Overexpression of NtSOS2 From Halophyte Plant N. tangutorum Enhances Tolerance to Salt Stress in Arabidopsis

The Salt Overly Sensitive (SOS) signaling pathway is key in responding to salt stress in plants. SOS2, a central factor in this pathway, has been studied in non-halophytes such as Arabidopsis and rice, but has so far not been reported in the halophyte Nitraria tangutorum. In order to better understand how Nitraria tangutorum acquires its tolerance for a high salt environment, here, the NtSOS2 was cloned from Nitraria tangutorum, phylogenetic analyses showed that NtSOS2 is homologous to the SOS2 of Arabidopsis and rice. Gene expression profile analysis showed that NtSOS2 localizes to the cytoplasm and cell membrane and it can be induced by salt stress. Transgenesis experiments showed that exogenous expression of NtSOS2 reduces leaf mortality and improves the germination rate, biomass and root growth of Arabidopsis under salt stress. Also, exogenous expression of NtSOS2 affected the expression of ion transporter-related genes and can rescue the phenotype of sos2-1 under salt stress. All these results revealed that NtSOS2 plays an important role in plant salt stress tolerance. Our findings will be of great significance to further understand the mechanism of salt tolerance and to develop and utilize molecular knowledge gained from halophytes to improve the ecological environment.

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Genome-Wide Identification and Expression Profile Analysis of the NF-Y Transcription Factor Gene Family in Petunia hybrida

Plants ◽

10.3390/plants9030336 ◽

2020 ◽

Vol 9 (3) ◽

pp. 336 ◽

Cited By ~ 1

Author(s):

Qian Wei ◽

Shiyun Wen ◽

Chuying Lan ◽

Yixun Yu ◽

Guoju Chen

Keyword(s):

Stress Response ◽

Gene Family ◽

Stress Responses ◽

Petunia Hybrida ◽

Profile Analysis ◽

Phylogenetic Analyses ◽

Expression Patterns ◽

Draft Genome ◽

Rna Seq ◽

Expression Profile Analysis

Nuclear Factor Ys (NF-Ys) are a class of heterotrimeric transcription factors that play key roles in many biological processes, such as abiotic stress responses, flowering time, and root development. The petunia (Petunia hybrida) is a model ornamental plant, and its draft genome has been published. However, no details regarding the NF-Y gene family in petunias are available. Here, 27 NF-Y members from the petunia genome were identified, including 10 PhNF-YAs, 13 PhNF-YBs, and 4 PhNF-YCs. Multiple alignments showed that all PhNF-Y proteins had clear conserved core regions flanked by non-conserved sequences. Phylogenetic analyses identified five pairs of orthologues NF-YB proteins from Petunia and Arabidopsis, and six pairs of paralogues NF-Y proteins in Petunia. Analysis of the gene structure and conserved motifs further confirmed the closer relationship in each subfamily. Bioinformatics analysis revealed that 16 PhNF-Ys could be targeted by 18 miRNA families. RNA-seq results showed that expression patterns of PhNF-Ys among four major organs (leaf, stem, flower, and root) were clustered into six major groups. The stress response pattern of PhNF-Ys was identified under cold, heat, drought, and salinity treatments. Based on the RNA-seq data, we found that 3 genes responded to drought, 4 genes responded to salt, 10 genes responded to cold, and 9 genes responded to hot. In conclusion, this study provides useful information for further studying the functions of NF-Ys in stress response.

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Whole genomic expression profile analysis of early and advanced staged colorectal cancer biopsy samples: searching for candidate pathways involved in the carcinogenesis and pathomechanism

Zeitschrift für Gastroenterologie ◽

10.1055/s-2005-869731 ◽

2005 ◽

Vol 43 (05) ◽

Author(s):

B Molnár ◽

O Galamb ◽

F Sipos ◽

B Győrffy ◽

S Spisák ◽

...

Keyword(s):

Colorectal Cancer ◽

Expression Profile ◽

Profile Analysis ◽

Genomic Expression ◽

Expression Profile Analysis ◽

Cancer Biopsy

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Salinity Tolerance of Black Gram Cultivars During Germination and Early Seedling Growth

Cercetari Agronomice in Moldova ◽

10.2478/cerce-2018-0025 ◽

2018 ◽

Vol 51 (3) ◽

pp. 51-68 ◽

Cited By ~ 1

Author(s):

M.K. Hasan ◽

M.S. Islam ◽

M.R. Islam ◽

H.N. Ismaan ◽

A. El Sabagh

Keyword(s):

Salt Stress ◽

Salinity Tolerance ◽

Seedling Growth ◽

Negative Impact ◽

Germination Rate ◽

Dry Weight ◽

Germination Percentage ◽

Shoot Length ◽

Sand Culture ◽

Black Gram

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.

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