scholarly journals SNPs in Mammary Gland Epithelial Cells Unraveling Potential Difference in Milk Production Between Jersey and Kashmiri Cattle Using RNA Sequencing

2021 ◽  
Vol 12 ◽  
Author(s):  
Syed Mudasir Ahmad ◽  
Basharat Bhat ◽  
Shakil Ahmad Bhat ◽  
Mifftha Yaseen ◽  
Shabir Mir ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Rna Sequencing ◽  
Milk Production ◽  
High Impact ◽  
Production Traits ◽  
Milk Production Traits ◽  
Amino Acid Synthesis ◽  
Jersey Cattle ◽  
Resistance Pathway

Deep RNA sequencing experiment was employed to detect putative single nucleotide polymorphisms (SNP) in mammary epithelial cells between two diverse cattle breeds (Jersey and Kashmiri) to understand the variations in the coding regions that reflect differences in milk production traits. The low milk-producing Kashmiri cattle are being replaced by crossbreeding practices with Jersey cattle with the aim of improving milk production. However, crossbred animals are prone to infections and various other diseases resulting in unsustainable milk production. In this study, we tend to identify high-impact SNPs from Jersey and Kashmiri cows (utilizing RNA-Seq data) to delineate key pathways mediating milk production traits in both breeds. A total of 607 (442 SNPs and 169 INDELs) and 684 (464 SNPs and 220 INDELs) high-impact variants were found specific to Jersey and Kashmir cattle, respectively. Based on our results, we conclude that in Jersey cattle, genes with high-impact SNPs were enriched in nucleotide excision repair pathway, ABC transporter, and metabolic pathways like glycerolipid metabolism, pyrimidine metabolism, and amino acid synthesis (glycine, serine, and threonine). Whereas, in Kashmiri cattle, the most enriched pathways include endocytosis pathway, innate immunity pathway, antigen processing pathway, insulin resistance pathway, and signaling pathways like TGF beta and AMPK which could be a possible defense mechanism against mammary gland infections. A varied set of SNPs in both breeds, suggests a clear differentiation at the genomic level; further analysis of high-impact SNPs are required to delineate their effect on these pathways.

Restriction fragment length polymorphisms at the ornithine decarboxylase locus associated with milk protein yield in Holsteins

1998 ◽  
Vol 65 (2) ◽  
pp. 341-345
Author(s):  
JIANBO YAO ◽  
SAMUEL E. AGGREY ◽  
DAVID ZADWORNY ◽  
URS KÜHNLEIN ◽  
J. FLAN HAYES
Keyword(s):  
Growth Hormone ◽  
Mammary Gland ◽  
Growth Factors ◽  
Candidate Genes ◽  
Ornithine Decarboxylase ◽  
Milk Production ◽  
Production Traits ◽  
Milk Production Traits ◽  
Gene Markers ◽  
Ample Evidence

Marker-assisted selection may provide the opportunity to make significant genetic gains in the improvement of economically important traits in livestock (Soller & Beckmann, 1983; Smith & Simpson, 1986). Implementation of this approach will first require identification of candidate genes or anonymous gene markers associated with the traits of interest. Candidate genes are those with a known relationship between physiological or biochemical processes and an economically important trait. In dairy cattle, genes associated with mammary gland growth, development and function are excellent candidate genes for milk production traits.The polyamines are low molecular mass polycations that influence cell proliferation and growth (Tabor & Tabor, 1984; Pegg, 1986). Ornithine decarboxylase (ODC, EC 4.1.1.17) catalyses the conversion of ornithine to putrescine, the rate-limiting step in polyamine biosynthesis (Pegg, 1986). The level of ODC is induced in quiescent cells exposed to a wide variety of stimuli such as growth hormone, corticosteroids, testosterone and growth factors (Tabor & Tabor, 1984). There is ample evidence that growth factors influence morphogenesis and differentiation of the mammary gland (Imagawa et al. 1994). Trophic hormones that are associated with lactation, such as prolactin and growth hormone, are also required to induce differentiation of bovine mammary epithelial cells (Huynh et al. 1991; Flint & Gardner, 1994), and both mitogenic and lactogenic effects of prolactin, insulin and hydrocortisone appear to be mediated through the polyamine pathway (Rillema et al. 1977; Bedford & Zadworny, 1990; Golden & Rillema, 1993). Strange et al. (1992) have shown that ODC is involved in synthesis of a milk component; ODC has elevated expression in the lactating mammary gland, which declines sharply after weaning. It is therefore possible that particular variants of ODC could be associated with increased mammary gland function and thus influence milk related traits.The objectives of the present study were to estimate allelic frequencies of ODC polymorphisms in Holstein bulls and to evaluate further the genotypic effects of ODC variants on milk production traits.

scholarly journals Evaluation of associations of the polymorphism in the placentaspecific promoter 1.1 of the <i>CYP19</i> gene in Black-and-White and Jersey cattle with milk production traits (short communication)

2006 ◽  
Vol 49 (4) ◽  
pp. 311-314 ◽  
Author(s):  
M. Jędrzejczak ◽  
I. Szatkowska ◽  
S. Zych ◽  
W. Grzesiak ◽  
E. Czerniawska-Piątkowska ◽  
...  
Keyword(s):  
Milk Production ◽  
Short Communication ◽  
Production Traits ◽  
Milk Production Traits ◽  
P450 Gene ◽  
Black And White ◽  
Jersey Cattle ◽  
Pcr Rflp ◽  
Pvuii Polymorphism ◽  
The Relationship

Abstract. The relationship between the SNP of the cytochrome P450 gene (CYP19-PvuII) and milk production traits of Black-and-White and Jersey cattle were analysed. A total of 437 cows were included in the study. A PCR-RFLP was used to genotype. The frequencies of genotypes and alleles for the Black-and-White cows were as follows: 0.8985 – AA, 0.0977 – AB, 0.0038 – BB, and 0.9474 – CYP19A, 0.0526 – CYP19B. In the Jersey, all cows were genotyped as CYP19AA (no polymorphism). There weren’t any associations between CYP19-PvuII polymorphism and milk production traits of the investigated cows.

scholarly journals Association of genetic variants of bovine <i>prolactin</i> with milk production traits of Black-and-White and Jersey cattle

2005 ◽  
Vol 48 (2) ◽  
pp. 149-156 ◽  
Author(s):  
A. Dybus ◽  
W. Grzesiak ◽  
H. Kamieniecki ◽  
I. Szatkowska ◽  
Z. Sobek ◽  
...  
Keyword(s):  
Milk Production ◽  
Production Traits ◽  
Milk Production Traits ◽  
Black And White ◽  
Jersey Cattle ◽  
Prolactin Gene ◽  
Pcr Rflp ◽  
Rsai Polymorphism ◽  
White Cattle ◽  
Jersey Cows

Abstract. Associations between polymorphism localised in the third exon of the prolactin gene (PRL-RsaI) and milk xproduction traits of Black-and-White and Jersey cattle were analysed. A total of 427 cows were included in the study. PCR-RFLP method was used. The frequencies of genotypes and alleles were as follows: 0.7107 – AA, 0.2851 – AB, 0.0042 – BB; 0.8533 – PRLA and 0.1467 – PRLB for Black-and-White cattle and 0.0919 – AA, 0.4324 – AB, 0.4757 – BB; 0.3081 – PRLA and 0.6919 – PRLB for Jersey cattle. Statistically significant differences between the breeds were observed in the frequencies of genotypes and alleles. Associations between PRL-RsaI polymorphism and milk production traits of Jersey cows and lack of associations with these traits in Black-and-White cows were observed.

scholarly journals ANXA9, SLC27A3, FABP3 and FABP4 single nucleotide polymorphisms in relation to milk production traitsin Jersey cows

2010 ◽  
Vol 55 (No. 11) ◽  
pp. 463-467 ◽  
Author(s):  
H. Kulig ◽  
I. Kowalewska-Łuczak ◽  
M. Kmieć ◽  
K. Wojdak-Maksymiec C
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Milk Production ◽  
Milk Fat ◽  
Fatty Acid Transport ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Acid Transport ◽  
Milk Production Traits ◽  
Milk Traits

Milk components originating from blood plasma substrates are synthesized in epithelial cells of the mammary gland. Milk lipids are synthesized from fatty acids which bind to specific proteins &ndash; FABPs (fatty acid binding proteins). FABPs are a family of small cytoplasmic proteins; nine members of the family have been identified so far (FABP1&ndash;FABP9) (Chmurzyńska et al., 2006). Their main roles include fatty acid uptake, transport and metabolism. FABPs can modulate the fatty acid concentration in cells and therefore they affect different cellular processes, especially lipid metabolism. FABP3 and FABP4 are present in tissues with a high demand for fatty acids, such as heart muscle, skeletal muscles, lactating mammary gland, liver or adipose tissue (Roy et al., 2003). FABP3 gene was mapped to bovine chromosome 2 (Calvo et al., 2004), where QTLs affecting milk fat yield and content were described (Khatkar et al., 2004). FABP4 gene was mapped to BTA14 (Michal et al., 2006), which is very rich in QTLs for milk production traits (Khatkar et al., 2004). Fatty acid transport is assisted by the specific proteins called FATPs (fatty acid transport proteins). This protein group includes SLC27A3 (solute carrier family 27, member 3). It belongs to the family of proteins that facilitate long-chain fatty acid transport across the cytoplasmic membrane. Another protein with similar functions is ANXA9 (annexin A9), the member of Ca2+ and phospholipid-binding protein family (Calvo et al., 2006b). Genes encoding SLC27A3 and ANXA9 were mapped to chromosome 3, within the region where QTLs for milk fat content and the other milk traits have been mapped. Both of the above-mentioned genes are expressed in the mammary gland (Calvo et al., 2006b). The polymorphic sites (SNPs &ndash; single nucleotide polymorphisms) within the bovine FABP3, FABP4, SLC27A3 and ANXA9 genes were identified (Wu et al., 2005; Calvo et al., 2006b; Michal et al., 2006; Cho et al., 2008). Associations between SNPs in these genes and milk production traits in cattle have not been reported so far. However, polymorphism in the FABP4 gene has been significantly associated with carcass traits in cattle (Michal et al., 2006; Cho et al., 2008). Due to their expression sites, physiological properties and chromosomal localisation, the described genes might be considered as candidate genes for milk production traits. The aim of this study was to determine allele and genotype frequencies and to establish possible associations between the ANXA9, SLC27A3, FABP3 and FABP4 SNPs, and selected milk traits in Jersey cows.

scholarly journals ANXA9, SLC27A3, FABP3 and FABP4 single nucleotide polymorphisms in relation to milk production traits in Jersey cows

2010 ◽  
Vol 55 (No. 1) ◽  
pp. 463-467 ◽  
Author(s):  
H. Kulig ◽  
I. Kowalewska-Łuczak ◽  
M. Kmieć ◽  
K. Wojdak-Maksymiec
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Milk Production ◽  
Milk Fat ◽  
Fatty Acid Transport ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Acid Transport ◽  
Milk Production Traits ◽  
Milk Traits

Milk components originating from blood plasma substrates are synthesized in epithelial cells of the mammary gland. Milk lipids are synthesized from fatty acids which bind to specific proteins &ndash; FABPs (fatty acid binding proteins). FABPs are a family of small cytoplasmic proteins; nine members of the family have been identified so far (FABP1&ndash;FABP9) (Chmurzyńska et al., 2006). Their main roles include fatty acid uptake, transport and metabolism. FABPs can modulate the fatty acid concentration in cells and therefore they affect different cellular processes, especially lipid metabolism. FABP3 and FABP4 are present in tissues with a high demand for fatty acids, such as heart muscle, skeletal muscles, lactating mammary gland, liver or adipose tissue (Roy et al., 2003). FABP3 gene was mapped to bovine chromosome 2 (Calvo et al., 2004), where QTLs affecting milk fat yield and content were described (Khatkar et al., 2004). FABP4 gene was mapped to BTA14 (Michal et al., 2006), which is very rich in QTLs for milk production traits (Khatkar et al., 2004). Fatty acid transport is assisted by the specific proteins called FATPs (fatty acid transport proteins). This protein group includes SLC27A3 (solute carrier family 27, member 3). It belongs to the family of proteins that facilitate long-chain fatty acid transport across the cytoplasmic membrane. Another protein with similar functions is ANXA9 (annexin A9), the member of Ca2+ and phospholipid-binding protein family (Calvo et al., 2006b). Genes encoding SLC27A3 and ANXA9 were mapped to chromosome 3, within the region where QTLs for milk fat content and the other milk traits have been mapped. Both of the above-mentioned genes are expressed in the mammary gland (Calvo et al., 2006b). The polymorphic sites (SNPs &ndash; single nucleotide polymorphisms) within the bovine FABP3, FABP4, SLC27A3 and ANXA9 genes were identified (Wu et al., 2005; Calvo et al., 2006b; Michal et al., 2006; Cho et al., 2008). Associations between SNPs in these genes and milk production traits in cattle have not been reported so far. However, polymorphism in the FABP4 gene has been significantly associated with carcass traits in cattle (Michal et al., 2006; Cho et al., 2008). Due to their expression sites, physiological properties and chromosomal localisation, the described genes might be considered as candidate genes for milk production traits. The aim of this study was to determine allele and genotype frequencies and to establish possible associations between the ANXA9, SLC27A3, FABP3 and FABP4 SNPs, and selected milk traits in Jersey cows.

scholarly journals Two single nucleotide polymorphisms within bovine butyrophilin gene (BTN/HaeIII and BTN/SchI) and their association with milk performance traits in Jersey cattle

2010 ◽  
Vol 53 (5) ◽  
pp. 501-509
Author(s):  
M. Muszyńska ◽  
I. Szatkowska ◽  
W. Grzesiak ◽  
A. Dybus ◽  
D. Zaborski
Keyword(s):  
Milk Production ◽  
Fat Content ◽  
Lower Percentage ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Milk Production Traits ◽  
Jersey Cattle ◽  
Pcr Rflp ◽  
Milk Performance ◽  
Jersey Cows

Abstract. Associations between two polymorphisms localized in the seventh exon of the butyrophilin gene (BTN/HaeIII and BTN/SchI) and milk production traits of Jersey cattle were analysed. A total of 171 cows were included in the study. PCR-RFLP method was used. In both polymorphisms three genotypes were identified. Statistically significant differences between polymorphic variants and milk production traits of Jersey cows were observed. Those differences concerned the percentage fat content in milk in the case of both polymorphisms and additionally the yield of milk and protein in the case of BTN/HaeIII polymorphism. The influence of the combined genotypes (BTN/HaeIII/SchI) on the examined traits was also analysed. GGHaeIII/AGSchI combined genotype was characterized by a significantly lower percentage fat content in comparison to other combinations. Moreover, incomplete linkage between the studied polymorphic sites was observed, despite relatively small distance between them.

Effect of Breed and Some Non - Genetic Factors on Milk Production and Some Proportions of Its Chemical Components in Two Breeds of Local Sheep

2020 ◽  
Vol 10 (1) ◽  
pp. 227-231
Author(s):  
Rahman Hussein AL-Qasimi ◽  
Shatha Mohammed Abbas ◽  
Allawi L.D. AL-Khauzai
Keyword(s):  
Milk Production ◽  
Genetic Factors ◽  
Chemical Components ◽  
Lactation Period ◽  
Production Traits ◽  
Milk Production Traits ◽  
Fat Percentage ◽  
Awassi Sheep ◽  
Total Milk ◽  
Milk Components

The study was carried out on 19 ewes of local Awassi sheep and 12ewes local Arabi sheep in the Al-kafeel sheep station Karbala, to determine the effect of breed and some non-genetic factors such as (sex of the lamb, type of birth, age and weight of ewes at birth) on daily and total milk production and lactation period and some of milk components (fat, protein and lactose). The results showed that a significant effect (P <0.05) of the breed on milk production traits where Awassi sheep recorded the highest mean (0.91 kg , 101.63 kg , 104.86 day) compared to the Arabi sheep she was means (0.77 kg , 88.15 kg , 99.15 day) respectively. As well as in proportions of milk components with mean( 5.1 , 4.90 , 5.51) % respectively compared to the Arabi sheep (4.70 . 4.20 . 4.89) ewes with male lambs also exceeded superior ewes with female lambs in daily and total milk production and the lactation period the sex of the lamb did not affect the proportions of milk components the weight of the ewes had a significant effect (P <0.05) in milk production attributes with superior weight of ewes on lower ewes and did not affect the proportions of milk ingredients except for lactose. The type of birth and the age of the ewes did not have a significant effect in all the studied traits except for the superiority (P<0.05) of young ewes on age ewes in the fat percentage of milk.

An association analysis between PRL genotype and milk production traits in Italian Mediterranean river buffalo

2017 ◽  
Vol 84 (4) ◽  
pp. 430-433 ◽  
Author(s):  
Jun Li ◽  
Aixin Liang ◽  
Zipeng Li ◽  
Chao Du ◽  
Guohua Hua ◽  
...  
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Milk Fat ◽  
Fat Content ◽  
Phenotypic Variance ◽  
River Buffalo ◽  
Production Traits ◽  
Milk Production Traits ◽  
Exon 2 ◽  
Mediterranean River

This Research Communication describes the association between genetic variation within the prolactin (PRL) gene and the milk production traits of Italian Mediterranean river buffalo (Bufala mediterranea Italiana). High resolution melting (HRM) techniques were developed for genotyping 465 buffaloes. The association of genetic polymorphism with milk production traits was performed and subsequently the effects of parity and calving season were evaluated. Single nucleotide polymorphisms (SNPs) were identified at exons 2 and 5 and at introns 1 and 2. All the SNPs were in Hardy–Weinberg equilibrium, and statistical analysis showed that the polymorphism of intron1 was significantly (P < 0·05) associated with milk yield, milk protein content and peak milk yield. The average contribution of the intron1 genotype (r2intron1) to total phenotypic variance in milk production traits was 0·09, and the TT genotype showed lower values than CC and CT genotypes. A nonsynonymous SNP was identified in exon 2, which resulted in an amino acid change from arginine to cysteine. Moreover, the polymorphism of exon 2 was associated significantly with milk fat content (P < 0·05), and the buffaloes with TT genotype showed higher total fat content than the buffaloes with CT genotype. These findings provide evidence that polymorphisms of the buffalo PRL gene are associated with milk production traits and PRL can be used as a candidate gene for marker-assisted selection in Italian Mediterranean river buffalo breeding.

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