scholarly journals Evaluation of Serum miR-17-92 Cluster as Noninvasive Biomarkers for Bladder Cancer Diagnosis

2021 ◽  
Vol 11 ◽  
Author(s):  
Jingyao Wang ◽  
Xiqi Peng ◽  
Rongkang Li ◽  
Kaihao Liu ◽  
Chunduo Zhang ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Diagnosis ◽  
Clinical Stage ◽  
Diagnostic Model ◽  
Qrt Pcr ◽  
Noninvasive Biomarker ◽  
Noninvasive Biomarkers ◽  
Cancer Tissues ◽  
Geo Database

Previous studies have shown that the miR-17-92 cluster is involved in the occurrence and development of bladder cancer. However, the role of serum miR-17-92 cluster in the diagnosis of bladder cancer has not been studied. In the present study, we evaluated the expression of miR-17-92 cluster members in bladder cancer tissues by analyzing 428 cases from TCGA database. Next, we collected the sera of 74 bladder cancer patients and 90 controls, and used qRT-PCR to detect the relative expression of the cluster. The results showed that the expression of the cluster members in the sera of patients were significantly higher than that of the controls, and they were positively correlated with the clinical stage and pathological grade of the patients. We evaluated their ability to diagnose bladder cancer using ROC, of which miR-92a-3p (AUC = 0.902), miR-17-5p (AUC = 0.845) and miR-20a-5p (AUC = 0.806) were the most prominent. Finally, we established a diagnostic model by logistic regression (AUC = 0.969). We further validated the results of the study using another dataset from the GEO database. Moreover, we evaluated the prognostic value of the cluster. The results revealed that miR-20a-5p was correlated with recurrence of bladder cancer. In summary, the present study validated the overexpression of serum miR-17-92 cluster in bladder cancer. The model composed of the three cluster members were confirmed to be a promising noninvasive biomarker for bladder cancer diagnosis.

MicroRNAs as Noninvasive Biomarkers in Bladder Cancer Detection: A Diagnostic Meta-Analysis Based on qRT-PCR Data

2016 ◽  
Vol 31 (3) ◽  
pp. 276-285 ◽  
Author(s):  
Shuhui Xiao ◽  
Jinfeng Wang ◽  
Ning Xiao
Keyword(s):  
Bladder Cancer ◽  
Cancer Detection ◽  
Cancer Diagnosis ◽  
Meta Analysis ◽  
Diagnostic Value ◽  
Diagnostic Efficiency ◽  
Diagnostic Significance ◽  
Qrt Pcr ◽  
Meta Regression ◽  
Noninvasive Biomarkers

Objective As the diagnostic significance of microRNAs (miRNAs) in the detection of bladder cancer is controversial, we aimed to perform a meta-analysis to comprehensively assess the diagnostic value of miRNAs in blood and urine for detecting bladder cancer. Methods A systematic literature search of public databases was conducted to obtain qualified studies. Sensitivity was utilized to plot the summary receiver operator characteristic (SROC) curve against specificity and the area under the SROC curve (AUC) was generated to evaluate the pooled diagnostic efficiency. Subgroup analyses and meta-regression were applied to investigate the underlying sources of heterogeneity. The STATA 12.0 software was used to perform all statistic analyses. Results A total of 58 studies from 22 articles comprising 4,558 bladder cancer patients and 4,456 controls were included in our meta-analysis. MiRNAs in blood and urine manifested relatively good diagnostic efficiency in detecting bladder cancer, with a sensitivity of 0.74, a specificity of 0.78, and an AUC of 0.83. Multiple-miRNA assays were more accurate than single-miRNA ones in bladder cancer diagnosis. Blood-based miRNA assays displayed better diagnostic performance than urine-based ones. In addition, miRNAs showed reduced diagnostic value in bladder cancer among Caucasians compared with Asians. Conclusions MiRNAs in blood and urine, especially the combination of multiple miRNAs, may serve as hopeful noninvasive biomarkers for early diagnosis of bladder cancer. Further extensive prospective research is needed to verify their clinical significance in bladder cancer diagnosis.

Bladder Cancer Diagnosis: The Role of CT Urography

2015 ◽  
Vol 101 (4) ◽  
pp. 412-417 ◽  
Author(s):  
Emanuela Capalbo ◽  
Anna Kluzer ◽  
Michela Peli ◽  
Maria Cosentino ◽  
Elisabetta Berti ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Diagnosis ◽  
Ct Urography

scholarly journals Lnc BACE1-AS Promotes The Progression of Osteosarcoma Through miR-762/SOX7 Axis

2021 ◽  
Author(s):  
qu yanlong ◽  
Wang Chunlei ◽  
Zhang Tao ◽  
Yang Li ◽  
Na Xinyu
Keyword(s):  
Cell Proliferation ◽  
Binding Sites ◽  
Molecular Basis ◽  
Cell Counting ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Qrt Pcr ◽  
Lower Expression ◽  
Geo Database

Abstract Background: Osteosarcoma (OS) is a rare malignant primary tumor of mesenchymal origin affecting bone that occurs in adolescents and children. LncRNAs are important regulators of tumorigenesis and development. This study aimed to explore the role and molecular basis of LncRNA BACE1-AS in OS. Methods and results : Through the analysis of differential expressed lncRNAs in OS tissues by GEO database, LncRNA BACE1-AS displayed a remarkably lower expression. This found could also be observed in both OS tissues and cell lines by qRT-PCR. Furthermore, using Cell counting kit-8 (CCK-8), transwell, wound healing and westernblot assays, overexpression LncRNA BACE1-AS remarkably reduced cell proliferation, migration and invasion abilities in OS. In addition, LncRNA BACE1-AS was validated as a sponge of miR-762 through the prediction of lncRNASNP. Further, luciferase reporter and RIP assays were conducted to confirm the binding sites between LncRNA BACE1-AS and miR-762. SOX7 was a target of miR-762 and could be regulate by LncRNA BACE1-AS. Moreover, inhibition of miR-762 could attenuate the role of sh-LncRNA BACE1-AS in OS cells, at meanwhile reduced the expression of SOX7. Conclusion : In this study, LncRNA BACE1-AS regulated proliferation, migration, invasion and apoptosis of OS cells by miR-762/SOX7 axis, implying that LncRNA BACE1-AS was a potential target for OS therapy.

Expression of Beclin 1 in Bladder Cancer and Its Clinical Significance

2013 ◽  
Vol 28 (1) ◽  
pp. 56-62 ◽  
Author(s):  
Gui-hong Liu ◽  
Qian Zhong ◽  
Yun-lin Ye ◽  
Hong-bo Wang ◽  
Li-juan Hu ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Histological Grade ◽  
Quantitative Polymerase Chain Reaction ◽  
Clinical Stage ◽  
Mrna Level ◽  
Prognostic Significance ◽  
Beclin 1 ◽  
Normal Bladder ◽  
Muscle Invasive ◽  
Cancer Tissues

Background The aim of this study is to explore the expression of beclin 1, an autophagy gene, in bladder cancer and to evaluate its clinical and prognostic significance in patients with bladder cancer. Methods Beclin 1 expression was examined at mRNA and protein levels by real-time quantitative polymerase chain reaction (RT-PCR), Western blotting, and immunohistochemistry in bladder cancer tissues and adjacent normal bladder tissues. The relationship between the expression of beclin 1 and clinicopathological characteristics and prognosis was statistically analyzed. Results mRNA level, protein expression and immunoreactivity of beclin 1 were decreased in bladder cancer tissues compared with adjacent normal tissues. Downregulation of beclin 1 was more frequent in tumors with higher histological grades (the expression of beclin 1 was reduced by 49.0% in G1 and G2, and by 71.8% in G3, p=0.010), and was also reduced by 69.5% in the muscle invasive type and by 51.1% in the non-muscle invasive type (p=0.04). Reduced beclin 1 expression was positively associated with higher histological grade and more advanced clinical stage (p<0.05). Kaplan-Meier survival analysis revealed that patients exhibiting lower beclin 1 expression experienced a shorter survival than those with higher expression (p=0.006). Cox proportional hazards regression analysis showed that beclin 1 protein is an independent predictor of survival (p=0.005). Conclusion Beclin 1 has an influence on the progression of bladder cancer and might serve as a potential prognostic factor for patients with bladder cancer.

scholarly journals Expression of caveolin-2 in patients with oral cancer and correlations with clinicopathological parameters

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 703-716
Author(s):  
Chao Wan ◽  
Fang Zhang ◽  
Liangming Zhu
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Clinical Stage ◽  
Qrt Pcr ◽  
Human Protein Atlas ◽  
Pathological Differentiation ◽  
Oral Cancer Patients ◽  
Differentiation Degree ◽  
Tissue Specimens ◽  
Cancer Tissues

We aimed to analyze the expression of caveolin-2 (CAV2) in patients with oral cancer and its correlations with clinicopathological parameters.The expression of CAV2 in oral cancer and its influence on the survival curves of oral cancer patients were inquired through the Human Protein Atlas Database. The cancer tissue specimens and normal paracancerous tissue specimens (?2 cm away from cancer tissues) were collected from 173 patients with oral cancer confirmed by pathology. Moreover, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry were performed to detect the messenger ribonucleic acid (mRNA) and protein expressions of CAV2 in oral cancer tissues and corresponding paracancerous tissues, respectively, and their associations with the clinicopathological characteristics and survival conditions of oral cancer patients were analyzed.It was shown in the Human Protein Atlas Database that the expression of CAV2 was increased significantly in oral cancer tissues compared with that in normal tissues (P<0.05), and patients with a low expression of CAV2 had a longer survival time than those with a high expression of CAV2 (P<0.05). The results of qRT-PCR and immunohistochemistry manifested that the mRNA expression level of CAV2 and the percentage of CAV2-positive cells were significantly higher in oral cancer tissues than those in paracancerous tissues (P<0.05). The CAV2 expression was correlated with clinical stage and pathological differentiation degree (P<0.05). In comparison with those in patients with a low CAV2 expression, the overall survival (OS) curve, relapse-free survival (RFS) curve and survival rate declined significantly in patients with a high CAV2 expression (P=0.001). Besides, the CAV2 expression, clinical stage and pathological differentiation degree were independent influencing factors for the postoperative OS and RFS of patients. The expression of CAV2 had relatively high predictive value for the OS and RFS of patients with oral cancer within 5 years after operation, of which the area under curve was 0.827 and 0.874, and the optimal cut-off value was 27.97% and 32.84%, respectively.CAV2 is highly expressed in oral cancer. With rising CAV2 expression level, the survival time of patients is shortened and the relapse risk is elevated, suggesting a poor prognosis.

scholarly journals EGF-induced nuclear translocation of SHCBP1 promotes bladder cancer progression through inhibiting RACGAP1-mediated RAC1 inactivation

2022 ◽  
Vol 13 (1) ◽  
Author(s):  
Hubin Yin ◽  
Chen Zhang ◽  
Zongjie Wei ◽  
Weiyang He ◽  
Ning Xu ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cell Migration ◽  
Cancer Progression ◽  
Nuclear Translocation ◽  
Cell Movement ◽  
Egfr Activation ◽  
Bladder Cancer Cells ◽  
Cancer Tissues ◽  
Multiple Signaling

AbstractBladder cancer is a highly heterogeneous and aggressive malignancy with a poor prognosis. EGF/EGFR activation causes the detachment of SHC-binding protein 1 (SHCBP1) from SHC adapter protein 1 (SHC1), which subsequently translocates into the nucleus and promotes cancer development via multiple signaling pathways. However, the role of the EGF-SHCBP1 axis in bladder cancer progression remains unexplored. Herein, we report that SHCBP1 is upregulated in bladder cancer tissues and cells, with cytoplasmic or nuclear localization. Released SHCBP1 responds to EGF stimulation by translocating into the nucleus following Ser273 phosphorylation. Depletion of SHCBP1 reduces EGF-induced cell migration and invasiveness of bladder cancer cells. Mechanistically, SHCBP1 binds to RACGAP1 via its N-terminal domain of amino acids 1 ~ 428, and this interaction is enhanced following EGF treatment. Furthermore, SHCBP1 facilitates cell migration by inhibiting RACGAP-mediated GTP-RAC1 inactivation, whose activity is indispensable for cell movement. Collectively, we demonstrate that the EGF-SHCBP1-RACGAP1-RAC1 axis acts as a novel regulatory mechanism of bladder cancer progression, which offers a new clinical therapeutic strategy to combat bladder cancer.

scholarly journals LINC00958 promotes bladder cancer carcinogenesis by targeting miR-490-3p and AURKA

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hongtao Zhen ◽  
Peng Du ◽  
Qiang Yi ◽  
Xiaolong Tang ◽  
Tongqing Wang
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Cancer Progression ◽  
Treatment Method ◽  
Bladder Cancer Cells ◽  
Cell Progression ◽  
New Treatment ◽  
Cancer Tissues ◽  
Proliferation And Invasion

Abstract Background Bladder cancer is a prevalent malignancy of the urinary system, in which long non-coding RNAs (lncRNAs) are highly associated. We aimed to elucidate the role of LINC00958 in bladder cancer. Methods LINC00958 expression levels were measured using qRT-PCR. The interaction of LINC00958-miR-490-3p-AURKA was analyzed by luciferase, RIP, and RNA pull-down assays. The biological roles of LINC00958, miR-490-3p, and AURKA in bladder cancer cells were analyzed using CCK8, BrdU, and transwell assays. Results Increased expression of LINC00958 and AURKA was observed in bladder cancer tissues and cell lines. Decreased LINC00958 expression repressed bladder cancer progression and downregulation of miR-490-3p accelerated bladder cancer cell progression. Moreover, LINC00958 sponges miR-490-3p to upregulate AURKA expression, thereby promoting carcinogenesis in bladder cancer cells. Conclusions Our study revealed that LINC00958 facilitated cell proliferation and invasion, and suppressed cell apoptosis by sponging miR-490-3p and upregulating AURKA, thus inspiring a new treatment method for bladder cancer.

Evaluation of Urine NDRG1 as Noninvasive Biomarker for Bladder Cancer Diagnosis

2021 ◽  
Vol 67 (03/2021) ◽  
Author(s):  
Yu-Jing Sun ◽  
Ai-Wei Li ◽  
Yun-Cong Zhang ◽  
Ju-Hua Dai ◽  
Xi Zhu
Keyword(s):  
Bladder Cancer ◽  
Cancer Diagnosis ◽  
Noninvasive Biomarker

scholarly journals Long Noncoding RNA UCA1 Is Related to Autophagy and Apoptosis in Endometrial Stromal Cells

2021 ◽  
Vol 10 ◽  
Author(s):  
Lili Jiang ◽  
Yahui Wan ◽  
Ziyi Feng ◽  
Da Liu ◽  
Ling Ouyang ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Research Question ◽  
Pcr Analysis ◽  
Endometrial Stromal Cells ◽  
Qrt Pcr ◽  
Cancer Tissues

Research QuestionThe expression of the long noncoding RNA (lncRNA) urothelial carcinoma-associated 1 (UCA1) in embryonic tissues is higher than that in most cancer tissues, such as bladder cancer, indicating that RNA is a carcinoembryonic antigen. However, there are no published reports on the role of UCA1 in endometriosis (EMS). Therefore, to address this gap in knowledge, we assessed the potential role of lncRNA UCA1 in the pathogenesis and progression of EMS.DesignTo verify the expression of UCA1 in EMS, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used. RNA interference (siRNA) was used to study the biological function of UCA1 in EMS in vitro.ResultsqRT-PCR analysis showed that the expression of lncRNA UCA1 in EMS was increased (P&lt;0.01). Knockdown of UCA1 in vitro significantly inhibited the proliferation of endometrial stromal cells (ESCs) and induced autophagy and apoptosis.ConclusionUCA1 is highly expressed in EMS and promotes the proliferation of ESCs but suppresses autophagy and apoptosis. In EMS, UCA1 may be a prognostic marker and therapeutic target.

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