoproteins, proteoglycans, and regulatory factors that support skin development and interact with these core ECM proteins were differentially expressed at different ages. ECM expression markers occurring during the process of skin development were identified. In addition, our results elucidated the characteristics of ECM synthesis, response to skin development, and the features of the ECM that support epidermal stem cell growth via the basement membrane during skin aging.

Enhanced Structure and Function of Stem Cell-Derived Beta-Like Cells Cultured on Extracellular Matrix

2020 ◽  
Author(s):  
Reena Singh ◽  
Richard Tan ◽  
Clara Tran ◽  
Thomas Loudovaris ◽  
Helen E. Thomas ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Structure And Function ◽  
And Function ◽  
Cells Cultured

scholarly journals The Effects of Ionising and Non-Ionising Electromagnetic Radiation on Extracellular Matrix Proteins

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3041
Author(s):  
Ren Jie Tuieng ◽  
Sarah H. Cartmell ◽  
Cliona C. Kirwan ◽  
Michael J. Sherratt
Keyword(s):  
Extracellular Matrix ◽  
Electromagnetic Radiation ◽  
Cell Biology ◽  
Biological Effects ◽  
Well Being ◽  
Ecm Proteins ◽  
Clinical Consequences ◽  
Radiation Induced ◽  
And Function ◽  
Cellular Components

Exposure to sub-lethal doses of ionising and non-ionising electromagnetic radiation can impact human health and well-being as a consequence of, for example, the side effects of radiotherapy (therapeutic X-ray exposure) and accelerated skin ageing (chronic exposure to ultraviolet radiation: UVR). Whilst attention has focused primarily on the interaction of electromagnetic radiation with cells and cellular components, radiation-induced damage to long-lived extracellular matrix (ECM) proteins has the potential to profoundly affect tissue structure, composition and function. This review focuses on the current understanding of the biological effects of ionising and non-ionising radiation on the ECM of breast stroma and skin dermis, respectively. Although there is some experimental evidence for radiation-induced damage to ECM proteins, compared with the well-characterised impact of radiation exposure on cell biology, the structural, functional, and ultimately clinical consequences of ECM irradiation remain poorly defined.

Regulatory effects of stem cell factor and interleukin-4 on adhesion of human mast cells to extracellular matrix proteins

Blood ◽  
2002 ◽  
Vol 99 (3) ◽  
pp. 966-972 ◽  
Author(s):  
Axel Lorentz ◽  
Detlef Schuppan ◽  
Andreas Gebert ◽  
Michael P. Manns ◽  
Stephan C. Bischoff
Keyword(s):  
Extracellular Matrix ◽  
Mast Cell ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Mast Cells ◽  
Stem Cell Factor ◽  
Collagen I ◽  
Interleukin 4 ◽  
Human Mast Cells ◽  
Ecm Proteins

Abstract Mast cells are inflammatory and immunoregulatory cells resident in tissues. They develop from bone marrow-derived progenitor cells that enter the tissue through the blood circulation. The specific localization and migration of mast cells in tissues is dependent on their interaction with extracellular matrix (ECM) proteins. Adhesion of human mast cells isolated from intestinal mucosa and cultured in the presence of stem cell factor (SCF) to ECM proteins is analyzed. It was observed that SCF is a unique cytokine enhancing mast cell adhesion to all tested ECM proteins (fibronectin, laminin, collagen I, III, IV, VI, XIV) up to 5-fold, particularly to fibronectin (54% ± 12% of mast cells) and to denatured collagens (40% ± 12% on cyanogen bromide-cleaved peptides of collagen I). Most noteworthy, preculture of mast cells with interleukin-4 (IL-4), in addition to SCF, reduced their potency to adhere to ECM proteins to one third compared to mast cells cultured with SCF alone. Mast cell adhesion was preferentially mediated by β1 integrins, and most cells expressed the ECM-binding integrins α2β1, α3β1, α4β1, α5β1, and αVβ3. SCF-induced mast cell adhesion was totally blocked by wortmannin and apigenin, indicating an involvement of phosphatidylinositol 3-kinase and mitogen-activated protein kinase, and it was related to an up-regulation of the HUTS-21 β1 epitope, which is associated with an activated conformation of β1. In conclusion, these data indicate that SCF induces the adhesion of cultured mast cells to ECM proteins, whereas IL-4 may promote detachment from the ECM.

scholarly journals Role of PGE-2 and Other Inflammatory Mediators in Skin Aging and Their Inhibition by Topical Natural Anti-Inflammatories

Cosmetics ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 6 ◽  
Author(s):  
Bryan Fuller
Keyword(s):  
Skin Cancer ◽  
Inflammatory Mediators ◽  
Normal Skin ◽  
Sun Exposure ◽  
Skin Aging ◽  
Tnf Alpha ◽  
Dermal Matrix ◽  
Chronological Aging ◽  
And Function

Human skin aging is due to two types of aging processes, “intrinsic” (chronological) aging and “extrinsic” (external factor mediated) aging. While inflammatory events, triggered mainly by sun exposure, but also by pollutants, smoking and stress, are the principle cause of rapid extrinsic aging, inflammation also plays a key role in intrinsic aging. Inflammatory events in the skin lead to a reduction in collagen gene activity but an increase in activity of the genes for matrix metalloproteinases. Inflammation also alters proliferation rates of cells in all skin layers, causes thinning of the epidermis, a flattening of the dermo-epidermal junction, an increase in irregular pigment production, and, finally, an increased incidence of skin cancer. While a large number of inflammatory mediators, including IL-1, TNF-alpha and PGE-2, are responsible for many of these damaging effects, this review will focus primarily on the role of PGE-2 in aging. Levels of this hormone-like mediator increase quickly when skin is exposed to ultraviolet radiation (UVR), causing changes in genes needed for normal skin structure and function. Further, PGE-2 levels in the skin gradually increase with age, regardless of whether or not the skin is protected from UVR, and this smoldering inflammation causes continuous damage to the dermal matrix. Finally, and perhaps most importantly, PGE-2 is strongly linked to skin cancer. This review will focus on: (1) the role of inflammation, and particularly the role of PGE-2, in accelerating skin aging, and (2) current research on natural compounds that inhibit PGE-2 production and how these can be developed into topical products to retard or even reverse the aging process, and to prevent skin cancer.

scholarly journals Extracellular Matrix as a Regulator of Epidermal Stem Cell Fate

2018 ◽  
Vol 19 (4) ◽  
pp. 1003 ◽  
Author(s):  
Elina Chermnykh ◽  
Ekaterina Kalabusheva ◽  
Ekaterina Vorotelyak
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Fate ◽  
Epidermal Stem Cell ◽  
Stem Cell Fate

Domain organizations of extracellular matrix proteins and their evolution

Development ◽  
1994 ◽  
Vol 1994 (Supplement) ◽  
pp. 35-42
Author(s):  
Jürgen Engel ◽  
Vladimir P. Efimov ◽  
Patrik Maurer
Keyword(s):  
Extracellular Matrix ◽  
Cell Signalling ◽  
Coiled Coil ◽  
Structure And Function ◽  
Matrix Proteins ◽  
Cell Binding ◽  
Ecm Proteins ◽  
Triple Helices ◽  
And Function ◽  
Polypeptide Chains

The astonishing diversity in structure and function of extracellular matrix (ECM) proteins originates from different combinations of domains. These are defined as autonomously folding units. Many domains are similar in sequence and structure indicating common ancestry. Evolutionarily homologous domains are, however, often functionally very different, which renders function prediction from sequence difficult. Related and different domains are frequently repeated in the same or in different polypeptide chains. Common assembly domains include α-helical coiled-coil domains and collagen triple helices. Other domains have been shown to be involved in assembly to other ECM proteins or in cell binding and cell signalling. The function of most of the domains, however, remains to be elucidated. ECM proteins are rather recent `inventions', and most occur either in plants or mammals but not in both. Their creation by domain shuffling involved a number of different mechanisms at the DNA level in which introns played an important role.

scholarly journals Proteome-wide and matrisome-specific alterations during human pancreas development and maturation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zihui Li ◽  
Daniel M. Tremmel ◽  
Fengfei Ma ◽  
Qinying Yu ◽  
Min Ma ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Developmental Stages ◽  
Age Groups ◽  
Immunofluorescent Staining ◽  
Pancreas Development ◽  
Human Pancreas ◽  
Proteomics Analysis ◽  
Ecm Proteins ◽  
Isobaric Tags ◽  
And Function

AbstractThe extracellular matrix (ECM) is unique to each tissue and capable of guiding cell differentiation, migration, morphology, and function. The ECM proteome of different developmental stages has not been systematically studied in the human pancreas. In this study, we apply mass spectrometry-based quantitative proteomics strategies using N,N-dimethyl leucine isobaric tags to delineate proteome-wide and ECM-specific alterations in four age groups: fetal (18-20 weeks gestation), juvenile (5-16 years old), young adults (21-29 years old) and older adults (50-61 years old). We identify 3,523 proteins including 185 ECM proteins and quantify 117 of them. We detect previously unknown proteome and matrisome features during pancreas development and maturation. We also visualize specific ECM proteins of interest using immunofluorescent staining and investigate changes in ECM localization within islet or acinar compartments. This comprehensive proteomics analysis contributes to an improved understanding of the critical roles that ECM plays throughout human pancreas development and maturation.

scholarly journals Extracellular Matrix Expression in Human Induced Pluripotent Stem Cell-Derived Optic Vesicles

2021 ◽  
pp. 1-10
Author(s):  
Heuy-Ching Wang ◽  
Ramesh R. Kaini ◽  
Christina L. Rettinger ◽  
Heuy-Ching Wang
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Pluripotent Stem Cell ◽  
Critical Role ◽  
Induced Pluripotent Stem Cell ◽  
Ecm Proteins ◽  
Optic Vesicles ◽  
Induced Pluripotent

Background: Human tissue/organ development is a complex, highly orchestrated process, regulated in part by the surrounding extracellular matrix (ECM). Every complex tissue, including the retina, has a unique ECM configuration that plays a critical role in cellular differentiation, adhesion, migration, and maturation. Aim: To characterize ECM expression of human induced pluripotent stem cell-derived optic vesicles (iPSC-OVs). Methods: A 3- dimensional (3D) in vitro suspension culture system was used to direct differentiation of human induced pluripotent stem cells (iPSCs) into optic vesicles (OVs). Stepwise differentiation of iPSCs into retinal progenitor cells was confirmed by sequential expression of OTX2, SOX1, SIX6, LHX2, PAX6, and CHX10. Expression of ECM genes in iPSC-derived OVs was analyzed by RT2 ProfilerTM PCR Array, whereas immunofluorescence staining was performed to detect ECM proteins in the OVs. Results: A number of cell adhesion molecules (CAMs) previously reported to be abundantly expressed in iPSCs such as E-cadherin, Intercellular adhesion molecule-1 (ICAM1), Integrin-α L, Integrin-α M, Integrin-α 6 were downregulated while neural and retina specific CAMs including neural cell adhesion molecule 1 (NCAM1), neural plakophilin-related armadillo repeat protein (NPRAP), Integrin-α 1 and Integrin-α 4 were upregulated. Several glycoproteins that have been reported to play key roles during retinogenesis, namely CD44, Tenascin C, Tenascin R, Neurocan, Neuroglycan C, Delta 2 Catenin, Vitronectin, and Reelin were also present. Conclusion: We have identified an array of ECM proteins that were expressed during retinogenesis. Further characterization of these proteins will lead to a better understanding of retinal development.

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