scholarly journals Effect of Panax notoginseng Saponins and Major Anti-Obesity Components on Weight Loss

2021 ◽  
Vol 11 ◽  
Author(s):  
Xuelian Zhang ◽  
Bin Zhang ◽  
Chenyang Zhang ◽  
Guibo Sun ◽  
Xiaobo Sun
Keyword(s):  
Weight Loss ◽  
Lipid Synthesis ◽  
Panax Notoginseng ◽  
Panax Notoginseng Saponins ◽  
Prevention And Treatment ◽  
Treatment Of Obesity ◽  
Obesity Drugs ◽  
Tissue Browning

The prevalence of individuals who are overweight or obese is rising rapidly globally. Currently, majority of drugs used to treat obesity are ineffective or are accompanied by obvious side effects; hence, the options are very limited. Therefore, it is necessary to find more effective and safer anti-obesity drugs. It has been proven in vivo and in vitro that the active ingredient notoginsenosides isolated from traditional Chinese medicine Panax notoginseng (Burk.) F. H. Chen exhibits anti-obesity effects. Notoginsenosides can treat obesity by reducing lipid synthesis, inhibiting adipogenesis, promoting white adipose tissue browning, increasing energy consumption, and improving insulin sensitivity. Although notoginsenosides are potential drugs for the treatment of obesity, their effects and mechanisms have not been analyzed in depth. In this review, the anti-obesity potential and mechanism of action of notoginsenosides were analyzed; thus laying emphasis on the timely prevention and treatment of obesity.

Panax notoginseng saponins provide neuroprotection by regulating NgR1/RhoA/ROCK2 pathway expression, in vitro and in vivo

2016 ◽  
Vol 190 ◽  
pp. 301-312 ◽  
Author(s):  
Xiaowei Shi ◽  
Wenjing Yu ◽  
Tiantian Yang ◽  
Wei Liu ◽  
Yizhou Zhao ◽  
...  
Keyword(s):  
Panax Notoginseng ◽  
Panax Notoginseng Saponins ◽  
Pathway Expression

scholarly journals Panax Notoginseng Saponins Reverse Steroid Resistance in Lupus Nephritis: Involvement of the Suppression of Exosomal P-gp Levels From Lymphocytes to Glomerular Endothelial Cells

2021 ◽  
Author(s):  
Feng Pan ◽  
Ying Lu
Keyword(s):  
Endothelial Cells ◽  
Lupus Nephritis ◽  
Panax Notoginseng ◽  
Scientific Basis ◽  
Steroid Resistance ◽  
Panax Notoginseng Saponins ◽  
Inflammatory Injury ◽  
Glomerular Endothelial Cells

Abstract Backgrounds: Microangiopathy is the most basic pathological manifestation of lupus nephritis (LN), and glomerular endothelial cells (GECs) injury is an important pathological mechanism. LN patients with microangiopathy are prone to steroid resistance (SR). Our previous studies confirmed that Panax notoginseng saponins (PNS) could reverse SR by downregulating the expression of P-gp in SR lymphocytes of LN mice (SLCsL/S). However, the mechanism of how circulating lymphocytes transmit SR information to GECs and thus affect the efficacy of kidney treatment is not clear. Recent studies have found that exosomes (exos) are an important carrier for intercellular bioactive substance communication. The aim of the study is to investigate whether exosomes derived from SLCsL/S mediate SR in GECs and PNS intervention.Methods: Exosomes isolated from SLCsL/S were characterized, and in vitro cell coculture was further conducted to investigate the effect of SLCsL/S-derived exosomes in the SR of GECs and PNS intervention. Sequencing was used to define the exosomal miRNA expression profiling of SR GECs. Moreover, the in vivo experiments were performed through the injection of exosomes extracted from SLCsL/S into the tail vein of mice.Results: In this study, we showed that exosomes derived from SLCsL/S could transmit SR information to GECs and lead to the aggravation of inflammatory injury through conferring P-gp, which were negated by a P-gp inhibitor. Further, we identified higher levels of exosomal miR-125b-5p from SR GECs were associated with SR in LN and could serve as biomarker for the risk of developing SR. PNS could reverse the SR of GECs and alleviate inflammatory injury by suppressing exosomal P-gp levels from lymphocytes to GECs in vitro and in vivo.Conclusions: Our findings suggest that exosomal transfer of SLCsL/S derived P-gp confer SR to GECs, and PNS can target exosome communication to reverse SR in LN, which provides new ideas and a scientific basis for improving the clinical efficacy of traditional Chinese medicine in the treatment of refractory LN.

scholarly journals Computational evidence of a compound with nicotinic α4β2-Ach receptor partial agonist properties as possible coadjuvant for the treatment of obesity

2016 ◽  
Author(s):  
Helena den-Haan ◽  
Juan José Hernández Morante ◽  
Horacio Pérez-Sánchez
Keyword(s):  
In Silico ◽  
Partial Agonist ◽  
Scoring Function ◽  
Pharmacophore Model ◽  
Functional Activities ◽  
Complex Disorder ◽  
Treatment Of Obesity ◽  
Obesity Drugs

ABSTRACTBackgroundNowadays, the search for new anti-obesity drugs is oriented to the use of anti-addiction medications like bupropion and naltrexone. Other compounds like varenicline may be also useful to treat obesity. However, the low effectiveness of the former or the high number of adverse effects of the latter makes it necessary to search for new therapeutic agents.MethodsScreening database selected for the computational experiments was DrugBank. 3D global shape comparison with varenicline was performed by means of the Ligand Based Virtual Screening tool WEGA v2015. A pharmacophore model based in the structure of varenicline was created by means of LigandScout v4.08. The in-silico screening was performed using Relative Pharmacophore Fit (RPF) scoring function implemented in LigandScout. Up to 3 mismatches with varenicline pharmacophore model were allowed for hits retrieving.ResultsDrugbank database was screened in silico to find alternative molecules to varenicline, and the compound cevimeline was found to have strong similarity to varenicline in terms of 3D shape and pharmacophoric features. Thus, we propose this hit may interact with nicotinic α4β2-Ach receptor in the same mode as varenicline does.DiscussionThe functional activities of this compound and its validity as a drug therapy for obesity treatment must be confirmed in further in vitro, in vivo and preclinical studies; however, attending to our screening procedure, this compound should be a promising therapy for such a complex disorder such as obesity.

scholarly journals Panax notoginseng Saponins Promotes Stroke Recovery by Influencing Expression of Nogo-A, NgR and p75NGF, in Vitro and in Vivo

2014 ◽  
Vol 37 (4) ◽  
pp. 560-568 ◽  
Author(s):  
Lixing Liu ◽  
Lingqun Zhu ◽  
Yihuai Zou ◽  
Wei Liu ◽  
Xiaoqian Zhang ◽  
...  
Keyword(s):  
Panax Notoginseng ◽  
Stroke Recovery ◽  
Panax Notoginseng Saponins

scholarly journals Panax Notoginseng Saponins Exert Anti-osteosarcoma Effect by Inhibiting G0 / G1 Phase and Affecting p53-mediated Autophagy and Mitochondrial Apoptosis

2021 ◽  
Author(s):  
Guangtao Han ◽  
Ting Liu
Keyword(s):  
Cell Cycle ◽  
Panax Notoginseng ◽  
Osteosarcoma Cell ◽  
Osteosarcoma Cells ◽  
Panax Notoginseng Saponins ◽  
Active Components ◽  
Invasion And Migration ◽  
And Migration

Abstract BackgroundOsteosarcoma is the most common primary bone malignancy. Chemotherapy for osteosarcoma often induces severe complications to the patients. Thus, the identification of new effective antineoplastic agents with fewer side effects remain a necessity. Panax notoginseng saponins (PNS) were therapeutic active components of panax notoginseng and were reported taking the capability to inhibit the growth of several tumors in vitro and in vivo. However, its effect on osteosarcoma has not been studied. This study first investigated the effect of PNS on osteosarcoma cells.MethodsCCK-8 essay used to determine the appropriate working concentration of PNS on osteosarcoma,annixV-FITC/PI experiment used to measure the apoptosis of PNS on osteosarcoma, wound healing assay was used to detect the migration of PNS on osteosarcoma, cell invasiveness was measured by transwell essay,cell cycle was measured by PI,the expression of relative protein was shown by western blot.ResultsOur result indicated that PNS inhibited osteosarcoma cells’ proliferation, invasion and migration, promoted their apoptosis. Besides, PNS also increased mitochondrial membrane potential and the level of reactive oxygen species. Cell cycle of osteosarcoma was arrested in G0 / G1 phase after treatment with PNS. The expression of p53, and mitochondrial related apoptosis proteins were promoted; however, decreased autophagy in osteosarcoma cells with PNS treatment were observed.ConclusionTaking the above effect of PNS on osteosarcoma, PNS were of the potential therapeutic value for treatment of osteosarcoma.

scholarly journals Panax notoginseng saponins reverse P-gp-mediated steroid resistance in lupus: involvement in the suppression of the SIRT1/FoxO1/MDR1 signalling pathway in lymphocytes

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Feng Pan ◽  
Yue-jin Li ◽  
Ying Lu
Keyword(s):  
Annexin V ◽  
Panax Notoginseng ◽  
Serum Levels ◽  
Underlying Mechanism ◽  
Signalling Pathway ◽  
Steroid Resistance ◽  
Panax Notoginseng Saponins ◽  
Apoptotic Effect

Abstract Background P-glycoprotein (P-gp)-mediated steroid resistance (SR) has been suggested to play a significant role in lupus nephritis (LN) treatment failure. Panax notoginseng saponins (PNS), the main effective components of the traditional Chinese medicine notoginseng, exhibited potent reversal capability of P-gp-mediated SR, but its mechanism remains unknown. This study aimed to investigate the effect of PNS on reversing SR in lupus and its underlying mechanism in vivo and in vitro. Methods In this study, an SR animal and splenic lymphocyte model were established using low-dose methylprednisolone (MP). Flow cytometry was used to detect the effect of PNS on reversing P-gp-mediated SR and the expression of P-gp in different T-cells phenotypes. Serum levels of ANA and dsDNA in lupus mice were measured by ELISA. Apoptosis was identified by Annexin V-FITC/PI staining. RT–PCR and Western blotting were used to detect the protein and mRNA expression levels of SIRT1, FoxO1, and MDR1 in SR splenic lymphocytes from lupus mice (SLCs/MPs). Results PNS could reverse the SR in lupus mice. Simultaneously, PNS increased the apoptotic effect of MP on SLCs/MP cells. The increased accumulation of rhodamine-123 (Rh-123) indicated that intracellular steroid accumulation could be increased by the action of PNS. Moreover, PNS decreased the expression of P-gp levels. Further experiments elucidated that the SIRT1/FoxO1/MDR1 signalling pathway existed in SLCs/MP cells, and PNS suppressed its expression level to reverse SR. The expression of P-gp in Th17 from SLCs/MP cells was increased, while PNS could reduce its level in a more obvious trend. Conclusion The present study suggested that PNS reversed P-gp-mediated SR via the SIRT1/FoxO1/MDR1 signalling pathway, which might become a valuable drug for the treatment of SR in lupus. Th17 might be the main effector cell of PNS reversing SR.

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