scholarly journals Bioactivity of Biosilica Obtained From North Atlantic Deep-Sea Sponges

2021 ◽  
Vol 8 ◽  
Author(s):  
Olesia Dudik ◽  
Sara Amorim ◽  
Joana R. Xavier ◽  
Hans Tore Rapp ◽  
Tiago H. Silva ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Surface Layer ◽  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
North Atlantic ◽  
Deep Sea ◽  
Body Fluid ◽  
The One ◽  
Over Time ◽  
Stable Calcium

Demosponges are a well-known source of a plethora of bioactive compounds. In particular, they are able to form a skeleton by direct deposition of silica in a process catalyzed by silicatein. Herein, we isolated biosilicas from five different Atlantic deep-sea sponges Geodia atlantica (GA), Geodia barretti (GB), Stelletta normani (SN), Axinella infundibuliformis (AI), and Phakellia ventilabrum (PV) to explore the bioactivity and osteogenic capacity of its silica-based materials. We chemically characterized the isolated biosilicas and evaluated them for their bioactivity to deposit Ca and P on their surface (by immersion in simulated body fluid, SBF). GB-, SN-, AI-, and PV-based biosilicas did not generate a stable calcium phosphate (CaP) layer over time in the presence of SBF, however, the GA-derived one was able to form a CaP surface layer (at a Ca/P ratio of ∼1.7, similar to the one observed for hydroxyapatite), that was stable during the 28 days of testing. In addition, no cytotoxicity toward L929 and SaOs2 cells was observed for the GA-based biosilica up to a concentration of 10 mg/mL. Overall, the GA-based biosilica presents the characteristics to be used in the development of biomaterials for bone tissue engineering (BTE).

IN-VITRO BEHAVIOUR OF BIPHASIC CALCIUM PHOSPHATE WITH DIFFERENT RATIO OF SILICA CONTENT IN SIMULATED BODY FLUID

2015 ◽  
Vol 23 (1) ◽  
pp. 1-14
Author(s):  
Sudirman Sahid ◽  
◽  
Nor Shahida Kader Bashah ◽  
Salina Sabudin ◽  
◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Biphasic Calcium Phosphate ◽  
Silica Content

Surface-Directed Mineralization of Fibrous Collagen Scaffolds in Simulated Body Fluid for Tissue Engineering Applications

2021 ◽  
Vol 4 (3) ◽  
pp. 2514-2522
Author(s):  
Odair Bim-Júnior ◽  
Fabiana Curylofo-Zotti ◽  
Mariana Reis ◽  
Yvette Alania ◽  
Paulo N. Lisboa-Filho ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Collagen Scaffolds ◽  
Engineering Applications

Fetuin-A adsorption and stabilization of calcium carbonate nanoparticles in a simulated body fluid

2015 ◽  
Vol 3 (31) ◽  
pp. 6411-6419 ◽  
Author(s):  
Erick S. Vasquez ◽  
Janice L. Cunningham ◽  
Justin B. McMahan ◽  
C. LaShan Simpson ◽  
Keisha B. Walters
Keyword(s):  
Calcium Carbonate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Morphological Changes ◽  
Fetuin A ◽  
Over Time

Fetuin-A adsorbs onto and stabilizes CaCO3, as indicated by chemical and morphological changes. Complexed Fetuin-A/CaCO3 showed decreased size over time in a simulated body fluid indicating potential solubilization of CaCO3.

Evaluation of the Bioactivity Behavior of a 48 Wt %SiO2 Bioglass through Experiments in Simulated Body Fluid

2012 ◽  
Vol 727-728 ◽  
pp. 1238-1242 ◽  
Author(s):  
Roger Borges ◽  
Antônio Carlos da Silva ◽  
Juliana Marchi
Keyword(s):  
Corrosion Resistance ◽  
Surface Layer ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Glass Network ◽  
In Vitro Experiments ◽  
High Corrosion Resistance ◽  
Before And After ◽  
O 19

Among bioceramics materials, bioglasses which exhibits either a bioactive or resorbable behavior has been studied for many applications, such as bone substitutive and regeneration. When in contact with body fluid, the bioglasses can induce the formation of a hydroxyapatite surface layer. In this paper, we studied the bioactivity of a bioglass containing 48 wt %SiO2, 27 wt% Na2O, 19 wt % CaO and 6 wt %P2O5. After fusion and annealing, the samples were immersed in SBF for different periods, up to 14 days. The samples were characterized through XRD, DRIFT and SEM before and after bioactivity experiments. The overall results suggest the formation of a surface layer of consisting of hydroxyapatite, which was crystallized within seven days after in vitro experiments, leading to a suitable bioactivity. Moreover, the samples showed a glass network with high cohesion due to calcium addition, leading to materials with high corrosion resistance.

Effect of Electrical Polarization on the Behavior of Bioactive Glass Containing MgO and B2O3 in SBF

2006 ◽  
Vol 309-311 ◽  
pp. 333-336
Author(s):  
Emiko Amaoka ◽  
Erik Vedel ◽  
Satoshi Nakamura ◽  
Yusuke Moriyoshi ◽  
Jukka I. Salonen ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Bioactive Glass ◽  
Body Fluid ◽  
Charged Surface ◽  
Depolarization Current ◽  
Electrical Polarization ◽  
Thermally Stimulated Depolarization Current ◽  
Thermally Stimulated Depolarization ◽  
Calcium Phosphate Precipitation

We investigated the electrical polarizability of MgO and B2O3 containing bioactive glass (MBG). The MBG material with good manufacturing properties but low bioactivity was electrically polarized at a high dc field. The electrical polarizability of MBG was evaluated by thermally stimulated depolarization current (TSDC) measurements and immersion in simulated body fluid (SBF). The early precipitation of calcium phosphate on the negatively charged surface of the treated MBG demonstrated the increased bioactivity of the material and confirmed its polarizability. It is suggested that the electrical interactions between the polarized MBG and ions in SBF promoted the formation of the calcium phosphate precipitation. Accordingly, the increased bioactivity of the MBG in SBF is suggested to demonstrate the conversion of MBG into electrovector ceramics by the polarization treatment.

scholarly journals Biphasic composite of calcium phosphate-based mesoporous silica as a novel bone drug delivery system

2019 ◽  
Vol 10 (2) ◽  
pp. 455-470 ◽  
Author(s):  
Magdalena Prokopowicz ◽  
Adrian Szewczyk ◽  
Adrianna Skwira ◽  
Rafał Sądej ◽  
Gavin Walker
Keyword(s):  
Drug Delivery ◽  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Mesoporous Silica ◽  
Drug Delivery System ◽  
Delivery System ◽  
Body Fluid ◽  
Molecular Surface ◽  
Molar Ratio ◽  
Mineralization Potential

AbstractWe reported the new biphasic composites of calcium phosphate and mesoporous silica material (CaP@MSi) in the form of powders and pellets as a potential bone drug delivery system for doxycycline hydrochloride (DOX). The CaP@MSi powders were synthesized by cationic surfactant-templating method. The effects of 10, 20, and 30% CaP content in the CaP@MSi powders on the molecular surface structure, the cytotoxicity against osteoblast cells in vitro, and the mineralization potential in simulated body fluid were investigated. The CaP@MSi characterized by the highest mineralization potential (30% CaP content) were used for DOX adsorption and pelletization process. The CaP which precipitated in the CaP@MSi composites was characterized as calcium-deficient with the Ca:P molar ratio between 1.0 and 1.2. The cytotoxicity assays demonstrated that the CaP content in MSi increases osteoblasts viability indicating the CaP@MSi (30% CaP content) as the most biocompatible. The combination of CaP and MSi was an effective strategy to improve the mineralization potential of parent material. Upon immersion in simulated body fluid, the CaP of composite converted into the bone-like apatite. The obtained pellets preserved the mineralization potential of CaP@MSi and provided the prolonged 5-day DOX release. The obtained biphasic CaP@MSi composites seem to have an application potential as bone-specific drug delivery system.

Bioactivity Assessment of Apatite Nuclei-PVDF Composite Thin Films

2018 ◽  
Vol 782 ◽  
pp. 78-83
Author(s):  
Hasnat Zamin ◽  
Takeshi Yabutsuka ◽  
Shigeomi Takai
Keyword(s):  
Thin Films ◽  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Polyvinylidene Fluoride ◽  
Solution Casting ◽  
Casting Technique ◽  
Doctor Blade ◽  
Solution Casting Technique ◽  
Hydroxyapatite Formation

Particles of calcium phosphate were precipitated by raising the temperature and the pH of simulated body fluid (SBF) named Apatite Nuclei (AN). AN and polyvinylidene fluoride (PVDF) composites thin films with different weight percentages of AN in PVDF were fabricated by solution casting technique, using doctor blade method. In order to assess the bioactivity, the thin films were soaked in simulated body fluid (SBF). It was found that the film containing 30 wt.% of AN in PVDF actively induced hydroxyapatite formation in 3 days soaking period in SBF.

Biodegradable Bone Cement Using Calcium Phosphate Glass

2006 ◽  
Vol 309-311 ◽  
pp. 861-864 ◽  
Author(s):  
Byung Hyun Lee ◽  
Min Chul Kim ◽  
Kyoung Nam Kim ◽  
Kwang Mahn Kim ◽  
Seong Ho Choi ◽  
...  
Keyword(s):  
Weight Loss ◽  
Compressive Strength ◽  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Dissolution Rate ◽  
Phosphate Glass ◽  
Body Fluid ◽  
Setting Time ◽  
Ageing Test ◽  
Dynamic Assay

In preliminary ageing test, the cement using only calcium phosphate glass as power phase cracked with 1 day in simulated body fluid because of high dissolution rate of the cement. We added 30 wt% of either β-TCP or HA to 70 wt% calcium phosphate glass as powder phase to control the dissolution rate of the cement and performed in vitro ageing test in simulated body fluid by dynamic protocol as well as static protocol to confirm the possibility of controlling. Adding either β-TCP or HA to the cement increases the setting time and decreases the compressive strength. In dynamic assay, the pH of extract is maintained over 7. However, pH decreased to around 5 in static assay. Therefore, weight loss by static protocol continuously increased for 14 days, while weight loss by dynamic protocol almost saturated. In XRD patterns of ageing cements, CaO peaks appeared. CaO peak was maximized most lately in dynamic assay of the cement adding HA and within 7 days, the cement adding HA showed higher weight loss. It is indicated that CaO formed in surface of the cement hinder the dissolution of the cement. In addition, compressive strength increased when the CaO peak was maximized.

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