scholarly journals Diversity of the Swine Leukocyte Antigen Class I and II in Commercial Pig Populations

2021 ◽  
Vol 8 ◽  
Author(s):  
Navapon Techakriengkrai ◽  
Teerawut Nedumpun ◽  
William T. Golde ◽  
Sanipa Suradhat
Keyword(s):  
Immune Responses ◽  
Reproductive Performance ◽  
Genetic Improvement ◽  
Class I ◽  
Swine Leukocyte Antigen ◽  
Swine Production ◽  
Breeding Scheme ◽  
Leukocyte Antigen ◽  
Swine Population ◽  
The Common

Among swine genetic markers, the highly polymorphic swine leukocyte antigen (SLA) is one of the key determinants, associated with not only immune responses but also reproductive performance and meat quality. The objective of this study was to characterize the SLA class I and II diversities in the commercial pig populations. In this study, a total number of 158 pigs (126 gilts and 32 boars) were randomly selected from different breeding herds of five major pig-producing companies, which covered ~70% of Thai swine production. The results indicate that a moderate level of SLA diversity was maintained in the Thai swine population, despite the performance-oriented breeding scheme. The highly common SLA class I alleles were SLA-1*08:XX, SLA-2*02:XX, and SLA-3*04:XX at a combined frequency of 30.1, 18.4, and 34.5%, respectively, whereas DRB1*04:XX, DQB1*02:XX and DQA*02:XX were the common class II alleles at 22.8, 33.3, and 38.6%, respectively. The haplotype Lr-32.0 (SLA-1*07:XX, SLA-2*02:XX, and SLA-3*04:XX) and Lr-0.23 (DRB1*10:XX, DQB1*06:XX, DQA* 01:XX) was the most common SLA class I and II haplotype, at 15.5 and 14.6%, respectively. Common class I and II haplotypes were also observed, which Lr-22.15 was the most predominant at 11.1%, followed by Lr-32.12 and Lr-4.2 at 10.8 and 7.9%, respectively. To our knowledge, this is the first report of SLA class I and II diversities in the commercial pigs in Southeast Asia. The information of the common SLA allele(s) in the population could facilitate swine genetic improvement and future vaccine design.

scholarly journals Phylogenic analysis of the SLA-1 gene variants observed from Korean Jeju pig (Brief Report)

2007 ◽  
Vol 50 (6) ◽  
pp. 628-629
Author(s):  
H. Chung ◽  
S. Yoon ◽  
M. Beck ◽  
H. Park ◽  
J. Kim
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Immune Responses ◽  
Class I ◽  
Gene Variants ◽  
Nucleotide Polymorphisms ◽  
Swine Leukocyte Antigen ◽  
Single Nucleotide ◽  
Phylogenic Analysis ◽  
Leukocyte Antigen ◽  
Miniature Pigs

Abstract. The Swine Leukocyte Antigen (SLA) class I consists of SLA-1, SLA-2, and SLA-3 genes (CHARON et al., 2000) that are expressed on the surface of most nucleated cells. Pig organs mainly from miniature pigs have been used in studies on xenotransplantation (SMITH et al., 2005). It is important to understand genetic variations of the SLA-1 gene considering the SLA-mediated immune responses. Collecting information on single nucleotide polymorphisms (SNPs) and alleles of SLA-1 and their phylogenic relationships is important regarding future uses of the Korean Jeju Pig (KJP) in xenotransplantation.

A NONCLASSICAL CLASS I SWINE LEUKOCYTE ANTIGEN (PD-6) FUNCTION AS A NOVEL PORCINE NATURAL KILLER CELL INHIBITORY MOLECULE.

2000 ◽  
Vol 69 (Supplement) ◽  
pp. S384
Author(s):  
Hitomi Sasaki ◽  
Xiao-Chun Xu ◽  
Bashoo Naziruddin ◽  
Toru Higuchi ◽  
Douglas M. Smith ◽  
...  
Keyword(s):  
Natural Killer Cell ◽  
Natural Killer ◽  
Killer Cell ◽  
Class I ◽  
Swine Leukocyte Antigen ◽  
Leukocyte Antigen ◽  
Inhibitory Molecule ◽  
Nonclassical Class

scholarly journals HLA tapasin independence: broader peptide repertoire and HIV control

2020 ◽  
Vol 117 (45) ◽  
pp. 28232-28238 ◽  
Author(s):  
Arman A. Bashirova ◽  
Mathias Viard ◽  
Vivek Naranbhai ◽  
Alba Grifoni ◽  
Wilfredo Garcia-Beltran ◽  
...  
Keyword(s):  
Immune Responses ◽  
Ex Vivo ◽  
Human Leukocyte ◽  
Hla Class I ◽  
Class I ◽  
Cytotoxic T Cell ◽  
Leukocyte Antigen ◽  
Viral Loads ◽  
Peptide Loading ◽  
Cytotoxic T Cell Responses

Human leukocyte antigen (HLA) class I allotypes vary in their ability to present peptides in the absence of tapasin, an essential component of the peptide loading complex. We quantified tapasin dependence of all allotypes that are common in European and African Americans (n= 97), which revealed a broad continuum of values. Ex vivo examination of cytotoxic T cell responses to the entire HIV-1 proteome from infected subjects indicates that tapasin-dependent allotypes present a more limited set of distinct peptides than do tapasin-independent allotypes, data supported by computational predictions. This suggests that variation in tapasin dependence may impact the strength of the immune responses by altering peptide repertoire size. In support of this model, we observed that individuals carryingHLA class Igenotypes characterized by greater tapasin independence progress more slowly to AIDS and maintain lower viral loads, presumably due to increased breadth of peptide presentation. Thus, tapasin dependence level, likeHLAzygosity, may serve as a means to restrict or expand breadth of the HLA-I peptide repertoire across humans, ultimately influencing immune responses to pathogens and vaccines.

scholarly journals Difference in number of loci of swine leukocyte antigen classical class I genes among haplotypes

Genomics ◽  
2009 ◽  
Vol 93 (3) ◽  
pp. 261-273 ◽  
Author(s):  
Maiko Tanaka-Matsuda ◽  
Asako Ando ◽  
Claire Rogel-Gaillard ◽  
Patrick Chardon ◽  
Hirohide Uenishi
Keyword(s):  
Class I ◽  
Swine Leukocyte Antigen ◽  
Leukocyte Antigen ◽  
Classical Class

scholarly journals Targeting Swine Leukocyte Antigen Class I Molecules for Proteasomal Degradation by the nsp1α Replicase Protein of the Chinese Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Strain JXwn06

2015 ◽  
Vol 90 (2) ◽  
pp. 682-693 ◽  
Author(s):  
Jige Du ◽  
Xinna Ge ◽  
Ying Liu ◽  
Ping Jiang ◽  
Zhe Wang ◽  
...  
Keyword(s):  
Proteasomal Degradation ◽  
Class I ◽  
Respiratory Syndrome Virus ◽  
Swine Leukocyte Antigen ◽  
Highly Pathogenic ◽  
Leukocyte Antigen ◽  
Low Level ◽  
Replicase Protein ◽  
Ctl Responses ◽  
Leukocyte Antigen Class I

ABSTRACTPorcine reproductive and respiratory syndrome virus (PRRSV) is a critical pathogen of swine, and infections by this virus often result in delayed, low-level induction of cytotoxic T lymphocyte (CTL) responses in pigs. Here, we report that a Chinese highly pathogenic PRRSV strain possessed the ability to downregulate swine leukocyte antigen class I (SLA-I) molecules on the cell surface of porcine alveolar macrophages and target them for degradation in a manner that was dependent on the ubiquitin-proteasome system. Moreover, we found that the nsp1α replicase protein contributed to this property of PRRSV. Further mutagenesis analyses revealed that this function of nsp1α required the intact molecule, including the zinc finger domain, but not the cysteine protease activity. More importantly, we found that nsp1α was able to interact with both chains of SLA-I, a requirement that is commonly needed for many viral proteins to target their cellular substrates for proteasomal degradation. Together, our findings provide critical insights into the mechanisms of how PRRSV might evade cellular immunity and also add a new role for nsp1α in PRRSV infection.IMPORTANCEPRRSV infections often result in delayed, low-level induction of CTL responses in pigs. Deregulation of this immunity is thought to prevent the virus from clearance in an efficient and timely manner, contributing to persistent infections in swineherds. Our studies in this report provide critical insight into the mechanism of how PRRSV might evade CTL responses. In addition, our findings add a new role for nsp1α, a critical viral factor involved in antagonizing host innate immunity.

Molecular characterization of swine leukocyte antigen class I genes by sequence-based and PCR-SSP method in Guizhou minipigs

2014 ◽  
Vol 41 (12) ◽  
pp. 7775-7782 ◽  
Author(s):  
Jihan Xia ◽  
Zizhan Liu ◽  
Leilei Xin ◽  
Zaigui Wang ◽  
Lin Qian ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Class I ◽  
Swine Leukocyte Antigen ◽  
Leukocyte Antigen ◽  
Leukocyte Antigen Class I
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