scholarly journals Molecular Cloning and Characterization of Sirtuin 1 and Its Potential Regulation of Lipid Metabolism and Antioxidant Response in Largemouth Bass (Micropterus salmoides)

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuting Huang ◽  
Shilin Wang ◽  
Xiaoxue Meng ◽  
Naisong Chen ◽  
Songlin Li
Keyword(s):  
Lipid Metabolism ◽  
Antioxidant Capacity ◽  
Binding Site ◽  
Largemouth Bass ◽  
Micropterus Salmoides ◽  
Antioxidant Response ◽  
Sirtuin 1 ◽  
Transcriptional Level ◽  
Multiple Sequence ◽  
Expression Of Genes

Sirtuin 1 (SIRT1) of largemouth bass (Micropterus salmoides) was cloned and characterized in the present study and the influence of SIRT1 activation induced by resveratrol inclusion on the expression of genes related to lipid metabolism and antioxidation was also investigated. The SIRT1 of largemouth bass, with full-length cDNA sequence of 3395bp encoding 695 amino acids, was mainly expressed in gonad, heart and liver. The analysis of multiple sequence alignment revealed that, in accordance with other species, SIRT1 of largemouth bass contained highly conserved substrate-binding site and NAD+ binding site. The result of subcellular localization displayed that SIRT1 of largemouth bass was mainly localized in the nucleus. The inclusion of 1.0 and 2.5‰ dietary RSV, a natural SIRT1 activator, significantly elevated the SIRT1 protein expression. Meanwhile, the phosphorylation of AKT1 and FoxO1 followed similar pattern with that of SIRT1, indicating the activation of insulin pathway, which may result in the inhibition of lipogenesis and activation of lipolysis, and reduced hepatic triglycerides content. Additionally, the activation of SIRT1 induced by dietary RSV elevated the antioxidant capacity at both transcriptional level and enzymatic level, which was probably mediated by the transcription factor Nrf2. In above, SIRT1 was suggested to be involved in improving antioxidant capacity and alleviating hepatic lipid deposition in largemouth bass.

scholarly journals Impact of dietary vitamin c on plasma metabolites, antioxidant capacity and innate immunocompetence in juvenile largemouth bass, Micropterus salmoides

2020 ◽  
Vol 17 ◽  
pp. 100383 ◽  
Author(s):  
Abdullateef Yusuf ◽  
Xuxiong Huang ◽  
Naisong Chen ◽  
Andrews Apraku ◽  
Weilong Wang ◽  
...  
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Largemouth Bass ◽  
Micropterus Salmoides ◽  
Dietary Vitamin ◽  
Plasma Metabolites

Resveratrol inclusion alleviated high dietary carbohydrate induced glycogen deposition and immune response of largemouth bass, Micropterus salmoides

2021 ◽  
pp. 1-33 ◽  
Author(s):  
Yijun Liu ◽  
Ning Liu ◽  
An Wang ◽  
Naisong Chen ◽  
Songlin Li
Keyword(s):  
Immune Response ◽  
Inflammatory Cytokines ◽  
Largemouth Bass ◽  
Micropterus Salmoides ◽  
Sirtuin 1 ◽  
Hepatic Glycogen ◽  
Dietary Carbohydrate ◽  
Glycogen Accumulation ◽  
High Carbohydrate ◽  
Glycogen Deposition

Abstract Excessive hepatic glycogen accumulation commonly impairs hepatocytes function, and further produces negative effects on growth and health status of carnivorous fish. A 9-week feeding trial was conducted to explore the potential regulation of resveratrol on high carbohydrate induced glycogen deposition and immune response of largemouth bass. Results showed that high dietary carbohydrate (10% inclusion of starch) led to hepatic glycogen accumulation and postprandial hyperglycemia compared to the diet with 5% starch, which was both alleviated with the inclusion of resveratrol. The use of resveratrol promoted the expression of sirtuin 1, which was down-regulated by high dietary carbohydrate. Meanwhile, resveratrol inclusion promoted the expression of genes involved in insulin pathway and glycolysis, and inhibited the expression of gluconeogenesis related genes. Additionally, high dietary carbohydrate significantly reduced lysozyme content but increased complement C4 content, which were both reversed with resveratrol supplementation. Meanwhile, resveratrol inclusion inhibited the expression of pro-inflammatory cytokines, but promoted anti-inflammatory cytokines expression, compare to the high carbohydrate treatment. In conclusion, resveratrol inclusion was beneficial in alleviating high dietary carbohydrate induced glycogen accumulation and immune response in largemouth bass.

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