scholarly journals Transcriptome Analysis of Chloris virgata, Which Shows the Fastest Germination and Growth in the Major Mongolian Grassland Plant

2021 ◽  
Vol 12 ◽  
Author(s):  
Byambajav Bolortuya ◽  
Shintaro Kawabata ◽  
Ayumi Yamagami ◽  
Bekh-Ochir Davaapurev ◽  
Fuminori Takahashi ◽  
...  

Plants in Mongolian grasslands are exposed to short, dry summers and long, cold winters. These plants should be prepared for fast germination and growth activity in response to the limited summer rainfall. The wild plant species adapted to the Mongolian grassland environment may allow us to explore useful genes, as a source of unique genetic codes for crop improvement. Here, we identified the Chloris virgata Dornogovi accession as the fastest germinating plant in major Mongolian grassland plants. It germinated just 5 h after treatment for germination initiation and showed rapid growth, especially in its early and young development stages. This indicates its high growth potential compared to grass crops such as rice and wheat. By assessing growth recovery after animal bite treatment (mimicked by cutting the leaves with scissors), we found that C. virgata could rapidly regenerate leaves after being damaged, suggesting high regeneration potential against grazing. To analyze the regulatory mechanism involved in the high growth potential of C. virgata, we performed RNA-seq-based transcriptome analysis and illustrated a comprehensive gene expression map of the species. Through de novo transcriptome assembly with the RNA-seq reads from whole organ samples of C. virgata at the germination stage (2 days after germination, DAG), early young development stage (8 DAG), young development stage (17 DAG), and adult development stage (28 DAG), we identified 21,589 unified transcripts (contigs) and found that 19,346 and 18,156 protein-coding transcripts were homologous to those in rice and Arabidopsis, respectively. The best-aligned sequences were annotated with gene ontology groups. When comparing the transcriptomes across developmental stages, we found an over-representation of genes involved in growth regulation in the early development stage in C. virgata. Plant development is tightly regulated by phytohormones such as brassinosteroids, gibberellic acid, abscisic acid, and strigolactones. Moreover, our transcriptome map demonstrated the expression profiles of orthologs involved in the biosynthesis of these phytohormones and their signaling networks. We discuss the possibility that C. virgata phytohormone signaling and biosynthesis genes regulate early germination and growth advantages. Comprehensive transcriptome information will provide a useful resource for gene discovery and facilitate a deeper understanding of the diversity of the regulatory systems that have evolved in C. virgata while adapting to severe environmental conditions.

2017 ◽  
Vol 63 (No. 1) ◽  
pp. 1-8 ◽  
Author(s):  
Cukor Jan ◽  
Baláš Martin ◽  
Kupka Ivo ◽  
Tužinský Marek

The paper presents an evaluation of the growth of newly established forest stands on former agricultural land and furthermore describes the state of the upper part of the soils in these stands in comparison with neighbouring grassland in the Orlické hory Mountains. The new Norway spruce stands show an extremely high growth potential, usually significantly higher in comparison with areas forested for more generations/rotations. The formation of the surface humus layer also showed fast progress, the amount of dry mass of soil organic matter reaching values almost typical of permanently forested sites. The soils of newly afforested lands tend to resemble the status of forest soil – there was observed a process of acidification and nutrient depletion, probably connected with accumulation of the tree biomass.


2020 ◽  
Vol 21 (3) ◽  
pp. 848
Author(s):  
Yuan Zhou ◽  
Di Zhao ◽  
Li Shuang ◽  
Dongxue Xiao ◽  
Yuanhu Xuan ◽  
...  

Meloidogyne incognita and Meloidogyne graminicola are root-knot nematodes (RKNs) infecting rice (Oryza sativa L.) roots and severely decreasing yield, whose mechanisms of action remain unclear. We investigated RKN invasion and development in rice roots through RNA-seq transcriptome analysis. The results showed that 952 and 647 genes were differently expressed after 6 (invasion stage) and 18 (development stage) days post inoculation, respectively. Gene annotation showed that the differentially expressed genes were classified into diverse metabolic and stress response categories. Furthermore, phytohormone, transcription factor, redox signaling, and defense response pathways were enriched upon RKN infection. RNA-seq validation using qRT-PCR confirmed that CBL-interacting protein kinase (CIPK) genes (CIPK5, 8, 9, 11, 14, 23, 24, and 31) as well as brassinosteroid (BR)-related genes (OsBAK1, OsBRI1, D2, and D11) were altered by RKN infection. Analysis of the CIPK9 mutant and overexpressor indicated that the RKN populations were smaller in cipk9 and larger in CIPK9 OX, while more galls were produced in CIPK9 OX plant roots than the in wild-type roots. Significantly fewer numbers of second-stage infective juveniles (J2s) were observed in the plants expressing the BR biosynthesis gene D2 mutant and the BR receptor BRI1 activation-tagged mutant (bri1-D), and fewer galls were observed in bri1-D roots than in wild-type roots. The roots of plants expressing the regulator of ethylene signaling ERS1 (ethylene response sensor 1) mutant contained higher numbers of J2s and developed more galls compared with wild-type roots, suggesting that these signals function in RKN invasion or development. Our findings broaden our understanding of rice responses to RKN invasion and provide useful information for further research on RKN defense mechanisms.


Chemosphere ◽  
2018 ◽  
Vol 196 ◽  
pp. 69-77 ◽  
Author(s):  
Luong Van Duc ◽  
Bongkeun Song ◽  
Hiroaki Ito ◽  
Takehide Hama ◽  
Masashi Otani ◽  
...  

1999 ◽  
Vol 256 (1) ◽  
pp. 184-191 ◽  
Author(s):  
Hiroshi Hino ◽  
Chise Tateno ◽  
Hajime Sato ◽  
Chihiro Yamasaki ◽  
Shigeru Katayama ◽  
...  

1984 ◽  
Vol 103 (1) ◽  
pp. 137-153 ◽  
Author(s):  
J. E. Frisch ◽  
T. E. Vercoe

SummaryCalves from three breeds, Brahman, Hereford × Shorthorn (HS) and Brahman × HS (BX), were divided equally into two groups, one of which was treated every 3 weeks from birth onwards to control ticks and gastrointestinal helminths, and one of which was untreated. Mortalities, growth rates and levels of resistance to environmental stresses that affected both mortality and growth under grazing conditions were recorded for all animals up to weaning (6 months) and for all males up to 15 months of age. The Brahmans were the most and the HS were the least resistant to environmental stresses, each of which was shown to depress growth in proportion to its magnitude and to contribute to the high mortalities of the HS. All breeds responded positively to parasite control with the greatest response in both survival and growth in the HS breed and the least response in the Brahman breed.Samples of males from the various breed-treatment groups were taken into pens where they were protected from environmental stresses and fed both low-quality pasture hay and high-quality lucerne hay ad libitum. Measurements were made of fasting metabolism, maintenance requirement, voluntary food intake and gain, variables related to the growth potential of each animal. The HS animals had the highest whilst the Brahmans had the lowest values for each variable.However, despite their low growth potential, the Brahmans had the highest growtli rate, and the HS, despite their high growth potential, had the lowest growth rate, when growth was measured in the presence of all environmental stresses. When parasites were controlled, growth rates were highest for the BX, the breed with intermediate growtli potential, and did not differ between the HS and Brahmans. These interactions arose because of the different contributions of resistance to environmental stresses and growth potential to growth rate measured at the different levels of environmental stresses. The relevance of these interactions to breed evaluation and cross-breeding is considered.Growth potential and resistance to environmental stresses were negatively correlated both between and within breeds, though the latter was biased by the effects of compensation. The influence of these relationships on the likely outcome of selection for increased growth rate, both between and within breeds, is discussed.


Author(s):  
Christoph Albert ◽  
Andrea Caggese

Abstract We analyze a multiyear, multicountry entrepreneurship survey with more than one million observations to identify startups with low and high growth potential. We confirm the validity of these ex ante measures with ex post firm-level information on employment growth. We find that negative aggregate financial shocks reduce all startup types, but their effect is significantly stronger for startups with high growth potential, especially when GDP growth is low. Our results uncover a new composition of entry channel that significantly reduces employment growth and is potentially important for explaining slow recoveries after financial crises.


Materials ◽  
2020 ◽  
Vol 13 (11) ◽  
pp. 2429
Author(s):  
Sebastian Micus ◽  
Michael Haupt ◽  
Götz T. Gresser

Experts attest the smart textiles market will have high growth potential during the next ten years. Laser soldering is considered to be a good contacting method because it is a contactless process. For this reason, it is intended to investigate the contacting process of printed circuit boards (PCB) to isolated conductive textile strips by means of a ytterbium-doped fiber laser (1064 nm). During the investigation, the copper strands in the textile tape were stripped by the laser and soldered to the PCB without any transport of the textile. Therefore, we investigated different sets of parameters by means of a design of experiment (DoE) for different types of solder pastes. Finally, the joinings were electrically analyzed using a contact resistance test, optically with a REM examination, and mechanically using a peeling test.


1970 ◽  
Vol 48 (4) ◽  
pp. 699-704 ◽  
Author(s):  
J. S. Yorke ◽  
G. R. Sagar

Development of the root system of peas grown on filter paper between vertical glass sheets was studied during the period 6 to 20 days after germination. Elongation of each whole root system proceeded at alternate high and low rates. At the end of each period of low growth rate, a higher order of roots emerged. Secondary roots tended to occur in clumps. Secondary roots with the greatest growth potential occurred midway within that region of the primary root from which secondary roots emerged, and also, but less markedly, at the positions of clumps. The timing and duration of elongation of roots with high growth potential differed from that of roots with lower growth.Growth potential of secondary root appears to be partly determined at the time of root initiation. Such a process would precondition a seedling to a particular environment.


2019 ◽  
Vol 16 (02) ◽  
pp. 1950020
Author(s):  
Michael Sheriff ◽  
Moreno Muffatto

University spin-offs with patents (USOPs) are entrepreneurial ventures created by exploiting research results and inventions. USOPs are characterized with a high growth potential and are capable of attracting external investments. To explore the challenges apparent in the creation of USOPs, we reviewed the existing framework to identify key missing elements. Our study proposes a new framework that incorporates Key elements that enhance the creation of USOPs. The new framework focused on the interactions of elements such as national intellectual property regimes, national university assessments, the local entrepreneurship ecosystems where the universities are located, the entrepreneurial orientations and strategies of universities, and stakeholders as independent variables with USOPs as the dependent variable. There is therefore a need for a critical reflection on national and university policies and strategies to enhance the creation of USOPs.


Sign in / Sign up

Export Citation Format

Share Document