Digging for Stress-Responsive Cell Wall Proteins for Developing Stress-Resistant Maize

Gordonia jacobaea was isolated and characterized in the Department of Microbiology, University of Santiago de Compostela, in 2000. Here we present the draft genome sequence of this species, which will improve our understanding of the diversity and the relation of the cell wall proteins of G. jacobaea with other mycolata.

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Proteomic Analysis ofPhytophthora infestansReveals the Importance of Cell Wall Proteins in Pathogenicity

Molecular & Cellular Proteomics ◽

10.1074/mcp.m116.065656 ◽

2017 ◽

Vol 16 (11) ◽

pp. 1958-1971 ◽

Cited By ~ 10

Author(s):

Svante Resjö ◽

Maja Brus ◽

Ashfaq Ali ◽

Harold J. G. Meijer ◽

Marianne Sandin ◽

...

Keyword(s):

Cell Wall ◽

Proteomic Analysis ◽

Cell Wall Proteins

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A proposed nomenclature for cell wall proteins of Clostridium difficile

Journal of Medical Microbiology ◽

10.1099/jmm.0.028472-0 ◽

2011 ◽

Vol 60 (8) ◽

pp. 1225-1228 ◽

Cited By ~ 38

Author(s):

Robert P. Fagan ◽

Claire Janoir ◽

Anne Collignon ◽

Paola Mastrantonio ◽

Ian R. Poxton ◽

...

Keyword(s):

Cell Wall ◽

Clostridium Difficile ◽

Cell Wall Proteins

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Homologous Recombination in Clostridioides difficile Mediates Diversification of Cell Surface Features and Transport Systems

mSphere ◽

10.1128/msphere.00799-20 ◽

2020 ◽

Vol 5 (6) ◽

Author(s):

Hannah D. Steinberg ◽

Evan S. Snitkin

Keyword(s):

Cell Wall ◽

Homologous Recombination ◽

Sugar Transport ◽

The United States ◽

Strain Typing ◽

Cell Wall Proteins ◽

Genetic Loci ◽

Content Type ◽

Clostridioides Difficile ◽

Host Pathogen

ABSTRACT Illness caused by the pathogen Clostridioides difficile is widespread and can range in severity from mild diarrhea to sepsis and death. Strains of C. difficile isolated from human infections exhibit great genetic diversity, leading to the hypothesis that the genetic background of the infecting strain at least partially determines a patient’s clinical course. However, although certain strains of C. difficile have been suggested to be associated with increased severity, strain typing alone has proved insufficient to explain infection severity. The limited explanatory power of strain typing has been hypothesized to be due to genetic variation within strain types, as well as genetic elements shared between strain types. Homologous recombination is an evolutionary mechanism that can result in large genetic differences between two otherwise clonal isolates, and also lead to convergent genotypes in distantly related strains. More than 400 C. difficile genomes were analyzed here to assess the effect of homologous recombination within and between C. difficile clades. Almost three-quarters of single nucleotide variants in the C. difficile phylogeny are predicted to be due to homologous recombination events. Furthermore, recombination events were enriched in genes previously reported to be important to virulence and host-pathogen interactions, such as flagella, cell wall proteins, and sugar transport and metabolism. Thus, by exploring the landscape of homologous recombination in C. difficile, we identified genetic loci whose elevated rates of recombination mediated diversification, making them strong candidates for being mediators of host-pathogen interaction in diverse strains of C. difficile. IMPORTANCE Infections with C. difficile result in up to half a million illnesses and tens of thousands of deaths annually in the United States. The severity of C. difficile illness is dependent on both host and bacterial factors. Studying the evolutionary history of C. difficile pathogens is important for understanding the variation in pathogenicity of these bacteria. This study examines the extent and targets of homologous recombination, a mechanism by which distant strains of bacteria can share genetic material, in hundreds of C. difficile strains and identifies hot spots of realized recombination events. The results of this analysis reveal the importance of homologous recombination in the diversification of genetic loci in C. difficile that are significant in its pathogenicity and host interactions, such as flagellar construction, cell wall proteins, and sugar transport and metabolism.

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Slipped strand mispairing in the gene encoding sialidase NanH3 in Gardnerella spp

10.1101/2020.09.16.300947 ◽

2020 ◽

Author(s):

Shakya P. Kurukulasuriya ◽

Mo H. Patterson ◽

Janet E. Hill

Keyword(s):

Cell Wall ◽

Biofilm Formation ◽

Gene Sequence ◽

Phase Variation ◽

Cell Wall Proteins ◽

Vaginal Microbiome ◽

Gene Encoding ◽

Reading Frame ◽

Sialidase Activity ◽

Mucosal Surfaces

AbstractCell wall proteins with sialidase activity are involved in carbohydrate assimilation, adhesion to mucosal surfaces, and biofilm formation. Gardnerella spp. inhabit the human vaginal microbiome and encode up to three sialidase enzymes, two of which are suspected to be cell wall associated. Here we demonstrate that the gene encoding extracellular sialidase NanH3 is found almost exclusively in G. piotii and closely related Gardnerella genome sp. 3, and its presence correlates with sialidase positive phenotype in a collection of 112 Gardnerella isolates. The nanH3 gene sequence includes a homopolymeric repeat of cytosines that varies in length within cell populations, indicating that this gene is subject to slipped-strand mispairing, a mechanisms of phase variation in bacteria. Variation in the length of the homopolymer sequence results in encoding of either the full length sialidase protein or truncated peptides lacking the sialidase domain due to introduction of reading-frame shifts and premature stop codons. Phase variation in NanH3 may be involved in immune evasion or modulation of adhesion to host epithelial cells, and formation of biofilms characteristic of the vaginal dysbiosis known as bacterial vaginosis.

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Defining Staphylococcus epidermidis cell wall proteins.

Journal of Clinical Microbiology ◽

10.1128/jcm.28.12.2757-2760.1990 ◽

1990 ◽

Vol 28 (12) ◽

pp. 2757-2760 ◽

Cited By ~ 8

Author(s):

C C Patrick ◽

M R Plaunt ◽

S M Sweet ◽

G S Patrick

Keyword(s):

Cell Wall ◽

Staphylococcus Epidermidis ◽

Cell Wall Proteins

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Arabidopsis Regenerating Protoplast: A Powerful Model System for Combining the Proteomics of Cell Wall Proteins and the Visualization of Cell Wall Dynamics

Proteomes ◽

10.3390/proteomes4040034 ◽

2016 ◽

Vol 4 (4) ◽

pp. 34 ◽

Cited By ~ 8

Author(s):

Ryusuke Yokoyama ◽

Hiroaki Kuki ◽

Takeshi Kuroha ◽

Kazuhiko Nishitani

Keyword(s):

Cell Wall ◽

Model System ◽

Cell Wall Proteins

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Enzymatic cell wall proteins in higher plants

Giornale botanico italiano ◽

10.1080/11263509509436126 ◽

1995 ◽

Vol 129 (1) ◽

pp. 221-229

Author(s):

Rodolfo Federico ◽

Maria Laurenzi ◽

Riccardo Angelini

Keyword(s):

Cell Wall ◽

Higher Plants ◽

Cell Wall Proteins

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Genome sequencing, assembly, and annotation of the self-flocculating microalga Scenedesmus obliquus AS-6-11

BMC Genomics ◽

10.1186/s12864-020-07142-4 ◽

2020 ◽

Vol 21 (1) ◽

Author(s):

Bai-Ling Chen ◽

Wuttichai Mhuantong ◽

Shih-Hsin Ho ◽

Jo-Shu Chang ◽

Xin-Qing Zhao ◽

...

Keyword(s):

Cell Wall ◽

Scenedesmus Obliquus ◽

The Self ◽

Cell Wall Proteins ◽

Green Microalgae ◽

Biotechnological Applications ◽

Volvox Carteri ◽

Chlorella Variabilis ◽

Genes Encoding ◽

Genomic Studies

Abstract Background Scenedesmus obliquus belongs to green microalgae and is widely used in aquaculture as feed, which is also explored for lipid production and bioremediation. However, genomic studies of this microalga have been very limited. Cell self-flocculation of microalgal cells can be used as a simple and economic method for harvesting biomass, and it is of great importance to perform genome-scale studies for the self-flocculating S. obliquus strains to promote their biotechnological applications. Results We employed the Pacific Biosciences sequencing platform for sequencing the genome of the self-flocculating microalga S. obliquus AS-6-11, and used the MECAT software for de novo genome assembly. The estimated genome size of S. obliquus AS-6-11 is 172.3 Mbp with an N50 of 94,410 bp, and 31,964 protein-coding genes were identified. Gene Ontology (GO) and KEGG pathway analyses revealed 65 GO terms and 428 biosynthetic pathways. Comparing to the genome sequences of the well-studied green microalgae Chlamydomonas reinhardtii, Chlorella variabilis, Volvox carteri and Micractinium conductrix, the genome of S. obliquus AS-6-11 encodes more unique proteins, including one gene that encodes D-mannose binding lectin. Genes encoding the glycosylphosphatidylinositol (GPI)-anchored cell wall proteins, and proteins with fasciclin domains that are commonly found in cell wall proteins might be responsible for the self-flocculating phenotype, and were analyzed in detail. Four genes encoding both GPI-anchored cell wall proteins and fasciclin domain proteins are the most interesting targets for further studies. Conclusions The genome sequence of the self-flocculating microalgal S. obliquus AS-6-11 was annotated and analyzed. To our best knowledge, this is the first report on the in-depth annotation of the S. obliquus genome, and the results will facilitate functional genomic studies and metabolic engineering of this important microalga. The comparative genomic analysis here also provides new insights into the evolution of green microalgae. Furthermore, identification of the potential genes encoding self-flocculating proteins will benefit studies on the molecular mechanism underlying this phenotype for its better control and biotechnological applications as well.

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