scholarly journals Drought Tolerant Near Isogenic Lines of Pusa 44 Pyramided With qDTY2.1 and qDTY3.1, Show Accelerated Recovery Response in a High Throughput Phenomics Based Phenotyping

2022 ◽  
Vol 12 ◽  
Author(s):  
Priyanka Dwivedi ◽  
Naleeni Ramawat ◽  
Dhandapani Raju ◽  
Gaurav Dhawan ◽  
S. Gopala Krishnan ◽  
...  
Keyword(s):  
Drought Stress ◽  
High Throughput ◽  
Near Infrared ◽  
Early Stage ◽  
Strong Association ◽  
Tolerance Index ◽  
Near Isogenic Lines ◽  
Isogenic Lines ◽  
Drought Tolerant ◽  
Stress Susceptibility

Reproductive stage drought stress (RSDS) is a major challenge in rice production worldwide. Cultivar development with drought tolerance has been slow due to the lack of precise high throughput phenotyping tools to quantify drought stress-induced effects. Most of the available techniques are based on destructive sampling and do not assess the progress of the plant’s response to drought. In this study, we have used state-of-the-art image-based phenotyping in a phenomics platform that offers a controlled environment, non-invasive phenotyping, high accuracy, speed, and continuity. In rice, several quantitative trait loci (QTLs) which govern grain yield under drought determine RSDS tolerance. Among these, qDTY2.1 and qDTY3.1 were used for marker-assisted breeding. A set of 35 near-isogenic lines (NILs), introgressed with these QTLs in the popular variety, Pusa 44 were used to assess the efficiency of image-based phenotyping for RSDS tolerance. NILs offered the most reliable contrast since they differed from Pusa 44 only for the QTLs. Four traits, namely, the projected shoot area (PSA), water use (WU), transpiration rate (TR), and red-green-blue (RGB) and near-infrared (NIR) values were used. Differential temporal responses could be seen under drought, but not under unstressed conditions. NILs showed significant level of RSDS tolerance as compared to Pusa 44. Among the traits, PSA showed strong association with yield (80%) as well as with two drought tolerances indices, stress susceptibility index (SSI) and tolerance index (TOL), establishing its ability in identifying the best drought tolerant NILs. The results revealed that the introgression of QTLs helped minimize the mean WU per unit of biomass per day, suggesting the potential role of these QTLs in improving WU-efficiency (WUE). We identified 11 NILs based on phenomics traits as well as performance under imposed drought in the field. The study emphasizes the use of phenomics traits as selection criteria for RSDS tolerance at an early stage, and is the first report of using phenomics parameters in RSDS selection in rice.

scholarly journals Drought Tolerant near Isogenic Lines (NILs) of Pusa 44 Developed through Marker Assisted Introgression of qDTY2.1 and qDTY3.1 Enhances Yield under Reproductive Stage Drought Stress

Agriculture ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 64
Author(s):  
Priyanka Dwivedi ◽  
Naleeni Ramawat ◽  
Gaurav Dhawan ◽  
Subbaiyan Gopala Krishnan ◽  
Kunnummal Kurungara Vinod ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Grain Quality ◽  
Reproductive Stage ◽  
Recurrent Parent ◽  
Near Isogenic Lines ◽  
Improvement Project ◽  
Isogenic Lines ◽  
Drought Tolerant ◽  
Rice Improvement

Reproductive stage drought stress (RSDS) is detrimental for rice, which affects its productivity as well as grain quality. In the present study, we introgressed two major quantitative trait loci (QTLs), namely, qDTY2.1 and qDTY3.1, governing RSDS tolerance in a popular high yielding non-aromatic rice cultivar, Pusa 44, through marker-assisted backcross breeding (MABB). Pusa 44 is highly sensitive to RSDS, which restricts its cultivation across drought-prone environments. Foreground selection was carried out using markers, RM520 for qDTY3.1 and RM 521 for qDTY2.1. Background selection was achieved with 97 polymorphic SSR markers in tandem with phenotypic selection to achieve faster recurrent parent genome (RPG) recovery. Three successive backcrosses followed by three selfings aided RPG recoveries of 98.6% to 99.4% among 31 near isogenic lines (NILs). Fourteen NILs were found to be significantly superior in yield and grain quality under RSDS with higher drought tolerance efficiency (DTE) than Pusa 44. Among these, the evaluation of two promising NILs in the multilocational trial during Kharif 2019 showed that they were significantly superior to Pusa 44 under reproductive stage drought stress, while performing on par with Pusa 44 under normal irrigated conditions. These di-QTL pyramided drought-tolerant NILs are in the final stages of testing the All India Coordinated Rice Improvement Project varietal trials for cultivar release. Alternately, the elite drought-tolerant Pusa 44 NILs will serve as an invaluable source of drought tolerance in rice improvement.

Selection indices to identify maize (Zea mays L.) hybrids adapted under drought-stress and drought-free conditions in a tropical climate

2016 ◽  
Vol 67 (10) ◽  
pp. 1087 ◽  
Author(s):  
Bhupender Kumar ◽  
Satish Kumar Guleria ◽  
Subhash M. Khanorkar ◽  
Rajender Babu Dubey ◽  
Jashvantlal Patel ◽  
...  
Keyword(s):  
Zea Mays ◽  
Drought Stress ◽  
Grain Yield ◽  
Zea Mays L ◽  
Tolerance Index ◽  
Yield Performance ◽  
Susceptibility Index ◽  
Drought Tolerant ◽  
Selection Indices ◽  
Stress Susceptibility

Drought stress is the most important production constraint in maize (Zea mays L.), especially in rainfed agriculture. To improve productivity of rainfed maize, the development of hybrids with tolerance to drought stress is an important objective in maize breeding programs. The present study was undertaken to identify maize hybrids that perform better under drought-stress and drought-free conditions by using various selection indices. These selection indices were calculated on the basis of yield (t ha–1) performance of hybrids measured under drought stress and optimum environments. A set of 38 cultivars was evaluated at 10 environments (representing five each of drought stress and optimum growing conditions). The average reduction in grain yield due to drought stress was 52%. Effects of genotype, environment and their interaction were significant sources of variation in determining grain yield, respectively explaining 5.0–7.4%, 55.0–60.2% and 12.0–15.0% of total variation in yield under drought-stress and drought-free conditions. Of eight selection indices considered for study, three indices such as harmonic mean, geometric mean, and stress tolerance index were identified as suitable for selection of genotypes capable of performing well both under drought-stress and drought-free environments. Drought response index and drought resistance index were found useful in identifying hybrids that performed better under drought stress. Stress susceptibility index was negatively correlated with yield measured under drought stress. Stress susceptibility index could be used as selection index but only in combination with yield performance data under water-deficit conditions in order to identify drought-tolerant hybrids with reasonable productivity. Test weight, shelling percentage, days to maturity, and ear girth were found to be useful traits for improving yield performance across diverse environments. Cultivation of identified drought-tolerant hybrids would be useful to enhance maize productivity in drought-stress environments.

scholarly journals Drought Resistance Indices for Screening of Potato (Solanum tuberosum L.) Genotypes

2019 ◽  
Vol 7 (8) ◽  
pp. 1118
Author(s):  
Zerihun Kebede Beyene ◽  
Firew Mekbib ◽  
Tesfaye Abebe ◽  
Asrat Asfaw
Keyword(s):  
Stress Tolerance ◽  
Solanum Tuberosum L ◽  
Geometric Mean ◽  
Strong Association ◽  
Principal Component ◽  
Screening Tools ◽  
Tolerance Index ◽  
Lattice Design ◽  
Terminal Drought ◽  
Drought Tolerant

In breeding for drought tolerance, availability of precise, cheap and easy to apply selection tool is critical. The aim of the present study was to identify potential screening tools that are useful for selection of drought tolerant genotypes in potato and select drought resistant potato genotypes. The study assessed sixty clones arranged in a 10×6 alpha lattice design with two replicates in a managed stress experiment. Two irrigation treatments were applied: fully watered non-stress and terminal drought, where the irrigation water supply to the crop was withheld after 50 % flowering to induce post-flowering stress. Stress indices were calculated based on tuber yield of genotypes in both stressed and non-stressed conditions. Identification of drought tolerant genotypes based on a single index was less informative as different indices identified different genotypes as drought tolerant. Hence, to determine the most desirable drought tolerant clones rank sum of indices, correlation, and bi-plot display of the principal component analysis was employed. The indices modified stress tolerance index based on non-stressed yield, Men productivity, Geometric mean productivity, Stress tolerance index, Harmonic mean, modified stress tolerance index based on stressed yield and Yield index exhibited strong association with both yield under stressed and non-stressed yield. These indices discriminated drought tolerant genotypes with higher tuber yielding potential both under stress and non-stress conditions. Genotypes CIP-398180.612, CIP-397069.5, and CIP-304371.67 were identified as drought tolerant. These genotypes could be potentially grown both under drought prone and potential environments and these selection attributes could help to develop climate resilient potato varieties.

scholarly journals Effective selection criteria for screening drought tolerant recombinant inbred lines of sunflower

Genetika ◽  
2013 ◽  
Vol 45 (1) ◽  
pp. 153-166 ◽  
Author(s):  
Nishtman Abdi ◽  
Reza Darvishzadeh ◽  
Hatami Maleki
Keyword(s):  
Inbred Line ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Geometric Mean ◽  
Inbred Lines ◽  
Harmonic Mean ◽  
Tolerance Index ◽  
Yield Performance ◽  
Drought Tolerant ◽  
Stress Susceptibility

In this study, seventy two sunflower recombinant inbred lines were tested for their yielding ability under both water-stressed and well-watered states. The inbred lines were evaluated in a rectangular 8?9 lattice design with two replications in both well-watered and water-stressed conditions, separately. Eight drought tolerance indices including stability tolerance index (STI), mean productivity (MP), geometric mean productivity (GMP), harmonic mean (HM), stress susceptibility index (SSI), tolerance index (TOL), yield index (YI) and yield stability index (YSI) were calculated based on grain yield for every genotype. Results showed the highest values of mean productivity (MP) index, geometric mean productivity (GMP), yield index (YI), harmonic mean (HM) and stress tolerance index (STI) indices for ?C134a? inbred line and least values of stress susceptibility index (SSI) and tolerance (TOL) for C61 inbred line. According to correlation of indices with yield performance under both drought stress and non-stress states and principle component analysis, indices including HM, MP, GMP and STI could properly distinguish drought tolerant sunflower inbred lines with high yield performance under both states. Cluster analysis of inbred lines using Ys, Yp and eight indices, categorized them into four groups including 19, 6, 26 and 19 inbred lines.

scholarly journals Physiological Screening for Drought Tolerance Traits in Vegetable Amaranth (Amaranthus tricolor) Germplasm

Agriculture ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 994
Author(s):  
Norain Jamalluddin ◽  
Festo J. Massawe ◽  
Sean Mayes ◽  
Wai Kuan Ho ◽  
Ajit Singh ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Geometric Mean ◽  
Tolerance Index ◽  
Genotypic Differences ◽  
Amaranthus Tricolor ◽  
Drought Tolerant ◽  
Drought Tolerance Indices ◽  
Tolerance Indices ◽  
Screening Trials

Amaranth (Amaranthus tricolor), an underutilized climate smart crop, is highly nutritious and possesses diverse drought tolerance traits, making it an ideal crop to thrive in a rapidly changing climate. Despite considerable studies on the growth and physiology of plants subjected to drought stress, a precise trait phenotyping strategy for drought tolerance in vegetable amaranth is still not well documented. In this study, two drought screening trials were carried out on 44 A. tricolor accessions in order to identify potential drought-tolerant A. tricolor germplasm and to discern their physiological responses to drought stress. The findings revealed that a change in stem biomass was most likely the main mechanism of drought adaptation for stress recovery, and dark-adapted quantum yield (Fv/Fm) could be a useful parameter for identifying drought tolerance in amaranth. Three drought tolerance indices: geometric mean productivity (GMP), mean productivity (MP) and stress tolerance index (STI) identified eight drought-tolerant accessions with stable performance across the two screening trials. The highly significant genotypic differences observed in several physiological traits among the amaranth accessions indicate that the amaranth panel used in this study could be a rich source of genetic diversity for breeding purposes for drought tolerance traits.

scholarly journals Response of potato (Solanum tuberosum L.) cultivars to drought stress under in vitro and field conditions

2022 ◽  
Vol 9 (1) ◽  
Author(s):  
Haitham E. M. Zaki ◽  
Khlode S. A. Radwan
Keyword(s):  
Solanum Tuberosum ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Tuber Yield ◽  
Potato Cultivars ◽  
Tolerance Index ◽  
Drought Tolerant ◽  
Middle East Countries ◽  
Wide Range

Abstract Background Potato (Solanum tuberosum L.), the world’s third most important crop, is frequently thought to be sensitive to moderately sensitive to drought, and yield has fallen considerably over consecutive stress periods. Drought produces a wide range of responses in potato, from physiological alterations to variations in growth rates and yield. Knowledge about these responses is essential for getting a full understanding of drought-tolerance mechanism in potato plants which will help in the identification of drought-tolerant cultivars. Results A set of 21 commercial potato cultivars representing the genetic diversity in the Middle East countries market were screened for drought tolerance by measuring morpho-physiological traits and tuber production under in vitro and field trials. Cultivars were exposed to drought stress ranging from no drought to 0.1, 0.2 and 0.3 mol L−1 sorbitol in in vitro-based screening and 60, 40 and 20% soil moisture content in field-based screening. Drought stress adversely affected plant growth, yield and cultivars differed for their responses. Shoots and roots fresh weights, root length, surface area of root, no. of roots, no. of leaves, leaf area, plant water content %, K+ content, under in vitro drought treatments and shoots fresh and dry weights, no. of tubers and tuber yield under field drought treatments were examined and all decreased due to drought. The stress tolerance index decreased with increasing drought in examined cultivars; nevertheless, it revealed a degree of tolerance in some of them. Grouping cultivars by cluster analysis for response to drought resulted in: (i) a tolerant group of five cultivars, (ii) a moderately tolerant group of 11 cultivars, and (iii) a sensitive group of five cultivars. Furthermore, stress-related genes, i.e., DRO, ERECTA, ERF, DREB and StMYB were up-regulated in the five cultivars of the tolerant group. Likewise, the stomatal conductance and transpiration explained high correlation with the tuber yield in this group of cultivars. Conclusion The diversity in germplasm indicated that potato cultivars can be developed for production under certain degrees of drought. Some cultivars are good candidates to be included in drought-tolerant breeding programs and recommended for cultivation in drought-stricken regions. Graphical Abstract

scholarly journals Evaluation of Drought Tolerance Indices for Screening Some of Corn (Zea mays L.) Cultivars under Environmental Conditions

2013 ◽  
Vol 5 (3) ◽  
pp. 388-393 ◽  
Author(s):  
Mohhamad Reza NAGHAVI ◽  
Alireza POUR ABOUGHADAREH ◽  
Marouf KHALILI
Keyword(s):  
Zea Mays ◽  
Drought Tolerance ◽  
Stress Tolerance ◽  
Zea Mays L ◽  
Stress Conditions ◽  
Tolerance Index ◽  
Drought Tolerant ◽  
Drought Tolerance Indices ◽  
Stress Susceptibility ◽  
Tolerance Indices

In order to study the effect of drought stress on eight cultivars corn (Zea mays L.), an experiment was conducted in a factorial experimental on the basis of randomized complete block design under two irrigated conditions during 2010-2011 cropping season. Twelve drought tolerance/resistance indices including stress tolerance index (STI), stress susceptibility index (SSI), tolerance index (TOL), geometric mean production (GMP), mean production (MP), yield index (YI), yield stability index (YSI), drought resistance index (DI), relative drought index (RDI), stress susceptibility percentage index (SSPI) and modified stress tolerance (K1STI and K2STI) were calculated based on grain yield under drought and irrigated conditions. Yield in stress and non-stress conditions were significantly and positively correlated with STI, GMP, MP, YI, TOL, DI, RDI, YSI, SSPI, K1STI, and K2STI and negatively correlated with SSI. Yield in stress and non-stress conditions were significantly and positively correlated with STI, GMP, MP, YI, TOL, DI, RDI, YSI, SSPI, K1STI, and K2STI and negatively correlated with SSI. Screening drought tolerant cultivars using ranking method, three dimensional plots discriminated cultivars ‘KSC720’, KSC 710GT and ‘KSC 700’ as the most drought tolerant. Cluster analysis classified the cultivars into three groups i.e., tolerant, susceptible and semi-susceptible to drought conditions. In general, Results of this study showed that among drought tolerance indices STI, YI, SSPI, K1STI, and K2STI can be used as the most suitable indicators for screening drought tolerant cultivars and ‘KSC720’, KSC 710 GT and ‘KSC 700’ had the highest tolerance to drought in our studies condition.

scholarly journals Estimation of drought tolerance among maize landraces from mini-core collection

Genetika ◽  
2014 ◽  
Vol 46 (3) ◽  
pp. 775-788 ◽  
Author(s):  
Violeta Andjelkovic ◽  
Natalija Kravic ◽  
Vojka Babic ◽  
Dragana Ignjatovic-Micic ◽  
Zoran Dumanovic ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Grain Yield ◽  
Stress Tolerance ◽  
Core Collection ◽  
High Density ◽  
Tolerance Index ◽  
Mini Core Collection ◽  
Drought Tolerant ◽  
Maize Landraces

Global climate change, its impact on stable food production in the future and possibilities to overcome the problem are the major priorities for research. Breeding varieties with increase adaptability to changing environments, together with better tolerance/resistance to abiotic stress, pest and diseases are possible solution. Maize is one of the most important crops, with high grain yield reduction induced by drought stress. In the present study twenty-six maize landraces from drought tolerant mini-core collection were tested under optimal, drought, and a combination of drought and high density stresses in the field. Morphological traits, plant height, total number of leaves, leaf length, leaf width, anthesis-silking interval and grain yield were recorded for each entry in two replications in three experiments. Besides, drought tolerant indices were evaluated to test the ability to separate more drought tolerant accessions from those with less stress tolerance. Five stress tolerance indices, including stress tolerance index (STI), mean productivity (MP), geometric mean productivity (GMP), stress susceptibility (SSI), and stress tolerance (TOL) were calculated. Data analyses revealed that STI, MP and GMP had positive and significant correlations with grain yield under all conditions. Three-dimensional diagrams displayed assignment of landraces L25, L1, L14, L3, L26, L15 and L16 to group A, based on the stress tolerance index and achieved grain yield under optimal, drought stress, and a combination of drought and high density stress. A biplot analysis efficiently separated groups of landraces with different level of drought tolerance and grain yield. Based on all obtained results, maize landraces L25, L14, L1 and L3, as the most valuable source of drought tolerance, could be recommended for further use in breeding programs.

Sign in / Sign up

Export Citation Format

Share Document