Iris domestica (iso)flavone 7- and 3′-O-Glycosyltransferases Can Be Induced by CuCl2

In many plants, isoflavones are the main secondary metabolites that have various pharmacological activities, but the low water solubility of aglycones limits their usage. The O-glycosylation of (iso)flavones is a promising way to overcome this barrier. O-glycosyltransferases (UGTs) are key enzymes in the biosynthesis of (iso)flavonoid O-glycosides in plants. However, limited investigations on isoflavonoid O-UGTs have been reported, and they mainly focused on legumes. Iris domestica (L.) Goldblatt et Mabberley is a non-legume plant rich in various isoflavonoid glycosides. However, there are no reports regarding its glycosylation mechanism, despite the I. domestica transcriptome previously being annotated as having non-active isoflavone 7-O-UGTs. Our previous experiments indicated that isoflavonoid glycosides were induced by CuCl2 in I. domestica calli; therefore, we hypothesized that isoflavone O-UGTs may be induced by Cu2+. Thus, a comparative transcriptome analysis was performed using I. domestica seedlings treated with CuCl2, and eight new active BcUGTs were obtained. Biochemical analyses showed that most of the active BcUGTs had broad substrate spectra; however, substrates lacking 5-OH were rarely catalyzed. Real-time quantitative PCR results further indicated that the transcriptional levels of BcUGTs were remarkably induced by Cu2+. Our study increases the understanding of UGTs and isoflavone biosynthesis in non-legume plants.

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Compared to Odorant-binding Proteins in the Reproductive System and Antennae of Athetis Dissimilis using Transcriptome Analysis

10.21203/rs.3.rs-199888/v1 ◽

2021 ◽

Author(s):

Yue-Qin Song ◽

Zhi-Yu Song ◽

Jun-Feng Dong ◽

Qi-Hui Lv ◽

Qing-Xiao Chen ◽

...

Keyword(s):

Quantitative Pcr ◽

Transcriptome Analysis ◽

Reproductive System ◽

Binding Proteins ◽

Reproductive Organs ◽

Odorant Binding Proteins ◽

Real Time Quantitative Pcr ◽

Lepidopteran Species ◽

Odorant Binding ◽

Different Tissues

Abstract Odorant-binding proteins (OBPs) are prevalent in the antennal transcriptomes of different orders of insects. Studies on OBPs have focused on their role in the insect chemosensory system, but knowledge of their functions in the insect testis is limited. We sequenced the transcriptomes of the Athetis dissimilis reproductive organs and analyzed the expressive of OBPs in different tissues. We identified 23 OBPs in the testis and ovaries and 31 OBPs in antennal transcriptomes. The results of real-time quantitative PCR revealed that 23 of the 54 OBP genes were highly expressed in both female and male antennae, including three that exhibited male-biased expression and 15 that exhibited female-biased expression. A total of 24 OBPs were highly expressed in the testis of A. dissimilis, while expression of OBPs in the ovaries was very low. These findings highlight the functional diversity of OBPs in insects and can facilitate further studies on the OBPs in A. dissimilis and lepidopteran species.

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Faculty Opinions recommendation of Comparative transcriptome analysis of human trophectoderm and embryonic stem cell-derived trophoblasts reveal key participants in early implantation.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.13370034.14740162 ◽

2011 ◽

Author(s):

Lois Salamonsen

Keyword(s):

Stem Cell ◽

Embryonic Stem Cell ◽

Transcriptome Analysis ◽

Embryonic Stem ◽

Comparative Transcriptome ◽

Comparative Transcriptome Analysis ◽

Key Participants

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Anticancer Activity of Natural Flavonoids: Inhibition of HIF-1α Signaling Pathway

Current Organic Chemistry ◽

10.2174/1385272823666191203122030 ◽

2020 ◽

Vol 23 (26) ◽

pp. 2945-2959 ◽

Cited By ~ 1

Author(s):

Xiangping Deng ◽

Yijiao Peng ◽

Jingduo Zhao ◽

Xiaoyong Lei ◽

Xing Zheng ◽

...

Keyword(s):

Transcription Factor ◽

Secondary Metabolites ◽

Anticancer Agents ◽

Hypoxia Inducible Factor ◽

Tumor Hypoxia ◽

Pharmacological Activities ◽

Hypoxia Inducible Factor 1 ◽

The Past ◽

Low Toxicity ◽

Tumor Blood Vessels

Rapid tumor growth is dependent on the capability of tumor blood vessels and glycolysis to provide oxygen and nutrients. Tumor hypoxia is a common characteristic of many solid tumors, and it essentially happens when the growth of the tumor exceeds the concomitant angiogenesis. Hypoxia-inducible factor 1 (HIF-1) as the critical transcription factor in hypoxia regulation is activated to adapt to this hypoxia situation. Flavonoids, widely distributed in plants, comprise many polyphenolic secondary metabolites, possessing broadspectrum pharmacological activities, including their potentiality as anticancer agents. Due to their low toxicity, intense efforts have been made for investigating natural flavonoids and their derivatives that can be used as HIF-1α inhibitors for cancer therapy during the past few decades. In this review, we sum up the findings concerning the inhibition of HIF-1α by natural flavonoids in the last few years and propose the idea of designing tumor vascular and glycolytic multi-target inhibitors with HIF-1α as one of the targets.

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Bioprospecting Cryptogams as Potential Source of Unique Biodynamic Phytochemicals with Diverse Pharmaceutical Applications

Cryptogam Biodiversity and Assessment ◽

10.21756/cba.v1i1.10959 ◽

2016 ◽

Vol 1 (1) ◽

Author(s):

Brahma N. Singh ◽

Garima Pandey ◽

Prateeksha ◽

J. Kumar

Keyword(s):

Secondary Metabolites ◽

Higher Plants ◽

New Drugs ◽

Terrestrial Plants ◽

Pharmacological Activities ◽

Therapeutic Drugs ◽

Potential Source ◽

Therapeutic Value ◽

Wide Range ◽

Novel Structures

With the advent of green pharmaceuticals, the secondary metabolites derived from plants have provided numerous leads for the development of a wide range of therapeutic drugs; however the discovery of new drugs with novel structures has declined in the past few years. Cryptogams including lichens, bryophytes, and pteridophytes represent a group of small terrestrial plants that remain relatively untouched in the drug discovery process though some have been used as ethnomedicines by various tribes worldwide. Studies of their secondary metabolites are recent but reveal unique secondary metabolites which are not synthesized by higher plants. These compounds can have the potential to develop more potential herbal drugs for prevention and treatment of diseases The present article . deals with the secondary metabolites and pharmacological activities of cryptogams with an objective to bring them forth as potential source of biodynamic compounds of therapeutic value.

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