Can Inhibin B Reflect Ovarian Reserve of Healthy Reproductive Age Women Effectively?

ObjectiveThe reference range and potential value of inhibin B are still unclear and controversial. This study aimed to define the variation trend of inhibin B in healthy women with age and explore its value in the reflection of ovarian reserve.MethodsA total of 2524 healthy reproductive age women from eight medical institutes nationwide were recruited. The variation tendency of inhibin B with age was primarily established in the first group of 948 women and validated in another 605. We evaluated the relationship between inhibin B and classic ovarian reserve and function markers. The potency of inhibin B in predicting AFC <5-7 was also estimated and compared with FSH.ResultsThe nomogram showed that serum levels of inhibin B rapidly decreased after the age of 40. Inhibin B was positively correlated with AMH (R = 0.57, P < 0.001), AFC (R = 0.34, P < 0.001) and testosterone (R = 0.10, P = 0.002), and negatively correlated with FSH (R = -0.41, P < 0.001) and LH (R = -0.20, P < 0.001) and FSH/LH (R=-0.18, P < 0.001), while no correlation was found with PRL. Unexpectedly, Inhibin B (AUC = 0.74, P < 0.001 for the establishment population; AUC = 0.78, P < 0.001 for the validation population) had a slightly higher value than FSH (AUC = 0.71, P < 0.001 for the establishment population; AUC = 0.72, P < 0.001 for the validation population) in diagnosing AFC <5-7.ConclusionsFor healthy reproductive age women, the decline of inhibin B can reflect decreased ovarian reserve effectively, having a good consistency with AMH and AFC. More importantly, inhibin B had an advantage in predicting AFC <5-7 compared with FSH, which suggested the potential of inhibin B in predicting ovarian response. These results will be helpful to the clinical application of inhibin B in the evaluation of female ovarian reserve and the assessment of their reproductive capacity. Trial registration: http://clinicaltrials.gov; NCT02294500.

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P–640 High physical activity and ovarian reserve: A prospective study of normo-ovulatory professional athletes

Human Reproduction ◽

10.1093/humrep/deab130.639 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

N Miller ◽

Y Pasternak ◽

C Dornstein ◽

E Haiki. Herzberger ◽

N Zada ◽

...

Keyword(s):

Physical Activity ◽

Ovarian Reserve ◽

Inhibin B ◽

Reproductive Age ◽

Control Group ◽

Age At Menarche ◽

Physically Active ◽

High Physical Activity ◽

Reproductive Age Women ◽

Ovarian Reserve Markers

Abstract Study question Is high physical activity (HPA) associated with low ovarian reserve in normo-ovulatory, reproductive-age women? Summary answer HPA does not affect ovarian reserve negatively. What is known already HPA is associated with menstrual irregularities and subsequent potential infertility, probably through hypothalamic neuroendocrine pathways. However, it is not yet known whether HPA influences the ovarian reserves of normo-ovulatory, reproductive-age women. Study design, size, duration This observational, cross-sectional study compared 30 professional female athletes who were engaged in HPA for at least 3 years prior to study recruitment, with high International Physical Activity Questionnaire (IPAQ) scores and 30 women who did not engage in physical activity. The study was conducted at a tertiary medical center from 2017–2020. Participants/materials, setting, methods Physically active, normo-ovulatory women (n = 30), ages 20–35 years were recruited from The Wingate Institute, the Israeli National Institute for Sport Excellence. Non-physically active women (n = 30), matched by age and BMI to the HPA group, were recruited from the hospital staff. Both groups were evaluated for ovarian reserve markers on day 2–5 of the menstrual cycle, including follicular stimulating hormone (FSH), antral follicle count (AFC), anti-Mullerian hormone (AMH) and Inhibin B. Main results and the role of chance The average age of the athletes (HPA group) was 30.1±2.1 years and of the nonactive (control) group 31.6±3.8 years (p = 0.071). BMI of the 2 groups was similar (22.6±2.4 vs. 21.3±2.6; p = 0.075) for the HPA and control groups, respectively. Regarding ovarian reserve, no significant differences were observed between the HPA group and the control group with respect to FSH (p = 0.304), AFC (p = 0.27), AMH (0.507) or Inhibin B (p = 0.074). For the HPA group, older age at menarche was positively associated with AFC (p = 0.008) and AMH (p = 0.009) and not with FSH levels (p = 0.313). For the nonactive group, no significant association between age at menarche and FSH levels, AFC or AMH was found (p = 0.433, p = 0.274 and p = 0.163, respectively). Additionally, for the HPA group, duration of physical activity per week (hours) was not significantly associated with FSH levels, AFC or AMH (p = 0.619, p = 0.608 or p = 0.997, respectively). Limitations, reasons for caution Although we investigated the ovarian reserves of 30 women engaged in HPA, a larger cohort would provide more information. Information on diet and sleep habits was not evaluated and may result in some confounding. Moreover, it would be more informative if we also followed these women regarding fecundability and fertility. Wider implications of the findings: This study demonstrated that HPA may not negatively affect ovarian reserve markers. These findings may provide reassurance for women who are engaged in HPA and attempting pregnancy. Further research needs s to be confuted. Trial registration number 0247–16

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The relationship between self-report and biomarkers of stress in low-income reproductive-age women

American Journal of Obstetrics and Gynecology ◽

10.1016/j.ajog.2010.08.002 ◽

2010 ◽

Vol 203 (6) ◽

pp. 577.e1-577.e8 ◽

Cited By ~ 18

Author(s):

Ann E.B. Borders ◽

William A. Grobman ◽

Laura B. Amsden ◽

Thomas W. McDade ◽

Lisa K. Sharp ◽

...

Keyword(s):

Low Income ◽

Reproductive Age ◽

Self Report ◽

Reproductive Age Women ◽

The Relationship

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The relationship between anti-müllerian hormone and aneuploidy in reproductive-age women undergoing preimplantation genetic testing; is there a correlation?

Fertility and Sterility ◽

10.1016/j.fertnstert.2019.07.565 ◽

2019 ◽

Vol 112 (3) ◽

pp. e171-e172

Author(s):

Monica Pasternak ◽

Micha Thompson ◽

Steven Spandorfer

Keyword(s):

Genetic Testing ◽

Reproductive Age ◽

Preimplantation Genetic Testing ◽

Reproductive Age Women ◽

The Relationship

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Utility of Ovarian Reserve Screening with Anti-Müllerian Hormone for Reproductive Age Women Deferring Pregnancy

Journal of Women s Health ◽

10.1089/jwh.2016.5842 ◽

2017 ◽

Vol 26 (4) ◽

pp. 345-351 ◽

Cited By ~ 1

Author(s):

Lisa C. Grossman ◽

Lauren Zakarin Safier ◽

Melissa D. Kline ◽

Cariann W. Chan ◽

Rogerio A. Lobo ◽

...

Keyword(s):

Ovarian Reserve ◽

Reproductive Age ◽

Reproductive Age Women

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189 THE EFFECT OF VITAMIN D SUPPLEMENTATION ON ANTI-MULLERIAN HORMONE LEVELS IN REPRODUCTIVE-AGE WOMEN

Reproduction Fertility and Development ◽

10.1071/rdv27n1ab189 ◽

2015 ◽

Vol 27 (1) ◽

pp. 185 ◽

Cited By ~ 2

Author(s):

M. Taheri ◽

M. Modarres ◽

A. Abdollahi

Keyword(s):

Vitamin D ◽

Vitamin D Deficiency ◽

Intervention Group ◽

Serum Levels ◽

Vitamin D Supplementation ◽

T Test ◽

Reproductive Age ◽

Control Group ◽

Post Intervention ◽

Reproductive Age Women

Vitamin D deficiency has been correlated with the infertility and lower clinical pregnancy following IVF. Anti-Mullerian hormone (AMH) plays a key role during follicle development; it has been recognised as a predictor of regular ovulation and probably IVF success. Considering the critical need for experimental human study to investigate the impact of vitamin D supplementation on ovulatory function, the aim of this study was to demonstrate the effectiveness of the vitamin D supplementation on AMH serum levels among reproductive-age women with vitamin D deficiency. 195 reproductive women (18–35 year-old) with confirmed vitamin D deficiency [serum 25(OH)D <75 nmol L–1] and without diagnosed polycystic ovary syndrome (PCOS) were enrolled to this controlled clinical trial. Participants were randomly assigned to a control group (n = 96) or an intervention group (n = 99). Women in the intervention group used 2000 IU day–1 vitamin D drops for 15 weeks. 19 participants were missed during the follow-up; finally the numbers of women in the intervention and control groups were 91 and 85, respectively. At the beginning of the study and after the intervention, 25-hydroxyvitamin D and AMH serum levels were quantified using enzyme immunoassay (EIA; Immunodiagnostic Systems, Boldon, UK) and ELISA (Beckman-Coulter Inc., Fullerton, CA, USA) methods respectively. The post-intervention AMH measurement was performed after 2–5 weeks in the same day-of-cycle on which basal AMH measurement was done. Paired t-test, independent t-test, and Pearson correlation were used as appropriate and a P-value of less than 0.05 was considered significant. Significantly low AMH levels were seen in the vitamin D deficient women of this study (14.46 ± 11.92 pmol L–1 in control group and 14.09 ± 11.52 pmol L–1 in intervention group). After the intake of vitamin D supplementation in intervention group, AMH levels were increased to 24.89 ± 12.47 pmol L–1, which were significantly different from the 15.43 ± 13.03 pmol L–1 in control group (P < 0.001). Correlation coefficients for AMH with pre-intervention and post-intervention vitamin D were r = 0.489 and r = 0.599 respectively (P < 0.001). Treatment of vitamin D deficiency increases AMH to the optimum levels. Vitamin D deficient women had low levels of AMH. These findings support other studies which found a correlation of poor IVF outcomes with low vitamin D levels. Vitamin D supplementation could be useful in the improvement of controlled ovarian hyper-stimulation/IVF outcomes in case of vitamin D deficiency.

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Serum levels of insulin-like factor 3, anti-Müllerian hormone, inhibin B, and testosterone during pubertal transition in healthy boys: a longitudinal pilot study

Reproduction ◽

10.1530/rep-13-0435 ◽

2014 ◽

Vol 147 (4) ◽

pp. 529-535 ◽

Cited By ~ 21

Author(s):

Marie Lindhardt Johansen ◽

Ravinder Anand-Ivell ◽

Annette Mouritsen ◽

Casper P Hagen ◽

Mikkel G Mieritz ◽

...

Keyword(s):

Leydig Cell ◽

Cell Function ◽

Serum Levels ◽

Reproductive Hormones ◽

Inhibin B ◽

Testicular Volume ◽

Serum Concentrations ◽

Pubertal Onset ◽

Additional Information ◽

And Function

Insulin-like factor 3 (INSL3) is a promising marker of Leydig cell function with potentially high clinical relevance. Limited data of INSL3 levels in relation to other reproductive hormones in healthy pubertal boys exist. In this study, we aimed to evaluate longitudinal serum changes in INSL3 compared with LH, FSH, testosterone, inhibin B, and anti-Müllerian hormone (AMH) during puberty in healthy boys. Ten boys were included from the longitudinal part of the COPENHAGEN Puberty Study. Pubertal evaluation, including testicular volume, was performed and blood samples were drawn every 6 months for 5 years. Serum concentrations of testosterone were determined by a newly developed LC–MS/MS method, and serum concentrations of INSL3, AMH, inhibin B, FSH, and LH respectively were determined by validated immunoassays. The results showed that serum INSL3 levels increased progressively with increasing age, pubertal onset, and testicular volume. In six of the ten boys, LH increased before the first observed increase in INSL3. In the remaining four boys, the increase in LH and INSL3 was observed at the same examination. The increases in serum concentrations of LH, testosterone, and INSL3 were not parallel or in ordered succession and varied interindividually. We demonstrated that INSL3 concentrations were tightly associated with pubertal onset and increasing testicular volume. However, the pubertal increases in LH, INSL3, and testosterone concentrations were not entirely parallel, suggesting that INSL3 and testosterone may be regulated differently. Thus, we speculate that INSL3 provides additional information on Leydig cell differentiation and function during puberty compared with traditional markers of testicular function.

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The relationship between body mass index and anti-mullerian hormone levels in reproductive-age women; is there a negative correlation?

Fertility and Sterility ◽

10.1016/j.fertnstert.2018.02.083 ◽

2018 ◽

Vol 109 (3) ◽

pp. e42-e43 ◽

Cited By ~ 1

Author(s):

Monica C. Pasternak ◽

Paul Christos ◽

Steven D. Spandorfer

Keyword(s):

Body Mass Index ◽

Body Mass ◽

Negative Correlation ◽

Reproductive Age ◽

Hormone Levels ◽

Reproductive Age Women ◽

The Relationship

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Relationship of the ovarian reserve with autoimmune thyroid diseases in the reproductive period

Terapevticheskii arkhiv ◽

10.26442/00403660.2019.10.000095 ◽

2019 ◽

Vol 91 (10) ◽

pp. 14-18

Author(s):

O R Grigoryan ◽

N S Krasnovskaya ◽

R K Mikheev ◽

I S Yarovaia ◽

E N Andreeva ◽

...

Keyword(s):

Ovarian Reserve ◽

Inhibin B ◽

Reproductive Age ◽

Fmr1 Gene ◽

Normal Range ◽

Women Of Reproductive Age ◽

Antral Follicles ◽

Antithyroid Antibodies ◽

Healthy Women ◽

The Status

Aim. To compare ovarian reserve in healthy women of reproductive age - carriers of antithyroid antibodies (ATA) and in healthy women of reproductive age negative for ATA. Materials and methods. 70 healthy women of young reproductive age in the state of euthyroidism (from 18 to 38 years old) were examined. Participants were divided into equal groups (n=35) depending on the status of the presence of antithyroid antibodies (AT-TPO, AT-TG). On the 2nd-4th days of the menstrual cycle, the following markers of the ovarian reserve were determined: serum levels of anti-Müllerian hormone (AMG), inhibin B, FSH, LH, estradiol, testosterone and progesterone, as well as ultrasound parameters - the number of antral follicles and the volume of the ovaries. In addition, to determine the predisposition to premature ovarian failure, an analysis was performed to the number of CGG repeats in the FMR1 gene. Results and discussion. Statistically significantly differs such parameters as the level of estradiol and testosterone, while the differences were not clinically significant. All the parameters evaluated were within the normal range, the main predictors of the ovarian reserve (levels of AMG and inhibin B, the number of antral follicles) remained in the normal range. An increase in the number of repeats of CGG in the FMR1 gene was not detected in any of the participants in the study. Conclusion. In healthy young reproductive age women, the status of ATA does not have a direct effect on the ovarian reserve.

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