Effect of Dietary Tryptophan on Growth, Intestinal Microbiota, and Intestinal Gene Expression in an Improved Triploid Crucian Carp

Tryptophan (Trp) has received increasing attention in the maintenance of intestinal function. In this study, improved triploid crucian carp (ITCC) fed diets containing 6.35 g kg−1 Trp had higher average daily gain (ADG) and improved villus height (VH) and crypt depth (CD) in the intestine compared to the control group. To elucidate the potential mechanisms, we used RNA sequencing (RNA-seq) to investigate changes in the intestinal transcriptome and 16S rRNA gene sequencing to measure the intestinal microbiota in response to 6.35 g kg−1 Trp feeding in ITCC. Dietary Trp altered intestinal gene expression involved in nutrient transport and metabolism. Differentially expressed transcripts (DETs) were highly enriched in key pathways containing protein digestion and absorption and the AMPK signaling pathway. 16S rRNA sequencing showed that 6.35 g kg−1 Trp significantly increased the abundance of the genus Cetobacterium, and the Firmicutes/Bacteroidetes ratio at the phylum level (P < 0.05). In addition, bacterial richness indices (Simpson index) significantly increased (P < 0.05) community evenness in response to 6.35 g kg−1 Trp. In conclusion, appropriate dietary Trp improves the growth performance, and influences the intestinal flora of ITCC. This study might be helpful to guide the supply of dietary exogenous Trp in ITCC breeding.

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Effect of Massa Medicata Fermentata on the Gut Microbiota of Dyspepsia Mice Based on 16S rRNA Technique

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/7643528 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Xiaorui Zhang ◽

Hongling Zhang ◽

Qinwan Huang ◽

Jilin Sun ◽

Renchuan Yao ◽

...

Keyword(s):

16S Rrna ◽

Intestinal Microflora ◽

Intestinal Flora ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

Blank Group ◽

Intestinal Microorganisms ◽

Specific Pathogen ◽

Rrna Gene Sequencing ◽

Aseptic Conditions

Massa Medicata Fermentata (MMF) is a traditional Chinese medicine (TCM) for treating indigestion and its related disorders. This study analyzes the effect of MMF on intestinal microorganisms in dyspepsia mice based on 16S rRNA technology. We take a dyspepsia model caused by a high-protein, high-calorie, high-fat diet. The 60 specific-pathogen free Kunming (SPF KM) mice were randomly divided into a model group n=12, an MMF group (LSQ group, n=12), a Jianweixiaoshi group (JWXS group, n=12), a domperidone group (DP group, n=12), and a blank group n=12. On the seventh day of administration, mice were fasted and deprived of water. After 24 h, take the second feces of stress defecation in mice under strict aseptic conditions and quickly transfer them to a sterile cryotube. This study comprehensively evaluates the α-diversity, β-diversity, flora abundance and composition of each group of miceʼs intestinal microorganisms, and their correlation with functional dyspepsia based on the 16S rRNA gene sequencing technology. After modeling, some dyspepsia reactions, proximal gastric relaxation reduction, and intestinal microflora changes were noted. Dyspepsia mice showed dyspepsia reactions and proximal gastric relaxation reduction, characterized by a significant decrease of contents of gastrin P<0.01 and cholinesterase P<0.01. MMF can improve dyspepsia symptoms and promote proximal gastric relaxation. Significant intestinal flora disorders were found in dyspepsia mice, including downregulation of Bacteroidetes, Lactobacillus, and Prevotellaceae and upregulation of Proteobacteria, Verrucomicrobia, Epsilonbacteraeota, Firmicutes, Lachnospiraceae NK4A136 group, and Lachnospiraceae. MMF could alleviate intestinal microflora disturbance, and the regulation effect of MMF on Bacteroidetes, Verrucomicrobia, and Epsilonbacteraeota was more reliable than that of Jianweixiaoshi tables and domperidone. The intestinal microflora may be correlated with the promoted digestion of MMF.

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Microbial diversity and activity in the Nematostella vectensis holobiont: insights from 16S rRNA gene sequencing, isolate genomes, and a pilot-scale survey of gene expression

Frontiers in Microbiology ◽

10.3389/fmicb.2015.00818 ◽

2015 ◽

Vol 6 ◽

Cited By ~ 12

Author(s):

Jia Y. Har ◽

Tim Helbig ◽

Ju H. Lim ◽

Samodha C. Fernando ◽

Adam M. Reitzel ◽

...

Keyword(s):

Gene Expression ◽

16S Rrna ◽

16S Rrna Gene ◽

Microbial Diversity ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Pilot Scale ◽

Nematostella Vectensis ◽

Rrna Gene ◽

Rrna Gene Sequencing

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High-throughput 16S rRNA gene sequencing reveals alterations of mouse intestinal microbiota after radiotherapy

Anaerobe ◽

10.1016/j.anaerobe.2015.01.004 ◽

2015 ◽

Vol 33 ◽

pp. 1-7 ◽

Cited By ~ 40

Author(s):

Young Suk Kim ◽

Jinu Kim ◽

Soo-Je Park

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Intestinal Microbiota ◽

High Throughput ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

Rrna Gene Sequencing

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The Effect of Xuebijing on the Gut Microbiota and Metabolic of Heat 1 Stroke Rat 2

10.21203/rs.3.rs-142370/v1 ◽

2021 ◽

Author(s):

Qiang wen ◽

Xuan He ◽

Yu Shao ◽

Lun Peng ◽

Li Zhao ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Gut Microbiome ◽

Heat Stroke ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Control Group ◽

Rrna Gene ◽

Metabolism Pathway ◽

Rrna Gene Sequencing

Abstract The goal of the present study was to evaluate the fecal microbiome and serum metabolites in 16 Xuebijing (XBJ)-injected rats after heat stroke using 16S rRNA gene sequencing and gas chromatography-mass spectrometry (GC-MS) metabolomics. Eighteen rats were divided into the control group (CON), heat stroke group (HS), and XBJ group. The 16S rRNA gene sequencing results revealed that the abundance of Bacteroidetes was overrepresented in the XBJ group compared to the HS group, while Actinobacteria was underrepresented. Metabolomic profiling showed that the pyrimidine metabolism pathway, pentose phosphate pathway, and glycerophospholipid metabolism pathway were upregulated in the XBJ group compared to the HS group. Taken together, these results demonstrated that heat stroke not only altered the gut microbiome community structure of rats but also greatly affected metabolic functions, leading to gut microbiome toxicity.

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Identification microflora related to growth performance in pigs based on 16s rRNA sequence analyses

10.21203/rs.3.rs-62183/v1 ◽

2020 ◽

Author(s):

Xin-Jian Li ◽

Mingyu Wang ◽

Yahui Xue ◽

Dongdong Duan ◽

Cong Li ◽

...

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

Growth Performance ◽

Intestinal Epithelial Cells ◽

Average Daily Gain ◽

Intestinal Flora ◽

Rrna Gene ◽

Intestinal Epithelial ◽

Microbial Composition ◽

Mc4r Gene

Abstract Intestinal microorganisms have been shown to be important factors that affect the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n = 10), Landrace (n = 9) and Yorkshire (n = 21) pigs under the same diet and feeding conditions were collected. The fecal microbial composition was profiled via 16S ribosomal RNA (rRNA) gene sequencing. And we also analyzed their growth performance. We found that Duroc and Landrace had significant differences in average daily gain (ADG), feed efficiency ratio (FER), growth index (GI), and 100 kg daily age (P < 0.05). Moreover, through the analysis of the intestinal flora, we also identified that there were 18 species of intestinal flora with significant differences between Duroc and Landrace (P < 0.05). In order to eliminate the influence of genetic background, the intestinal differential flora of 21 Yorkshire pigs with different growth performance was analyzed. The results showed that there were significant correlations between Barnesiella, Dorea, Clostridium and Lactobacillus and pig growth performance. To explore the effect of intestinal flora on the growth performance of pigs at the molecular level, Lactobacillus, which has the highest content in the intestine, was selected for isolation and purification, and co-cultured with intestinal epithelial cells. The qPCR was used to determine the effect of Lactobacillus on MC4R gene expression in intestinal epithelial cells. The results showed that Lactobacillus inhibited MC4R gene expression in intestinal epithelial cells. The results provided useful reference for the further study on the relationship between intestinal flora and pig growth performance.

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Epidemiological, biochemical and antimicrobial susceptibility characteristics of Streptococcus pseudoporcinus isolated in Quebec, Canada, from 1997 to 2006

Journal of Medical Microbiology ◽

10.1099/jmm.0.47295-0 ◽

2007 ◽

Vol 56 (12) ◽

pp. 1620-1624 ◽

Cited By ~ 16

Author(s):

Christiane Gaudreau ◽

Esther Simoneau ◽

Olivia Labrecque ◽

Robert A. Laurence ◽

Céline Laferrière ◽

...

Keyword(s):

16S Rrna ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Sub Saharan Africa ◽

Control Group ◽

Rrna Gene ◽

The Caribbean ◽

Sub Saharan ◽

Rrna Gene Sequencing ◽

Animal Isolates

From 1997 to 2006, in the province of Quebec, Canada, 15 isolates of Streptococcus pseudoporcinus from 1 urine and 14 vaginorectal cultures were recovered from the genitourinary tract of pregnant women. All these women originated from the Caribbean or sub-Saharan Africa (P=0.00045 compared with a suitable control group). The S. pseudoporcinus isolates were compared to eight isolates of Streptococcus porcinus identified in Quebec from 1995 to 2006, all from animals, of which five were swine. 16S rRNA gene sequencing was required to differentiate between S. pseudoporcinus and S. porcinus animal isolates.

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A Correlation Study between Clinical Efficacy of Weifuchun on Precancerous Lesions of Gastric Cancer and Intestinal flora

10.21203/rs.3.rs-571877/v1 ◽

2021 ◽

Author(s):

Yanqin Bian ◽

Xi Chen ◽

Hongyan Cao ◽

Dong Xie ◽

Meiping Zhu ◽

...

Keyword(s):

Gastric Cancer ◽

16S Rrna ◽

16S Rrna Gene ◽

Intestinal Microbiota ◽

Roc Analysis ◽

Precancerous Lesions ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

Rrna Gene Sequencing

Abstract BackgroudWeifuchun(WFC), a Chinese herbal prescription consisting of Red Ginseng, Isodon amethystoides and Fructus Aurantii, is commonly used in China to treat a variety of chronic stomach disorders. The aim of the paper is to determine the effect of WFC on intestinal microbiota changes in precancerous lesions of gastric cancer (PLGC) patients. MethodsPLGC patients of H.pylori negative were randomly divided into two groups received either WFC tablets at a dose of 1.44g 3 times a day or vitacoenzyme tablets at a dose of 0.8g 3 times a day. All patients were treated for 6 months, consecutively. Gastroscopy and histopathology were used to assess the histopathological changes in the gastric tissues before and after treatment. 16S rRNA gene sequencing was carried out to assess the effects WFC on intestinal microbiota changes in PLGC patients. Receiver Operating Characteristics(ROC) analysis was used to assess the sensitivity and specificity of different intestinal microbiota in distinguishing between PLGC patients and healthy control. ResultsGastroscopy and histopathological results indicated that WFC could improve the pathological condition of PLGC patients, especially in the atrophy or intestinal metaplasia. The results of 16S rRNA gene sequencing indicated that WFC could regulate the microbial diversity, microbial composition, and abundance of the intestinal microbiota of PLGC patients. Following WFC treatment, the relative abundance of Parabacteroides was decreased in WFC group when compared with the control group. ROC analysis found that the Parabacteroides could effectively distinguish PLGC patients from healthy individuals with sensitivity of 0.79 and specificity of 0.8. ConclusionsWFC could slow down the progression of PLGC by regulating intestinal microbiota abundance. Trial registrationNCT03814629. Name of registry: Randomized Clinical Trial: Weifuchun Treatment on Precancerous Lesions of Gastric Cancer. Registered 3 August 2018-Retrospectively registered, https://register.clinicaltrials.gov/ NCT03814629.

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