VvVHP1; 2 Is Transcriptionally Activated by VvMYBA1 and Promotes Anthocyanin Accumulation of Grape Berry Skins via Glucose Signal

AbstractBerry skin colour is a crucial determinant of red/black grape berry quality. We investigated the effects of combination treatments with amino acids and a low concentration of ABA on anthocyanin accumulation in grapes. Among the amino acids tested, isoleucine and phenylalanine resulted in high anthocyanin contents in grape cell cultures. The combination treatments with isoleucine or phenylalanine, and a low concentration of ABA enhanced anthocyanin accumulation in grape cells and detached grape berries. The combination treatment with isoleucine, but not with phenylalanine, and ABA upregulated MybA1 expression. Field-grown grapevines received combination treatments with isoleucine or phenylalanine, and ABA in two growing seasons. In the 2015 growing season, the combination treatments with isoleucine or phenylalanine, and a low concentration of ABA accelerated anthocyanin accumulation in grape berry skins of field-grown grapevines on days 10 and 31 post treatment. The effects on anthocyanin accumulation became negligible at harvest. The effect of the combination treatment with phenylalanine and a low concentration of ABA on anthocyanin accumulation was masked in the 2017 growing season due to the unexpected stimulation of anthocyanin accumulation by the low concentration of ABA, although the combination treatment accelerated anthocyanin accumulation on days 3 and 10 post treatment. Taken together, the results suggested that exogenous isoleucine and phenylalanine interacted with ABA-mediated anthocyanin accumulation in grape berry skins of field-grown grapevines when the activity of ABA used to treat the grapevines was inadequate.

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Characterization of the ABA Receptor VlPYL1 That Regulates Anthocyanin Accumulation in Grape Berry Skin

Frontiers in Plant Science ◽

10.3389/fpls.2018.00592 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 9

Author(s):

Zhen Gao ◽

Qin Li ◽

Jing Li ◽

Yujin Chen ◽

Meng Luo ◽

...

Keyword(s):

Grape Berry ◽

Anthocyanin Accumulation ◽

Berry Skin ◽

Aba Receptor

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Monitoring trans-Resveratrol in Grape Berry Skins During Ripening and in Corresponding Wines by HPLC

Food Technology and Biotechnology ◽

10.17113/ftb.53.01.15.3762 ◽

2015 ◽

Vol 53 (1) ◽

pp. 73-80 ◽

Cited By ~ 12

Author(s):

Elisabeta I. Geana ◽

◽

Oana R. Dinca ◽

Roxana E. Ionete ◽

Victoria Artem ◽

...

Keyword(s):

Grape Berry ◽

Berry Skins

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Use of image analysis for the study of phenolic compounds of the grape berry skin (Vitis vinifera L., cv Cabernet franc)

OENO One ◽

10.20870/oeno-one.2003.37.1.1681 ◽

2003 ◽

Vol 37 (1) ◽

pp. 61

Author(s):

Michel Chevalier ◽

Emilie Perrochon ◽

A. Clement ◽

Anne-Laure Dubot ◽

Marie Tellier ◽

...

Keyword(s):

Image Analysis ◽

Phenolic Compounds ◽

Grape Berry ◽

Berry Skin ◽

Good Tool ◽

Histological Techniques ◽

Berry Skins ◽

Quantitative Results ◽

Scion Image

<p style="text-align: justify;">The localization and quantitative determination of phenolics in grape berry skins, from the onset of veraison, constitute the first step to understand the évolution of these compounds throughout the maturation process. Histological techniques are appropriate to study the evolution of phenolics but manual countings are long and drudgery and do not allow for reliable quantitative results. The image analysis software "Scion Image" proved to be a good tool to improve the quantitative results. This method permitted also to measure the cells area and the area occupied by phenolic compounds inside the vacuoles. Image analysis could be helpful to the understanding of the évolution of phenolics during maturation and possibly contribute to explain their extraction during macération.</p>

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Resveratrols in Grape Berry Skins and Leaves in Vitis Germplasm

PLoS ONE ◽

10.1371/journal.pone.0061642 ◽

2013 ◽

Vol 8 (4) ◽

pp. e61642 ◽

Cited By ~ 14

Author(s):

Lijun Wang ◽

Man Xu ◽

Chunyan Liu ◽

Junfang Wang ◽

Huifen Xi ◽

...

Keyword(s):

Grape Berry ◽

Berry Skins

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Method for the Quantitative Extraction of Resveratrol and Piceid Isomers in Grape Berry Skins. Effect of Powdery Mildew on the Stilbene Content

Journal of Agricultural and Food Chemistry ◽

10.1021/jf000745o ◽

2001 ◽

Vol 49 (1) ◽

pp. 210-215 ◽

Cited By ~ 131

Author(s):

Ana I. Romero-Pérez ◽

Rosa M. Lamuela-Raventós ◽

Cristina Andrés-Lacueva ◽

M. Carmen de la Torre-Boronat

Keyword(s):

Powdery Mildew ◽

Grape Berry ◽

Quantitative Extraction ◽

Berry Skins

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Ethylene Induced by Sound Stimulation Enhances Anthocyanin Accumulation in Grape Berry Skin through Direct Upregulation of UDP-Glucose: Flavonoid 3-O-Glucosyltransferase

Cells ◽

10.3390/cells10102799 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2799

Author(s):

Mone Yamazaki ◽

Akari Ishida ◽

Yutaka Suzuki ◽

Yoshinao Aoki ◽

Shunji Suzuki ◽

...

Keyword(s):

Cultured Cells ◽

Early Stage ◽

Ethylene Biosynthesis ◽

Grape Berry ◽

Physiological Status ◽

Anthocyanin Accumulation ◽

Sound Stimulation ◽

Berry Skin ◽

Abscisic Acid Biosynthesis ◽

Promoter Assay

Global warming has resulted in the loss of anthocyanin accumulation in berry skin. Sound stimulation can be used as a potential method for enhancing fruit color development since many plants recognize sound vibration as an external stimulus and alter their physiological status in response to it. Sound stimulation (sine wave sound at 1000 Hz) enhanced anthocyanin accumulation in grape cultured cells and berry skins in field-grown grapevines at the early stage of ripening. The transcription of UFGT and ACO2, which encode the key enzymes in anthocyanin and ethylene biosynthesis, respectively, was upregulated in grape cultured cells exposed to sound stimulation. In contrast, the transcription of MybA1 and NCED1, which encode a transcription factor for UFGT and a key enzyme in abscisic acid biosynthesis, respectively, was not affected by the sound stimulation. A treatment with an ethylene biosynthesis inhibitor, aminoethoxyvinyl glycine hydrochloride, revered the enhancement of anthocyanin accumulation by sound stimulation. As the promoter assay using a GUS reporter gene demonstrated that UFGT promoter was directly activated by the ethylene-releasing compound ethephon, which enhanced anthocyanin accumulation in grape cultured cells, we conclude that sound stimulation enhanced anthocyanin accumulation through the direct upregulation of UFGT by ethylene biosynthesis. Our findings suggest that sound stimulation contributes to alleviating poor coloration in berry skin as a novel and innovative practical technique in viticulture.

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Preharvest Chitosan and Postharvest UV Irradiation Treatments Suppress Gray Mold of Table Grapes

Plant Disease ◽

10.1094/pd-90-0445 ◽

2006 ◽

Vol 90 (4) ◽

pp. 445-450 ◽

Cited By ~ 81

Author(s):

G. Romanazzi ◽

F. Mlikota Gabler ◽

J. L. Smilanick

Keyword(s):

Chitinase Activity ◽

Gray Mold ◽

Grape Berry ◽

Thompson Seedless ◽

Berry Skin ◽

Grape Berries ◽

Table Grapes ◽

Ultraviolet C ◽

Berry Skins ◽

Uv C

The effectiveness of chitosan treatment of table grapes, alone or in combination with ultraviolet-C (UV-C) radiation, to control postharvest gray mold caused by Botrytis cinerea, was determined in California, United States. The influence of these treatments on catechin and resveratrol contents and chitinase activity in grape berry skins also was assessed. Clusters of cvs. Thompson Seedless, Autumn Black, and Emperor were sprayed in the vineyard with 1% chitosan, then harvested daily for 5 days. Promptly after harvest, they were inoculated with B. cinerea. Decay incidence and disease severity were significantly reduced by chitosan, which was most effective on berries harvested 1 or 2 days after treatment. In another experiment, grape berries were sprayed in the vineyard with chitosan, harvested 2 days later, irradiated for 5 min with UV-C (0.36 J/cm2), and inoculated with B. cinerea 2 days later. Combined chitosan and UV-C treatments applied to cv. Autumn Black or selection B36-55 were synergistic in reducing gray mold incidence and severity compared with either treatment alone. Preharvest chitosan treatment increased neither concentration of catechin or resveratrol nor activity of chitinase in berry skin. Conversely, UV-C irradiation, alone or combined with chitosan treatment, induced catechin in cv. Autumn Black berries and trans-resveratrol in both cv. Autumn Black and selection B36-55.

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Influence of abscisic acid in triggering "véraison" in grape berry skins of Vitis vinifera L. cv. Cabernet-Sauvignon

OENO One ◽

10.20870/oeno-one.2006.40.1.882 ◽

2006 ◽

Vol 40 (1) ◽

pp. 7 ◽

Cited By ~ 4

Author(s):

Séverine Gagné ◽

Karine Esteve ◽

Christelle Deytieux-Belleau ◽

Cédric Saucier ◽

Laurence Geny

Keyword(s):

Abscisic Acid ◽

Phenylalanine Ammonia Lyase ◽

Grape Berry ◽

Hormonal Control ◽

Close Relationship ◽

Lyase Activity ◽

Berry Skins ◽

Phenylalanine Ammonia Lyase Activity ◽

Aba Content ◽

Grape Berry Ripening

<p style="text-align: justify;">Grape berry ripening is characterised by numerous metabolic and physiological changes, placed under hormonal control. The phytohormone abscisic acid (ABA) is considered as a possible promoter of « véraison », since it accumulates at this time. In order to evaluate its impact, we assess the effect of ABA on several parameters of maturity: anthocyanins synthesis, phenylalanine ammonia-lyase activity, sugar contents, acidity and maturity index, both in untreated berry skin and in skin of berries submitted to applications of exogenous ABA. The maturation processes we focused on appear ABA-dependent, as they occured immediately after ABA accumulation in the skin. Treatment also shows a close relationship between ABA and the beginning of ripening. These results allow us to conclude that ABA content could modulate the triggering of « véraison ».</p>

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