scholarly journals Genetic Recombination of the Mantle Color Pattern of Two Boring Giant Clam (Tridacna crocea) Strains

2021 ◽  
Vol 8 ◽  
Author(s):  
Junjie Wang ◽  
Zihua Zhou ◽  
Haitao Ma ◽  
Jun Li ◽  
Yanping Qin ◽  
...  

According to the RGB law display, the polymorphism of the giant clam mantle color pattern is through four iridocytes. The boring giant clam (Tridacna crocea) exhibits diverse mantle colors, including blue, green, purple, gold, and orange. In order to evaluate the genetic laws driving these mantle color patterns, a complete diallel cross between two color strains [blue strain (only blue iridocyte) and the yellow-green strain (yellow and green iridocytes)] was performed. Using a single-to-single mating system, two intra-strain crosses (BB and YY) and two reciprocal inter-strain crosses (BY and YB) were produced in triplicates. Higher fertilization rate and hatching rate were observed in all experimental groups, suggesting that there was no sperm–egg recognition barrier between the two strains. In the grow-out stage, the size of the reciprocal hybrids was larger than that of the two pure strains with a degree of heterosis. In addition, compared with the two pure strains, the hybrids have higher larval metamorphosis rate and higher survival rate. At 1 year of age, the mantle color pattern of pure strains showed 100% stable inheritance, while the reciprocal hybrids exhibited colorful patterns (a combination of blue, yellow, and green), suggesting that there was a genetic recombination of the mantle colors during the stable expression period. These results provide a theoretical basis for the formation of the mantle color of giant clam and its genetic segregation law, as well as provide guidance for genetic breeding of giant clams.

Aquaculture ◽  
2020 ◽  
Vol 519 ◽  
pp. 734890 ◽  
Author(s):  
Yuehuan Zhang ◽  
Zihua Zhou ◽  
Yanping Qin ◽  
Xingyou Li ◽  
Haitao Ma ◽  
...  

2010 ◽  
Vol 34 (1) ◽  
pp. 26-31
Author(s):  
Long-chun GU ◽  
Jin-bi LI ◽  
Da-hui YU ◽  
Gui-ju HUANG ◽  
Jian-ye LIU

1998 ◽  
Vol 71 (3) ◽  
pp. 193-198 ◽  
Author(s):  
Koji Nakayama ◽  
Miyuki Nishijima ◽  
Tadashi Maruyama
Keyword(s):  

2018 ◽  
Vol 13 (3) ◽  
pp. 213
Author(s):  
Jojo Subagja ◽  
Vitas Atmadi Prakoso

Ikan baung (Hemibagrus nemurus) memiliki prospek bagus untuk dikembangkan sebagai komoditas budidaya di Indonesia karena citarasa daging yang enak banyak diminati konsumen. Dewasa ini, peningkatan produksi benih dapat dilakukan melalui pemijahan buatan dengan manipulasi hormonal. Penelitian yang dilakukan bertujuan untuk mengevaluasi produksi induk ikan baung melalui pemijahan buatan dengan dosis penyuntikan GnRH-a berbeda. Penelitian dilakukan di Instalasi Riset Plasma Nutfah Perikanan Air Tawar, Cijeruk, Bogor pada bulan November 2017. Induk betina ikan baung yang digunakan berukuran 465,0 ± 71,8 g; dan induk jantan 426,3 ± 46,8 g. Induksi dilakukan dengan penyuntikan hormon GnRH-a dengan tiga dosis yang berbeda pada induk betina (0,3; 0,5; dan 0,7 mL/kg bobot badan), dan ikan jantan dengan dosis 0,4 mL/kg bobot badan. Masing-masing perlakuan terdiri atas tiga ekor induk betina sebagai ulangan. Hormon disuntikkan secara intramuskular, diberikan dua kali penyuntikan 35% dari dosis total pada penyuntikan pertama, dan 65% diberikan pada penyuntikan kedua, dengan interval waktu penyuntikan delapan jam. Parameter yang diamati yaitu jumlah telur ovulasi, derajat pembuahan, derajat penetasan, dan sintasan larva selama tujuh hari. Hasil penelitian menunjukkan bahwa dosis penyuntikan hormon GnRH-a 0,5 mL/kg pada induk ikan baung saat proses pemijahan buatan menghasilkan derajat penetasan yang lebih tinggi dibandingkan dosis 0,3 dan 0,7 mL/kg; serta sintasan larva yang lebih baik dibandingkan dosis 0,7 mL/kg. Sedangkan nilai jumlah telur yang berhasil ovulasi dan derajat pembuahan yang relatif lebih baik ditemukan pada perlakuan dosis 0,7 mL/kg dibandingkan dengan dosis 0,3 dan 0,5 mL/kg.Asian redtail catfish (Hemibagrus nemurus) has a good prospect to be developed as aquaculture commodity in Indonesia. It was proved by the demand of consumers regarding to its delicious flesh taste. Recently, efforts to increase seedling production could be implemented through artificial spawning with hormonal manipulation. This study was conducted to evaluate the production of broodstocks through artificial spawning with different doses of GnRH-a. Study was conducted at Research Institute for Freshwater Fisheries Germplasm, Cijeruk, Bogor in November, 2017. The broodstock used in this study was 465.0 ± 71.8 g for females, and 426.3 ± 46.8 g for males. Induction was performed by injection of GnRH-a hormone with three different doses on female broodstocks (0.3, 0.5 and 0.7 mL/kg of body weight), and male broodstocks with a dose of 0.4 mL/kg of body weight. Each treatment consisted of three females as replications. The hormone was injected intramuscularly, given twice injection: 35% of the total dose is given at the first injection, and the remaining 65% was given at the second injection, with an injection time interval of eight hours. The results showed the observed parameters of number of the ovulated eggs, fertilization rate, hatching rate, and larval survival for seven days, treatment dosage of 0.5 mL/kg showed better results (P<0.05) than the dosage of 0.3 mL/kg and 0.7 mL/kg. Parameters observed were number of ovulated eggs, fertility rate, hatching rate, and survival rate for seven days. The results showed that the dosage of 0.5 mL/kg GnRH-a injection to the broodstock during artificial spawning resulted in higher hatching rate compared to 0.3 and 0.7 mL/kg and better larval survival rate than the dosage of 0.7 mL/kg. Meanwhile, the number of ovulated eggs and fertility rate was relatively better in the treatment of 0.7 mL/kg compared with the dosage of 0.3 and 0.5 mL/kg.


2006 ◽  
Vol 8 (1) ◽  
pp. 87
Author(s):  
Nur Bambang Priyo Utomo ◽  
Ing Mokoginta ◽  
Lia Nurmalia

This experiment was conducted to determine the dietary n-3 fatty acid requirement for reproduction of broodstock zebrafish, Brachydanio rerio. Three isonitrogenous (39% crude protein) and isocaloric (3,260 kcal digestible energy/kg diet) practical diets, namely diets A, B, and C, with different levels of fatty acids were fed to zebrafish broodstock. The broodstock were cultivated in aquaria. Diet A contained low dosage of n-3 fatty acids (0.4%) and 2% n-6 fatty acids, while diets B and C contained 2% n-6 fatty acids, combined respectively with 1% and 1.5% n-3 fatty acids. Fish were fed ad satiation for 60 days using these diets. During feeding periode, gonade maturation stages were examined. The n-3 and n-6 fatty acids affected fecundity, fertilization rate, and hatching rate. On the other hand, fish fed on diets A, B, or C did not show any significance differences in the gonade somatic index and total Survival Rate (SR) of larvae produced. Fish fed on diet B produced the highest fecundity (616 eggs/g of fish), fertilization rate (94.6%), and hatching rate (93.8%). The total lipid content of eggs were significant, ranging from B (29.7%), C (23.7%), and A (16.1%). At a dosage of 2% n-6 fatty acids, zebrafish require 1% of dietary n-3 fatty acids in the diet for reproduction. Excess dosage of n-3 fatty acid in the diet adversely affected fecundity, fertilization rate, and hatching rate.


2021 ◽  
Vol 21 (4) ◽  
pp. 149-159
Author(s):  
Dulon Roy ◽  
Ashish Kumer Sarker ◽  
Abu Musa Mohammad Khairul Abedin ◽  
Smita Sarker ◽  
Kazi Nahida Begum ◽  
...  

An experiment was conducted to observe some biological aspects including growth, sex ratio, gonadal maturity, artificial insemination, fecundity, fertilization, hatching and larval development of O. pabda in a private hatchery. Total weight of the sampled fish varied from 1.60±0.43 to 111.12±6.83 g and length varied from 3.57±0.65 to 21.22±1.84 cm with the SGR 2.85±0.18 and ADG 0.73±0.05. The length and weight of the fish had a strong correlation (r²=0.966). Female dominance over male has been observed in the species. The male to female ratio was 1:1.48. Females were 16.8 cm in length at first maturity, while males were 16.6 cm in length. The GSI of female were ranged from 2.93 to 4.77 with the mean of 3.77±0.60 during the study period. The fecundity of O. pabda was ranged from 5675 to 19626 with the mean of 12503±4192 for the corresponding length 21.23±1.71, body weight 111.12±7.6 and gonad weight 4.19±0.87. Fertilization rate, hatching rate and larval development observed during the experiment. Scattered diagram for length-weight, fecundity-length, fecunditybody weight and fecundity-gonad weight relationship shows a perfect correlation both arithmetically and logarithmically.


2019 ◽  
pp. 3-7 ◽  
Author(s):  
Anna I. Mineykina ◽  
Lyudmila L. Bondareva ◽  
Darya V. Shumilina ◽  
Elena A. Domblides ◽  
Alexey V. Soldatenko

Relevance One of the basic directions of the cabbage crop breeding is the creation of F1 hybrids with a complex of economically valuable traits. This process is difficult and time-consuming as to get pure lines must be within 6-12 years hold inbreeding. Herewith not every line gives the desired heterotic effect that also requires additional verification. Methods Biotechnological method culture of isolated microspores in vitro, which allows in the first generation to receive a line with 100% homozygosity, was used to speed up the breeding process. Combination ability were performed in complete diallel cross on the basic morphological signs. Results Culture medium for cultivation of isolated microspores in vitro was optimized for each genotype of cabbage for the best embryoids regeneration. Maximum amount of embryoids was received on medium with pH 6.2 using ampicillin 100 mg/l and zeatin 1 mg/l: 466.7 ± 153.2 PCs/100 buds. A new source material for breeding – doubled haploid lines of cabbage was received. Lines – the best parents for F1 hybrids with high yield, compact rosette of leaves, with optimum inside and short outside cabbage stump was created. Studies have shown that optimization of breeding process in case of creation of pure lines of cabbage in 3 years with microspore culture requires to reduce the breeding process in 2 times.


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