scholarly journals Exploiting Beneficial Pseudomonas spp. for Cannabis Production

2022 ◽  
Vol 12 ◽  
Author(s):  
Carole Balthazar ◽  
David L. Joly ◽  
Martin Filion
Keyword(s):  
Plant Growth ◽  
Environmental Sustainability ◽  
Cannabis Sativa ◽  
Production Systems ◽  
Growth Promotion ◽  
Production Costs ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Naturally Occurring ◽  
The Many

Among the oldest domesticated crops, cannabis plants (Cannabis sativa L., marijuana and hemp) have been used to produce food, fiber, and drugs for thousands of years. With the ongoing legalization of cannabis in several jurisdictions worldwide, a new high-value market is emerging for the supply of marijuana and hemp products. This creates unprecedented challenges to achieve better yields and environmental sustainability, while lowering production costs. In this review, we discuss the opportunities and challenges pertaining to the use of beneficial Pseudomonas spp. bacteria as crop inoculants to improve productivity. The prevalence and diversity of naturally occurring Pseudomonas strains within the cannabis microbiome is overviewed, followed by their potential mechanisms involved in plant growth promotion and tolerance to abiotic and biotic stresses. Emphasis is placed on specific aspects relevant for hemp and marijuana crops in various production systems. Finally, factors likely to influence inoculant efficacy are provided, along with strategies to identify promising strains, overcome commercialization bottlenecks, and design adapted formulations. This work aims at supporting the development of the cannabis industry in a sustainable way, by exploiting the many beneficial attributes of Pseudomonas spp.

scholarly journals Physiological, Biochemical and Chlorophyll Fluorescence Parameters of Physalis Peruviana L. Seedlings Exposed to Different Short-Term Waterlogging Periods and Fusarium Wilt Infection

Agronomy ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 213 ◽  
Author(s):  
Cristhian C. Chávez-Arias ◽  
Sandra Gómez-Caro ◽  
Hermann Restrepo-Díaz
Keyword(s):  
Chlorophyll Fluorescence ◽  
Plant Growth ◽  
Photosynthetic Pigments ◽  
Abiotic And Biotic Stresses ◽  
Chlorophyll Fluorescence Parameters ◽  
Biotic Stresses ◽  
Fluorescence Parameters ◽  
Physalis Peruviana ◽  
Progress Curve ◽  
Cape Gooseberry

Cape gooseberry has coped with abiotic and biotic stresses such as prolonged waterlogging periods and vascular wilt in recent years. The aim of this study was to evaluate the influence of four waterlogging periods on stomatal conductance (gs), leaf water potential (Ψwf), plant growth, leaf photosynthetic pigments, malondialdehyde (MDA) production, proline content and chlorophyll fluorescence parameters in cape gooseberry plants infected with Fusarium oxysporum f. sp. physali (Foph). Two-month-old ecotype “Colombia” plants were arranged in a completely randomized factorial design in eight treatments: plants without waterlogging (control), plants with waterlogging for 4, 6 and 8 d with and without Foph, respectively. The area under the disease progress curve was higher in inoculated plants subjected to 6 and 8 d of waterlogging (55.25 and 64.25) compared to inoculated plants but without waterlogging (45.25). The results also showed a lower plant growth, gs, Ψwf, leaf photosynthetic pigments and chlorophyll fluorescence parameters (Fv/Fm, electron transport rate (ETR), Y (II) and qP) as waterlogging periods in plants with Foph increased. However, this group of plants showed a greater proline and malondialdehyde (MDA) accumulation and a higher NPQ. In conclusion, cape gooseberry shows a low acclimation to waterlogging conditions of more than 6 d in soils with Foph.

scholarly journals Plant growth promotion of barley (Hordeum vulgare L.) by potassium solubilizing bacteria with multifarious plant growth promoting attributes

2021 ◽  
Vol 8 (sp1) ◽  
pp. 17-24
Author(s):  
Tanvir Kaur ◽  
Rubee Devi ◽  
Divjot Kour ◽  
Ashok Yadav ◽  
Ajar Nath Yadav
Keyword(s):  
Plant Growth ◽  
Environmental Sustainability ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Sugar Content ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Hordeum Vulgare L ◽  
Plant Growth Promoting ◽  
Growth Promoting

Potassium (K) is the foremost macronutrients for growth of plant, soil health and fertility. The huge application of NPK chemical fertilizers negatively impacts the economy and is a threat to environmental sustainability. The rapid depletion of K mineral in soil is due to the application of agrochemicals agricultural fields for the production of crops in India. In present investigation, K-solubilizing microbes (KSM) were isolated and enumerated from cereal crops growing in Sirmour Himachal Pradesh. A total 125 bacteria were isolated and screened for K- solubilization on Aleksandrov agar plates and found that 31 bacterial strains exhibited K-solubilization. These 31 K-solubilizing strains of bacteria were additionally screened for other plant growth promoting (PGP) potential including solubilization of minerals, production of siderophores, ammonia, hydrogen cyanide and indole acetic acids. The performance of an efficient K-solubilizer was evaluated for plant growth promoting ability in pot assay under in vitro conditions. The strain EU-LWNA-25 positively influenced shoot length, fresh weight, carotenoids and total sugar content than the full dose, half dose and control. The strain enhancing physiological and growth parameters was identified by BLASTn analysis as Pseudomonas gessardii EU-LWNA-25. K-solubilizing plant growth promoting bacteria could be suitable bioinoculants for Rabi seasonal crops and overcomes the challenges of sustainable agriculture in K-deficient soil.

scholarly journals Interactions Between Bacillus Spp., Pseudomonas Spp. and Cannabis sativa Promote Plant Growth

2021 ◽  
Vol 12 ◽  
Author(s):  
Dominique Comeau ◽  
Carole Balthazar ◽  
Amy Novinscak ◽  
Nadia Bouhamdani ◽  
David L. Joly ◽  
...  
Keyword(s):  
Plant Growth ◽  
Cannabis Sativa ◽  
Growth Promotion ◽  
Genomic Analysis ◽  
Plant Growth Promoting Rhizobacteria ◽  
Growth And Yield ◽  
Promoting Effect ◽  
Rhizosphere Microbiome ◽  
Growth Promoting ◽  
The Impact

Plant growth-promoting rhizobacteria (PGPR) deploy several mechanisms to improve plant health, growth and yield. The aim of this study was to evaluate the efficacy of two Pseudomonas spp. strains and three Bacillus spp. strains used as single treatments and in consortia to improve the yield of Cannabis sativa and characterize the impact of these treatments on the diversity, structure and functions of the rhizosphere microbiome. Herein, we demonstrate a significant C. sativa yield increase up to 70% when inoculated with three different Pseudomonas spp./Bacillus spp. consortia but not with single inoculation treatments. This growth-promoting effect was observed in two different commercial soil substrates commonly used to grow cannabis: Promix and Canna coco. Marker-based genomic analysis highlighted Bacillus spp. as the main modulator of the rhizosphere microbiome diversity and Pseudomonas spp. as being strongly associated with plant growth promotion. We describe an increase abundance of predicted PGPR metabolic pathways linked with growth-promoting interactions in C. sativa.

Interaction among endophytic bacteria, sweet sorghum (Sorghum bicolor) cultivars and chemical nitrogen fertilization

2020 ◽  
Author(s):  
Gabriela Heijo ◽  
Cecilia Taulé ◽  
Cintia Mareque ◽  
Adriano Stefanello ◽  
Emanuel M Souza ◽  
...  
Keyword(s):  
Plant Growth ◽  
Nitrogen Fertilization ◽  
Environmental Sustainability ◽  
Sweet Sorghum ◽  
Endophytic Bacteria ◽  
Production Systems ◽  
N Fertilization ◽  
Plant Growth Promoting Bacteria

Abstract The application of new agricultural technologies to attain sustainable production systems is necessary. The use of plant growth-promoting bacteria to improve plant growth and health has been studied for decades. This work aimed to isolate diazotrophic endophytic bacteria associated with sweet sorghum plants and study the interaction of their inoculation in combination with chemical N-fertilization on different sorghum cultivars. A bacterial collection of 181 isolates was constructed and characterized in vitro and in vivo. From that, the strains Enterobacter sp. UYSB89 and Kosakonia sp. UYSB139 were nifH+, produce IAA, defined as true endophytes and able to promote growth of two sweet sorghum under greenhouse conditions. The evaluated cultivars responded differentially to bacterial inoculation, the nitrogen fertilization doses and their interaction. Thus, plant growth is a multifactorial consequence of the interrelation between crop practices and the plant genotypes. This knowledge is a valuable factor in terms of understanding plant-bacteria endophyte interactions to preserve environmental sustainability during the implementation of agronomic practices.

scholarly journals Genome-wide analysis of the WRKY gene family in the cucumber genome and transcriptome-wide identification of WRKY transcription factors that respond to biotic and abiotic stresses

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Chunhua Chen ◽  
Xueqian Chen ◽  
Jing Han ◽  
Wenli Lu ◽  
Zhonghai Ren
Keyword(s):  
Transcription Factors ◽  
Plant Growth ◽  
Biotic Stress ◽  
Abiotic Stresses ◽  
Expression Patterns ◽  
Wrky Transcription Factors ◽  
Biotic And Abiotic Stresses ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Cucumber Genome

Abstract Background Cucumber (Cucumis sativus L.) is an economically important vegetable crop species. However, it is susceptible to various abiotic and biotic stresses. WRKY transcription factors play important roles in plant growth and development, particularly in the plant response to biotic and abiotic stresses. However, little is known about the expression pattern of WRKY genes under different stresses in cucumber. Results In the present study, an analysis of the new assembly of the cucumber genome (v3.0) allowed the identification of 61 cucumber WRKY genes. Phylogenetic and synteny analyses were performed using related species to investigate the evolution of the cucumber WRKY genes. The 61 CsWRKYs were classified into three main groups, within which the gene structure and motif compositions were conserved. Tissue expression profiles of the WRKY genes demonstrated that 24 CsWRKY genes showed constitutive expression (FPKM > 1 in all samples), and some WRKY genes showed organ-specific expression, suggesting that these WRKYs might be important for plant growth and organ development in cucumber. Importantly, analysis of the CsWRKY gene expression patterns revealed that five CsWRKY genes strongly responded to both salt and heat stresses, 12 genes were observed to be expressed in response to infection from downy mildew and powdery mildew, and three CsWRKY genes simultaneously responded to all treatments analysed. Some CsWRKY genes were observed to be induced/repressed at different times after abiotic or biotic stress treatment, demonstrating that cucumber WRKY genes might play different roles during different stress responses and that their expression patterns vary in response to stresses. Conclusions Sixty-one WRKY genes were identified in cucumber, and insight into their classification, evolution, and expression patterns was gained in this study. Responses to different abiotic and biotic stresses in cucumber were also investigated. Our results provide a better understanding of the function of CsWRKY genes in improving abiotic and biotic stress resistance in cucumber.

scholarly journals Genetic and nutritional diversity of Bacillus subtilis isolates demonstrating different aspects related to plant growth promotion

2020 ◽  
pp. 880-888
Author(s):  
Bianca de Melo Silveira dos Santos ◽  
Maura Santos dos Reis de Andrade Silva ◽  
Davy William Hidalgo Chávez ◽  
Everlon Cid Rigobelo
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Agricultural Practices ◽  
Production Costs ◽  
Control Treatment ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Promote Plant Growth

Currently, agricultural practices have been undergoing intense transformations, imposing major challenges such as maintaining productivity with lower production costs and environmental impacts. One of the alternatives to meet these requirements is the use of plant growth promoting bacteria, including Bacillus subtilis. However, different isolates may express different aspects and levels of plant growth promotion. The present study aimed to verify the genetic and nutritional diversity of eight B. subtilis isolates, demonstrating different aspects and levels of plant growth promotion. Eight B. subtilis isolates were analyzed as to their nutritional diversity by BiologEcoPlate TM kit, genetic diversity by Box-PCR, and a trial in greenhouse conditions. The experimental design in greenhouse trial was completely randomized with 9 treatments and five replicates, resulting in 45 pots. Treatments were eight Bacillus subtilis strains, and a control treatment using plants without bacterial inoculation. Isolates 290 and 287 are genetically similar, while isolates 248 and 263 also showed similarity. Genetic and substrate consumption (carbon) analyses showed differences and similarities among isolates, allowing the distribution of isolates into different groups. It was observed that the isolate with the highest ability to promote plant growth was the only isolate that consumed glycyl-L- glutamic acid. These results open the way for further investigations in an attempt to clarify what are the conditions and / or characteristics required by isolates for the plant growth promotion to be more effective.

scholarly journals Application of the bacterial strains Ruminobacter amylophilus, Fibrobacter succinogenes and Enterococcus faecium for growth promotion in maize and soybean plants

2020 ◽  
pp. 2020-2027
Author(s):  
Lívia de Paula Silveira Mello ◽  
Ana Cláudia dos Santos ◽  
Roberta Mendes dos Santos ◽  
Saveetha Kandasamy ◽  
George Lazarovits ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Development ◽  
Enterococcus Faecium ◽  
Growth Promotion ◽  
Production Costs ◽  
Phosphorus Concentration ◽  
Fibrobacter Succinogenes ◽  
Plant Growth Promoting Bacteria ◽  
Biomass Carbon ◽  
Soil Biomass

Ruminobacter amylophilus, Fibrobacter succinogenes and Enterococcus faecium have characteristics that are similar to those of plant growth-promoting bacteria and can be used to promote plant development and reduce production costs. These bacteria were isolated from fistulated ruminants and are gram-negative, anaerobic or facultative anaerobic. These bacteria are frequently used to increase animal productivity through the production of many enzymes responsible for the carbon cycle and the release of other nutrients by organic matter decomposition. The bacteria R. amylophilus, F. succinogenes and E. faecium have growth promotion abilities, such as phosphorus solubilization, nitrogen promotion, and indole acetic acid and siderophore production. Tests were performed under greenhouse conditions with soybean and maize crops with five treatments and six replications. The first treatment was the control (without inoculant); the other treatments included each species of bacteria, and there was a treatment with a mixture (mix) of the three bacteria. F. succinogenes increased the root dry mass of maize by 21.4%, as well as the nitrogen and phosphorus contents, compared to the control. R. amylophilus and E. faecium decreased the phosphorus concentration in shoots of maize, and R. amylophilus increased the soil biomass carbon by 76.39% compared to the mix under maize cultivation, while E. faecium decreased the soil biomass carbon by 56.78% compared to the mix under soybean cultivation. The present study verified that Ruminobacter amylophilus, Fibrobacter succinogenes and Enterococcus faecium presented plant growth-related abilities and could be used to improve plant development, reducing the necessity of chemical fertilizers

scholarly journals Editorial: Identifying agri-food research priorities for Spain - 2017 results

2018 ◽  
Vol 16 (3) ◽  
pp. e0001 ◽  
Author(s):  
Marta García ◽  
Ángeles Alonso ◽  
María Luisa Tello ◽  
Marta De la Poza ◽  
Natalia Villalobos ◽  
...  
Keyword(s):  
Value Chain ◽  
Environmental Sustainability ◽  
Rural Areas ◽  
Production Systems ◽  
New Technology ◽  
Research Priorities ◽  
Production Costs ◽  
Chain Process ◽  
Food Research ◽  
Forestry Production

Among other functions, the INIA is involved in national and international cooperation in the field of agri-food research. The process of identifying and classifying gaps in our knowledge forms an essential part of this effort. This article describes that process, the tools and the materials used to achieve the final objective, namely, the identification of research priorities in the Spanish agri-food sector in order to deal with the societal challenges posed by society and the stakeholders involved. These challenges, within the context of the bioeconomy, are the sustainability of primary and forestry production systems, the safety and quality of food and bioproducts as well as the competitiveness of farmers and companies in this sector. It is necessary to optimize resource management and means of production along with improved efficiency to guarantee sustainability throughout the value chain process. The main goal, under the current scenario of climate change, is to develop models which lead to a balance between food quality and production costs (competitiveness and economic sustainability), ecosystem conservation and mitigation of the environmental impacts (environmental sustainability) while maintaining the population in rural areas (social sustainability). These models will be based on new technology in both intensive and extensive production systems. They should support the improvement and valuation of traditional products together with the formulation and development of foods with new functionalities and quality while at the same time ensuring safety. As well as satisfying consumer demand, improved knowledge must lead to a more efficient use of our own resources and by-products within the framework of a circular economy, including the development of bioproducts, eco-innovation and eco-design.

Sign in / Sign up

Export Citation Format

Share Document