scholarly journals Flow Cytometric Assessment of the Morphological and Physiological Changes of Listeria monocytogenes and Escherichia coli in Response to Natural Antimicrobial Exposure

2018 ◽  
Vol 9 ◽  
Author(s):  
Giacomo Braschi ◽  
Francesca Patrignani ◽  
Lorenzo Siroli ◽  
Rosalba Lanciotti ◽  
Oliver Schlueter ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Physiological Changes ◽  
Natural Antimicrobial ◽  
Flow Cytometric

scholarly journals Rapid assessment of antibiotic effects on Escherichia coli by bis-(1,3-dibutylbarbituric acid) trimethine oxonol and flow cytometry.

1997 ◽  
Vol 41 (9) ◽  
pp. 2001-2005 ◽  
Author(s):  
R I Jepras ◽  
F E Paul ◽  
S C Pearson ◽  
M J Wilkinson
Keyword(s):  
Escherichia Coli ◽  
Flow Cytometry ◽  
Membrane Potential ◽  
Rapid Assessment ◽  
Physiological Changes ◽  
Analytical Technique ◽  
E Coli ◽  
Flow Cytometric ◽  
Bacterial Cell Viability ◽  
Sensitive Analytical Technique

The effects of selected antibiotics on Escherichia coli were studied by flow cytometry with the fluorescent anionic membrane potential probe bis-(1,3-dibutylbarbituric acid) trimethine oxonol [DiBAC4(3)]. The actions of azithromycin, cefuroxime, and ciprofloxacin at five times the MIC on E. coli were compared by the traditional CFU assay and flow cytometry. Changes in viable counts of bacteria determined with DiBAC4(3) and by flow cytometry following treatment with the antibiotics showed trends similar to those found by the CFU assays. However, viable counts determined by flow cytometry following antibiotic treatment were 1 to 2 logs higher than those determined by the corresponding CFU assays. All the results obtained by flow cytometry were provided within 10 min after sampling, whereas the conventional CFU assay results took at least 18 h. The results indicated that flow cytometry is a sensitive analytical technique that can rapidly monitor the physiological changes of individual microorganisms following antibiotic action and can provide information on the mode of action of a drug. The membrane potential probe DiBAC4(3) provides a robust flow cytometric indicator for bacterial cell viability.

scholarly journals Autophagy—A Story of Bacteria Interfering with the Host Cell Degradation Machinery

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 110
Author(s):  
Anna K. Riebisch ◽  
Sabrina Mühlen ◽  
Yan Yan Beer ◽  
Ingo Schmitz
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Immune Response ◽  
Mycobacterium Tuberculosis ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Innate Immune ◽  
Intracellular Pathogens ◽  
Response Mechanism ◽  
Pathogenic Escherichia Coli

Autophagy is a highly conserved and fundamental cellular process to maintain cellular homeostasis through recycling of defective organelles or proteins. In a response to intracellular pathogens, autophagy further acts as an innate immune response mechanism to eliminate pathogens. This review will discuss recent findings on autophagy as a reaction to intracellular pathogens, such as Salmonella typhimurium, Listeria monocytogenes, Mycobacterium tuberculosis, Staphylococcus aureus, and pathogenic Escherichia coli. Interestingly, while some of these bacteria have developed methods to use autophagy for their own benefit within the cell, others have developed fascinating mechanisms to evade recognition, to subvert the autophagic pathway, or to escape from autophagy.

scholarly journals Molecular screening for the presence of Streptococcus agalactiae, Escherichia coli and Listeria monocytogenes in samples of milk from dairy herds

2015 ◽  
Vol 9 (17) ◽  
pp. 1189-1194
Author(s):  
Cunha Bustamante Filho Ivan ◽  
Fernanda Volken de Souza Claucia ◽  
Neutzling Lehn Daniel ◽  
Caroline Butzge Juliana ◽  
Pedro Kipper Joao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Streptococcus Agalactiae ◽  
Dairy Herds ◽  
Molecular Screening
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