scholarly journals Comparative Proteomics Demonstrates Altered Metabolism Pathways in Cotrimoxazole- Resistant and Amikacin-Resistant Klebsiella pneumoniae Isolates

2021 ◽  
Vol 12 ◽  
Author(s):  
Chunmei Shen ◽  
Ying Shen ◽  
Hui Zhang ◽  
Maosuo Xu ◽  
Leqi He ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Tricarboxylic Acid Cycle ◽  
Proteome Analysis ◽  
Bioinformatic Analysis ◽  
Protein Quantification ◽  
Glutathione Metabolism ◽  
Resistant Bacteria ◽  
Drug Pressure ◽  
Altered Metabolism

Antibiotic resistance (AMR) has always been a hot topic all over the world and its mechanisms are varied and complicated. Previous evidence revealed the metabolic slowdown in resistant bacteria, suggesting the important role of metabolism in antibiotic resistance. However, the molecular mechanism of reduced metabolism remains poorly understood, which inspires us to explore the global proteome change during antibiotic resistance. Here, the sensitive, cotrimoxazole-resistant, amikacin-resistant, and amikacin/cotrimoxazole -both-resistant KPN clinical isolates were collected and subjected to proteome analysis through liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). A deep coverage of 2,266 proteins were successfully identified and quantified in total, representing the most comprehensive protein quantification data by now. Further bioinformatic analysis showed down-regulation of tricarboxylic acid cycle (TCA) pathway and up-regulation of alcohol metabolic or glutathione metabolism processes, which may contribute to ROS clearance and cell survival, in drug-resistant isolates. These results indicated that metabolic pathway alteration was directly correlated with antibiotic resistance, which could promote the development of antibacterial drugs from “target” to “network.” Moreover, combined with minimum inhibitory concentration (MIC) of cotrimoxazole and amikacin on different KPN isolates, we identified nine proteins, including garK, uxaC, exuT, hpaB, fhuA, KPN_01492, fumA, hisC, and aroE, which might contribute mostly to the survival of KPN under drug pressure. In sum, our findings provided novel, non-antibiotic-based therapeutics against resistant KPN.

Selective infection of maize roots by streptomycin-resistant Azospirillum lipoferum and other bacteria

1979 ◽  
Vol 25 (11) ◽  
pp. 1264-1269 ◽  
Author(s):  
Johanna Döbereiner ◽  
Vera Lucia Divan Baldani
Keyword(s):  
Antibiotic Resistance ◽  
Rhizosphere Soil ◽  
Resistant Bacteria ◽  
Root Infection ◽  
Azospirillum Lipoferum ◽  
Wheat Roots ◽  
Low Level ◽  
Maize Roots ◽  
Resistant Strains

The percentage of low-level streptomycin-resistant (20 μg/mL) bacteria in surface-sterilized or washed maize roots was more than a thousand times higher than that in soil populations. There was also a higher incidence of resistant bacteria in rhizosphere as compared with non-rhizosphere soil and bacteria isolated from maize roots were relatively tolerant to several other antibiotics. Azospirillum lipoferum was predominant in surface-sterilized roots of field-grown maize and was low-level streptomycin-resistant while most soil isolates were sensitive. Inoculation with A. brasilense isolated from wheat roots was unsuccessful in terms of establishment even when streptomycin-resistant strains were used. Unidentified causes of specific plant–bacteria affinities therefore transcend the role of antibiotic resistance in maize root infection.

scholarly journals What is the role of the environment in the emergence of novel antibiotic resistance genes? – A modelling approach

2021 ◽  
Author(s):  
Johan Bengtsson-Palme ◽  
Viktor Jonsson ◽  
Stefanie Heß
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Quantitative Data ◽  
Antibiotic Resistance Gene ◽  
Antibiotic Resistance Genes ◽  
Resistant Bacteria ◽  
Minor Role ◽  
Model Framework ◽  
Animal Pathogens

AbstractIt is generally accepted that intervention strategies to curb antibiotic resistance cannot solely focus on human and veterinary medicine but must also consider environmental settings. While the environment clearly has a role in the transmission of resistant bacteria, it is less clear what role it plays in the emergence of novel types of resistance. It has been suggested that the environment constitutes an enormous recruitment ground for resistance genes to pathogens, but the extent to which this actually happens is unknown. In this study, we built a model framework for resistance emergence and used the available quantitative data on the relevant processes to identify the steps which are limiting the appearance of antibiotic resistance determinants in human or animal pathogens. We also assessed the effect of uncertainty in the available data on the model results. We found that in a majority of scenarios, the environment would only play a minor role in the emergence of novel resistance genes. However, the uncertainty around this role is enormous, highlighting an urgent need of more quantitative data to understand the role of the environment in antibiotic resistance development. Specifically, more data is most needed on the fitness costs of antibiotic resistance gene (ARG) carriage, the degree of dispersal of resistant bacteria from the environment to humans, but also the rates of mobilization and horizontal transfer of ARGs. Quantitative data on these processes is instrumental to determine which processes that should be targeted for interventions to curb development and transmission of resistance.

scholarly journals Resistance to antibiotics in Gram-negative bacteria isolated from broiler carcasses

2002 ◽  
Vol 54 (1) ◽  
pp. 1-7
Author(s):  
M.A.S. Moreira ◽  
C.A. Moraes
Keyword(s):  
Antibiotic Resistance ◽  
Resistant Bacteria ◽  
Processing Plant ◽  
Growth Promoter ◽  
Gram Negative Bacteria ◽  
Beta Lactams ◽  
Gram Negative ◽  
Low Levels

One hundred and ninety-seven isolates of Gram-negative bacteria, comprising 10 genera, were isolated from poultry carcasses at a processing plant in order to investigate resistance to low levels of antibiotics. The samples were taken just after evisceration and before inspection. Most of the isolates were of Samonella and Escherichia. Other genera present were Enterobacter, Serratia, Klebsiella, Kluyvera, Erwinia, Citrobacter, Pseudomonas and Aeromonas. Distinct profiles of antibiotic resistance were detected. Resistance to more than two antibiotics predominated and spanned several classes of antibiotics. Salmonellae and escherichiae were mainly resistant to the aminoglycosides, followed by tetracycline, nitrofuran, sulpha, macrolide, chloramphenicol, quinolones and beta-lactams. Most isolates were sensitive to 30mug/ml olaquindox, the growth promoter in use at the time of sampling. However, many were resistant to a level of 10mug/ml and 13mug/ml olaquindox, levels present in the gut due to the dilution in the feed. The results suggest a possible role of low level administration of antibiotics to broilers in selecting multi-resistant bacteria in vivo.

scholarly journals Wildlife Is Overlooked in the Epidemiology of Medically Important Antibiotic-Resistant Bacteria

2019 ◽  
Vol 63 (8) ◽  
Author(s):  
Monika Dolejska ◽  
Ivan Literak
Keyword(s):  
Public Health ◽  
Antibiotic Resistance ◽  
Resistant Bacteria ◽  
Wild Animals ◽  
Antibiotic Resistant ◽  
The Public ◽  
Potential Risks ◽  
Novel Approaches ◽  
Human Animal

ABSTRACT Wild animals foraging in the human-influenced environment are colonized by bacteria with clinically important antibiotic resistance. The occurrence of such bacteria in wildlife is influenced by various biological, ecological, and geographical factors which have not yet been fully understood. More research focusing on the human-animal-environmental interface and using novel approaches is required to understand the role of wild animals in the transmission of antibiotic resistance and to assess potential risks for the public health.

A PCR-DGGE approach to evaluate the impact of wastewater source on the antibiotic resistance diversity in treated wastewater effluent

2012 ◽  
Vol 65 (7) ◽  
pp. 1323-1331 ◽  
Author(s):  
J. Sigala ◽  
A. Unc
Keyword(s):  
Antibiotic Resistance ◽  
Treatment Plant ◽  
Primary Treatment ◽  
Treated Wastewater ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Source Type ◽  
The Impact

Increased incidence of antibiotics in human-affected environments is raising concerns about increase in acquired antibiotic resistance by environmental bacteria. Wastewater collection and treatment systems are likely significant anthropogenic sinks and vectors for antibiotics and associated antibiotic resistance. Typical municipal treatment plants collect wastewaters of various sources, including well-established antibiotic resistance reservoirs such as hospitals, intensive care units and nursing homes, and integrate them with sources not commonly identified as major sources of antibiotic resistance, such as residential or industrial sources. A comprehensive PCR-DGGE diversity analysis of wastewater antibiotic-resistant bacteria was performed to evaluate the role of various wastewater sources in the discharge of antibiotic resistance by a municipal treatment plant. Wastewater sources are clearly inducing resistance in the final effluent but the role of each source type is highly variable, likely as a function of variable environmental conditions or water use patterns. Comparisons between primary treatment and secondary treatment stages indicate a strong role of the intensity of the wastewater treatment in the diversity profiles of antibiotic-resistant bacteria. While pervasiveness of antibiotic resistance in the system impedes clear discrimination between sources in the tested system, there are indications of specific source type related impacts.

The relationship between the phageome and human health: are bacteriophages beneficial or harmful microbes?

2021 ◽  
pp. 1-14
Author(s):  
L. Fernández ◽  
A.C. Duarte ◽  
A. Rodríguez ◽  
P. García
Keyword(s):  
Antibiotic Resistance ◽  
Human Health ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Phage Therapy ◽  
Negative Impact ◽  
Antibiotic Resistance Genes ◽  
Resistant Bacteria ◽  
Potential Risks

In the context of the global antibiotic resistance crisis, bacteriophages are increasingly becoming promising antimicrobial agents against multi-resistant bacteria. Indeed, a huge effort is being made to bring phage-derived products to the market, a process that will also require revising the current regulations in order to facilitate their approval. However, despite the evidence supporting the safety of phages for humans, the general public would still be reluctant to use ‘viruses’ for therapeutic purposes. In this scenario, we consider that it is important to discuss the role of these microorganisms in the equilibrium of the microbiota and how this relates to human health. To do that, this review starts by examining the role of phages as key players in bacterial communities (including those that naturally inhabit the human body), modulating the species composition and contributing to maintain a ‘healthy’ status quo. Additionally, in specific situations, e.g. an infectious disease, bacteriophages can be used as target-specific antimicrobials against pathogenic bacteria (phage therapy), while being harmless to the desirable microbiota. Apart from that, incipient research shows the potential application of these viruses to treat diseases caused by bacterial dysbiosis. This latter application would be comparable to the use of probiotics or prebiotics, since bacteriophages can indirectly improve the growth of beneficial bacteria in the gastrointestinal tract by removing undesirable competitors. On the other hand, possible adverse effects do not appear to be an impediment to promote phage therapy. Nonetheless, it is important to remember their potentially negative impact, mainly concerning their immunogenicity or their potential spread of virulence and antibiotic resistance genes, especially by temperate phages. Overall, we believe that phages should be largely considered beneficial microbes, although it is paramount not to overlook their potential risks.

scholarly journals Studies of antibiotic resistance within the patient, hospitals and the community using simple mathematical models

1999 ◽  
Vol 354 (1384) ◽  
pp. 721-738 ◽  
Author(s):  
D. J. Austin ◽  
R. M. Anderson
Keyword(s):  
Antibiotic Resistance ◽  
Mathematical Models ◽  
Treated Patient ◽  
Model Development ◽  
Resistant Bacteria ◽  
Drug Pressure ◽  
Model Framework ◽  
Hospital Size ◽  
Immunological Responses ◽  
Rate Of Spread

The emergence of antibiotic resistance in a wide variety of important pathogens of humans presents a worldwide threat to public health. This paper describes recent work on the use of mathematical models of the emergence and spread of resistance bacteria, on scales ranging from within the patient, in hospitals and within communities of people. Model development starts within the treated patient, and pharmacokinetic and pharmacodynamic principles are melded within a framework that mirrors the interaction between bacterial population growth, drug treatment and the immunological responses targeted at the pathogen. The model helps identify areas in which more precise information is needed, particularly in the context of how drugs influence pathogen birth and death rates (pharmacodynamics). The next area addressed is the spread of multiply drug–resistant bacteria in hospital settings. Models of the transmission dynamics of the pathogen provide a framework for assessing the relative merits of different forms of intervention, and provide criteria for control or eradication. The model is applied to the spread of Vancomycin–resistant enterococci in an intensive care setting. This model framework is generalized to consider the spread of resistant organisms between hospitals. The model framework allows for heterogeneity in hospital size and highlights the importance of large hospitals in the maintenance of resistant organisms within a defined country. The spread of methicillin resistant Staphylococcus aureus (MRSA) in England and Wales provides a template for model construction and analysis. The final section addresses the emergence and spread of resistant organisms in communities of people and the dependence on the intensity of selection as measured by the volume or rate of drug use. Model output is fitted to data for Finland and Iceland and conclusions drawn concerning the key factors determining the rate of spread and decay once drug pressure is relaxed.

scholarly journals Manure as a Potential Hotspot for Antibiotic Resistance Dissemination by Horizontal Gene Transfer Events

2020 ◽  
Vol 7 (3) ◽  
pp. 110 ◽  
Author(s):  
Tiago Lima ◽  
Sara Domingues ◽  
Gabriela Jorge Da Silva
Keyword(s):  
Antibiotic Resistance ◽  
Large Scale ◽  
Antibiotic Resistance Genes ◽  
Agricultural Practices ◽  
Resistant Bacteria ◽  
Antibiotic Residues ◽  
Antibiotic Resistant ◽  
Increasing Demand ◽  
Abundance And Diversity

The increasing demand for animal-derived foods has led to intensive and large-scale livestock production with the consequent formation of large amounts of manure. Livestock manure is widely used in agricultural practices as soil fertilizer worldwide. However, several antibiotic residues, antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria are frequently detected in manure and manure-amended soils. This review explores the role of manure in the persistence and dissemination of ARGs in the environment, analyzes the procedures used to decrease antimicrobial resistance in manure and the potential impact of manure application in public health. We highlight that manure shows unique features as a hotspot for antimicrobial gene dissemination by horizontal transfer events: richness in nutrients, a high abundance and diversity of bacteria populations and antibiotic residues that may exert a selective pressure on bacteria and trigger gene mobilization; reduction methodologies are able to reduce the concentrations of some, but not all, antimicrobials and microorganisms. Conjugation events are often seen in the manure environment, even after composting. Antibiotic resistance is considered a growing threat to human, animal and environmental health. Therefore, it is crucial to reduce the amount of antimicrobials and the load of antimicrobial resistant bacteria that end up in soil.

scholarly journals Escaping from ESKAPE. Clinical Significance and Antibiotic Resistance Mechanisms in Acinetobacter baumannii: a Review

2020 ◽  
Vol 11 (1) ◽  
pp. 8190-8203
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Nosocomial Infections ◽  
Antimicrobial Agents ◽  
Genetic Resistance ◽  
Resistance Mechanisms ◽  
Genomic Islands ◽  
Resistant Bacteria ◽  
Clinical Settings

Antibiotic resistance represents a critical threat in clinical settings nowadays, with an essential ecological dimension. Due to the involvement of the resistance genes, this phenomenon has gained an unprecedented expansion. Their accumulation and dissemination are facilitated by mobile genetic elements (MGEs) (plasmids, transposons, integrons, genomic islands) that can increase intracellular DNA mobility. In clinical settings, one of the critical resistant bacteria associated with nosocomial infections is Acinetobacter baumannii. This Gram-negative bacterium exhibits variate resistance mechanisms that enable it to survive in extreme environmental conditions and to evade antimicrobial agents. The enormous adaptive capacity and the essential role in the emergence of severe nosocomial infections lead to the need to study more deeply the mechanisms involved in antibiotic resistance in A. baumannii strains. In this review, we will initially present the role of A. baumannii in human and veterinary infectious pathology. We will subsequently discuss the main genetic resistance mechanisms (both intrinsic and acquired) encountered in A. baumannii strains.

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