The inclusion of fenugreek seed extract aggravated hepatic glycogen accumulation through reducing the expression of genes involved in insulin pathway and glycolysis in largemouth bass, Micropterus salmoides

AbstractAquafeeds for carnivorous species face a nutritional–technological conundrum: containing sufficient starch to meet specific manufacturing requirements for binding, extrusion and expansion, but ideally containing as little starch as possible owing to their limited ability to utilise carbohydrates. The present study evaluated the effects of dietary starch with different amylose to amylopectin ratios and resistant starch contents on growth performance, hepatic glycogen accumulation and glucose metabolism of an important cultured carnivorous finfish, largemouth bass (Micropterus salmoides). A common starch source (α-cassava starch (CS)) was tested as is or after being enzymatically de-branched at three different inclusion levels in diets for largemouth bass. Results showed that the increased dietary starch levels compromised performance and high dietary α-CS content led to obvious liver damage. However, the growth performances of fish fed the diets with de-branched starch (DS) were improved, and no manifest liver damages were observed even at the higher inclusion level. The increasing dietary starch contents significantly increased hepatic glycogen accumulation, but not when DS was used. High dietary starch content, without regard to starch sources, had no effect on the expression of glucose metabolism-related genes, except for down-regulation of insulin receptor expression. However, the use of dietary DS promoted the expression of genes involved in the insulin pathway and glycolysis. In conclusion, this study showed that the use of starch sources with a high amylose to amylopectin ratio and resistant starch in the feed for cultured carnivorous finfish could alleviate the hepatic glycogen deposition through regulating the insulin pathway and glycolysis.

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Resveratrol inclusion alleviated high dietary carbohydrate induced glycogen deposition and immune response of largemouth bass, Micropterus salmoides

British Journal Of Nutrition ◽

10.1017/s0007114521000544 ◽

2021 ◽

pp. 1-33 ◽

Cited By ~ 1

Author(s):

Yijun Liu ◽

Ning Liu ◽

An Wang ◽

Naisong Chen ◽

Songlin Li

Keyword(s):

Immune Response ◽

Inflammatory Cytokines ◽

Largemouth Bass ◽

Micropterus Salmoides ◽

Sirtuin 1 ◽

Hepatic Glycogen ◽

Dietary Carbohydrate ◽

Glycogen Accumulation ◽

High Carbohydrate ◽

Glycogen Deposition

Abstract Excessive hepatic glycogen accumulation commonly impairs hepatocytes function, and further produces negative effects on growth and health status of carnivorous fish. A 9-week feeding trial was conducted to explore the potential regulation of resveratrol on high carbohydrate induced glycogen deposition and immune response of largemouth bass. Results showed that high dietary carbohydrate (10% inclusion of starch) led to hepatic glycogen accumulation and postprandial hyperglycemia compared to the diet with 5% starch, which was both alleviated with the inclusion of resveratrol. The use of resveratrol promoted the expression of sirtuin 1, which was down-regulated by high dietary carbohydrate. Meanwhile, resveratrol inclusion promoted the expression of genes involved in insulin pathway and glycolysis, and inhibited the expression of gluconeogenesis related genes. Additionally, high dietary carbohydrate significantly reduced lysozyme content but increased complement C4 content, which were both reversed with resveratrol supplementation. Meanwhile, resveratrol inclusion inhibited the expression of pro-inflammatory cytokines, but promoted anti-inflammatory cytokines expression, compare to the high carbohydrate treatment. In conclusion, resveratrol inclusion was beneficial in alleviating high dietary carbohydrate induced glycogen accumulation and immune response in largemouth bass.

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Effects of dietary carbohydrate sources on growth performance, glycogen accumulation, insulin signaling pathway and hepatic glucose metabolism in largemouth bass, Micropterus salmoides

Aquaculture ◽

10.1016/j.aquaculture.2019.734391 ◽

2019 ◽

Vol 513 ◽

pp. 734391 ◽

Cited By ~ 12

Author(s):

Songlin Li ◽

Chunyan Sang ◽

An Wang ◽

Jiacan Zhang ◽

Naisong Chen

Keyword(s):

Glucose Metabolism ◽

Growth Performance ◽

Signaling Pathway ◽

Insulin Signaling ◽

Largemouth Bass ◽

Micropterus Salmoides ◽

Dietary Carbohydrate ◽

Glycogen Accumulation ◽

Hepatic Glucose Metabolism ◽

Hepatic Glucose

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Effects of different lipid sources on growth performance, fatty acids composition in tissue and expression of genes related to lipid metabolism in largemouth bass (Micropterus salmoides)

Aquaculture Reports ◽

10.1016/j.aqrep.2022.101013 ◽

2022 ◽

Vol 23 ◽

pp. 101013

Author(s):

Chao Liang ◽

Xiaoyi Zhao ◽

Lefei Jiao ◽

Yuedong Shen ◽

Jiaxiang Luo ◽

...

Keyword(s):

Fatty Acids ◽

Lipid Metabolism ◽

Growth Performance ◽

Largemouth Bass ◽

Micropterus Salmoides ◽

Fatty Acids Composition ◽

Expression Of Genes

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Molecular Cloning and Characterization of Sirtuin 1 and Its Potential Regulation of Lipid Metabolism and Antioxidant Response in Largemouth Bass (Micropterus salmoides)

Frontiers in Physiology ◽

10.3389/fphys.2021.726877 ◽

2021 ◽

Vol 12 ◽

Author(s):

Yuting Huang ◽

Shilin Wang ◽

Xiaoxue Meng ◽

Naisong Chen ◽

Songlin Li

Keyword(s):

Lipid Metabolism ◽

Antioxidant Capacity ◽

Binding Site ◽

Largemouth Bass ◽

Micropterus Salmoides ◽

Antioxidant Response ◽

Sirtuin 1 ◽

Transcriptional Level ◽

Multiple Sequence ◽

Expression Of Genes

Sirtuin 1 (SIRT1) of largemouth bass (Micropterus salmoides) was cloned and characterized in the present study and the influence of SIRT1 activation induced by resveratrol inclusion on the expression of genes related to lipid metabolism and antioxidation was also investigated. The SIRT1 of largemouth bass, with full-length cDNA sequence of 3395bp encoding 695 amino acids, was mainly expressed in gonad, heart and liver. The analysis of multiple sequence alignment revealed that, in accordance with other species, SIRT1 of largemouth bass contained highly conserved substrate-binding site and NAD+ binding site. The result of subcellular localization displayed that SIRT1 of largemouth bass was mainly localized in the nucleus. The inclusion of 1.0 and 2.5‰ dietary RSV, a natural SIRT1 activator, significantly elevated the SIRT1 protein expression. Meanwhile, the phosphorylation of AKT1 and FoxO1 followed similar pattern with that of SIRT1, indicating the activation of insulin pathway, which may result in the inhibition of lipogenesis and activation of lipolysis, and reduced hepatic triglycerides content. Additionally, the activation of SIRT1 induced by dietary RSV elevated the antioxidant capacity at both transcriptional level and enzymatic level, which was probably mediated by the transcription factor Nrf2. In above, SIRT1 was suggested to be involved in improving antioxidant capacity and alleviating hepatic lipid deposition in largemouth bass.

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