scholarly journals Environmentally Driven Color Variation in the Pearl Oyster Pinctada margaritifera var. cumingii (Linnaeus, 1758) Is Associated With Differential Methylation of CpGs in Pigment- and Biomineralization-Related Genes

2021 ◽  
Vol 12 ◽  
Author(s):  
Pierre-Louis Stenger ◽  
Chin-Long Ky ◽  
Céline M. O. Reisser ◽  
Céline Cosseau ◽  
Christoph Grunau ◽  
...  
Keyword(s):  
Spatial Organization ◽  
Molecular Mechanisms ◽  
Common Knowledge ◽  
Pearl Oyster ◽  
Differential Methylation ◽  
Color Variation ◽  
Epigenetic Factors ◽  
Pinctada Margaritifera ◽  
Depth Variation ◽  
Biomineralization Process

Today, it is common knowledge that environmental factors can change the color of many animals. Studies have shown that the molecular mechanisms underlying such modifications could involve epigenetic factors. Since 2013, the pearl oyster Pinctada margaritifera var. cumingii has become a biological model for questions on color expression and variation in Mollusca. A previous study reported color plasticity in response to water depth variation, specifically a general darkening of the nacre color at greater depth. However, the molecular mechanisms behind this plasticity are still unknown. In this paper, we investigate the possible implication of epigenetic factors controlling shell color variation through a depth variation experiment associated with a DNA methylation study performed at the whole genome level with a constant genetic background. Our results revealed six genes presenting differentially methylated CpGs in response to the environmental change, among which four are linked to pigmentation processes or regulations (GART, ABCC1, MAPKAP1, and GRL101), especially those leading to darker phenotypes. Interestingly, the genes perlucin and MGAT1, both involved in the biomineralization process (deposition of aragonite and calcite crystals), also showed differential methylation, suggesting that a possible difference in the physical/spatial organization of the crystals could cause darkening (iridescence or transparency modification of the biomineral). These findings are of great interest for the pearl production industry, since wholly black pearls and their opposite, the palest pearls, command a higher value on several markets. They also open the route of epigenetic improvement as a new means for pearl production improvement.

A Layered Structure in the Organic Envelopes of the Prismatic Layer of the Shell of the Pearl Oyster Pinctada margaritifera (Mollusca, Bivalvia)

2009 ◽  
Vol 16 (1) ◽  
pp. 91-98 ◽  
Author(s):  
Y. Dauphin ◽  
A. Brunelle ◽  
M. Cotte ◽  
J.P. Cuif ◽  
B. Farre ◽  
...  
Keyword(s):  
Layered Structure ◽  
Layered Structures ◽  
Pearl Oyster ◽  
Prismatic Layer ◽  
Pinctada Margaritifera ◽  
Organic Layers ◽  
Spatially Resolved ◽  
Biomineralization Process ◽  
Composition And Structure

AbstractThe organic interprismatic layers of the mollusc Pinctada margaritifera are studied using a variety of highly spatially-resolved techniques to establish their composition and structure. Our results show that both the interlamellar sheets of the nacre and interprismatic envelopes form layered structures. Additionally, these organic layers are neither homogeneous in composition, nor continuous in their structure. Both structures play a major role in the biomineralization process and act as a boundary between mineral units.

scholarly journals Transcription of Genes Involved in Biomineralization in Two Mantle Morphs of Pearl Oyster, Pinctada Persica

2021 ◽  
Author(s):  
Fatemeh Parvizi ◽  
Arash Akbarzadeh ◽  
Ahmad Farhadi ◽  
Sophie Arnaud-Haond ◽  
Mohammad Sharif Ranjbar
Keyword(s):  
Molecular Mechanisms ◽  
Soft Tissues ◽  
Active Role ◽  
Pearl Oyster ◽  
Color Variation ◽  
Mantle Tissue ◽  
Pearl Oysters ◽  
Color Morphs ◽  
Pearl Quality

Abstract A few species of mollusks display color variation in their soft tissues. In pearl oysters, the color polymorphism in mantle tissue is associated with the color and radiance of shell and pearl. The study of biomineralization related genes in mantle tissue of pearl oysters can be used as a suitable approach to better identify the molecular mechanisms that influence shell and pearl quality and color variations. In this study, we investigated the transcription of biomineralization-related genes in black and orange mantle morphotypes of pearl oyster, Pinctada persica in both warm and cool seasons using quantitative real-time PCR. Our results showed that the genes involved in biomineralization of the prismatic and nacre layer, i.e.; ASP, KRMP, MRNP34, SHELL, SHEM1B, LINKINE, PIF, SHEM5, NACREIN, and in pigmentation (TYR2A) were significantly highly expressed in orange phenotype compared to those of black one, suggesting the existence of different genetic processes between two color morphs of mantle tissue and the more active role of genes in orange morphotype. In black mantle phenotype, ASP, KRMP, SHEM5 and PIF and in the orange phenotype, only KRMP and PRISM showed difference in seasonal expression. This study provides an accurate understanding of the mantle trait of P. persica.

scholarly journals Stable DNMT3L overexpression in SH-SY5Y neurons recreates a facet of the genome-wide Down syndrome DNA methylation signature

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Benjamin I. Laufer ◽  
J. Antonio Gomez ◽  
Julia M. Jianu ◽  
Janine M. LaSalle
Keyword(s):  
Dna Methylation ◽  
Down Syndrome ◽  
Neuronal Differentiation ◽  
Molecular Mechanisms ◽  
Cpg Island ◽  
Differential Methylation ◽  
Chromosome 21 ◽  
Pairwise Comparisons ◽  
Genome Wide ◽  
A Genome

Abstract Background Down syndrome (DS) is characterized by a genome-wide profile of differential DNA methylation that is skewed towards hypermethylation in most tissues, including brain, and includes pan-tissue differential methylation. The molecular mechanisms involve the overexpression of genes related to DNA methylation on chromosome 21. Here, we stably overexpressed the chromosome 21 gene DNA methyltransferase 3L (DNMT3L) in the human SH-SY5Y neuroblastoma cell line and assayed DNA methylation at over 26 million CpGs by whole genome bisulfite sequencing (WGBS) at three different developmental phases (undifferentiated, differentiating, and differentiated). Results DNMT3L overexpression resulted in global CpG and CpG island hypermethylation as well as thousands of differentially methylated regions (DMRs). The DNMT3L DMRs were skewed towards hypermethylation and mapped to genes involved in neurodevelopment, cellular signaling, and gene regulation. Consensus DNMT3L DMRs showed that cell lines clustered by genotype and then differentiation phase, demonstrating sets of common genes affected across neuronal differentiation. The hypermethylated DNMT3L DMRs from all pairwise comparisons were enriched for regions of bivalent chromatin marked by H3K4me3 as well as differentially methylated sites from previous DS studies of diverse tissues. In contrast, the hypomethylated DNMT3L DMRs from all pairwise comparisons displayed a tissue-specific profile enriched for regions of heterochromatin marked by H3K9me3 during embryonic development. Conclusions Taken together, these results support a mechanism whereby regions of bivalent chromatin that lose H3K4me3 during neuronal differentiation are targeted by excess DNMT3L and become hypermethylated. Overall, these findings demonstrate that DNMT3L overexpression during neurodevelopment recreates a facet of the genome-wide DS DNA methylation signature by targeting known genes and gene clusters that display pan-tissue differential methylation in DS.

scholarly journals Novel Gene Regulation in Normal and Abnormal Spermatogenesis

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 666
Author(s):  
Li Du ◽  
Wei Chen ◽  
Zixin Cheng ◽  
Si Wu ◽  
Jian He ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Leydig Cells ◽  
Molecular Mechanisms ◽  
New Technologies ◽  
Genetic Regulation ◽  
Myoid Cells ◽  
Epigenetic Factors ◽  
Future Directions ◽  
New Genes ◽  
Human Spermatogenesis

Spermatogenesis is a complex and dynamic process which is precisely controlledby genetic and epigenetic factors. With the development of new technologies (e.g., single-cell RNA sequencing), increasingly more regulatory genes related to spermatogenesis have been identified. In this review, we address the roles and mechanisms of novel genes in regulating the normal and abnormal spermatogenesis. Specifically, we discussed the functions and signaling pathways of key new genes in mediating the proliferation, differentiation, and apoptosis of rodent and human spermatogonial stem cells (SSCs), as well as in controlling the meiosis of spermatocytes and other germ cells. Additionally, we summarized the gene regulation in the abnormal testicular microenvironment or the niche by Sertoli cells, peritubular myoid cells, and Leydig cells. Finally, we pointed out the future directions for investigating the molecular mechanisms underlying human spermatogenesis. This review could offer novel insights into genetic regulation in the normal and abnormal spermatogenesis, and it provides new molecular targets for gene therapy of male infertility.

scholarly journals Calcein staining of calcified structures in pearl oyster Pinctada margaritifera and the effect of food resource level on shell growth

Aquaculture ◽  
2011 ◽  
Vol 313 (1-4) ◽  
pp. 149-155 ◽  
Author(s):  
Clémentine Linard ◽  
Yannick Gueguen ◽  
Jacques Moriceau ◽  
Claude Soyez ◽  
Bélinda Hui ◽  
...  
Keyword(s):  
Shell Growth ◽  
Food Resource ◽  
Pearl Oyster ◽  
Pinctada Margaritifera ◽  
Resource Level ◽  
Effect Of Food

scholarly journals Revisiting the Organic Template Model through the Microstructural Study of Shell Development in Pinctada margaritifera, the Polynesian Pearl Oyster

Minerals ◽  
2018 ◽  
Vol 8 (9) ◽  
pp. 370 ◽  
Author(s):  
Jean-Pierre Cuif ◽  
Yannicke Dauphin ◽  
Gilles Luquet ◽  
Kadda Medjoubi ◽  
Andrea Somogyi ◽  
...  
Keyword(s):  
Pearl Oyster ◽  
Nacreous Layer ◽  
Prismatic Layer ◽  
Pinctada Margaritifera ◽  
Microstructural Study ◽  
Leading Role ◽  
Complex Process ◽  
Gathering Data

A top-down approach to the mineralized structures and developmental steps that can be separated in the shells of Pinctada margaritifera was carried out. Detailed characterizations show that each of the two major layers usually taken into account (the outer prismatic layer and the inner nacreous layer) is actually the result of a complex process during which the microstructural patterns were progressively established. From its early growing stages in the deeper part of the periostracal grove up to the formation of the most inner nacreous layers, this species provides a demonstrative case study illustrating the leading role of specifically secreted organic structures as determinants of the crystallographic properties of the shell-building units. Gathering data established at various observational scales ranging from morphology to the nanometer level, this study allows for a reexamination of the recent and current biomineralization models.

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