Response of potato (Solanum tuberosum L.) cultivars to drought stress under in vitro and field conditions

Abstract Background Potato (Solanum tuberosum L.), the world’s third most important crop, is frequently thought to be sensitive to moderately sensitive to drought, and yield has fallen considerably over consecutive stress periods. Drought produces a wide range of responses in potato, from physiological alterations to variations in growth rates and yield. Knowledge about these responses is essential for getting a full understanding of drought-tolerance mechanism in potato plants which will help in the identification of drought-tolerant cultivars. Results A set of 21 commercial potato cultivars representing the genetic diversity in the Middle East countries market were screened for drought tolerance by measuring morpho-physiological traits and tuber production under in vitro and field trials. Cultivars were exposed to drought stress ranging from no drought to 0.1, 0.2 and 0.3 mol L−1 sorbitol in in vitro-based screening and 60, 40 and 20% soil moisture content in field-based screening. Drought stress adversely affected plant growth, yield and cultivars differed for their responses. Shoots and roots fresh weights, root length, surface area of root, no. of roots, no. of leaves, leaf area, plant water content %, K+ content, under in vitro drought treatments and shoots fresh and dry weights, no. of tubers and tuber yield under field drought treatments were examined and all decreased due to drought. The stress tolerance index decreased with increasing drought in examined cultivars; nevertheless, it revealed a degree of tolerance in some of them. Grouping cultivars by cluster analysis for response to drought resulted in: (i) a tolerant group of five cultivars, (ii) a moderately tolerant group of 11 cultivars, and (iii) a sensitive group of five cultivars. Furthermore, stress-related genes, i.e., DRO, ERECTA, ERF, DREB and StMYB were up-regulated in the five cultivars of the tolerant group. Likewise, the stomatal conductance and transpiration explained high correlation with the tuber yield in this group of cultivars. Conclusion The diversity in germplasm indicated that potato cultivars can be developed for production under certain degrees of drought. Some cultivars are good candidates to be included in drought-tolerant breeding programs and recommended for cultivation in drought-stricken regions. Graphical Abstract

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Physiological Screening for Drought Tolerance Traits in Vegetable Amaranth (Amaranthus tricolor) Germplasm

Agriculture ◽

10.3390/agriculture11100994 ◽

2021 ◽

Vol 11 (10) ◽

pp. 994

Author(s):

Norain Jamalluddin ◽

Festo J. Massawe ◽

Sean Mayes ◽

Wai Kuan Ho ◽

Ajit Singh ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Geometric Mean ◽

Tolerance Index ◽

Genotypic Differences ◽

Amaranthus Tricolor ◽

Drought Tolerant ◽

Drought Tolerance Indices ◽

Tolerance Indices ◽

Screening Trials

Amaranth (Amaranthus tricolor), an underutilized climate smart crop, is highly nutritious and possesses diverse drought tolerance traits, making it an ideal crop to thrive in a rapidly changing climate. Despite considerable studies on the growth and physiology of plants subjected to drought stress, a precise trait phenotyping strategy for drought tolerance in vegetable amaranth is still not well documented. In this study, two drought screening trials were carried out on 44 A. tricolor accessions in order to identify potential drought-tolerant A. tricolor germplasm and to discern their physiological responses to drought stress. The findings revealed that a change in stem biomass was most likely the main mechanism of drought adaptation for stress recovery, and dark-adapted quantum yield (Fv/Fm) could be a useful parameter for identifying drought tolerance in amaranth. Three drought tolerance indices: geometric mean productivity (GMP), mean productivity (MP) and stress tolerance index (STI) identified eight drought-tolerant accessions with stable performance across the two screening trials. The highly significant genotypic differences observed in several physiological traits among the amaranth accessions indicate that the amaranth panel used in this study could be a rich source of genetic diversity for breeding purposes for drought tolerance traits.

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Estimation of drought tolerance among maize landraces from mini-core collection

Genetika ◽

10.2298/gensr1403775a ◽

2014 ◽

Vol 46 (3) ◽

pp. 775-788 ◽

Cited By ~ 1

Author(s):

Violeta Andjelkovic ◽

Natalija Kravic ◽

Vojka Babic ◽

Dragana Ignjatovic-Micic ◽

Zoran Dumanovic ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Grain Yield ◽

Stress Tolerance ◽

Core Collection ◽

High Density ◽

Tolerance Index ◽

Mini Core Collection ◽

Drought Tolerant ◽

Maize Landraces

Global climate change, its impact on stable food production in the future and possibilities to overcome the problem are the major priorities for research. Breeding varieties with increase adaptability to changing environments, together with better tolerance/resistance to abiotic stress, pest and diseases are possible solution. Maize is one of the most important crops, with high grain yield reduction induced by drought stress. In the present study twenty-six maize landraces from drought tolerant mini-core collection were tested under optimal, drought, and a combination of drought and high density stresses in the field. Morphological traits, plant height, total number of leaves, leaf length, leaf width, anthesis-silking interval and grain yield were recorded for each entry in two replications in three experiments. Besides, drought tolerant indices were evaluated to test the ability to separate more drought tolerant accessions from those with less stress tolerance. Five stress tolerance indices, including stress tolerance index (STI), mean productivity (MP), geometric mean productivity (GMP), stress susceptibility (SSI), and stress tolerance (TOL) were calculated. Data analyses revealed that STI, MP and GMP had positive and significant correlations with grain yield under all conditions. Three-dimensional diagrams displayed assignment of landraces L25, L1, L14, L3, L26, L15 and L16 to group A, based on the stress tolerance index and achieved grain yield under optimal, drought stress, and a combination of drought and high density stress. A biplot analysis efficiently separated groups of landraces with different level of drought tolerance and grain yield. Based on all obtained results, maize landraces L25, L14, L1 and L3, as the most valuable source of drought tolerance, could be recommended for further use in breeding programs.

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Drought Affected Wheat Production in Bangladesh and Breeding Strategies for Drought Tolerance

10.5772/intechopen.95283 ◽

2021 ◽

Author(s):

Afsana Hannan ◽

Md. Najmol Hoque ◽

Lutful Hassan ◽

Arif Hasan Khan Robin

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Drought Event ◽

Breeding Strategies ◽

Wheat Varieties ◽

Wheat Production ◽

Drought Tolerant ◽

Effects Of Drought

Wheat is one of the major cereal crops in Bangladesh. Over the last two decades, wheat consumption has passionately amplified in Bangladesh but its production has declined due to various stress environments. Recurrent drought event due to climate change that threatens the country’s food safety has become a serious concern. To safeguard the food security, adopting suitable breeding strategies can add momentum. Developing drought tolerant wheat varieties are the definitive means of protecting the crop against hostile effects of drought. Plant breeders are exploring various breeding strategies to breed for the varieties that can cope with water deficient conditions well. Besides, breeders are consistently looking for new prospects and strategies that can boost genetic gain in yield. To endorse drought tolerance in wheat, understanding the physiological and genetic adaptation mechanisms of wheat cultivars during drought stress would provide the estimated benchmarks to adjust for suitable breeding programs. The efforts of developing drought tolerant wheat genotypes could be supported by different breeding strategies including in vitro haploid and double haploid protocols, polyploidization, development of various types of hybrids and induced mutants by utilizing both classical and molecular breeding techniques. The proposed book chapter shall discuss the pattern of drought-stress in the wheat growing regions, effects of drought stress on wheat production and suitable breeding strategies for developing drought tolerant genotypes in Bangladesh.

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Improving Drought Tolerance in Mungbean (Vigna radiata L. Wilczek): Morpho-Physiological, Biochemical and Molecular Perspectives

Agronomy ◽

10.3390/agronomy11081534 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1534

Author(s):

Chandra Mohan Singh ◽

Poornima Singh ◽

Chandrakant Tiwari ◽

Shalini Purwar ◽

Mukul Kumar ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Crop Production ◽

Mitigation Strategies ◽

Theoretical Research ◽

Whole Genome Sequence ◽

Complex Nature ◽

Drought Tolerant ◽

Breeding Programmes ◽

The Impact

Drought stress is considered a severe threat to crop production. It adversely affects the morpho-physiological, biochemical and molecular functions of the plants, especially in short duration crops like mungbean. In the past few decades, significant progress has been made towards enhancing climate resilience in legumes through classical and next-generation breeding coupled with omics approaches. Various defence mechanisms have been reported as key players in crop adaptation to drought stress. Many researchers have identified potential donors, QTLs/genes and candidate genes associated to drought tolerance-related traits. However, cloning and exploitation of these loci/gene(s) in breeding programmes are still limited. To bridge the gap between theoretical research and practical breeding, we need to reveal the omics-assisted genetic variations associated with drought tolerance in mungbean to tackle this stress. Furthermore, the use of wild relatives in breeding programmes for drought tolerance is also limited and needs to be focused. Even after six years of decoding the whole genome sequence of mungbean, the genome-wide characterization and expression of various gene families and transcriptional factors are still lacking. Due to the complex nature of drought tolerance, it also requires integrating high throughput multi-omics approaches to increase breeding efficiency and genomic selection for rapid genetic gains to develop drought-tolerant mungbean cultivars. This review highlights the impact of drought stress on mungbean and mitigation strategies for breeding high-yielding drought-tolerant mungbean varieties through classical and modern omics technologies.

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Drought Tolerant near Isogenic Lines (NILs) of Pusa 44 Developed through Marker Assisted Introgression of qDTY2.1 and qDTY3.1 Enhances Yield under Reproductive Stage Drought Stress

Agriculture ◽

10.3390/agriculture11010064 ◽

2021 ◽

Vol 11 (1) ◽

pp. 64

Author(s):

Priyanka Dwivedi ◽

Naleeni Ramawat ◽

Gaurav Dhawan ◽

Subbaiyan Gopala Krishnan ◽

Kunnummal Kurungara Vinod ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Grain Quality ◽

Reproductive Stage ◽

Recurrent Parent ◽

Near Isogenic Lines ◽

Improvement Project ◽

Isogenic Lines ◽

Drought Tolerant ◽

Rice Improvement

Reproductive stage drought stress (RSDS) is detrimental for rice, which affects its productivity as well as grain quality. In the present study, we introgressed two major quantitative trait loci (QTLs), namely, qDTY2.1 and qDTY3.1, governing RSDS tolerance in a popular high yielding non-aromatic rice cultivar, Pusa 44, through marker-assisted backcross breeding (MABB). Pusa 44 is highly sensitive to RSDS, which restricts its cultivation across drought-prone environments. Foreground selection was carried out using markers, RM520 for qDTY3.1 and RM 521 for qDTY2.1. Background selection was achieved with 97 polymorphic SSR markers in tandem with phenotypic selection to achieve faster recurrent parent genome (RPG) recovery. Three successive backcrosses followed by three selfings aided RPG recoveries of 98.6% to 99.4% among 31 near isogenic lines (NILs). Fourteen NILs were found to be significantly superior in yield and grain quality under RSDS with higher drought tolerance efficiency (DTE) than Pusa 44. Among these, the evaluation of two promising NILs in the multilocational trial during Kharif 2019 showed that they were significantly superior to Pusa 44 under reproductive stage drought stress, while performing on par with Pusa 44 under normal irrigated conditions. These di-QTL pyramided drought-tolerant NILs are in the final stages of testing the All India Coordinated Rice Improvement Project varietal trials for cultivar release. Alternately, the elite drought-tolerant Pusa 44 NILs will serve as an invaluable source of drought tolerance in rice improvement.

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Genetic Diversity of Selected Rice Genotypes under Water Stress Conditions

Plants ◽

10.3390/plants10010027 ◽

2020 ◽

Vol 10 (1) ◽

pp. 27

Author(s):

Mahmoud M. Gaballah ◽

Azza M. Metwally ◽

Milan Skalicky ◽

Mohamed M. Hassan ◽

Marian Brestic ◽

...

Keyword(s):

Genetic Diversity ◽

Water Stress ◽

Drought Stress ◽

Drought Tolerance ◽

Ssr Markers ◽

Similarity Index ◽

Stress Conditions ◽

Yield Potential ◽

Drought Tolerant ◽

Rice Genotypes

Drought is the most challenging abiotic stress for rice production in the world. Thus, developing new rice genotype tolerance to water scarcity is one of the best strategies to achieve and maximize high yield potential with water savings. The study aims to characterize 16 rice genotypes for grain and agronomic parameters under normal and drought stress conditions, and genetic differentiation, by determining specific DNA markers related to drought tolerance using Simple Sequence Repeats (SSR) markers and grouping cultivars, establishing their genetic relationship for different traits. The experiment was conducted under irrigated (normal) and water stress conditions. Mean squares due to genotype × environment interactions were highly significant for major traits. For the number of panicles/plants, the genotypes Giza179, IET1444, Hybrid1, and Hybrid2 showed the maximum mean values. The required sterility percentage values were produced by genotypes IET1444, Giza178, Hybrid2, and Giza179, while, Sakha101, Giza179, Hybrid1, and Hybrid2 achieved the highest values of grain yield/plant. The genotypes Giza178, Giza179, Hybrid1, and Hybrid2, produced maximum values for water use efficiency. The effective number of alleles per locus ranged from 1.20 alleles to 3.0 alleles with an average of 1.28 alleles, and the He values for all SSR markers used varied from 0.94 to 1.00 with an average of 0.98. The polymorphic information content (PIC) values for the SSR were varied from 0.83 to 0.99, with an average of 0.95 along with a highly significant correlation between PIC values and the number of amplified alleles detected per locus. The highest similarity coefficient between Giza181 and Giza182 (Indica type) was observed and are susceptible to drought stress. High similarity percentage between the genotypes (japonica type; Sakha104 with Sakha102 and Sakha106 (0.45), Sakha101 with Sakha102 and Sakha106 (0.40), Sakha105 with Hybrid1 (0.40), Hybrid1 with Giza178 (0.40) and GZ1368-S-5-4 with Giza181 (0.40)) was also observed, which are also susceptible to drought stress. All genotypes are grouped into two major clusters in the dendrogram at 66% similarity based on Jaccard’s similarity index. The first cluster (A) was divided into two minor groups A1 and A2, in which A1 had two groups A1-1 and A1-2, containing drought-tolerant genotypes like IET1444, GZ1386-S-5-4 and Hybrid1. On the other hand, the A1-2 cluster divided into A1-2-1 containing Hybrid2 genotype and A1-2-2 containing Giza179 and Giza178 at coefficient 0.91, showing moderate tolerance to drought stress. The genotypes GZ1368-S-5-4, IET1444, Giza 178, and Giza179, could be included as appropriate materials for developing a drought-tolerant variety breeding program. Genetic diversity to grow new rice cultivars that combine drought tolerance with high grain yields is essential to maintaining food security.

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C-Repeat Binding Factor and Dehydrin Genes are Induced Co-Ordinately in Drought Tolerance Response of Wheat Cultivars

Journal of Agricultural Science ◽

10.5539/jas.v9n2p18 ◽

2017 ◽

Vol 9 (2) ◽

pp. 18 ◽

Cited By ~ 1

Author(s):

Csilla Deák ◽

Katalin Jäger ◽

Veronika Anna Nagy ◽

Réka Oszlányi ◽

Beáta Barnabás ◽

...

Keyword(s):

Drought Tolerance ◽

Mrna Level ◽

Drought Treatment ◽

Drought Tolerant ◽

Aba Sensitivity ◽

Defence Genes ◽

Binding Factor ◽

Tolerance Response ◽

Pcr Method

Four bread wheat genotypes with contrasting drought stress tolerance were studied. Expression levels of dehydrin (Wdhn13) and C-repeat binding factor (Cbf14, Cbf15) genes were investigated in leaves of two drought tolerant (Plainsman V, Mv Emese) and two sensitive (GK Élet, Cappelle Desprez) cultivars by semi-quantitative RT-PCR during drought treatment at anthesis. Coordinate induction of Cbf14, Cbf15 and Wdhn13 genes occurred at a late stage of stress treatment in all cultivars except the most sensitive Cappelle Desprez, where no induction was evident. The most pronounced late induction of genes was observed in the tolerant Mv Emese genotype. Cbf14, Cbf15 and Wdhn13 showed largely parallel changes of expression in stressed adult plants. The mRNA level of the same set of genes was measured in leaves of non-stressed seedlings with qRT-PCR method. Expression level of Wdhn13 was high and low in seedlings of tolerant and sensitive cultivars, respectively. Cbf15 specific transcript was barely detectable in leaves of non-stressed seedlings. In order to shed light on any potential difference in hormone responsiveness, seedlings were subjected to ABA treatment in vitro. At low hormone concentrations (10 and 20 µM ABA) consistently weaker ABA induced root growth retardation of GK Élet was found in comparison with the other three cultivars. Results highlight pronounced and late induction of a set of defence genes and low ABA sensitivity as features appearing in drought tolerant and sensitive responses, respectively. Data is discussed in the light of multifactorial determination of the complex phenotype of drought tolerance in wheat.

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Drought Stress in Grain Legumes: Effects, Tolerance Mechanisms and Management

Agronomy ◽

10.3390/agronomy11122374 ◽

2021 ◽

Vol 11 (12) ◽

pp. 2374

Author(s):

Marium Khatun ◽

Sumi Sarkar ◽

Farzana Mustafa Era ◽

A. K. M. Mominul Islam ◽

Md. Parvez Anwar ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Association Studies ◽

Stomatal Closure ◽

Grain Legumes ◽

Grain Legume ◽

Developmental Cycle ◽

Genome Wide Association Studies ◽

Tolerance Mechanisms ◽

Drought Tolerant

Grain legumes are important sources of proteins, essential micronutrients and vitamins and for human nutrition. Climate change, including drought, is a severe threat to grain legume production throughout the world. In this review, the morpho-physiological, physio-biochemical and molecular levels of drought stress in legumes are described. Moreover, different tolerance mechanisms, such as the morphological, physio-biochemical and molecular mechanisms of legumes, are also reviewed. Moreover, various management approaches for mitigating the drought stress effects in grain legumes are assessed. Reduced leaf area, shoot and root growth, chlorophyll content, stomatal conductance, CO2 influx, nutrient uptake and translocation, and water-use efficiency (WUE) ultimately affect legume yields. The yield loss of grain legumes varies from species to species, even variety to variety within a species, depending upon the severity of drought stress and several other factors, such as phenology, soil textures and agro-climatic conditions. Closure of stomata leads to an increase in leaf temperature by reducing the transpiration rate, and, so, the legume plant faces another stress under drought stress. The biosynthesis of reactive oxygen species (ROS) is the most detrimental effect of drought stress. Legumes can adapt to the drought stress by changing their morphology, physiology and molecular mechanism. Improved root system architecture (RSA), reduced number and size of leaves, stress-induced phytohormone, stomatal closure, antioxidant defense system, solute accumulation (e.g., proline) and altered gene expression play a crucial role in drought tolerance. Several agronomic, breeding both conventional and molecular, biotechnological approaches are used as management practices for developing a drought-tolerant legume without affecting crop yield. Exogenous application of plant-growth regulators (PGRs), osmoprotectants and inoculation by Rhizobacteria and arbuscular mycorrhizal fungi promotes drought tolerance in legumes. Genome-wide association studies (GWASs), genomic selection (GS), marker-assisted selection (MAS), OMICS-based technology and CRISPR/Cas9 make the breeding work easy and save time in the developmental cycle to get resistant legumes. Several drought-resistant grain legumes, such as the chickpea, faba bean, common bean and pigeon pea, were developed by different institutions. Drought-tolerant transgenic legumes, for example, chickpeas, are developed by introgressing desired genes through breeding and biotechnological approaches. Several quantitative trait loci (QTLs), candidate genes occupying drought-tolerant traits, are identified from a variety of grain legumes, but not all are under proper implementation. Hence, more research should be conducted to improve the drought-tolerant traits of grain legumes for avoiding losses during drought.

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Discrimination of drought tolerance in a worldwide collection of safflower (Carthamus tinctorius L.) genotypes based on selection indices

Acta agriculturae Slovenica ◽

10.14720/aas.2021.117.1.1656 ◽

2021 ◽

Vol 117 (1) ◽

pp. 1

Author(s):

Pooran GOLKAR ◽

Esmaeil HAMZEH ◽

Seyed Ali Mohammad MIRMOHAMMADY MAIBODY

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Geometric Mean ◽

Resistance Index ◽

Stability Index ◽

Carthamus Tinctorius ◽

Tolerance Index ◽

Breeding Programs ◽

Drought Tolerance Indices ◽

Tolerance Indices

Improvement of elite safflower genotypes for drought-tolerance is hampered by a deficiency of effective selection criteria. The present study evaluated 100 genotypes of safflower in terms of their drought tolerance over a period of three years (2016–2018) under both non-stress and drought-stress conditions. The eight drought-tolerance indices of tolerance index (TOL), mean productivity (MP), geometric mean productivity (GMP), stress susceptibility index (SSI), stress tolerance index (STI), yield stability index (YSI), drought resistance index (DI), and harmonic mean (HARM) were calculated based on seed yield under drought (Ys) and non-drought (Yp) conditions. A high genetic variation was found in drought tolerance among the genotypes studied. The MP, GMP, and STI indices were able to discriminate between tolerant and drought-sensitive genotypes. Plots of the first and second principal components identified drought-tolerant genotypes averaged over the three study years. Cluster analysis divided the genotypes into three distinct groups using the drought tolerance indices. Ultimately, eight genotypes (namely, G3, G11, G13, G24, G33, G47, G58, and G61) from different origins were detected as more tolerant to drought stress suitable for use in safflower breeding programs in drought-affected areas. The most tolerant and susceptible genotypes could be exploited to produce mapping populations for drought tolerance breeding programs in safflower.

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Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.6.1.20-28 ◽

2015 ◽

Vol 6 (1) ◽

pp. 20

Author(s):

Erna Sinaga ◽

Megayani Sri Rahayu ◽

Awang Maharijaya

Keyword(s):

Tissue Culture ◽

Drought Tolerance ◽

In Vitro Selection ◽

Solanum Melongena ◽

Survival Percentage ◽

Drought Tolerant ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design

ABSTRACTThe objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought tolerance of eggplant and the drought tolerant eggplant accessions. The experiment was conducted at the Laboratory of Tissue Culture, Department of Agronomy and Horticulture, Bogor Agricultural University. The experiment was arranged in a completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10, and 15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090). The results showed that the addition of PEG to in vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought ABSTRAKPenelitian ini bertujuan untuk mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG) terhadap pertumbuhan tanaman terung in vitro, mendapatkan konsentrasi PEG yang dapat digunakan untuk seleksi tanaman terung secara in vitro dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan, Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor. Penelitian ini disusun dalam rancangan acak lengkap dua faktor. Faktor pertama adalah konsentrasi PEG terdiri atas 0, 5, 10, dan 15%. Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090). Hasil penelitian menunjukkan bahwa penambahan PEG pada media in vitro memberikan pengaruh nyata dan sangat nyata terhadap persentase hidup eksplan, persentase eksplan berkalus, pertambahan tinggi tunas, dan jumlah daun tanaman terung. Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan

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