scholarly journals A Novel Banana Mutant “RF 1” (Musa spp. ABB, Pisang Awak Subgroup) for Improved Agronomic Traits and Enhanced Cold Tolerance and Disease Resistance

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaoyi Wang ◽  
Anbang Wang ◽  
Yujia Li ◽  
Yi Xu ◽  
Qing Wei ◽  
...  

Banana is a major fruit crop grown in tropical and subtropical regions worldwide. Among cultivars, “FenJiao, FJ” (Musa spp. ABB, Pisang Awak subgroup) is a popular variety of bananas, due to its better sugar-acid blend and relatively small fruit shape. However, because the traditional FJ variety grows relatively high in height, it is vulnerable to lodging and unsuitable for harvesting. In this study, we sought desirable banana mutants by carrying out ethyl methanesulfonate (EMS) mutagenesis with the FJ cultivar. After the FJ shoot tips had been treated with 0.8% (v/v) EMS for 4 h, we obtained a stably inherited mutant, here called “ReFen 1” (RF1), and also observed a semi-dwarfing phenotype. Compared with the wild type (FJ), this RF1 mutant featured consistently improved agronomic traits during 5-year field experiments conducted in three distinct locations in China. Notably, the RF1 plants showed significantly enhanced cold tolerance and Sigatoka disease resistance, mainly due to a substantially increased soluble content of sugar and greater starch accumulation along with reduced cellulose deposition. Therefore, this study not only demonstrated how a powerful genetic strategy can be used in fruit crop breeding but also provided insight into the identification of novel genes for agronomic trait improvement in bananas and beyond.

HortScience ◽  
2005 ◽  
Vol 40 (1) ◽  
pp. 43-46 ◽  
Author(s):  
Lingxia Zhao ◽  
Chengxiang Qiu ◽  
Jingfu Li ◽  
Yourong Chai ◽  
Guoyin Kai ◽  
...  

Solanum lycopersicoides is a valuable genetic resource for tomato (Lycopersicon esculentum) genetic improvement. However, there are few reports on its agronomic traits such as disease resistance and cold tolerance. In this paper, the resistance to cucumber mosaic virus (CMV) and leaf mold (Cladosporium fulvum Cooke) and cold tolerance of five lines of S. lycopersicoides were studied through investigation of disease inoculation and electrolyte leakage analysis. The results showed that S. lycopersicoides was highly resistant or immune to CMV and leaf mold and more tolerant to low temperature than L. esculentum. This study is helpful for the genetic improvement of tomato by using S. lycopersicoides as breeding materials.


Author(s):  
Trương Thị Hồng Hải ◽  
Nguyễn Thị Diệu Thể ◽  
Phan Thu Thảo

In order to establish the pure line of sponge gourd containing aroma feature, we selected the desirable inbred lines by using a self-pollinating method. The present study was investigated to estimate the morphological traits and fruit quality of 6 sponge gourd inbred lines which generated at 4th generation of an aroma Luffa accession B29 under plastic house conditions. The experiment was conducted in a randomized complete block design (RCBD) with three replications, from May to November in 2016. Five plants per replication were examined. The results indicated that all inbred lines could grow well under plastic house conditions. The inbred lines had the same stem and leaf traits; whereas fruit shape, skin color and fruit veins color were observed differently among inbred lines. The aromatic trait was retained in all inbred lines either before or after cooking. The high yield was found in lines BC1 and BC2 by 10.1 tons/ha and 10.7 tons/ha, respectively. These inbred lines should be examined in open field condition to confirm the presence of aromatic trait and yield potential before completion of the procedures for recognition of new Luffa varieties.  


Plant Disease ◽  
2011 ◽  
Vol 95 (3) ◽  
pp. 263-268 ◽  
Author(s):  
S. K. Gremillion ◽  
A. K. Culbreath ◽  
D. W. Gorbet ◽  
B. G. Mullinix ◽  
R. N. Pittman ◽  
...  

Field experiments were conducted in 2002 to 2006 to characterize yield potential and disease resistance in the Bolivian landrace peanut (Arachis hypogaea) cv. Bayo Grande, and breeding lines developed from crosses of Bayo Grande and U.S. cv. Florida MDR-98. Diseases of interest included early leaf spot, caused by the fungus Cercospora arachidicola, and late leaf spot, caused by the fungus Cercosporidium personatum. Bayo Grande, MDR-98, and three breeding lines, along with U.S. cvs. C-99R and Georgia Green, were included in split-plot field experiments in six locations across the United States and Bolivia. Whole-plot treatments consisted of two tebuconazole applications and a nontreated control. Genotypes were the subplot treatments. Area under the disease progress curve (AUDPC) for percent defoliation due to leaf spot was lower for Bayo Grande and all breeding lines than for Georgia Green at all U.S. locations across years. AUDPC for disease incidence from one U.S. location indicated similar results. Severity of leaf spot epidemics and relative effects of the genotypes were less consistent in the Bolivian experiments. In Bolivia, there were no indications of greater levels of disease resistance in any of the breeding lines than in Bayo Grande. In the United States, yields of Bayo Grande and the breeding lines were greater than those of the other genotypes in 1 of 2 years. In Bolivia, low disease intensity resulted in the highest yields in Georgia Green, while high disease intensity resulted in comparable yields among the breeding lines, MDR-98, and C-99R. Leaf spot suppression by tebuconazole was greater in Bolivia than in the United States. This result indicates a possible higher level of fungicide resistance in the U.S. population of leaf spot pathogens. Overall, data from this study suggest that Bayo Grande and the breeding lines may be desirable germplasm for U.S. and Bolivian breeding programs or production.


Author(s):  
Thien Minh Nguyen ◽  
Tien Thi My Pham

The agronomic values of this population have been evaluated in the field experiments based on their phenotypic performance of agronomic traits, but the genetic variability of this population needs to be evaluated via techniques based on genetic material - DNA. In this study, the genetic variability in the investigated population of 71 hybrids and their parents was evaluated by RAPD technique, using eight selected arbitrarily primers; Genetic parameters and dendrogram expressing the genetic relationships among the investigated population were analyzed by GenALEx 6.1, Popgene 1.31 and NTSYSpc 2.1 softwares. Eight primers were used to generate the amplify products on each individual in the investigated population. From 74 genotypes, a total of 109 fragments were generated, among which, there were 89 polymorphic bands representing 81.65% with an average of 11 polymorphic bands/primer. Genetic similarity coefficient among the investigated population, based on DICE coefficient, ranged from 0.560 (LH05/0822 and PB260) to 0.991 (LH05/0781 and LH05/0841) with an average of 0,796, meaning that the genetic distance among ranged from 0.009 to 0.440 with an average of 0.231. The Shannon index and mean heterozygosity values were 0.328 and 0,176, respectively. This indicated that the progenies of the two investigated crosses possessed a relatively high range of genetic variability. The analysis of molecular variance (AMOVA) showed that genetic variation within population represented 62%, while genetic variation among two different crosses contributes 38% to the total genetic variability. Dendrogram based on DICE’s genetic similarity using UPGMA method showed that the hybrids divide into two major genetic groups (0.75), but the crosses were scattered independently of the hybrid.


Author(s):  
E. N. Sedov ◽  
S. A. Korneyeva ◽  
T. V. Yanchuk ◽  
Z. M. Serova

A careful 63-year-old work on apple breeding, resulting in the creation of 54 its varieties, included in the State Register of breeding achievements admitted for use, makes it possible to speak about the ways of reduction in the time of the actual breeding process and the subsequent stages of accelerating the introduction of new varieties into production. According to the experience of the Russian Research Institute of Fruit Crop Breeding, an average of 19 years is spent from hybridization to the adoption of a variety for State trials, and from hybridization to inclusion in the State Register it is spent 27 years. The requirements for new apple varieties are changing and becoming tougher to a large extent during this period. We off er the possibility of reducing the time to create a new variety by combining in time the actual breeding process and the primary variety study. For this purpose already during the selection and transfer of biannual seedlings to the breeding orchard it is necessary to multiply and include the best seedlings on morphological features with a culture of 5 points (no more than 1 seedling per thousand selected ones) in the primary study. In this case the actual breeding process is combined with the primary study. When transferring the variety to the state trial, it is necessary to lay orchards of a small production testing of 20-40 trees of a new variety in each of the three replications. The time of single breeders is a thing of the past. To create varieties that meet high requirements of production (convenient for cultivation, highly adapted to local conditions, with a certain shape of tree crowns, with the fruits of high commodity and consumer qualities), interdisciplinary teams are needed, which include not only breeders, but also pomologists, geneticists, cytoembryologists, biochemists, physiologists, phytopathologists, and agricultural technicians. Usually years, and sometimes decades are spent on the creation of well-coordinated teams of professionals of diff erent specialties, but only such teams are able to create varieties that meet all main requirements. At Russian Research Institute of Fruit Crop Breeding such a team consists of 22 people.


Agronomy ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 349
Author(s):  
Manuel Guzman ◽  
Luis Vilain ◽  
Tatiana Rondon ◽  
Juan Sanchez

Evaluation of sowing density is an important factor for achieving maximum yields without affecting other agronomic traits. Field experiments were conducted during three consecutive years (2008, 2009 and 2010) to determinate the effect of four sowing density (62,500; 83,333; 100,000 and 142,857 pl ha−1) on yields and its components of two cotton varieties, ‘Delta Pine 16′ and ‘SN-290′ in Venezuela. The traits evaluated were lint yield, boll weight, number of seeds per boll, 100-seed weight, and fiber content. Highly significant differences (p ≤ 0.01) were observed among genotypes, sowing density and their interactions for all traits. Sowing density was not affected by year factor. High lint yield was found in ‘SN-290′ (4216.2 kg ha−1) at 100,000 pl ha−1; and in ‘Delta Pine 16′ (3917.3 kg ha−1) at 83,333 pl ha−1. The highest sowing density (142,857 pl ha−1), decrease lint yield and yield components in the genotypes. The highest boll weight was obtained by ‘SN-290′ with 6.4 g in average. All sowing densities evaluated resulted in lint percentages above 40%. Cotton lint yield was positively correlated with all yield components. Our results indicate that highest lint yields could be obtained with sowing densities between 83,333 and 100,000 pl ha−1 depending upon varieties used across savannahs of Venezuela.


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