scholarly journals Comparison of C-Reactive Protein in Dried Blood Spots and Saliva of Healthy Adolescents

2021 ◽  
Vol 12 ◽  
Author(s):  
Anne-Christine Plank ◽  
Janina Maschke ◽  
Nicolas Rohleder ◽  
Peter A. Fasching ◽  
Matthias W. Beckmann ◽  
...  
Keyword(s):  
Total Sample ◽  
Dried Blood Spots ◽  
Oral Health Status ◽  
C Reactive Protein ◽  
Mean Values ◽  
Blood Spots ◽  
Reactive Protein ◽  
Non Invasive ◽  
The Mean ◽  
One Way Anova

Background/AimDetermining C-reactive protein (CRP) by non-invasive methods is of great interest for research addressing inflammation in young people. However, direct comparisons of such methods applied in children and adolescents are lacking so far. This study aimed to evaluate the association between CRP measured in dried blood spots (DBS CRP) and in saliva (sCRP), two less invasive alternatives to venipuncture, in 12- to 14-year-old adolescents. To evaluate the validity of both measurements in the context of biobehavioral studies, the potential of DBS CRP and sCRP to discriminate between defined BMI subgroups was assessed.Materials and MethodsCRP levels in DBS and saliva collected from 87 healthy adolescents (M = 13.25 years, SD = 0.30, 51.7% females) were determined using high sensitive CRP ELISA for serum and salivary CRP ELISA, respectively. Characteristics and correlation of both measurements were assessed for the total sample and for three subgroups classified by BMI percentile ranges (A: ≤ 25; B: 26–74; C: ≥ 75).ResultsIn the total sample, DBS CRP and sCRP were significantly associated (r = 0.59, p < 0.001). Splitting the sample into BMI-dependent subgroups revealed similarly strong associations of DBS CRP with sCRP for all three groups (A: r = 0.51; B: r = 0.61; C: r = 0.53). However, comparing the mean CRP values per BMI subgroup, one-way ANOVA reported significant differences for DBS CRP, but not for sCRP mean values.ConclusionsThe significant correlation of DBS CRP with sCRP was independent of the investigated BMI range groups, yet BMI-dependent distinction was only provided by DBS CRP mean values. Overall, our results suggest that DBS CRP is likely to reflect systemic inflammation more precisely. Salivary CRP can be alternatively determined in studies with adolescents when conditions require it, given the oral health status is assessed. Considering that DBS CRP and sCRP share only 35% of common variance, further studies should examine their specific validity.

scholarly journals The Influence of Short Duration Exercise on the Concentration of C-Reactive Protein and Selected Haematological and Biochemical Parameters in the Blood of German Shepherd Dogs

2017 ◽  
Vol 61 (2) ◽  
pp. 35-43 ◽  
Author(s):  
K. Goldírová ◽  
M. Fialkovičová ◽  
M. Benková ◽  
C. Tóthová ◽  
M. Harčárová
Keyword(s):  
Short Duration ◽  
Biochemical Parameters ◽  
C Reactive Protein ◽  
German Shepherd ◽  
Mean Values ◽  
Reactive Protein ◽  
Intensive Exercise ◽  
Before And After ◽  
The Mean ◽  
The Difference

Abstract Short-term intensive exercise may be associated with many short-lasting metabolic changes. These changes depend on the duration and intensity of the exercise. The aim of our study was to determine potential changes in C-reactive protein (CRP) and selected haematological and biochemical parameters in clinically healthy German shepherd dogs before short duration high-intensity exercise and 6 hours after the exercise. During the study, the dogs were subjected to the following defensive training: detaining a figurant running away from a dog (2×) and detaining with a counterattack (2×). The running distance was 200 m at a mean speed of 28 km.h-1. The investigation of haematological parameters revealed a significant decrease in the mean values of platelet haematocrit (PCT) 6 hours after the training. Significant differences in the levels determined before and after exercise were observed also for phosphorus (P) and for iron (Fe). At the same time, we observed a significant increase in the mean calcium (Ca) level and a significant decrease in sodium (Na) and chlorides (Cl). The activity of aspartate aminotransferase (AST) significantly increased after exercise. Concentrations of acute phase C-reactive proteins were increased 6 hours after exercise in comparison to those before exercise but the difference was insignificant. Although the concentration of CRP was increased insignificantly after the exercise, when interpreting concentrations of this nonspecific indicator of inflammation, one should keep in mind that intensive exercise may also affect its concentrations.

scholarly journals Abuse of Alabukun consumption: A biochemical and histomorphological study in Rattus norvegicus rats

2021 ◽  
Vol 8 (1) ◽  
pp. 037-042
Author(s):  
Emmanuel TonbraEgoro ◽  
Ikhide Godwin Ilegbedion ◽  
Prudence NkemdinimOkara
Keyword(s):  
Rattus Norvegicus ◽  
Daily Basis ◽  
Control Group ◽  
C Reactive Protein ◽  
Biochemical Finding ◽  
Mean Values ◽  
Reactive Protein ◽  
The Mean ◽  
Kidney Liver ◽  
Experimental Group

This study was aimed on the biochemical and histomorphological study of abuse in the consumption of alabukun powder in Rattus norvegicus rats. Five milliliter of blood specimen was collected into lithium heparin bottles from seven rats weighing 240±2g respectively with each of them administered with 0.2mg/kg alabukun powder daily for a period of two weeks (experimental group) while another seven rats weighing 240±2g each were not administered with alabukun powder (control group). Thereafter alanine aminotransferase, aspartate aminotransferase, urea, creatinine and C-reactive protein were measured quantitatively in both groups of rats. The mean values of all the measured biochemical parameters in Rattus norvegicus rats in the experimental group were statistically significant (p<0.05) as compared to that in the control group. This established biochemical finding was in conformity with the histomorphological examination of the kidney and liver organs. In conclusion, this study has established that administration of 0.2mg/kg of alabukun powder on daily basis for a period of two weeks on Rattus norvegicus rats may induce hepato-renal and inflammatory disorders. It is thus recommended that consumption of alabukun powder by humans should strictly be in compliance with its prescription. However, kidney, liver and inflammatory biomarkers should be quantitatively measured in humans that have abused its consumption with a view to ascertain their health status and prevent any deleterious risks.

scholarly journals Assessment of selected biochemical parameters among food hawkers along the streets of Yenagoa Bayelsa State, Nigeria

2021 ◽  
Vol 16 (3) ◽  
pp. 103-108
Author(s):  
Emmanuel Tonbra Egoro ◽  
Emmanuel Sunday Oni ◽  
Otaraku Jonathan Oye ◽  
Annabel Awele Idama
Keyword(s):  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Biochemical Parameters ◽  
Control Group ◽  
C Reactive Protein ◽  
Mean Values ◽  
Reactive Protein ◽  
Significant Difference ◽  
The Mean ◽  
Experimental Group

This study was aimed on assessment of selected biochemical parameters among food hawkers along the streets of Yenagoa, Bayelsa State, Nigeria. Five milliliter of blood specimen was collected from fifteen food hawkers with ≤ 5 years working experience (experimental group one), ≥ 5 years working experience (experimental group two) and nonfood hawkers (control group) respectively into lithium heparin anti-coagulated bottles. Thereafter alanine aminotransferase, aspartate aminotransferase, urea, creatinine and C-reactive protein were measured quantitatively. The mean values of volunteers in experimental group one showed no statistically significant difference (p>0.05) in alanine aminotransferase (7.46 ± 1.02), aspartate aminotransferase (7.02 ± 0.86), urea (7.70 ± 1.29) and creatinine (70.44 ± 3.54) as compared with the control group (7.42 ± 0.98), (6.98 ± 0.82), (7.65 ± 1.28) and (70.40 ± 3.52) respectively, but there was a statistically significant difference (p<0.05) in C-reactive protein (17.40 ± 1.98) when compared with the control group (2.70 ± 0.70). However, the mean values of food hawkers in experimental group two showed statistically significant difference (p< 0.05) in all the measured biochemical parameters 27.70±2.98, 21.40±2.06, 15.50±2.02, 110.70±3.74, 29.42±3.20 as compared with that of the control group 7.42±0.98, 6.98±0.82, 7.65±1.28, 70.40±3.52, 2.74±0.70. The volunteers in experimental groups one and two showed 13% -27% and 53% -80% abnormal values of the measured biochemical parameters respectively as compared with that of the control group. In conclusion, alanine aminotransferase, aspartate aminotransferase, urea, creatinine and C-reactive protein may be altered in food hawkers with ≥ 5 years working experience. It is therefore recommended that food hawkers in this category should go for hepato-renal and inflammatory biochemical parameters checkup occasionally in any registered and licensed Medical Laboratory Facility.

Comparison the effect of Arbidol plus Hydroxychloroquine vs Hydroxychloroquine alone in treatment of COVID-19 disease: A Randomized Clinical Trial

2021 ◽  
Vol 17 ◽  
Author(s):  
Rozita Khodashahi ◽  
Hamidreza Naderi ◽  
Amin Bojdy ◽  
Ali Akbar Heydari ◽  
Ashraf Tavanaee Sani ◽  
...  
Keyword(s):  
Controlled Trial ◽  
Hematological Parameters ◽  
Emergency Situation ◽  
Drug Regimen ◽  
C Reactive Protein ◽  
Mean Values ◽  
Reactive Protein ◽  
Randomized Controlled ◽  
The Mean ◽  
Single Blind

Background and aim: The main challenging issue about coronavirus disease 2019 (COVID-19) is the production of safe and stable vaccines, which is a very long process. Due to the emergency situation, regular and extensive screening of available and traditional drugs, which are commonly used for the treatment of similar viral diseases, can be a reasonable option. The present study aimed to compare the administration of hydroxychloroquine (HCQ) plus arbidol to the use of HCQ alone in the treatment of COVID-19 infection. Methods and Materials: This single-blind randomized controlled trial was carried out on a total of 100 patients with COVID-19 referring to the infection ward of Imam Reza Hospital in Mashhad, Iran, in 2020. The patients were randomly assigned to two HCQ alone and HCQ plus arbidol groups. Results: According to the obtained results, hematological parameters, including white blood cell count, hemoglobin level, lymphocyte count, and platelet count, improved in patients with COVID-19 after the treatment with both HCQ plus arbidol and HCQ alone (P<0.005). The mean values of the reduction time of C-reactive protein (CRP) were4.48±1.24 and 8.22±2.08 days in the arbidol and HCQ alone groups, respectively, indicating that CRP decreased faster in the arbidol group than that reported for the HCQ alone group (Z=0.-7.85; P<0.000).The mean scores of hospital stay were reported as 5.89±2.04 and 9.35±3.72 days in the arbidol and HCQ alone groups, respectively (Z=-4.31; P<0.005). All the patients in the arbidol group survived; while 6% of the subjects in the HCQ alone group died. In addition, the drug regimen was not changed for any patient, and no subject was transferred to the intensive care unit in the arbidol group. Conclusion: In summary, the administration of both arbidol and HCQ leads to the improvement of the hematological parameters. The present study introduced arbidol as an effective treatment for moderate to severe patients with COVID-19, which not only reduced the time of CRP normalization level but also decreased the hospitalization duration and mortality compared to those reported for HCQ.

scholarly journals C‐reactive protein from dried blood spots: Application to household air pollution field studies

Indoor Air ◽  
2019 ◽  
Vol 30 (1) ◽  
pp. 24-30 ◽  
Author(s):  
Bonnie N. Young ◽  
Jennifer L. Peel ◽  
Tracy L. Nelson ◽  
Annette M. Bachand ◽  
Judy M. Heiderscheidt ◽  
...  
Keyword(s):  
Air Pollution ◽  
Field Studies ◽  
Dried Blood Spots ◽  
Household Air Pollution ◽  
C Reactive Protein ◽  
Blood Spots ◽  
Reactive Protein

Ceruloplasmin is a Better Predictor of the Long-Term Prognosis Compared with Fibrinogen, CRP, and IL-6 in Patients with Severe Unstable Angina

Angiology ◽  
2008 ◽  
Vol 60 (1) ◽  
pp. 50-59 ◽  
Author(s):  
Antonios Ziakas ◽  
Stavros Gavrilidis ◽  
Efthimia Souliou ◽  
George Giannoglou ◽  
Ioannis Stiliadis ◽  
...  
Keyword(s):  
Unstable Angina ◽  
Cardiovascular Events ◽  
Time Course ◽  
C Reactive Protein ◽  
Mean Values ◽  
Reactive Protein ◽  
The Mean ◽  
Long Term Prognosis

Background. We investigated the time course and prognostic value of fibrinogen (Fib), C-reactive protein (CRP), interleukin-6 (IL-6), and ceruloplasmin (CP) in patients with severe unstable angina. Methods. All 4 substances were measured on admission and after 6, 12, 24, 48, and 72 hours, and after 7 days and 6 months in 40 patients with Braunwald's classification class IIIB unstable angina. Results. All recorded substances increased significantly; 15 patients had cardiovascular events during hospitalization and 11 patients during follow-up. The time course and the mean values of Fib, CRP, and IL-6 were similar in patients with and without complications both during hospitalization and follow-up. However, CP levels from 6 hours until 6 months were significantly higher in patients with complications during follow-up ( P < .05). Conclusions. Fib, CRP, IL-6, and CP levels alter in patients with severe unstable angina. However, only CP levels were related to 12-month follow-up prognosis.

C-reactive protein measured in dried blood spots from patients with cystic fibrosis

1991 ◽  
Vol 143 (1) ◽  
pp. 69-72 ◽  
Author(s):  
S.M. Cordon ◽  
J.S. Elborn ◽  
E.J. Hiller ◽  
D.J. Shale
Keyword(s):  
Cystic Fibrosis ◽  
Dried Blood Spots ◽  
C Reactive Protein ◽  
Blood Spots ◽  
Reactive Protein

scholarly journals C-reaktyvusis baltymas – komplikacijų po laparoskopinės storosios žarnos rezekcijos prediktorius

2014 ◽  
Vol 13 (2) ◽  
Author(s):  
Eligijus Poškus ◽  
Gintarė Andreikaitė ◽  
Ignas Karnuševičius
Keyword(s):  
Colorectal Surgery ◽  
Laparoscopic Colorectal Surgery ◽  
Surgery Complications ◽  
C Reactive Protein ◽  
Mean Values ◽  
Reactive Protein ◽  
Sample Test ◽  
The Mean ◽  
Sensitivity Specificity

TikslasĮvertinti ir nustatyti C-reaktyviojo baltymo prognozines savybes, diagnozuojant ankstyvas pooperacines komplikacijas po laparoskopinės storosios žarnos rezekcijos.Ligoniai ir metodaiTai perspektyvusis pacientų, kuriems VUL SK Pirmajame pilvo chirurgijos skyriuje atlikta laparoskopinė storosios žarnos rezekcija, tyrimas. Pagal tai, ar pacientai turėjo komplikacijų ar ne, juos suskirstėme į dvi grupes. C-reaktyviojo baltymo koncentracija kraujyje buvo matuojama antrą, ketvirtą ir šeštą pooperacinę parą. Apskaičiuoti abiejų grupių C-reaktyviojo baltymo vidurkiai minėtomis dienomis ir palyginti tarpusavyje taikant Stjudento (t) kriterijų nepriklausomoms imtims. Siekdami nustatyti šio baltymo koncentracijos slenkstinę ribą, geriausiai prognozuojančią komplikacijų išsivystymo tikimybę, apskaičiavome jo tyrimo jautrumą, specifiškumą, teigiamą ir neigiamą prognozines vertes.RezultataiTyrime dalyvavo 90 pacientų, iš jų 11 išsivystė komplikacijos. Nustatytos didžiausiu jautrumu ir neigiama prognozine verte pasižyminčios slenkstinės C-reaktyviojo baltymo koncentracijos: antrą pooperacinę dieną – 90 mmol/l, ketvirtą – 6O mmol/l, šeštą – 60 mmol/l. Taigi kiekvieną dieną galime vis tiksliau prognozuoti, ar komplikacijos išsivystys ar ne.IšvadosStebėdami C-reaktyviojo baltymo koncentraciją antrą, ketvirtą ir šeštą pooperacinę dieną, galime anksti diagnozuoti besivystančias komplikacijas.Reikšminiai žodžiai: C-reaktyvusis baltymas, laporoskopinė storosios žarnos rezekcija, pooperacinės komplikacijosC-reactive protein is a predictor of complications after elective laparoscopic colorectal surgeryEligijus Poškus, Gintarė Andreikaitė, Ignas Karnuševičius ObjectiveTo study the evaluation and determination of the predictive characteristics of C-reactive protein when diagnosing early postoperative complications after elective laparoscopic colorectal surgery.Materials and methodsA prospective study included patients who have undergone a laparoscopic colorectal surgery in 2010–2013. The patients were divided into two groups – with and without complications. C-reactive protein concentrations in their blood serum weremeasured on the 2nd, 4th and 6th day after surgery. We estimated the mean values of CRP in both groups and compared them using the independent sample test, the Student criteria (t). The sensitivity, specificity, positive and negative predictive valuesfor different CRP concentration cut-offs (40–130 mmol/l) were also calculated.ResultsNinety patients were included into the trial; 11 of them had complications. The cut-off values of CRP which had the highest sensitivity, specificity, positive and negative prognostic values were 2 POD – 90 mmol/l, 4 POD – 6O mmol/l, and 6POD –60 mmol/l. Thus, we can predict the risk of evolving complications more accurately each day after the operation.ConclusionsMeasuring CRP values on the 2nd, 4th and 6th postoperative day, we can diagnose the evolving complications early, i.e. after an elective laparoscopic colorectal surgery.Key words: C-reactive protein, laparoscopic colorectal surgery, complications

Sign in / Sign up

Export Citation Format

Share Document