AP2/DREB Transcription Factor RAP2.4 Activates Cuticular Wax Biosynthesis in Arabidopsis Leaves Under Drought

ABSTRACTCellular functions are diversified through intricate transcription regulations, and an understanding gene regulation networks is essential to elucidating many developmental processes and environmental responses. Here, we employed the Transcriptional-Activator Like effectors (TALes), which represent a family of transcription factors that are synthesized by members of the γ-proteobacterium genus Xanthomonas and secreted to host cells for activation of targeted host genes. Through delivery by the maize pathogen, Xanthomonas vasicola pv. vasculorum, designer TALes (dTALes), which are synthetic TALes, were used to induce the expression of the maize gene glossy3 (gl3), a MYB transcription factor gene involved in the cuticular wax biosynthesis. RNA-Seq analysis of leaf samples identified 146 gl3 downstream genes. Eight of the nine known genes known to be involved in the cuticular wax biosynthesis were up-regulated by at least one dTALe. A top-down Gaussian graphical model predicted that 68 gl3 downstream genes were directly regulated by GL3. A chemically induced mutant of the gene Zm00001d017418 from the gl3 downstream gene, encoding aldehyde dehydrogenase, exhibited a typical glossy leaf phenotype and reduced epicuticular waxes. The bacterial protein delivery of artificial transcription factors, dTALes, proved to be a straightforward and powerful approach for the revelation of gene regulation in plants.

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Activation of Mitochondrial orf355 Gene Expression by a Nuclear-Encoded DREB Transcription Factor Causes Cytoplasmic Male Sterility in Maize

Molecular Plant ◽

10.1016/j.molp.2020.07.002 ◽

2020 ◽

Vol 13 (9) ◽

pp. 1270-1283 ◽

Cited By ~ 1

Author(s):

Senlin Xiao ◽

Jie Zang ◽

Yuanrong Pei ◽

Jie Liu ◽

Juan Liu ◽

...

Keyword(s):

Gene Expression ◽

Transcription Factor ◽

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Dreb Transcription Factor

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CBF/DREB transcription factor genes play role in cadmium tolerance and phytoaccumulation in Ricinus communis under molybdenum treatments

Chemosphere ◽

10.1016/j.chemosphere.2018.05.165 ◽

2018 ◽

Vol 208 ◽

pp. 425-432 ◽

Cited By ~ 11

Author(s):

Nasir Ali ◽

Fazal Hadi

Keyword(s):

Transcription Factor ◽

Ricinus Communis ◽

Cadmium Tolerance ◽

Dreb Transcription Factor ◽

Transcription Factor Genes

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The MYB96 Transcription Factor Regulates Cuticular Wax Biosynthesis under Drought Conditions in Arabidopsis

The Plant Cell ◽

10.1105/tpc.111.083485 ◽

2011 ◽

Vol 23 (3) ◽

pp. 1138-1152 ◽

Cited By ~ 283

Author(s):

Pil Joon Seo ◽

Saet Buyl Lee ◽

Mi Chung Suh ◽

Mi-Jeong Park ◽

Young Sam Go ◽

...

Keyword(s):

Transcription Factor ◽

Cuticular Wax ◽

Drought Conditions

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Genome-wide identification and characterization of the DREB transcription factor gene family in mulberry

Biologia Plantarum ◽

10.1007/s10535-015-0498-x ◽

2015 ◽

Vol 59 (2) ◽

pp. 253-265 ◽

Cited By ~ 11

Author(s):

X. Q. Liu ◽

J. J. Zhu ◽

C. J. Wei ◽

Q. Guo ◽

C. K. Bian ◽

...

Keyword(s):

Transcription Factor ◽

Gene Family ◽

Transcription Factor Gene ◽

Factor Gene ◽

Genome Wide ◽

Dreb Transcription Factor ◽

Identification And Characterization ◽

Transcription Factor Gene Family

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Cuticular wax biosynthesis is positively regulated by WRINKLED4, an AP2/ERF-type transcription factor, in Arabidopsis stems

The Plant Journal ◽

10.1111/tpj.13248 ◽

2016 ◽

Vol 88 (2) ◽

pp. 257-270 ◽

Cited By ~ 31

Author(s):

Chan Song Park ◽

Young Sam Go ◽

Mi Chung Suh

Keyword(s):

Transcription Factor ◽

Cuticular Wax

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Response of DREB transcription factor to drought stress based on DNA methylation in wheat

10.21203/rs.2.22506/v1 ◽

2020 ◽

Author(s):

Yanqiu Zhu ◽

Huihui Wang ◽

Wenjing Jia ◽

Xiaoyan Wei ◽

Zhikun Duan ◽

...

Keyword(s):

Gene Expression ◽

Dna Methylation ◽

Transcription Factor ◽

Drought Stress ◽

Promoter Methylation ◽

Resistance Mechanism ◽

Dreb Transcription Factor ◽

The Status ◽

Element Binding Protein ◽

Responsive Element

Abstract Background: The growth and development of wheat are seriously influenced by drought stress, and the research on drought resistance mechanism of wheat is very important. Dehydration responsive element binding protein (DREB) plays an important role in plant response to drought stress, but epigenetic regulation for gene expression of DREB transcription factor is less studied, especially the regulatory role of DNA methylation has not been reported.Results: In this research, DREB2, DREB6 and Wdreb2 were cloned from wheat in this study, their CDS sequence was composed of 732bp, 837bp or 1035bp, respectively, one 712bp intron was found in DREB6. Although AP2/EREBP domain of DREB2, DREB6 and Wdreb2 had 73.25% identity, they belong to different types of DREB transcription factor, and the expression of Wdreb2 was significantly higher, yet was the lowest in DREB2. Under drought stress, the expression of DREB2, DREB6 and Wdreb2 could be induced, but had different trends along with the increase of stress time, and their expression had tissue specificity, was obviously higher in leaf. Promoter of DREB2, DREB6 and Wdreb2 in leaf was further studied, some elements related to adverse stress were found, and the promoter of DREB2 and Wdreb2 was slightly methylated, but DREB6 promoter was mildly methylated. Compared with the control, the level of promoter methylation decreased in DREB2 and DREB6 as stressed for 2h, then increased along with the increase of stress time, which was opposite in Wdreb2 promoter, the status of promoter methylation also had significant change under drought stress. Further analysis showed that promoter methylation of DREB6 or Wdreb2 was negatively correlated with their expression, especially was significant in Wdreb2. Conclusions: DREB2, DREB6 and Wdreb2 might function differently in response to drought stress, and promoter methylation had more significant effects on gene expression of Wdreb2 and DREB6.

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Increased cuticular wax accumulation and enhanced drought tolerance in transgenic alfalfa (Medicago sativa) by overexpression of a transcription factor gene

NZGA: Research and Practice Series ◽

10.33584/rps.12.2006.3024 ◽

2006 ◽

Vol 12 ◽

pp. 145-150

Author(s):

J.I.-Yi Zhang ◽

Mary Sledge ◽

Joseph Bouton ◽

Zeng-Y.U. Wang

Keyword(s):

Transcription Factor ◽

Drought Tolerance ◽

Medicago Sativa ◽

Cuticular Wax ◽

Transcription Factor Gene ◽

Cuticular Waxes ◽

Model Legume ◽

Factor Gene ◽

Transgenic Alfalfa ◽

Ap2 Domain

Plant cuticular waxes play an important role in protecting aerial organs from damage caused by multiple environmental stresses such as drought, cold, UV radiation, pathogen infection, and insect attack. We characterized a novel AP2 domain-containing transcription factor gene, designated WXP1, from the model legume plant Medicago truncatula. The gene is able to activate wax production and confer drought tolerance in alfalfa (Medicago sativa). The predicted protein of WXP1 has 371 aa; it is one of the longest peptides of all the single AP2 domain proteins in M. truncatula. Transcript level of WXP1 is inducible by cold, ABA and drought treatment in shoot tissues. Overexpression of WXP1 under the control of CaMV35S promoter led to a significant increase in cuticular wax loading on leaves of transgenic alfalfa. Transgenic leaves showed reduced water loss and chlorophyll leaching. Transgenic alfalfa plants with increased cuticular waxes showed enhanced drought tolerance demonstrated by delayed wilting after watering was ceased and quicker and better recovery when the dehydrated plants were re-watered.

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