Abnormalities of Serum Fatty Acids in Children With Henoch–Schönlein Purpura by GC-MS Analysis

Purpose: The objectives of this work were to test the levels of serum medium- and long- chain fatty acids (MLCFAs) in children and to discover their possible relationship with Henoch-Schönlein Purpura (HSP), also known as Immunoglobulin A vasculitis.Methods: A total of 57 children with HSP (HSP group) and 28 healthy children (CON group) were recruited for this study. Serum specimens were collected to detect the compositions and contents of MLCFAs by gas chromatography with mass spectrometry (GC-MS) analysis.Results: The contents of all detected 37 MLCFAs in the HSP group were higher than the healthy group. Thirty-one species of MLCFAs were discovered to have a significant difference (p < 0.05) in two groups. Comparing to healthy controls, there were 31, 31, 18 fatty acids showed a statistical difference in the untreated group, regular treated group, and withdrawal group of HSP, respectively. The trend of fatty acids in the three HSP groups was similar to the healthy controls, as well as the untreated group and regular treated group changed more obviously than the withdrawal group. Almitate (C16:0) and 18 carbon atoms (C18) of fatty acids were abundant in all three HSP groups, divided according to the treatment of glucocorticoid. Some fatty acids were found having considerable differences (p < 0.05) in three groups. Monounsaturated fatty acids (MUFAs), including elaidate (C18:1T), cis-11,14,17-eicosatrienoic acid ester (C20:1), and cis-15-tetracosenoate (C24:1), were distinctly higher in HSP children with renal damage.Conclusion: Our study revealed that the abnormalities in MLCFA may be associated with the development of HSP. Another interesting finding was that fatty acids contents were changing during the glucocorticoid treatment. Meanwhile, long-chain MUFAs may have an impact on renal damage in HSP patients. Further studies need to be carried out in order to explore the specific mechanism of fatty acids in the course of HSP.

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Role of Serum Fatty Acids in Children with Henoch-Schönlein purpura by GC-MS analysis

10.21203/rs.3.rs-16534/v1 ◽

2020 ◽

Author(s):

Min Wen ◽

shipin feng ◽

xiqiang dang ◽

xuewei ding ◽

zhiquan xu ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Fatty Acid Metabolism ◽

Renal Damage ◽

Acid Metabolism ◽

Henoch Schonlein Purpura ◽

Schonlein Purpura ◽

Henoch Schönlein Purpura ◽

Long Chain ◽

Serum Fatty Acids

Abstract BACKGROUND: The objectives of this work were to discover the changes of serum Medium- and Long-Chain fatty acids levels and its possible relationship with Henoch-Schönlein Purpura (HSP), also referred to as Immunoglobulin A vasculitis in children. METHODS: A total of 58 children with HSP and 28 healthy children were recruited for this study. Serum fatty acids were analyzed by gas chromatography with mass spectrometry (GC-MS). RESULTS: 31 species of Fatty acids were discovered to have a significant difference between HSP group and healthy control group (CON group). The contents of all detected 37 fatty acids in the HSP group were higher than the healthy group. Parts of fatty acids were found in our study having significant change according to the treatment. Palmitate (C16:0) and 18 carbon atoms (C18) of fatty acids were abundant in all three groups of HSP. Elaidate (C18:1T), cis-11,14,17-Eicosatrienoic acid ester (C20:1) and cis-15-tetracosenoate (C24:1) were found to have a correlation on renal damage of HSP. CONCLUSION: Our study provides clinical evidence to support that fatty acid metabolism is associated with HSP by GC-MS method. Glucocorticoid therapy has a certain relationship with fatty acid metabolism during HSP treatment. Meanwhile, long-chain MUFAs may have an impact on renal damage of HSP. In addition, we speculate that a low BMI may be a kind of manifestation of abnormal fatty acid metabolism in HSP. All in all, further study is needed to explore the specific mechanism of fatty acids and HSP.

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Beneficial effects of creatine phosphate sodium for the treatment of Henoch–Schönlein purpura in patients with early renal damage detected using urinary kidney injury molecule-1 levels

European Journal of Pediatrics ◽

10.1007/s00431-015-2601-x ◽

2015 ◽

Vol 175 (1) ◽

pp. 49-55 ◽

Cited By ~ 2

Author(s):

Jianjiang Zhang ◽

Huiqin Zeng ◽

Na Wang ◽

Xiyan Tian ◽

Wenjie Dou ◽

...

Keyword(s):

Renal Damage ◽

Kidney Injury ◽

Creatine Phosphate ◽

Henoch Schonlein Purpura ◽

Beneficial Effects ◽

Kidney Injury Molecule 1 ◽

Schonlein Purpura ◽

Henoch Schönlein Purpura

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Intestinal dysbiosis featuring abundance of Streptococcus associates with Henoch-Schönlein purpura nephritis (IgA vasculitis with nephritis) in adult

BMC Nephrology ◽

10.1186/s12882-021-02638-x ◽

2022 ◽

Vol 23 (1) ◽

Author(s):

Jiaxing Tan ◽

Zhengxia Zhong ◽

Yi Tang ◽

Wei Qin

Keyword(s):

Gut Microbiota ◽

Intestinal Microbiota ◽

Fecal Microbiota ◽

Healthy Controls ◽

Henoch Schonlein Purpura ◽

Intestinal Dysbiosis ◽

Clinical Indices ◽

Schonlein Purpura ◽

Henoch Schönlein Purpura ◽

Purpura Nephritis

Abstract Background The pathogenesis of Henoch-Schönlein purpura nephritis (HSPN) is closely associated with mucosal infection. But whether intestinal microbiota dysbiosis plays a role in it is not clear. Methods A total of 52 participants including 26 HSPN patients and 26 healthy controls were included. By using 16S ribosomal RNA gene sequencing, the intestinal microbiota composition between HSPN and healthy controls was compared. The diagnostic potency was evaluated by Receiver operating characteristic (ROC) with area under curves (AUC). Meanwhile, correlation analysis was also performed. Results The lower community richness and diversity of fecal microbiota was displayed in HSPN patients and the structure of gut microbiota was remarkedly different. A genus-level comparison indicated a significant increase in the proportions of g-Bacteroides, g-Escherichia–Shigella and g-Streptococcus, and a marked reduction of g-Prevotella_9 in HSPN patients, suggesting that the overrepresentation of potential pathogens and reduction of profitable strains were the main feature of the dysbiosis. The differential taxonomic abundance might make sense for distinguishing HSPN from healthy controls, with AUC of 0.86. The relative abundance of the differential bacteria was also concerned with clinical indices. Among them, Streptococcus spp. was positively associated with the severity of HSPN (P < 0.050). It was found that HSPN patients with higher level of Streptococcus spp. were more likely to suffering from hematuria and hypoalbuminemia (P < 0.050). Conclusions The dysbiosis of gut microbiota was obvious in HSPN patients, and the intestinal mucosal streptococcal infection was distinctive, which was closely related to its severity.

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Clinical Characteristics and Risk Factors of Gastrointestinal Perforation in Children with Henoch-Schönlein Purpura

10.21203/rs.3.rs-37348/v1 ◽

2020 ◽

Author(s):

Qingyin Guo ◽

Xiaolei Hu ◽

Chundong Song ◽

Xianqing Ren ◽

Wensheng Zhai ◽

...

Keyword(s):

Risk Factors ◽

Abdominal Pain ◽

Intestinal Perforation ◽

Renal Damage ◽

Gastrointestinal Perforation ◽

Small Vessel Vasculitis ◽

Control Group ◽

Henoch Schonlein Purpura ◽

Schonlein Purpura ◽

Henoch Schönlein Purpura

Abstract Background: Henoch-Schönlein purpura (HSP) is a common small vessel vasculitis in children. Gastrointestinal perforation (GP) rarely presents as a complication of HSP and was not well characterized. This study aimed to investigate the clinical features, diagnosis and risk factors of GP in children with HSP. Methods: We retrospectively reviewed the clinical data of 10791 children with HSP who attended our hospital between January 2014 and June 2018 and analyzed the treatment and clinical risk factors of 11 children with HSP complication with GP. Results: GP occurred in 11 children with HSP, with an incidence of 0.10%. Among the 11 cases HSP with GP, 1 case was gastric perforation and 10 cases were intestinal perforation. CT indicates perforation but ultrasonography did not indicate perforation in 5 cases of GP patients. The average duration of abdominal pain in HSP with GP was 9.3 days, and 9 cases (81.8%) with a duration of abdominal pain over 7 days. 3 cases of HSP with GP were treated by gastric/intestinal perforation repair and the other 8 cases were treated by enterectomy. The type of purpura, abdominal pain lasting more than 7 days, hematochezia, renal damage, and methylprednisolone dose more than 2mg/kg in GP with HSP patients show statistically significant compared with the control group (P<0.05). Conclusion: The incidence rate of GP in children with HSP was 0.10%. Abdominal (or mixed) HSP, hematochezia, renal damage, abdominal pain lasting more than 7 days, and methylprednisolone dose more than 2mg/kg may increase the risk of GP in children with HSP. CT has a high sensitivity for the diagnosis of GP. Early diagnosis and timely treatment of HSP with GP were very important for good clinical outcomes.

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Prediction of Renal Damage in Children with Henoch-Schönlein Purpura Based on Machine Learning

10.21203/rs.3.rs-1202084/v1 ◽

2022 ◽

Author(s):

Jinjuan Wang ◽

Huimin Chu ◽

Yueli Pan

Keyword(s):

Random Forest ◽

Renal Damage ◽

Recall Rate ◽

Support Vector ◽

Random Forest Algorithm ◽

Henoch Schonlein Purpura ◽

Accuracy Rate ◽

Schonlein Purpura ◽

Henoch Schönlein Purpura ◽

Purpura Nephritis

Abstract Background This article is objected to explore the value of machine learning algorithm in predicting the risk of renal damage in children with Henoch-Schönlein Purpura, and to construct a predictive model of Henoch-Schönlein Purpura Nephritis in children and analyze the related risk factors of Henoch-Schönlein Purpura Nephritis in children. Methods Case data of 288 hospitalized children with Henoch-Schönlein Purpura from November 2018 to October 2021 were collected. The data included 42 indicators such as demographic characteristics, clinical symptoms and laboratory tests, etc. Univariate feature selection was used for feature extraction, and Logistic regression, support vector machine, decision tree and random forest algorithm were used respectively for classification prediction. Last, the performance of four algorithms are compared using accuracy rate and recall rate. Results The accuracy rate, recall rate and AUC of the established random forest model were 0.83, 0.86 and 0.91 respectively, which were higher than 0.74, 0.80 and 0.89 of the Logistic regression model; higher than 0.70, 0.80 and 0.89 of support vector machine model; higher than 0.74, 0.80 and 0.81 of the decision tree model. The top 10 important features provided by random forest model are Persistent purpura≥4weeks, Cr, Clinic time, ALB, WBC, TC, TG, Relapse, TG, Recurrent purpura and EB-DNA. Conclusion The model based on random forest algorithm has better performance in the prediction of children with allergic purpura renal damage, indicated by better classification accuracy, better classification effect and better generalization performance.

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Abnormal B Cell Compartment Associated With Tfh Cells in Children With Henoch-schonlein Purpura

10.21203/rs.3.rs-44338/v1 ◽

2020 ◽

Author(s):

Ning Zhang ◽

Ge Tian ◽

Yuanyuan Sun ◽

Jing Pan ◽

Wei Xu ◽

...

Keyword(s):

B Cells ◽

B Cell ◽

Mononuclear Cells ◽

Healthy Controls ◽

Henoch Schonlein Purpura ◽

Cell Compartment ◽

Tfh Cells ◽

B Cell Subsets ◽

Schonlein Purpura ◽

Henoch Schönlein Purpura

Abstract Aim IgA-producing B cells were found to be associated with children diagnosed with Henoch-Schonlein purpura (HSP). The present study aimed to determine whether children with HSP possess abnormal B cell subsets. Methods A total of 14 children diagnosed with HSP, and age- and gender-matched healthy controls were enrolled in our study. Peripheral blood mononuclear cells were isolated, and the percentage of B cells subsets and Tfh cells were determined by flow cytometry. Finally, Spearman’s correlation coefficient was used to analyze the correlation between the percentage of Tfh cells and B cell subsets. Results We found that the frequency of total B cells was significantly increased in children with HSP; however, the percentage of plasma cells was significantly lower in HSP children. A significant reduction in the count of naïve B cells and an increase in class-switched B cells were found in children with HSP compared with healthy controls. We observed that the expression of C-X-C chemokine receptor type 5 (CXCR5) on total CD4+ T cells and the percentage of CD4+CXCR5+ cells were significantly increased within HSP patients. Moreover, significant correlations between Tfh cells and various B cells subsets were observed. Conclusion Our study showed a Tfh cell-associated abnormal B cell compartment in HSP children.

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