scholarly journals Melatonin Is a Promising Silage Additive: Evidence From Microbiota and Metabolites

2021 ◽  
Vol 12 ◽  
Author(s):  
Mao Li ◽  
Renlong Lv ◽  
Lidong Zhang ◽  
Xuejuan Zi ◽  
Hanlin Zhou ◽  
...  
Keyword(s):  
Acid Metabolism ◽  
Diversity Indices ◽  
Control Group ◽  
Nucleic Acid Metabolism ◽  
Total Acid ◽  
Silage Fermentation ◽  
Rrna Sequencing ◽  
Long Time ◽  
Fermentation Quality ◽  
Silage Quality

The safe and effective storage of forage are very important. As an important storage method, ensiling can keep fresh forage for a long time with less nutritional loss. Melatonin has antioxidant and bacteriostasis, usually used as a natural preservative. The influence of melatonin on silage microbial or fermentation quality has not been clarified. In the present study, we aimed to clarify whether melatonin affected stylo (Stylosanthes guianensis) silage quality via microbiota and metabolites. Melatonin addition significantly improved the silage fermentation quality, including the increased contents of lactic acid and total acid (244.18–255.81% and 63.95–78.97%, respectively), as well as the decreased in pH and butyric acid content compare with control group. Moreover, 16S rRNA sequencing indicated that melatonin addition enhanced the silage microbial diversity indices (such as increase in Shannon indices but decrease in Simpson indices), and significantly shaped the composition of silage microbiota (such as increased abundances of Pantoea, Stenotrophomonas, Sphingobacterium, and Pseudomonas, and decreased abundance of Weissella). Melatonin addition also dramatically affected the metabolites of sylo silage, such as raised malonic acid and some amino acid metabolism(glycine, threonine, methionine and ornithine), while reduced nucleic acid metabolism(2-deoxyuridine and thymine) and carbon metabolism(allose and 2-deoxy-D-glucose). Collectively, our results confirmed that the lowest melatonin addition (5 mg/kg) could improve the fermentation quality, and the potential mechanisms might be associated with the microbiota and metabolites in stylo.

scholarly journals Effects of seeding rate, fertilizing time and fertilizer type on yield, nutritive value and silage quality of whole-crop wheat

2021 ◽  
Vol 9 (2) ◽  
pp. 225-234
Author(s):  
Liuxing Xu ◽  
Zhaohong Xu ◽  
Mingxia Chen ◽  
Jianguo Zhang
Keyword(s):  
Significant Interaction ◽  
Nutritive Value ◽  
Seedling Stage ◽  
Seeding Rate ◽  
Compound Fertilizer ◽  
Silage Fermentation ◽  
Fermentation Quality ◽  
Silage Quality ◽  
Jointing Stage

Whole-crop wheat (WCW) is rich in nutrients and is widely used as a forage crop. This study consisted of 2 experiments: Experiment 1 studied the yield, nutritive value and silage quality of WCW at 3 seeding rates (320 kg/ha, S320; 385 kg/ha, S385; and 450 kg/ha, S450) and different fertilizing times, i.e. 60% at seedling stage and the remaining 40% at the jointing stage vs. heading stage; and Experiment 2 examined the yield, nutritive value and silage quality of WCW receiving different fertilizer types, i.e. urea, compound fertilizer (N:P:K) and urea + compound fertilizer (all iso-nitrogenous). With the increased seeding rate, dry matter (DM) and crude protein (CP) yields tended to increase, but relative feed value tended to decrease. Experiment 1: there was no significant interaction between time of applying the second fertilizer dose and seeding rate in terms of concentrations of CP, crude fiber, ether extract, crude ash, nitrogen-free extract, neutral detergent fiber (NDF) and acid detergent fiber (ADF) in wheat (P>0.05). However, a significant interaction between fertilizing time and seeding rate was observed in terms of silage fermentation quality (pH, lactic acid, butyric acid and NH3-N concentrations) (P<0.05). Experiment 2: DM yield, CP yield and concentrations of CP, ADF and water-soluble carbohydrate were not affected by fertilizer type (P>0.05). Fertilizer type had significant effects on pH of silage and concentrations of organic acids (except propionic acid) and NH3-N in WCW silage (P<0.05). Under the present study conditions, considering DM yield, nutrient composition and silage fermentation quality, an optimal seeding rate of wheat for forage appears to be about 385 kg/ha. N fertilizer should be applied at the seedling stage and jointing stage. Although applying a mixture of urea and compound fertilizer had no significant effects on yield and nutritive value of WCW relative to applying urea alone, it did improve silage fermentation quality. Results may differ on different soils.

scholarly journals Determining Potential Feed Value and Silage Quality of Guar Bean (Cyamopsis tetragonoloba) Silages

2019 ◽  
Vol 14 (1) ◽  
pp. 342-348
Author(s):  
Mustafa Olfaz ◽  
Unal Kilic ◽  
Oguzhan Yavrucu
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Production ◽  
Control Group ◽  
Cyamopsis Tetragonoloba ◽  
Silage Fermentation ◽  
Lactic Acid Content ◽  
Feed Value ◽  
Silage Quality

AbstractThis study was carried out to determine the effects of some additives on the potential nutritional value and silage quality of guar bean (Cluster bean = Cyamopsis tetragonoloba) silages. It was hypothesized that the use of cereal grains, molasses and ecomass will reduce silage fermentation pH by increasing lactic acid production and positively improve CP content and silage quality. Four different silage groups were established; (control, grain (5%), molasses (10%) and ecomass+molasses (10%+5%)). Fresh guar beans were thoroughly mixed with the additives to homogenize, then ensiled and opened after 60 days. The results of this study revealed that guar bean silages could be used as an alternative forage in ruminant feeding because of its higher protein content (13.88%), forage feed value and silage quality. The use of molasses (GSM) and molasses + ecomass (GSEM) as additives has significantly (P ≤0.01) increased the silage quality and feed value compared to the control group. It was observed that GSM and GSEM silage groups had the highest values in terms of lactic acid content. In conclusion, guar silage can be used as an alternative feed for ruminants, but the doses of barley and molasses should be correct and tested in in vivo studies.

scholarly journals The effect of a mixture of Lactobacillus strains on silage quality and nutritive value of grass harvested at four growth stages and ensiled for two periods

2013 ◽  
Vol 22 (1) ◽  
pp. 115-126 ◽  
Author(s):  
Johan De Boever ◽  
Elien Dupon ◽  
Eva Wambacq ◽  
Joos Latré
Keyword(s):  
Cell Walls ◽  
Dry Matter ◽  
Nutritive Value ◽  
Growth Stages ◽  
Silage Fermentation ◽  
Aerobic Stability ◽  
Fermentation Quality ◽  
Silage Quality

 The effect of adding an inoculant containing Lactobacillus buchneri, L. plantarum and L. casei to wilted perennial ryegrass, harvested at four growth stages and ensiled for either 60 or 150 d on silage fermentation quality, chemical composition, rumen degradability of neutral detergent fibre (NDF) and organic matter (OM) and in vitro OM digestibility (OMd) was studied. Compared to the control silage, more sugars were fermented to lactic and acetic acid with the inoculant, resulting in a lower pH, less dry matter losses and protein degradation and a better aerobic stability. The effects of the additive on fermentation quality were more pronounced after 150 than after 60 d of ensiling, because the quality of the control silage was worse after long ensiling period. The treatment lowered NDF content of grass harvested at the first two growth stages by degrading cell walls to complex sugars, but had no effect on NDF degradability of the silage. The inoculant had no effect on rumen OM degradability nor on OMd after the short ensiling period, but increased the rumen OM degradability for the first two growth stages and OMd for all growth stages after long ensiling period.

scholarly journals Isolation, Identification and Utilization of Lactic Acid Bacteria from Silage in a Warm and Humid Climate Area

2021 ◽  
Author(s):  
chao Peng ◽  
Wentao Sun ◽  
Xiang Dong ◽  
Lili Zhao ◽  
Jun Hao
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Rhamnosus ◽  
Control Treatment ◽  
Control Group ◽  
Humid Climate ◽  
Rrna Sequencing ◽  
Fermentation Quality ◽  
Alfalfa Silage

Abstract The study aimed to isolate and identify lactic acid bacteria (LAB) from silages and their application to improve the fermentation quality of alfalfa. Forty-nine LAB strains were isolated from silages, and two strains were screened for growth and acid production rates. Then two strains were selected for Physiological and morphological tests and 16S rRNA sequencing. They were Gram-positive and catalase-negative and were able to grow at pH 3.5 and at 45°C, were unable to grow different NaCl concentrations 3, 6.5%. Strain BDy3-10 was identified as Lactobacillus rhamnosus, while TSy1-3 was identified as L. buchneri. The selected strains were evaluated on fermentation of alfalfa silage. The highest CP content occurred in the BDy3-10 treatment group. The contents of NDF and ADF in the TSy1-3 treatment were significantly lower than other treatment (P < 0.05). Compared to the control treatment, inoculation treatments deceased pH during ensiling (P < 0.001) and provided the most increased LA content after ensiling for 10 days (P < 0.001). The AA contents of all the inoculation groups were significantly increased (P < 0.001) during ensiling, and were lower than that of control group (P < 0.001). So, the TSy1-3 treatment most effectively improved the fermentation quality of alfalfa silage in warm and humid climate area.

EFFECT OF L-CYSTEINE ON α-AMYLASE PRODUCTION AND NUCLEIC ACID METABOLISM IN BACILLUS SUBTILIS

1963 ◽  
Vol 9 (3) ◽  
pp. 337-341 ◽  
Author(s):  
MICHIO OISHI ◽  
SHIGERU KITAYAMA ◽  
HAJIME TAKAHASHI ◽  
BUNJI MARUO
Keyword(s):  
Bacillus Subtilis ◽  
Nucleic Acid ◽  
Acid Metabolism ◽  
Nucleic Acid Metabolism ◽  
Amylase Production

Alteration of intrapancreatic serotonin, homocysteine, TNF-α, and NGF levels as predisposing factors for diabetes following exposure to 900-MHz waves

2021 ◽  
pp. 074823372110226
Author(s):  
Gholamali Jelodar ◽  
Mansour Azimzadeh ◽  
Fatemeh Radmard ◽  
Narges Darvishhoo
Keyword(s):  
Cell Damage ◽  
Pancreatic Beta Cell ◽  
Predisposing Factors ◽  
Male Rats ◽  
Control Group ◽  
Predisposing Factor ◽  
Time Exposure ◽  
Tnf Α ◽  
Long Time ◽  
Short Time

Exposure to mobile phone radiation causes deleterious health effects on biological systems. The objects of this study were to investigate the effect of 900-MHz radiofrequency waves (RFW) emitted from base transceiver station antenna on intrapancreatic homocysteine (Hcy), tumor necrosis factor-α (TNF-α), and nerve growth factor (NGF) as predisposing factors involved in pancreatic beta cell damage. Thirty male rats (Sprague-Dawley, 200 ± 10 g) were randomly divided into the control (without any exposure) and exposed groups: short time (2 h/day), long time (4 h/day), and exposed to 900-MHz RFW for 30 consecutive days. On the last days of the experiment, animals were killed and pancreas tissue was dissected out for evaluation of serotonin, Hcy, TNF-α, and NGF. There was a significant decrease in the serotonin and NGF levels in the pancreatic tissue of exposed groups compared to the control group ( p < 0.05). Also, the levels of serotonin and NGF in the long-time exposure were significantly lower than the short-time exposure ( p < 0.05). However, levels of Hcy and TNF-α were significantly increased in the pancreas of exposed groups compared to the control groups ( p < 0.05). Exposure to 900-MHz RFW decreased pancreatic NGF and serotonin levels and increased the proinflammatory markers (Hcy and TNF-α), which can be a predisposing factor for type 2 diabetes.

scholarly journals Sulfasalazine modifies metabolic profiles and enhances cisplatin chemosensitivity on cholangiocarcinoma cells in in vitro and in vivo models

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Malinee Thanee ◽  
Sureerat Padthaisong ◽  
Manida Suksawat ◽  
Hasaya Dokduang ◽  
Jutarop Phetcharaburanin ◽  
...  
Keyword(s):  
Redox Regulation ◽  
Treated Group ◽  
Control Group ◽  
High Recurrence Rate ◽  
Nucleic Acid Metabolism ◽  
In Vivo Models ◽  
Tryptophan Degradation ◽  
Xenograft Mice

Abstract Background Sulfasalazine (SSZ) is widely known as an xCT inhibitor suppressing CD44v9-expressed cancer stem-like cells (CSCs) being related to redox regulation. Cholangiocarcinoma (CCA) has a high recurrence rate and no effective chemotherapy. A recent report revealed high levels of CD44v9-positive cells in CCA patients. Therefore, a combination of drugs could prove a suitable strategy for CCA treatment via individual metabolic profiling. Methods We examined the effect of xCT-targeted CD44v9-CSCs using sulfasalazine combined with cisplatin (CIS) or gemcitabine in CCA in vitro and in vivo models and did NMR-based metabolomics analysis of xenograft mice tumor tissues. Results Our findings suggest that combined SSZ and CIS leads to a higher inhibition of cell proliferation and induction of cell death than CIS alone in both in vitro and in vivo models. Xenograft mice showed that the CD44v9-CSC marker and CK-19-CCA proliferative marker were reduced in the combination treatment. Interestingly, different metabolic signatures and significant metabolites were observed in the drug-treated group compared with the control group that revealed the cancer suppression mechanisms. Conclusions SSZ could improve CCA therapy by sensitization to CIS through killing CD44v9-positive cells and modifying the metabolic pathways, in particular tryptophan degradation (i.e., kynurenine pathway, serotonin pathway) and nucleic acid metabolism.

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