Taxonomic Structure of Rhizosphere Bacterial Communities and Its Association With the Accumulation of Alkaloidal Metabolites in Sophora flavescens

Plant secondary metabolites (SMs) play a crucial role in plant defense against pathogens and adaptation to environmental stresses, some of which are produced from medicinal plants and are the material basis of clinical efficacy and vital indicators for quality evaluation of corresponding medicinal materials. The influence of plant microbiota on plant nutrient uptake, production, and stress tolerance has been revealed, but the associations between plant microbiota and the accumulation of SMs in medicinal plants remain largely unknown. Plant SMs can vary among individuals, which could be partly ascribed to the shift in microbial community associated with the plant host. In the present study, we sampled fine roots and rhizosphere soils of Sophora flavescens grown in four well-separated cities/counties in China and determined the taxonomic composition of rhizosphere bacterial communities using Illumina 16S amplicon sequencing. In addition, the association of the rhizosphere bacterial microbiota with the accumulation of alkaloids in the roots of S. flavescens was analyzed. The results showed that S. flavescens hosted distinct bacterial communities in the rhizosphere across geographic locations and plant ages, also indicating that geographic location was a larger source of variation than plant age. Moreover, redundancy analysis revealed that spatial, climatic (mean annual temperature and precipitation), and edaphic factors (pH and available N and P) were the key drivers that shape the rhizosphere bacterial communities. Furthermore, the results of the Mantel test demonstrated that the rhizosphere bacterial microbiota was remarkably correlated with the contents of oxymatrine, sophoridine, and matrine + oxymatrine in roots. Specific taxa belonging to Actinobacteria and Chloroflexi were identified as potential beneficial bacteria associated with the total accumulation of matrine and oxymatrine by a random forest machine learning algorithm. Finally, the structural equation modeling indicated that the Actinobacteria phylum had a direct effect on the total accumulation of matrine and oxymatrine. The present study addresses the association between the rhizosphere bacterial communities and the accumulation of alkaloids in the medicinal plant S. flavescens. Our findings may provide a basis for the quality improvement and sustainable utilization of this medicinal plant thorough rhizosphere microbiota manipulation.

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Medicinal Plants and Their Bacterial Microbiota: A Review on Antimicrobial Compounds Production for Plant and Human Health

Pathogens ◽

10.3390/pathogens10020106 ◽

2021 ◽

Vol 10 (2) ◽

pp. 106

Author(s):

Lara Mitia Castronovo ◽

Alberto Vassallo ◽

Alessio Mengoni ◽

Elisangela Miceli ◽

Patrizia Bogani ◽

...

Keyword(s):

Medicinal Plants ◽

Fungal Endophytes ◽

Bioactive Molecules ◽

Antimicrobial Compounds ◽

Bacterial Endophytes ◽

Popular Medicine ◽

Bacterial Microbiota ◽

Host Defense System ◽

Plant Microbiota

Medicinal plants (MPs) have been used since antiquity in traditional and popular medicine, and they represent a very important source of bioactive molecules, including antibiotic, antiviral, and antifungal molecules. Such compounds are often of plant origin, but in some cases, an origin or a modification from plant microbiota has been shown. Actually, the research continues to report the production of bioactive molecules by plants, but the role of plant–endophytic interaction is emerging. Classic examples are mainly concerned with fungal endophytes; however, it has been recently shown that bacterial endophytes can also play an important role in influencing the plant metabolism related to the synthesis of bioactive compounds. In spite of this, a deep investigation on the power of MP bacterial endophytes is lacking. Here, an overview of the studies on MP bacterial microbiota and its role in the production of plant antimicrobial compounds contributing to prime host defense system and representing a huge resource for biotech and therapeutic applications is provided.

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Characterization of the Plant-Associated Bacterial Microbiota of the Mexican Medicinal Species Bouvardia ternifolia

Proceedings ◽

10.3390/proceedings2020066034 ◽

2021 ◽

Vol 66 (1) ◽

pp. 34

Author(s):

Loan Edel Villalobos-Flores ◽

Samuel David Espinosa-Torres ◽

Fernando Hernández-Quiroz ◽

Alberto Piña-Escobedo ◽

Yair Cruz-Narváez ◽

...

Keyword(s):

Medicinal Plant ◽

Bacterial Communities ◽

Plant Tissues ◽

Medicinal Species ◽

Potential Source ◽

Bacterial Microbiota ◽

Rdna Sequencing ◽

Medicinal Properties ◽

Therapeutic Compounds

Though therapeutic compounds can be extracted directly from medicinal plant tissues; it is now known that many phytotherapeutic compounds are actually produced by associated microorganisms or due to their interaction with the plant. Bouvardia ternifolia is a medicinal plant that can be considered a potential source of therapeutic compounds, such as the antitumoral molecule bouvardin. We characterized the endophytic, endophytic+epiphyte and soil bacterial microbiota in the flower, leaves, stems and roots in specimens of this plant through 16S rDNA sequencing. We found that Proteobacteria followed by Actinobacteria were the most abundant bacteria phyla in this plant. On the other hand, the most representative genera living endophytically were Propionibacterium, Paraccocus, Lactobacillus, Kaistobacter, Methylobacterium and Erwinia. This study provides evidence of the composition and diversity of the bacterial communities present in soil and tissues of B. ternifolia which can be considered for subsequent analysis and understanding of the therapeutic compounds that give this species its medicinal properties.

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ETHNOVETERINARY MEDICINAL USES OF SOME MEDICINAL PLANTS ON PNEUMONIA BY THE GUJJAR AND PAHARI TRIBES OF POONCH DISTRICT OF JAMMU AND KASHMIR

FLORA AND FAUNA ◽

10.33451/florafauna.v23i2pp263-266 ◽

2017 ◽

Vol 23 (2) ◽

Author(s):

JAMIL AHMED KHAN ◽

RAJINDER PAUL

Keyword(s):

Health Care ◽

Medicinal Plants ◽

Medicinal Plant ◽

Natural Resources ◽

Indigenous People ◽

Health Care Facilities ◽

Jammu And Kashmir ◽

Medicinal Uses ◽

Modern Health Care ◽

Care Facilities

Poonch district of Jammu and Kashmir is a reservoir of enormous natural resources including the wealth of medicinal plants. The present paper deals with 12 medicinal plant species belonging to 8 genera of angiosperms used on pneumonia in cattle such as cows, sheep, goats and buffaloes in different areas of Poonch district. Due to poverty and nonavailability of modern health care facilities, the indigenous people of the area partially or fully depend on surrounding medicinal plants to cure the different ailments of their cattles. Further research on modern scientific line is necessary to improve their efficacy, safety and validation of the traditional knowledge.

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NAMING OF BASOTHO MEDICINAL PLANTS: SEMANTIC CONNECTION TO THEIR REMEDIES

Southern African Journal for Folklore Studies ◽

10.25159/1016-8427/541 ◽

2015 ◽

Vol 25 (1) ◽

Cited By ~ 1

Author(s):

R Possa ◽

P Khotso

Keyword(s):

Medicinal Plants ◽

Medicinal Plant ◽

Indigenous Knowledge ◽

Future Generation ◽

Speech Community ◽

Traditional Medicines ◽

Traditional Medicinal Plants ◽

The Future ◽

Economic Sphere ◽

Medical Plants

The indigenous knowledge of the Basotho makes it simple for this speech community to name their traditional medicinal plants in such a way that they are meaningful; this could also be viewed as an empowerment technique, especially in the economic sphere. Their medicinal plants names seem to be idiomatic and to express certain philosophies of the Basotho society. Creativity is observable in this kind of naming, and many names allude to the kind of remedy that is associated with the medicinal plant. It is therefore the interest of this paper to consider the names of medical plants among the Basotho whose names allude to the remedy they provide. The names of Sesotho medicinal plants and the reasoning of the Basotho in general behind the name and the use of each medicinal plant will be discussed in this article. This paper will further preserve and promote the use of Basotho traditional medicines for the future generation.

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Isolation of Rhizosphere Bacterial Communities from Soil

BIO-PROTOCOL ◽

10.21769/bioprotoc.1569 ◽

2015 ◽

Vol 5 (16) ◽

Cited By ~ 5

Author(s):

Laura White ◽

Volker Br�zel ◽

Senthil Subramanian

Keyword(s):

Bacterial Communities ◽

Rhizosphere Bacterial Communities

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Medicinal Plant Extracts

Plants ◽

10.3390/plants10050838 ◽

2021 ◽

Vol 10 (5) ◽

pp. 838

Author(s):

Laura Grațiela Vicaș ◽

Mariana Eugenia Mureșan

Keyword(s):

Medicinal Plants ◽

Medicinal Plant ◽

Plant Extracts ◽

Important Data ◽

Therapeutic Benefits ◽

Medicinal Plant Extracts

The therapeutic benefits of medicinal plants are well known and have been collected as important data on ethnomedicine [...]

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Climatic Zone and Soil Properties Determine the Biodiversity of the Soil Bacterial Communities Associated to Native Plants from Desert Areas of North-Central Algeria

Microorganisms ◽

10.3390/microorganisms9071359 ◽

2021 ◽

Vol 9 (7) ◽

pp. 1359

Author(s):

Elisa Bona ◽

Nadia Massa ◽

Omrane Toumatia ◽

Giorgia Novello ◽

Patrizia Cesaro ◽

...

Keyword(s):

Soil Properties ◽

Bacterial Communities ◽

Arid Environment ◽

Climatic Zone ◽

Indigenous Plants ◽

North Central ◽

The North ◽

Desert Areas ◽

Plant Microbiota ◽

Semi Arid

Algeria is the largest country in Africa characterized by semi-arid and arid sites, located in the North, and hypersaline zones in the center and South of the country. Several autochthonous plants are well known as medicinal plants, having in common tolerance to aridity, drought and salinity. In their natural environment, they live with a great amount of microbial species that altogether are indicated as plant microbiota, while the plants are now viewed as a “holobiont”. In this work, the microbiota of the soil associated to the roots of fourteen economically relevant autochthonous plants from Algeria have been characterized by an innovative metagenomic approach with a dual purpose: (i) to deepen the knowledge of the arid and semi-arid environment and (ii) to characterize the composition of bacterial communities associated with indigenous plants with a strong economic/commercial interest, in order to make possible the improvement of their cultivation. The results presented in this work highlighted specific signatures which are mainly determined by climatic zone and soil properties more than by the plant species.

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