Effects of in ovo Inoculation of Multi-Strain Lactobacilli on Cytokine Gene Expression and Antibody-Mediated Immune Responses in Chickens

Abstract Senior horses often exhibit chronic inflammation and decreased immune responses. Dietary Saccharomyces cerevisiae fermentation product (SCFP; Diamond V, Cedar Rapids, IA) has been shown to affect immune responses in several species. This study aimed to evaluate SCFP-mediated immune function in senior horses. Sixteen horses (24.8 ± 3.0 y; BW = 545.8 ± 61.9 kg) were allotted to two treatments: CON (n = 8; no SCFP supplementation) and SCFP top dressed onto a common concentrate for 56 d (21 g/d; n = 8). Body condition score (BCS), BW, whole blood cytokine (INF-ɣ, TNF-α, IL-1β, IL-6, IL-10, IL-4, IL-8, IL-13, IL-17) and COX-1 and COX-2 gene expression were measured at d 0, 42, 49 and 56. Horses were challenged with a monovalent influenza vaccine at d 42 (MIV; Fluvacc Innovator; Zoetis Animal Health, Parsippany, NJ). Pre-MIV (d 0–42) and post-MIV (d 42–56) responses were analyzed using general mixed model procedures. Pre-MIV, BW tended to increase (P = 0.09) over time. Expression of IL-10 tended to be lower for SCFP than CON (P = 0.09) and IL-13 expression decreased over time (P < 0.05). Post-MIV, BCS linearly increased over time (P = 0.006) while TNF-α tended to increase at d 49 and return to d 42 levels by d 56 (P = 0.06). Both INF-ɣ and IL-10 expression were lower for SCFP vs CON (P < 0.05). Gene expression of COX-2 tended to decrease (P = 0.06) at d 49 and return to the d 42 baseline by d 56. Gene expression of IL-13 tended to decrease with SCFP but increased with CON at d 49 with both returning to d 42 levels by d 56 (P = 0.08). Influenza H1 titers increased over time post-vaccination (P < 0.001) with no difference between treatments. Dietary SCFP may modulate pro-inflammatory and anti-inflammatory cytokine gene expression in senior horses.

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Modulation of amplitude and direction ofin vivo immune responses by co-administration of cytokine gene expression cassettes with DNA immunogens

European Journal of Immunology ◽

10.1002/(sici)1521-4141(199803)28:03<1089::aid-immu1089>3.0.co;2-l ◽

1998 ◽

Vol 28 (3) ◽

pp. 1089-1103 ◽

Cited By ~ 167

Author(s):

Jong J. Kim ◽

Neil N. Trivedi ◽

Liesl K. Nottingham ◽

Lake Morrison ◽

Anthony Tsai ◽

...

Keyword(s):

Gene Expression ◽

Immune Responses ◽

Cytokine Gene Expression ◽

Cytokine Gene

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Development of a cytokine gene expression assay for the relative quantification of the African elephant (Loxodonta africana) cell-mediated immune responses

Cytokine ◽

10.1016/j.cyto.2021.155453 ◽

2021 ◽

Vol 141 ◽

pp. 155453

Author(s):

Candice R. de Waal ◽

Léanie Kleynhans ◽

Sven D.C. Parsons ◽

Wynand J. Goosen ◽

Guy Hausler ◽

...

Keyword(s):

Gene Expression ◽

Immune Responses ◽

Loxodonta Africana ◽

African Elephant ◽

Cytokine Gene Expression ◽

Relative Quantification ◽

Cytokine Gene ◽

Gene Expression Assay

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In Ovo and Oral Administration of Probiotic Lactobacilli Modulate Cell- and Antibody-Mediated Immune Responses in Newly Hatched Chicks

Frontiers in Immunology ◽

10.3389/fimmu.2021.664387 ◽

2021 ◽

Vol 12 ◽

Author(s):

Mohammadali Alizadeh ◽

Jegarubee Bavananthasivam ◽

Bahram Shojadoost ◽

Jake Astill ◽

Khaled Taha-Abdelaziz ◽

...

Keyword(s):

Gene Expression ◽

Immune Response ◽

Immune Responses ◽

Oral Administration ◽

Keyhole Limpet Hemocyanin ◽

Bursa Of Fabricius ◽

Cytokine Gene Expression ◽

Composition Analysis ◽

In Ovo ◽

Probiotic Lactobacilli

There is some evidence that lactobacilli can strengthen the immune system of chickens. This study evaluated the effects of in ovo and oral administration of a lactobacilli cocktail on cytokine gene expression, antibody-mediated immune responses, and spleen cellularity in chickens. Lactobacilli were administered either in ovo at embryonic day 18, orally at days 1, 7, 14, 21, and 28 post-hatches, or a combination of both in ovo and post-hatch inoculation. On day 5 and 10 post-hatch, spleen and bursa of Fabricius were collected for gene expression and cell composition analysis. On days 14 and 21 post-hatch, birds were immunized with sheep red blood cells (SRBC) and keyhole limpet hemocyanin (KLH), and sera were collected on days 7, 14, and 21 post-primary immunization. Birds that received lactobacilli (107 CFU) via in ovo followed by weekly oral administration showed a greater immune response by enhancing antibody responses, increasing the percentage of CD4+ and CD4+CD25+ T cells in the spleen and upregulating the expression of interferon (IFN)-α, IFN-β, interleukin (IL)-8, IL-13, and IL-18 in the spleen and expression of IFN-γ, IL-2, IL-6, IL-8, IL-12, and IL-18 in the bursa. These findings suggest that pre-and post-hatch administration of lactobacilli can modulate the immune response in newly hatched chickens.

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