scholarly journals Mitogen-Activated Protein Kinase CaDIMK1 Functions as a Positive Regulator of Drought Stress Response and Abscisic Acid Signaling in Capsicum annuum

2021 ◽  
Vol 12 ◽  
Author(s):  
Minchae Kim ◽  
Soongon Jeong ◽  
Chae Woo Lim ◽  
Sung Chul Lee
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Capsicum Annuum ◽  
Stomatal Closure ◽  
Leaf Tissue ◽  
Mitogen Activated Protein Kinase ◽  
Growth Stages ◽  
Adult Growth ◽  
Mitogen Activated Protein ◽  
Pepper Plants

Protein phosphorylation by kinase is an important mechanism for adapting to drought stress conditions. Here, we isolated the CaDIMK1 (Capsicum annuum drought-induced MAP kinase 1) from dehydrated pepper leaf tissue and functionally characterized it. Subcellular localization analysis revealed that the CaDIMK1 protein was localized in the cytoplasm and nucleus. CaDIMK1-silenced pepper plants exhibited drought-susceptible phenotypes that were characterized by increased transpiration rates, low leaf temperatures, and decreased stomatal closure. In contrast, CaDIMK1-overexpressing (OX) transgenic Arabidopsis plants were hypersensitive to abscisic acid (ABA) from germination to adult growth stages. Furthermore, the CaDIMK1-OX plants were tolerant to drought stress. The transcript levels of several stress-related genes were high in CaDIMK1-OX plants than in wild-type plants. Taken together, our data demonstrate that CaDIMK1 acts as a positive modulator of drought tolerance and ABA signal transduction in pepper plants.

scholarly journals Abscisic Acid Induces Mitogen-Activated Protein Kinase Activation in Barley Aleurone Protoplasts

1996 ◽  
Vol 8 (6) ◽  
pp. 1061 ◽  
Author(s):  
Menno L. W. Knetsch ◽  
Mei Wang ◽  
B. Ewa Snaar-Jagalska ◽  
Sjoukje Heimovaara-Dijkstra
Keyword(s):  
Abscisic Acid ◽  
Protein Kinase ◽  
Mitogen Activated Protein Kinase ◽  
Kinase Activation ◽  
Protein Kinase Activation ◽  
Mitogen Activated Protein ◽  
Barley Aleurone

scholarly journals AIK1, A Mitogen-Activated Protein Kinase, Modulates Abscisic Acid Responses through the MKK5-MPK6 Kinase Cascade

2016 ◽  
Vol 173 (2) ◽  
pp. 1391-1408 ◽  
Author(s):  
Kun Li ◽  
Fengbo Yang ◽  
Guozeng Zhang ◽  
Shufei Song ◽  
Yuan Li ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Protein Kinase ◽  
Mitogen Activated Protein Kinase ◽  
Mitogen Activated Protein ◽  
Kinase Cascade

scholarly journals Knockdown of a Novel Gene OsTBP2.2 Increases Sensitivity to Drought Stress in Rice

Genes ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 629
Author(s):  
Yong Zhang ◽  
Limei Zhao ◽  
Hong Xiao ◽  
Jinkiat Chew ◽  
Jinxia Xiang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Adenosine Diphosphate ◽  
Mitogen Activated Protein Kinase ◽  
Drought Tolerant ◽  
Mitogen Activated Protein ◽  
Phenylpropanoid Biosynthesis ◽  
Peg Treatment ◽  
Use Efficiency ◽  
Protein Kinase Signaling

Drought stress is a major environmental stress, which adversely affects the biological and molecular processes of plants, thereby impairing their growth and development. In the present study, we found that the expression level of OsTBP2.2 which encodes for a nucleus-localized protein member belonging to transcription factor IID (TFIID) family, was significantly induced by polyethylene glycol (PEG) treatment. Therefore, knockdown mutants of OsTBP2.2 gene were generated to investigate the role of OsTBP2.2 in rice response to drought stress. Under the condition of drought stress, the photosynthetic rate, transpiration rate, water use efficiency, and stomatal conductance were significantly reduced in ostbp2.2 lines compared with wild type, Dongjin (WT-DJ). Furthermore, the RNA-seq results showed that several main pathways involved in “MAPK (mitogen-activated protein kinase) signaling pathway”, “phenylpropanoid biosynthesis”, “defense response” and “ADP (adenosine diphosphate) binding” were altered significantly in ostbp2.2. We also found that OsPIP2;6, OsPAO and OsRCCR1 genes were down-regulated in ostbp2.2 compared with WT-DJ, which may be one of the reasons that inhibit photosynthesis. Our findings suggest that OsTBP2.2 may play a key role in rice growth and the regulation of photosynthesis under drought stress and it may possess high potential usefulness in molecular breeding of drought-tolerant rice.

scholarly journals Overexpression of the Trehalase Gene AtTRE1 Leads to Increased Drought Stress Tolerance in Arabidopsis and Is Involved in Abscisic Acid-Induced Stomatal Closure

2013 ◽  
Vol 161 (3) ◽  
pp. 1158-1171 ◽  
Author(s):  
Hilde Van Houtte ◽  
Lies Vandesteene ◽  
Lorena López-Galvis ◽  
Liesbeth Lemmens ◽  
Ewaut Kissel ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Stomatal Closure ◽  
Drought Stress Tolerance

scholarly journals F-Box Protein DOR Functions As a Novel Inhibitory Factor for Abscisic Acid-Induced Stomatal Closure under Drought Stress in Arabidopsis

2008 ◽  
Vol 148 (4) ◽  
pp. 2121-2133 ◽  
Author(s):  
Yu'e Zhang ◽  
Wenying Xu ◽  
Zhonghui Li ◽  
Xing Wang Deng ◽  
Weihua Wu ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Stomatal Closure ◽  
Inhibitory Factor ◽  
F Box Protein

scholarly journals Rice Calcium/Calmodulin-Dependent Protein Kinase Directly Phosphorylates a Mitogen-Activated Protein Kinase Kinase to Regulate Abscisic Acid Responses

2020 ◽  
Author(s):  
Min Chen ◽  
Lan Ni ◽  
Jing Chen ◽  
Manman Sun ◽  
Caihua Qin ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Protein Kinase ◽  
Mitogen Activated Protein Kinase ◽  
Activation Loop ◽  
Aba Signaling ◽  
Dependent Protein Kinase ◽  
N Terminus ◽  
Mitogen Activated Protein ◽  
Dependent Protein ◽  
Direct Target

ABSTRACTCa2+/calmodulin (CaM)-dependent protein kinase (CCaMK) is an important positive regulator of abscisic acid (ABA) and abiotic stress signaling in plants and is believed to act upstream of mitogen-activated protein kinase (MAPK) in ABA signaling. However, it is unclear how CCaMK activates MAPK in ABA signaling. Here, we show that OsDMI3, a rice (Oryza sativa) CCaMK, directly interacts with and phosphorylates OsMKK1, a MAPK kinase (MKK) in rice, in vitro and in vivo. OsDMI3 was found to directly phosphorylate Thr-25 in the N-terminus of OsMKK1, and this Thr-25 phosphorylation is OsDMI3-specific in ABA signaling. The activation of OsMKK1 and its downstream kinase OsMPK1 is dependent on Thr-25 phosphorylation of OsMKK1 in ABA signaling. Moreover, ABA treatment also induces the phosphorylation in the activation loop of OsMKK1, and the two phosphorylations in the N-terminus and in the activation loop are independent. Further analyses revealed that OsDMI3-mediated phosphorylation of OsMKK1 positively regulates ABA responses in seed germination, root growth, and tolerance to both water stress and oxidative stress. Our results indicate that OsMKK1 is a direct target of OsDMI3, and OsDMI3-mediated phosphorylation of OsMKK1 plays an important role in the activation of MAPK cascade and ABA signaling.One-sentence summaryOsMKK1 is a direct target of OsDMI3, and OsDMI3-mediated phosphorylation of OsMKK1 plays an important role in the activation of MAPK cascade and ABA signaling.The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Mingyi Jiang ([email protected])

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